nunc immuno microwell 96 well plates  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher nunc immuno microwell 96 well plates
    Nunc Immuno Microwell 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc immuno microwell 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc immuno microwell 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

    Images

    Related Articles

    Cell Isolation:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CD4+ T cells were purified by MACS technology using MagCellect rat CD4+ T Cell Isolation Kit (R & D Systems, Minneapolis, USA) according to the manufacturer’s instructions. .. CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb.

    Mass Spectrometry:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc. .. After stratification at 4°C in darkness for 2 d, seeds were placed in a growth chamber (BrightBoy GroBank, cat. no. BB-XXL; Clf Plant Climatics, Wertingen, Germany) and grown at 26°C grown under long d conditions (16 h, 80 μ m m−2 s−1 cool-white light/8 h dark).

    Cytometry:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb. .. After 65–72 h incubation, cells were washed in PBS, collected and the absolute amount of proliferating rat T cells was determined by flow cytometry.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
    Article Snippet: .. Final ELISA Testing Procedure In brief, viral protein was coated into 96-well Nunc MicroWellTM plates (Thermo Fisher, US) by diluting the stock to 0.75 µg/mL in NaCO3 buffer (0.1 M, PH 9.6) and coating with 50 µL per well with overnight incubation at 4 °C. .. The plates were washed 5 times with 0.1% PBS-T then blocked with 5% milk powder (Carl Roth, Germany) in PBS-T for one hour at room temperature and the wash repeated.

    Microarray:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc. .. After stratification at 4°C in darkness for 2 d, seeds were placed in a growth chamber (BrightBoy GroBank, cat. no. BB-XXL; Clf Plant Climatics, Wertingen, Germany) and grown at 26°C grown under long d conditions (16 h, 80 μ m m−2 s−1 cool-white light/8 h dark).

    Incubation:

    Article Title: Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks
    Article Snippet: MTT assay M21 and HT29 cells were seeded in 96-well microtiter plates (Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Polymer Base) at 1 × 103 and 4 × 103 cells per well, respectively, in 100 μL of medium. .. Plates were incubated for 24 h. Medium was replaced with medium containing escalating concentrations of drugs and the plates were incubated for 96 h. Afterwards, 10 μL of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (Thiazol Blue Tetrazolium Bromide 98% - M2128 - SIGMA) (5 mg/mL in water) were added to the wells.

    Article Title: Saliva-Induced Clotting Captures Streptococci: Novel Roles for Coagulation and Fibrinolysis in Host Defense and Immune Evasion
    Article Snippet: .. Coagulation of normal plasma without anticoagulant was performed in microwell plates (Nunclon Delta Surface; Nunc A/S), and the absorbance at 490 nm was measured over time during incubation at 37°C. .. To all samples, 300 μg of human fibrinogen (Sigma) was added to amplify the signal.

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb. .. After 65–72 h incubation, cells were washed in PBS, collected and the absolute amount of proliferating rat T cells was determined by flow cytometry.

    Article Title: Development of a Whole-Virus ELISA for Serological Evaluation of Domestic Livestock as Possible Hosts of Human Coronavirus NL63
    Article Snippet: .. Final ELISA Testing Procedure In brief, viral protein was coated into 96-well Nunc MicroWellTM plates (Thermo Fisher, US) by diluting the stock to 0.75 µg/mL in NaCO3 buffer (0.1 M, PH 9.6) and coating with 50 µL per well with overnight incubation at 4 °C. .. The plates were washed 5 times with 0.1% PBS-T then blocked with 5% milk powder (Carl Roth, Germany) in PBS-T for one hour at room temperature and the wash repeated.

    Proliferation Assay:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: Paragraph title: T cell proliferation assay ... CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb.

    Expressing:

    Article Title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
    Article Snippet: .. The cell suspensions of eGFP expressing strains were centrifuged and washed in an equal amount of water before fluorescence microscopy (Leica Microsystems, Germany, DM LB2, DFC350FX) at 1,000-fold magnification, fluorescence images were taken using filter set ‘I3’ [excitation filter BP 450–490]. eGFP fluorescence (ex/em 488/507 nm) and OD600 were measured in a Synergy MX plate reader (Biotek, Winooski, VT, USA) using micro titer plates (Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base, Black; Thermo Fisher Scientific). ..

    Modification:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: Cell culture experiments Human embryonic kidney (HEK293.EBNA) cells (Life Technologies GmbH, Darmstadt, Germany) were maintained in high glucose Dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Sigma-Aldrich Chemie GmbH, Munich, Germany [F7524, Lot#124M3337]) and 1% penicillin/streptomycin 10,000 U/mL (Gibco by Life Technologies GmbH, Darmstadt, Germany). .. For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess.

    Western Blot:

    Article Title: C-terminal calcium binding of α-synuclein modulates synaptic vesicle interaction
    Article Snippet: .. Cells were plated at 5000 cells/well in Nunc MicroWell 96-well plates (Thermo Fisher Scientific) for cytotoxicity assays and at 700,000 cells/dish in 48 mm dishes (Nunc A/A) for western blotting studies. ..

    Transfection:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: .. For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess. .. To deliver plasmid DNA we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA:PEI40).

    Cell Culture:

    Article Title: Levels of circulating myeloid subpopulations and of heme oxygenase-1 do not predict CD4+ T cell recovery after the initiation of antiretroviral therapy for HIV disease
    Article Snippet: .. PBMCs were cultured on Upcell™ 96 F MicroWell plates (Nalge Nunc, Rochester, NY), either with saline or 25 μM CoPP in R10 at 37°C for 48 hours. .. Flow cytometry antibody labeling The monoclonal antibodies (mAbs) used in this study were purchased from Abcam (Cambridge, MA), BD Biosciences (Franklin Lakes, NJ), Beckman Coulter (Indianapolis, IN), BioLegend (San Diego, CA), eBiosciences (San Diego, CA), and Invitrogen (Carlsbad, CA), and are summarized in detail in Additional file : Table S1.

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: Paragraph title: Cell culture experiments ... For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess.

    Article Title: Alpha-synuclein fragments trigger distinct aggregation pathways
    Article Snippet: Paragraph title: Cell culture ... For experiments, cells were seeded on either T25 flasks or multi-well-plates (Nunc MicroWell plates, Thermo Fisher Scientific, Waltham, MA) sequentially coated with 50 µg/mL poly-l -ornithine (Sigma-Aldrich) and 5 µg/mL bovine fibronectin (Sigma-Aldrich).

    Polymerase Chain Reaction:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: Combinations of mutants and reporter lines were obtained by crossing individual lines and genotypes were verified phenotypically and by PCR genotyping. .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc.

    Magnetic Cell Separation:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CD4+ T cells were purified by MACS technology using MagCellect rat CD4+ T Cell Isolation Kit (R & D Systems, Minneapolis, USA) according to the manufacturer’s instructions. .. CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb.

    MTT Assay:

    Article Title: Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks
    Article Snippet: .. MTT assay M21 and HT29 cells were seeded in 96-well microtiter plates (Thermo Scientific™ Nunc™ MicroWell™ 96-Well Optical-Bottom Plates with Polymer Base) at 1 × 103 and 4 × 103 cells per well, respectively, in 100 μL of medium. .. Plates were incubated for 24 h. Medium was replaced with medium containing escalating concentrations of drugs and the plates were incubated for 96 h. Afterwards, 10 μL of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (Thiazol Blue Tetrazolium Bromide 98% - M2128 - SIGMA) (5 mg/mL in water) were added to the wells.

    Fluorescence:

    Article Title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
    Article Snippet: .. The cell suspensions of eGFP expressing strains were centrifuged and washed in an equal amount of water before fluorescence microscopy (Leica Microsystems, Germany, DM LB2, DFC350FX) at 1,000-fold magnification, fluorescence images were taken using filter set ‘I3’ [excitation filter BP 450–490]. eGFP fluorescence (ex/em 488/507 nm) and OD600 were measured in a Synergy MX plate reader (Biotek, Winooski, VT, USA) using micro titer plates (Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base, Black; Thermo Fisher Scientific). ..

    Article Title: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
    Article Snippet: .. Fluorescence reporter measurements and assays The fluorescence reporter and OD600 measurements were performed using 96-well microtiter plates (Nunc MicroWell 96-well optical-bottom plates with polymer base, black; Thermo Fisher Scientific) and a Synergy MX plate reader (Biotek, Winooski, VT, USA). ..

    Article Title: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
    Article Snippet: .. The fluorescence reporter and OD600 measurements were performed using 96-well microtiter plates (Nunc MicroWell 96-well optical-bottom plates with polymer base, black; Thermo Fisher Scientific) and a Synergy MX plate reader (Biotek, Winooski, VT, USA). ..

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: .. Viability and survival rate experiments In order to monitor BCG viability in the co-cultures, aliquots of 100 μl were transferred into Thermo Scientific Nunc MicroWell™ 96-well plates to measure fluorescence emission at 485/535 nm in a DTX 880 Multimode Detector microplate reader (Beckman Coulter). .. Simultaneously, BCG survival rate was determined by total bacterial counts on BCG-selective agar plates (Middlebrook 7H10 supplemented with 5% OADC and 0.2% glycerol).

    Isolation:

    Article Title: Lactic acid Bacteria isolated from European badgers (Meles meles) reduce the viability and survival of Bacillus Calmette-Guerin (BCG) vaccine and influence the immune response to BCG in a human macrophage model
    Article Snippet: Paragraph title: Isolation of LAB from faecal samples ... A total of 50 different colonies were then selected from MRS-agar plates containing approximately 20–100 colonies from each of the 26 collected faecal samples (a total of 1300 colonies), and cultivated in Thermo Scientific Nunc MicroWell™ 96-well plates with MRS broth at 37 °C for 48–60 h under microaerophilic conditions.

    Flow Cytometry:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: Seeds were surface-sterilized with 70% ethanol + 0.05% SDS for 3 min, rinsed with 96% ethanol for 1 min, and subsequently dried in a laminar flow hood. .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc.

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb. .. After 65–72 h incubation, cells were washed in PBS, collected and the absolute amount of proliferating rat T cells was determined by flow cytometry.

    Microscopy:

    Article Title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
    Article Snippet: .. The cell suspensions of eGFP expressing strains were centrifuged and washed in an equal amount of water before fluorescence microscopy (Leica Microsystems, Germany, DM LB2, DFC350FX) at 1,000-fold magnification, fluorescence images were taken using filter set ‘I3’ [excitation filter BP 450–490]. eGFP fluorescence (ex/em 488/507 nm) and OD600 were measured in a Synergy MX plate reader (Biotek, Winooski, VT, USA) using micro titer plates (Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base, Black; Thermo Fisher Scientific). ..

    Purification:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: CD4+ T cells were purified by MACS technology using MagCellect rat CD4+ T Cell Isolation Kit (R & D Systems, Minneapolis, USA) according to the manufacturer’s instructions. .. CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb.

    Transgenic Assay:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: Origin of transgenic lines: CYCB1;1::GUS (in Col-0) was provided by John Celenza ( ); CLV3::GUS and WUS::GUS (in L er ) were received from Thomas Laux ( ); pKLU::GUS (in L er ) was provided by Michael Lenhard ( ); and ARR5::GUS (in Col-0) was provided by Joe Kieber ( ). .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc.

    Protein Extraction:

    Article Title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
    Article Snippet: For intracellular HRP activity measurements, cells were broken using Yeast Protein Extraction Reagent (Y-PER from Thermo Scientific). .. The cell suspensions of eGFP expressing strains were centrifuged and washed in an equal amount of water before fluorescence microscopy (Leica Microsystems, Germany, DM LB2, DFC350FX) at 1,000-fold magnification, fluorescence images were taken using filter set ‘I3’ [excitation filter BP 450–490]. eGFP fluorescence (ex/em 488/507 nm) and OD600 were measured in a Synergy MX plate reader (Biotek, Winooski, VT, USA) using micro titer plates (Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base, Black; Thermo Fisher Scientific).

    Coagulation:

    Article Title: Saliva-Induced Clotting Captures Streptococci: Novel Roles for Coagulation and Fibrinolysis in Host Defense and Immune Evasion
    Article Snippet: .. Coagulation of normal plasma without anticoagulant was performed in microwell plates (Nunclon Delta Surface; Nunc A/S), and the absorbance at 490 nm was measured over time during incubation at 37°C. .. To all samples, 300 μg of human fibrinogen (Sigma) was added to amplify the signal.

    Labeling:

    Article Title: In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia
    Article Snippet: Labeling of cells was stopped by the addition of ice-cold complete IMDM medium and by three washing steps. .. CFSE-labeled and unlabeled CD4+ Rat T cells (1 × 105 cells/well) were seeded in 96-well microtiter plates (Nunc™ 96-Well Polystyrene Round Bottom Microwell Plates, Thermo Fisher Scientific, USA) in 100 μl medium with the addition of different concentrations of teriflunomide and stimulated with 3 μg/ml of plate-bound anti-CD3 mAb/anti-CD28 mAb.

    Plasmid Preparation:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: .. For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess. .. To deliver plasmid DNA we used the 40 kDa linear polyethylenimine (Polysciences Europe GmbH, Hirschberg, Germany) at a 1:3 ratio (DNA:PEI40).

    Concentration Assay:

    Article Title: Drug discovery with an RBM20 dependent titin splice reporter identifies cardenolides as lead structures to improve cardiac filling
    Article Snippet: For transfection 25000 cells/well were seeded on 96-well Nunc F96 MicroWell™ plates (Life Technologies GmbH, Darmstadt, Germany) and transfected with a total of 200 ng of plasmid DNA of which 1 ng was splice reporter plus a corresponding amount of RBM20, RBFOX1 or control plasmid (pcDNA3.1) in a 20x molar excess. .. Compounds were applied 24 h post-transfection at a final DMSO concentration of 1%.

    Activity Assay:

    Article Title: Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
    Article Snippet: Paragraph title: HRP activity assay, eGFP fluorescence microscopy and measurements ... The cell suspensions of eGFP expressing strains were centrifuged and washed in an equal amount of water before fluorescence microscopy (Leica Microsystems, Germany, DM LB2, DFC350FX) at 1,000-fold magnification, fluorescence images were taken using filter set ‘I3’ [excitation filter BP 450–490]. eGFP fluorescence (ex/em 488/507 nm) and OD600 were measured in a Synergy MX plate reader (Biotek, Winooski, VT, USA) using micro titer plates (Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base, Black; Thermo Fisher Scientific).

    Hood:

    Article Title: The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1The Small Molecule Hyperphyllin Enhances Leaf Formation Rate and Mimics Shoot Meristem Integrity Defects Associated with AMP1 Deficiency 1 [OPEN]
    Article Snippet: Seeds were surface-sterilized with 70% ethanol + 0.05% SDS for 3 min, rinsed with 96% ethanol for 1 min, and subsequently dried in a laminar flow hood. .. For the small-molecule screen, GUS-reporter assays, the microarray experiment, leaf formation rate, and shoot apical-meristem analyses, the sterile seeds were germinated and grown in 96-well plates (approximately 5 seeds per well; Nunclon Delta Surface, cat. no. 163320; Thermo Fisher Scientific/Nunc, Rochester, NY) in 100μ l liquid half-strength Murashige and Skoog medium (MS; Duchefa Biochemie, Haarlem, Netherlands) containing 1% Suc.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher 96 well nunc immuno microwell maxisorp plate
    96 Well Nunc Immuno Microwell Maxisorp Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/96 well nunc immuno microwell maxisorp plate/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    96 well nunc immuno microwell maxisorp plate - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher antibody enzyme linked immunosorbent assay elisa nunc immuno microwell 96 well plates
    Antibody Enzyme Linked Immunosorbent Assay Elisa Nunc Immuno Microwell 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody enzyme linked immunosorbent assay elisa nunc immuno microwell 96 well plates/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody enzyme linked immunosorbent assay elisa nunc immuno microwell 96 well plates - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    Thermo Fisher nunc immuno microwell 96 well plates
    Nunc Immuno Microwell 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc immuno microwell 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc immuno microwell 96 well plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    Image Search Results