nunc immuno maxisorp 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nunc immuno maxisorp 96 well plates
    Nunc Immuno Maxisorp 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc immuno maxisorp 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc immuno maxisorp 96 well plates - by Bioz Stars, 2020-04
    93/100 stars

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    Transfection:

    Article Title: Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries
    Article Snippet: For determination of antigen-binding, Nunc-Immuno MaxiSorp 96-well plates (Thermo Scientific) were coated with antigens diluted in coating buffer (100 mM bicarbonate/carbonate buffer), using previously determined limiting concentrations of antigen to minimize avidity effects (0.25 μg/ml Meso-strep, 0.125 μg/ml ROR1-strep). .. L11 clone supernatants were pre-diluted 3-fold in ELISA buffer (PBS-T containing 1% BSA), while supernatants from transiently transfected 293T cells were pre-diluted 50-fold.

    Binding Assay:

    Article Title: The Cryptosporidium parvum C-Type Lectin CpClec Mediates Infection of Intestinal Epithelial Cells via Interactions with Sulfated Proteoglycans
    Article Snippet: .. To control for a possible effect of calcium on the binding of biotinylated anti-mouse Fc IgG to Fc-tagged proteins, 10 μg/ml mouse IgG (Sigma) diluted in 0.05 M sodium carbonate-bicarbonate buffer, pH 9.6, was allowed to adsorb to Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C. .. Wells were washed three times with TBS, and nonspecific binding was blocked with TBS containing 5% NGS for 2 h at RT.

    Article Title: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with a mix of 2 µg mL−1 of streptavidin. .. After removing the blocking buffer, the wells were washed four times with PT with an automatized plate washer, and 1.2 equivalents (per total biotin binding sites) of biotinylated targets in 50 µL of PT buffer per well were added and incubated for 15 min at room temperature.

    Mouse Assay:

    Article Title: Novel Antibody Drug Conjugates Targeting Tumor-Associated Receptor Tyrosine Kinase ROR2 by Functional Screening of Fully Human Antibody Libraries Using Transpo-mAb Display on Progenitor B Cells
    Article Snippet: ELISA For determination of serum titers and antigen-binding, Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated with 2 μg/ml antigen diluted in coating buffer (100 mM bicarbonate/carbonate buffer). .. Plates were then washed twice with 0.05% Tween-20 in PBS (PBS-T), blocked at 37°C using PBS-T supplemented with 3% bovine serum albumin (BSA) (Carl Roth) for 1 h and washed again twice with PBS-T. Serum of the H2L2 and C57BL/6 mice was pre-diluted 1:100 in PBS-T/1% BSA.

    Phosphatase Assay:

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: After 22–26 h at 37 °C, 50 μl of medium was assayed for SEAP activity using a Phospha-Light TROPIX phosphatase assay (Applied Biosystems) in black assay plates and luminescence was measured after 45 min on a Thermo-Fisher Varioskan Flash Luminometer. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Isolation:

    Article Title: HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans
    Article Snippet: The apoE protein content from each isolated HDL sample was analyzed by enzyme-linked immunosorbent assay on a 96-well plate. .. Briefly, the Nunc Immuno-maxisorp 96-well plates (Nalge Nunc International, Rochester, NY) were coated with 10 μg/ml mouse anti-human apoE monoclonal antibody 6C5 in PBS and incubated overnight at 4°C.

    Next-Generation Sequencing:

    Article Title: The Cryptosporidium parvum C-Type Lectin CpClec Mediates Infection of Intestinal Epithelial Cells via Interactions with Sulfated Proteoglycans
    Article Snippet: Cells were washed three times with Tris-buffered saline (TBS; 50 mM Tris-HCl, 150 mM NaCl, pH 7.4), and nonspecific binding was blocked with TBS containing 2% NGS overnight at 4°C. .. To control for a possible effect of calcium on the binding of biotinylated anti-mouse Fc IgG to Fc-tagged proteins, 10 μg/ml mouse IgG (Sigma) diluted in 0.05 M sodium carbonate-bicarbonate buffer, pH 9.6, was allowed to adsorb to Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C.

    Purification:

    Article Title: Heterosubtypic T‐cell responses against avian influenza H5 haemagglutinin are frequently detected in individuals vaccinated against or previously infected with human subtypes of influenza
    Article Snippet: .. Nunc‐immuno MaxiSorp 96‐well plates (Thermo Fisher Scientific, Rochester, NY, USA) were coated with H1 (A/New Caledonia/20/99), H3 (A/Wyoming/3/2003) or H5 (A/Vietnam/1203/2004) purified ( > 90%) recombinant HA (rHA) (Protein Sciences, Meriden, CT, USA) diluted in carbonate buffer (pH 9·7) to 100 ng/ml. .. One hundred microlitres per well of this antigen solution was added to the plate, which was incubated overnight at 4°C.

    Avidin-Biotin Assay:

    Article Title: HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans
    Article Snippet: Briefly, the Nunc Immuno-maxisorp 96-well plates (Nalge Nunc International, Rochester, NY) were coated with 10 μg/ml mouse anti-human apoE monoclonal antibody 6C5 in PBS and incubated overnight at 4°C. .. The plate was then incubated for 1 hour with avidin alkaline phosphatase (Sigma-Aldrich).

    Blocking Assay:

    Article Title: Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (ThermoScientific) were coated overnight at 4 °C with 100 µL of a 30 nM SUMO-amphSH3 solution in NaHCO3 (50 mM, pH 9.6). .. Wells were then blocked with 200 µL of blocking buffer (PBS, 0.5% (wt/vol) biotin-free BSA) for 1 h at room temperature.

    Article Title: Proteomic Analysis of Sera from Individuals with Diffuse Cutaneous Systemic Sclerosis Reveals a Multianalyte Signature Associated with Clinical Improvement during Imatinib Mesylate Treatment
    Article Snippet: We coated Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) with goat anti-human capture antibodies or 10 mg/ml bovine serum albumin (BSA) in phosphate buffered saline (PBS) overnight at 4°C. .. After washing 5× in wash buffer (PBS with 0.05% Tween), plates were blocked with blocking buffer (10 mg/ml BSA in PBS with 0.05% Tween).

    Article Title: EsiB, a Novel Pathogenic Escherichia coli Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation
    Article Snippet: Briefly, Nunc-Immuno MaxiSorp 96-well plates (Thermo, Fisher Scientific) were coated overnight at 4°C with 0.5 µg of recombinant protein EsiB diluted in phosphate-buffered saline (PBS). .. After blocking with a 3% (wt/vol) polyvinylpyrrolidone (PVP) solution for 1 h at 37°C, plates were incubated with diluted patient sera for 2 h at 37°C.

    Article Title: Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
    Article Snippet: ELISAs were carried out in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 2 μg/ml of antigen in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C. .. Plates were washed with PBS-Tween and blocked with 10% Casein Block (Thermo Scientific).

    Article Title: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with a mix of 2 µg mL−1 of streptavidin. .. Wells were then blocked with 200 µL of blocking buffer (PBS, 0.5% (wt/vol) BSA) for 1 h at 37 °C.

    Article Title: Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
    Article Snippet: Cross-competition ELISAs were carried out in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 2 μg/ml of Pfs48/45-FL in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C. .. Plates were washed with PBS-Tween and blocked with 10% Casein Block (Thermo Scientific).

    Titration:

    Article Title: Elevated IgG Responses in Infants Are Associated With Reduced Prevalence of Mycobacterium tuberculosis Infection
    Article Snippet: Briefly, Nunc Immuno Maxisorp 96-well plates (Thermo Fisher Scientific) were coated with antibody or antigen and incubated at 37°C for 3 h, following which plates were blocked overnight at 4°C. .. Arbitrary antibody responses were determined from sample titration curves (optical density vs. sample dilution) as previously described , enabling a relative assessment of antibody levels in serum.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Novel Antibody Drug Conjugates Targeting Tumor-Associated Receptor Tyrosine Kinase ROR2 by Functional Screening of Fully Human Antibody Libraries Using Transpo-mAb Display on Progenitor B Cells
    Article Snippet: .. ELISA For determination of serum titers and antigen-binding, Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated with 2 μg/ml antigen diluted in coating buffer (100 mM bicarbonate/carbonate buffer). .. For determination of IgG titers, plates were coated with 2 μg/ml AffiniPure donkey anti-human IgG F(ab′)2 fragment, Fcγ-specific (109-006-008) or AffiniPure goat anti-human IgG F(ab′)2 fragment, Fcγ-specific (109-006-098, both Jackson Immunoresearch) diluted in coating buffer overnight at 4°C.

    Article Title: Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis
    Article Snippet: Paragraph title: ELISA assay ... Nunc-Immuno MaxiSorp 96-well plates (ThermoScientific) were coated overnight at 4 °C with 100 µL of a 30 nM SUMO-amphSH3 solution in NaHCO3 (50 mM, pH 9.6).

    Article Title: Transpo-mAb display: Transposition-mediated B cell display and functional screening of full-length IgG antibody libraries
    Article Snippet: Paragraph title: ELISA ... For determination of antigen-binding, Nunc-Immuno MaxiSorp 96-well plates (Thermo Scientific) were coated with antigens diluted in coating buffer (100 mM bicarbonate/carbonate buffer), using previously determined limiting concentrations of antigen to minimize avidity effects (0.25 μg/ml Meso-strep, 0.125 μg/ml ROR1-strep).

    Article Title: HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans
    Article Snippet: Paragraph title: Quantification of apoE in HDL Samples using Enzyme-Linked Immunosorbent Assay ... Briefly, the Nunc Immuno-maxisorp 96-well plates (Nalge Nunc International, Rochester, NY) were coated with 10 μg/ml mouse anti-human apoE monoclonal antibody 6C5 in PBS and incubated overnight at 4°C.

    Article Title: Heterosubtypic T‐cell responses against avian influenza H5 haemagglutinin are frequently detected in individuals vaccinated against or previously infected with human subtypes of influenza
    Article Snippet: Paragraph title: HA ELISA ... Nunc‐immuno MaxiSorp 96‐well plates (Thermo Fisher Scientific, Rochester, NY, USA) were coated with H1 (A/New Caledonia/20/99), H3 (A/Wyoming/3/2003) or H5 (A/Vietnam/1203/2004) purified ( > 90%) recombinant HA (rHA) (Protein Sciences, Meriden, CT, USA) diluted in carbonate buffer (pH 9·7) to 100 ng/ml.

    Article Title: Proteomic Analysis of Sera from Individuals with Diffuse Cutaneous Systemic Sclerosis Reveals a Multianalyte Signature Associated with Clinical Improvement during Imatinib Mesylate Treatment
    Article Snippet: Paragraph title: Chemokine ELISA ... We coated Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) with goat anti-human capture antibodies or 10 mg/ml bovine serum albumin (BSA) in phosphate buffered saline (PBS) overnight at 4°C.

    Article Title: EsiB, a Novel Pathogenic Escherichia coli Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation
    Article Snippet: Anti-EsiB immunoglobulin G (IgG) titers of patient sera were measured by enzyme-linked immunosorbent assay (ELISA). .. Briefly, Nunc-Immuno MaxiSorp 96-well plates (Thermo, Fisher Scientific) were coated overnight at 4°C with 0.5 µg of recombinant protein EsiB diluted in phosphate-buffered saline (PBS).

    Article Title: Elevated IgG Responses in Infants Are Associated With Reduced Prevalence of Mycobacterium tuberculosis Infection
    Article Snippet: Paragraph title: Non-Specific and Antigen-Specific ELISA ... Briefly, Nunc Immuno Maxisorp 96-well plates (Thermo Fisher Scientific) were coated with antibody or antigen and incubated at 37°C for 3 h, following which plates were blocked overnight at 4°C.

    Article Title: The Cryptosporidium parvum C-Type Lectin CpClec Mediates Infection of Intestinal Epithelial Cells via Interactions with Sulfated Proteoglycans
    Article Snippet: To assess whether Ca2+ could reverse inhibition by EGTA, 200 nM CpClec-Fc or Fc alone was incubated with 5 mM EGTA for 30 min at RT; this was followed by incubation with increasing concentrations of CaCl2 for an additional 10 min at RT, and binding was measured by ELISA. .. To control for a possible effect of calcium on the binding of biotinylated anti-mouse Fc IgG to Fc-tagged proteins, 10 μg/ml mouse IgG (Sigma) diluted in 0.05 M sodium carbonate-bicarbonate buffer, pH 9.6, was allowed to adsorb to Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C.

    Article Title: Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
    Article Snippet: Paragraph title: Enzyme-linked immunosorbent assay (ELISA) ... ELISAs were carried out in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 2 μg/ml of antigen in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Article Title: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with a mix of 2 µg mL−1 of streptavidin. .. Thereafter, wells were washed four times with PT buffer and 100 µL of rescued phage supernatant was added to the ELISA plates and incubated on a rocking shaker for 1 h. Following eight washes with PT, 100 µL of horseradish-peroxidase-conjugated anti-M13 monoclonal antibody (GE Healthcare, diluted at 1:5000 in PBT) was added to each well.

    Article Title: Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus
    Article Snippet: Paragraph title: Indirect BAFF ELISA. ... We coated Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) overnight at 4°C with BAFF (R & D) at 1 μg/ml diluted in carbonate buffer (pH 9.5).

    Concentration Assay:

    Article Title: Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
    Article Snippet: ELISAs were carried out in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 2 μg/ml of antigen in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C. .. Hybridoma supernatants were added to the wells undiluted, while mAbs were diluted to a starting concentration of 5 µg/ml and sera were diluted to a starting concentration between 1:100 and 1:3000.

    Article Title: Structural basis for recognition of the malaria vaccine candidate Pfs48/45 by a transmission blocking antibody
    Article Snippet: Cross-competition ELISAs were carried out in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 2 μg/ml of Pfs48/45-FL in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C. .. Fifteen minutes later, biotinylated antibody was added to the wells at a concentration of 2.5 µg/ml.

    Incubation:

    Article Title: Functional recruitment of dynamin requires multimeric interactions for efficient endocytosis
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (ThermoScientific) were coated overnight at 4 °C with 100 µL of a 30 nM SUMO-amphSH3 solution in NaHCO3 (50 mM, pH 9.6). .. Biotinylated peptide dilutions were performed in PT buffer and 100 µL of each dilution was added to the ELISA plates and incubated on a rocking shaker for 1 h at room temperature.

    Article Title: HDL-ApoE Content Regulates the Displacement of Hepatic Lipase from Cell Surface Proteoglycans
    Article Snippet: .. Briefly, the Nunc Immuno-maxisorp 96-well plates (Nalge Nunc International, Rochester, NY) were coated with 10 μg/ml mouse anti-human apoE monoclonal antibody 6C5 in PBS and incubated overnight at 4°C. ..

    Article Title: Heterosubtypic T‐cell responses against avian influenza H5 haemagglutinin are frequently detected in individuals vaccinated against or previously infected with human subtypes of influenza
    Article Snippet: Nunc‐immuno MaxiSorp 96‐well plates (Thermo Fisher Scientific, Rochester, NY, USA) were coated with H1 (A/New Caledonia/20/99), H3 (A/Wyoming/3/2003) or H5 (A/Vietnam/1203/2004) purified ( > 90%) recombinant HA (rHA) (Protein Sciences, Meriden, CT, USA) diluted in carbonate buffer (pH 9·7) to 100 ng/ml. .. One hundred microlitres per well of this antigen solution was added to the plate, which was incubated overnight at 4°C.

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: Heat-inactivated test sera were diluted fourfold from 1:9 to 1:2,304 in 10% fetal bovine sera (FBS) in DMEM and incubated 1:1 with ChAd63 expressing the secreted alkaline phosphatase (SEAP) gene (8 × 107 vp ml−1 ) for 1 h at 37 °C. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Article Title: EsiB, a Novel Pathogenic Escherichia coli Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation
    Article Snippet: Briefly, Nunc-Immuno MaxiSorp 96-well plates (Thermo, Fisher Scientific) were coated overnight at 4°C with 0.5 µg of recombinant protein EsiB diluted in phosphate-buffered saline (PBS). .. After blocking with a 3% (wt/vol) polyvinylpyrrolidone (PVP) solution for 1 h at 37°C, plates were incubated with diluted patient sera for 2 h at 37°C.

    Article Title: Elevated IgG Responses in Infants Are Associated With Reduced Prevalence of Mycobacterium tuberculosis Infection
    Article Snippet: .. Briefly, Nunc Immuno Maxisorp 96-well plates (Thermo Fisher Scientific) were coated with antibody or antigen and incubated at 37°C for 3 h, following which plates were blocked overnight at 4°C. .. Secondary antibody was added (50 μl/well) after which the reactions were developed using PNP substrate (Sigma Aldrich).

    Article Title: The Cryptosporidium parvum C-Type Lectin CpClec Mediates Infection of Intestinal Epithelial Cells via Interactions with Sulfated Proteoglycans
    Article Snippet: To assess whether Ca2+ could reverse inhibition by EGTA, 200 nM CpClec-Fc or Fc alone was incubated with 5 mM EGTA for 30 min at RT; this was followed by incubation with increasing concentrations of CaCl2 for an additional 10 min at RT, and binding was measured by ELISA. .. To control for a possible effect of calcium on the binding of biotinylated anti-mouse Fc IgG to Fc-tagged proteins, 10 μg/ml mouse IgG (Sigma) diluted in 0.05 M sodium carbonate-bicarbonate buffer, pH 9.6, was allowed to adsorb to Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C.

    Article Title: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
    Article Snippet: Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with a mix of 2 µg mL−1 of streptavidin. .. After removing the blocking buffer, the wells were washed four times with PT with an automatized plate washer, and 1.2 equivalents (per total biotin binding sites) of biotinylated targets in 50 µL of PT buffer per well were added and incubated for 15 min at room temperature.

    Inhibition:

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: Anti-vector neutralization titres were defined as the dilution of serum showing 50% reduction in SEAP activity, based on observed % inhibition values relative to SEAP activity from virus alone. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Article Title: The Cryptosporidium parvum C-Type Lectin CpClec Mediates Infection of Intestinal Epithelial Cells via Interactions with Sulfated Proteoglycans
    Article Snippet: To assess whether Ca2+ could reverse inhibition by EGTA, 200 nM CpClec-Fc or Fc alone was incubated with 5 mM EGTA for 30 min at RT; this was followed by incubation with increasing concentrations of CaCl2 for an additional 10 min at RT, and binding was measured by ELISA. .. To control for a possible effect of calcium on the binding of biotinylated anti-mouse Fc IgG to Fc-tagged proteins, 10 μg/ml mouse IgG (Sigma) diluted in 0.05 M sodium carbonate-bicarbonate buffer, pH 9.6, was allowed to adsorb to Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C.

    Activity Assay:

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: Anti-vector neutralization titres were defined as the dilution of serum showing 50% reduction in SEAP activity, based on observed % inhibition values relative to SEAP activity from virus alone. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Cell Culture:

    Article Title: Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules
    Article Snippet: The individual colonies were cultured overnight at 37 °C while shaking in a microplate deep well (Porvair Sciences) containing 450 µL of 2x YT medium, 50 µg mL−1 carbenicillin and 1010 M13KO7 helper phage per well. .. Nunc-Immuno MaxiSorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with a mix of 2 µg mL−1 of streptavidin.

    Expressing:

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: Heat-inactivated test sera were diluted fourfold from 1:9 to 1:2,304 in 10% fetal bovine sera (FBS) in DMEM and incubated 1:1 with ChAd63 expressing the secreted alkaline phosphatase (SEAP) gene (8 × 107 vp ml−1 ) for 1 h at 37 °C. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Neutralization:

    Article Title: Protective CD8+ T-cell immunity to human malaria induced by chimpanzee adenovirus-MVA immunisation
    Article Snippet: Anti-vector neutralization titres were defined as the dilution of serum showing 50% reduction in SEAP activity, based on observed % inhibition values relative to SEAP activity from virus alone. .. Anti-TRAP IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg ml−1 of TRAP protein in carbonate–bicarbonate coating buffer (Sigma) overnight at 4 °C.

    Staining:

    Article Title: A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis: First-in-human trial of ChAd63-KH
    Article Snippet: Acquisition was performed on the day of staining on a CyAn ADP (Beckman Coulter) and at least 500,000 events were collected per sample. .. Anti-KH IgG ELISAs were performed in Nunc-Immuno Maxisorp 96-well plates (Thermo Scientific) coated with 1 μg/ml of KH protein in carbonate-bicarbonate coating buffer (Sigma) overnight at 4 o C. Plates were washed with PBS Tween and blocked with 1% BSA.

    Centrifugation:

    Article Title: Heterosubtypic T‐cell responses against avian influenza H5 haemagglutinin are frequently detected in individuals vaccinated against or previously infected with human subtypes of influenza
    Article Snippet: HA ELISA Sera from participants were obtained by centrifugation of SSTTM Vacutainer tubes (BD, North Ryde, NSW, Australia) containing 4–8·5 ml blood at 1110 g . .. Nunc‐immuno MaxiSorp 96‐well plates (Thermo Fisher Scientific, Rochester, NY, USA) were coated with H1 (A/New Caledonia/20/99), H3 (A/Wyoming/3/2003) or H5 (A/Vietnam/1203/2004) purified ( > 90%) recombinant HA (rHA) (Protein Sciences, Meriden, CT, USA) diluted in carbonate buffer (pH 9·7) to 100 ng/ml.

    Recombinant:

    Article Title: Heterosubtypic T‐cell responses against avian influenza H5 haemagglutinin are frequently detected in individuals vaccinated against or previously infected with human subtypes of influenza
    Article Snippet: .. Nunc‐immuno MaxiSorp 96‐well plates (Thermo Fisher Scientific, Rochester, NY, USA) were coated with H1 (A/New Caledonia/20/99), H3 (A/Wyoming/3/2003) or H5 (A/Vietnam/1203/2004) purified ( > 90%) recombinant HA (rHA) (Protein Sciences, Meriden, CT, USA) diluted in carbonate buffer (pH 9·7) to 100 ng/ml. .. One hundred microlitres per well of this antigen solution was added to the plate, which was incubated overnight at 4°C.

    Article Title: EsiB, a Novel Pathogenic Escherichia coli Secretory Immunoglobulin A-Binding Protein Impairing Neutrophil Activation
    Article Snippet: .. Briefly, Nunc-Immuno MaxiSorp 96-well plates (Thermo, Fisher Scientific) were coated overnight at 4°C with 0.5 µg of recombinant protein EsiB diluted in phosphate-buffered saline (PBS). .. After blocking with a 3% (wt/vol) polyvinylpyrrolidone (PVP) solution for 1 h at 37°C, plates were incubated with diluted patient sera for 2 h at 37°C.

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  • 93
    Thermo Fisher nunc immuno maxisorp 96 well plates
    Nunc Immuno Maxisorp 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc immuno maxisorp 96 well plates/product/Thermo Fisher
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nunc immuno maxisorp 96 well plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher nunc immuno maxisorp 96 well microtiter plates
    Nunc Immuno Maxisorp 96 Well Microtiter Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc immuno maxisorp 96 well microtiter plates/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nunc immuno maxisorp 96 well microtiter plates - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

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