nunc fluoronunc luminunc 96 well plates  (Thermo Fisher)


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    Name:
    Nunc FluoroNunc LumiNunc 96 Well Plates
    Description:
    Use Thermo Scientific Nunc FluoroNunc LumiNunc 96 Well Solid plates for quantitative and qualitative solid phase immunoassays e g FLISA and binding assays Thermo Scientific Nunc Immuno plates offer lot to lot reliability well to well reproducibility and are backed by industry leading quality standards and certifications
    Catalog Number:
    437796
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    Clinical|Diagnostic Development
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    Structured Review

    Thermo Fisher nunc fluoronunc luminunc 96 well plates
    Use Thermo Scientific Nunc FluoroNunc LumiNunc 96 Well Solid plates for quantitative and qualitative solid phase immunoassays e g FLISA and binding assays Thermo Scientific Nunc Immuno plates offer lot to lot reliability well to well reproducibility and are backed by industry leading quality standards and certifications
    https://www.bioz.com/result/nunc fluoronunc luminunc 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nunc fluoronunc luminunc 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

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    Centrifugation:

    Article Title: Investigations on Collectin Liver 1 *
    Article Snippet: FluoroNunc MaxiSorp (Thermo Scientific, 437958) microtiter wells were each coated with 0.5 μg of 187-10 anti-CL-L1 or with 0.5 μg of normal rabbit IgG (Dako) in 100 μl of PBS, overnight at room temperature (RT). .. The heat-aggregated hIgG was prepared by incubation of hIgG (Beriglobin) at 1 mg/ml PBS at 60 °C for 30 min followed by centrifugation at 3000 × g for 10 min to remove large aggregates.

    Luciferase:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: The Bright-Glo™ Luciferase Assay System and CellTiter-Glo® Luminescent Cell Viability Assay were purchased from Promega (Madison, WI). .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Stable Transfection:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: The mink lung epithelial (Mv1Lu) cell line stably transfected with SBE (CAGA)12 -Luc reporter gene was a generous gift from professor F. M. Hoffmann (University of Wisconsin-Madison). .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Synthesized:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: Peptides were either purchased from Biomatik (Wilmington, DE) or synthesized at the Peptide Synthesis Facility at the University of Wisconsin-Madison and purified by HPLC to > 80% purity. .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Immunostaining:

    Article Title: Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis
    Article Snippet: Renal inflammation was evaluated by standard immunostaining with antibodies against macrophages (anti-macrophage CD163), B-T lymphocytes (CD45), and CD3 T-lymphocytes. .. Systemic inflammation was assessed by IL-1β levels, quantified by ELISA for porcine IL-1β developed using Nunc MaxSorp plate (ThermoFisher Scientific, 437796) coated with 100μl of IL-1β capture antibody 2.0 μg/ml (R & D systems, DY681) and 100μl of pig plasma samples.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance
    Article Snippet: Plasma OSTN concentrations were measured by sandwich chemiluminescence enzyme immunoassay (CLEIA), modifying the previously reported protocol for mouse ANP CLEIA ( ). .. The 96-well white plates (437796, Nunc C96 White MaxiSorp Immunoplate; Thermo Fisher Scientific) were coated with anti-mMUS1G2 (0.5 μg/well) in carbonate-bicarbonate buffer (pH 9.6) at room temperature overnight.

    Article Title: Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis
    Article Snippet: .. Systemic inflammation was assessed by IL-1β levels, quantified by ELISA for porcine IL-1β developed using Nunc MaxSorp plate (ThermoFisher Scientific, 437796) coated with 100μl of IL-1β capture antibody 2.0 μg/ml (R & D systems, DY681) and 100μl of pig plasma samples. .. The plate was read by the SynergyMx plate reader (BioTek), set at standard luminescence reading mode, for 1 hour at 10-min intervals.

    Article Title: Characterization of the Antigenic Heterogeneity of Lipoarabinomannan, the Major Surface Glycolipid of Mycobacterium tuberculosis, and Complexity of Antibody Specificities toward This Antigen
    Article Snippet: AP-streptavidin was used at 1:1,000 dilution and developed with DynaLight Substrate with RapidGlow Enhancer (catalog number 4475406; Thermo Fisher Scientific) diluted 1:10 in DEA buffer (pH 9.8), whereas HRP-streptavidin was used at a 1:15,000 dilution and developed with SuperSignal ELISA Femto Substrate (catalog number 37075; Thermo Fisher Scientific). .. Assays were run in white LumiNunc 96-Well Plates (catalog number 437796; Thermo Fisher Scientific) with 2% BSA blocking agent.

    Incubation:

    Article Title: Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance
    Article Snippet: The 96-well white plates (437796, Nunc C96 White MaxiSorp Immunoplate; Thermo Fisher Scientific) were coated with anti-mMUS1G2 (0.5 μg/well) in carbonate-bicarbonate buffer (pH 9.6) at room temperature overnight. .. On the next day, after additional incubation at room temperature for 1 hour, plates were washed and incubated with MAB26260 at room temperature for 1 hour.

    Article Title: Targeted Delivery of siRNA to Transferrin Receptor Overexpressing Tumor Cells via Peptide Modified Polyethylenimine
    Article Snippet: Polyplexes were prepared with bPEI or HAI-SPDP-bPEI and 50 pmol of siRNA at different N/P ratios (1, 2, 3, 5, 7, 10) in 100 µL per well in a FluoroNunc 96 well white plate (Thermo Fisher). .. Subsequently, 30 µL of 4 × SYBR Gold was added to each well and incubated for 10 min in the dark.

    Article Title: Investigations on Collectin Liver 1 *
    Article Snippet: FluoroNunc MaxiSorp (Thermo Scientific, 437958) microtiter wells were each coated with 0.5 μg of 187-10 anti-CL-L1 or with 0.5 μg of normal rabbit IgG (Dako) in 100 μl of PBS, overnight at room temperature (RT). .. Wells were blocked by incubation with TBS/Tw for 1 h, and subsequently washed with TBS/Tw.

    Article Title: Characterization of the Antigenic Heterogeneity of Lipoarabinomannan, the Major Surface Glycolipid of Mycobacterium tuberculosis, and Complexity of Antibody Specificities toward This Antigen
    Article Snippet: Assays were run in white LumiNunc 96-Well Plates (catalog number 437796; Thermo Fisher Scientific) with 2% BSA blocking agent. .. The AP plates were incubated after addition of substrate for 6–10 min at room temperature in the dark, whereas the HRP plates were read after incubation for 1 min in the dark.

    Activity Assay:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Paragraph title: Inhibition of C2 activity ... A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Expressing:

    Article Title: Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis
    Article Snippet: In addition, renal expression of TNF-α (Santa Cruz 1:200) was quantified by Western Blot , . .. Systemic inflammation was assessed by IL-1β levels, quantified by ELISA for porcine IL-1β developed using Nunc MaxSorp plate (ThermoFisher Scientific, 437796) coated with 100μl of IL-1β capture antibody 2.0 μg/ml (R & D systems, DY681) and 100μl of pig plasma samples.

    Western Blot:

    Article Title: Adipose tissue-derived mesenchymal stem cells improve revascularization outcomes to restore renal function in swine atherosclerotic renal artery stenosis
    Article Snippet: In addition, renal expression of TNF-α (Santa Cruz 1:200) was quantified by Western Blot , . .. Systemic inflammation was assessed by IL-1β levels, quantified by ELISA for porcine IL-1β developed using Nunc MaxSorp plate (ThermoFisher Scientific, 437796) coated with 100μl of IL-1β capture antibody 2.0 μg/ml (R & D systems, DY681) and 100μl of pig plasma samples.

    High Performance Liquid Chromatography:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: Peptides were either purchased from Biomatik (Wilmington, DE) or synthesized at the Peptide Synthesis Facility at the University of Wisconsin-Madison and purified by HPLC to > 80% purity. .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Transfection:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: The mink lung epithelial (Mv1Lu) cell line stably transfected with SBE (CAGA)12 -Luc reporter gene was a generous gift from professor F. M. Hoffmann (University of Wisconsin-Madison). .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Purification:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Inhibition of C2 activity The in-vitro inhibition of C2 activity by purified IgGs from mice and cattle was assessed using assays for the hydrolysis of a fluorogenic peptide substrate. .. A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: 45 μl of t-SNARE liposomes were reacted with 5 μl of v-SNARE liposomes in a total of 75 μl of assay buffer (25 m m HEPES-KOH, pH 7.8, 100 m m KCl, 10% glycerol, 1 m m DTT, 0.2 m m EGTA) in white 96-well FluoroNunc plates (Thermo Fisher, Waltham, MA). .. Purified synaptotagmin 1 (10 μ m ) was added to the buffer along with a concentration-response curve of purified bovine Gβ1 γ1 followed by the addition of the t-SNARE liposomes.

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: Peptides were either purchased from Biomatik (Wilmington, DE) or synthesized at the Peptide Synthesis Facility at the University of Wisconsin-Madison and purified by HPLC to > 80% purity. .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Inhibition:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Paragraph title: Inhibition of C2 activity ... A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Sequencing:

    Article Title: Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance
    Article Snippet: This antibody specifically recognizes the C-terminal sequence of mouse OSTN, showing only 0.2% affinity to human OSTN and no cross-reactivity with ANP, BNP, or CNP at the 1/450,000 dilution. .. The 96-well white plates (437796, Nunc C96 White MaxiSorp Immunoplate; Thermo Fisher Scientific) were coated with anti-mMUS1G2 (0.5 μg/well) in carbonate-bicarbonate buffer (pH 9.6) at room temperature overnight.

    Affinity Purification:

    Article Title: Investigations on Collectin Liver 1 *
    Article Snippet: Paragraph title: Analytical Affinity Purification of CL-L1 ... FluoroNunc MaxiSorp (Thermo Scientific, 437958) microtiter wells were each coated with 0.5 μg of 187-10 anti-CL-L1 or with 0.5 μg of normal rabbit IgG (Dako) in 100 μl of PBS, overnight at room temperature (RT).

    Binding Assay:

    Article Title: Investigations on Collectin Liver 1 *
    Article Snippet: FluoroNunc MaxiSorp (Thermo Scientific, 437958) microtiter wells were each coated with 0.5 μg of 187-10 anti-CL-L1 or with 0.5 μg of normal rabbit IgG (Dako) in 100 μl of PBS, overnight at room temperature (RT). .. Twelve anti-CL-L1 wells and 12 control wells each received 100 μl of EDTA plasma diluted with an equal volume of “binding buffer” (TBS/Tw with 10 m m EDTA, 100 μg of heat-aggregated hIgG, and 10 μg of normal rabbit IgG (Dako) per ml).

    Fluorescence:

    Article Title: Differential chemokine expression under the control of peripheral blood mononuclear cells issued from Alzheimer’s patients in a human blood brain barrier model
    Article Snippet: .. The flux of FD4 diluted in Hank's Balanced Salt Solution (HBSS) at 50 mg/1,5mL (0.4 g/L KCl, 0.06 g/L KH2 PO4 , 8 g/L NaCl, 0.35g/L NaHCO3 , 0.048 g/L Na2 HPO4 , 1 g/L D-Glucose, 0.14 g/L CaCl2 , 0.1g/L MgCl2 , 6H2 O, 0.1g/L MgSO4 , 7H2 O) through cellular layers was determined after taking samples (50 μL) from the upper and lower chambers after 0 and 60 min, and each sample was directly transferred into Nunc FluoroNunc/LumiNunc 96-Well plates, then fluorescence was measured by using a Varioskan Flash® microplate reader (Fisher ThermoScientific) with excitation at 485 nm and emission at 515 nm. .. This BBB permeability assay was performed under two conditions: insert without cells, i.e. only pre-coated insert (control) and BBB model without PBMCs.

    Article Title: Targeted Delivery of siRNA to Transferrin Receptor Overexpressing Tumor Cells via Peptide Modified Polyethylenimine
    Article Snippet: When siRNA is condensed by polymer and becomes inaccessible for SYBR Gold, the fluorescence will decrease. .. Polyplexes were prepared with bPEI or HAI-SPDP-bPEI and 50 pmol of siRNA at different N/P ratios (1, 2, 3, 5, 7, 10) in 100 µL per well in a FluoroNunc 96 well white plate (Thermo Fisher).

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C. .. Fluorescence was measured using a spectrofluorometer (Fluostar Optima, BMG Labtech GmbH, Offenburg, Germany) using excitation at 360 nm and emission at 460 nm.

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: 45 μl of t-SNARE liposomes were reacted with 5 μl of v-SNARE liposomes in a total of 75 μl of assay buffer (25 m m HEPES-KOH, pH 7.8, 100 m m KCl, 10% glycerol, 1 m m DTT, 0.2 m m EGTA) in white 96-well FluoroNunc plates (Thermo Fisher, Waltham, MA). .. All components of the fusion reaction, except v-SNARE vesicles, were combined and pre-warmed to 37 C for 15 min. NBD fluore s cence (excitation 460 nm/emission 538 nm) was continuously monitored over 80 min in a BioTek Synergy plate reader under continuous agitation, with fluorescence intensity being read every 8 s. After 20 min, CaCl2 was added to a final concentration of 1.2 m m in the assay with 0.2 m m being chelated by EGTA to yield an effective concentration of 1.0 m m .

    Aqueous Normal-phase Chromatography:

    Article Title: Circulating osteocrin stimulates bone growth by limiting C-type natriuretic peptide clearance
    Article Snippet: This antibody specifically recognizes the C-terminal sequence of mouse OSTN, showing only 0.2% affinity to human OSTN and no cross-reactivity with ANP, BNP, or CNP at the 1/450,000 dilution. .. The 96-well white plates (437796, Nunc C96 White MaxiSorp Immunoplate; Thermo Fisher Scientific) were coated with anti-mMUS1G2 (0.5 μg/well) in carbonate-bicarbonate buffer (pH 9.6) at room temperature overnight.

    Mouse Assay:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Inhibition of C2 activity The in-vitro inhibition of C2 activity by purified IgGs from mice and cattle was assessed using assays for the hydrolysis of a fluorogenic peptide substrate. .. A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Blocking Assay:

    Article Title: Characterization of the Antigenic Heterogeneity of Lipoarabinomannan, the Major Surface Glycolipid of Mycobacterium tuberculosis, and Complexity of Antibody Specificities toward This Antigen
    Article Snippet: .. Assays were run in white LumiNunc 96-Well Plates (catalog number 437796; Thermo Fisher Scientific) with 2% BSA blocking agent. .. The AP plates were incubated after addition of substrate for 6–10 min at room temperature in the dark, whereas the HRP plates were read after incubation for 1 min in the dark.

    Titration:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Ten ng of active C2 (determined by active site titration with E-64) [ ] diluted in 75 μl of 0.1% (v/v) Brij-35 was mixed with 75 μl of the different IgG solutions diluted in C2 assay buffer (100 mM Bis-Tris buffer, pH 6.4, 4 mM Na2 EDTA and 0.02% (w/v) NaN3 ) without dithiothreitol (DTT). .. A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Recombinant:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific. .. Recombinant human BMPR-IA (Gln24-Arg152) Fc chimera, recombinant human ActRII (Ser25-Pro134) Fc chimera, recombinant human endoglin-ED (residues 26-586) and recombinant human β-glycan-ED (residues 21-781) were purchased from R & D Systems (Minneapolis, MN).

    In Vitro:

    Article Title: Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    Article Snippet: Inhibition of C2 activity The in-vitro inhibition of C2 activity by purified IgGs from mice and cattle was assessed using assays for the hydrolysis of a fluorogenic peptide substrate. .. A 50 μl aliquot from the C2-IgG mixture was added per well of Fluoronunc Maxisorp 96-well plates (eBioScience, San Diego, CA, USA), followed by addition of 25 μl of assay buffer containing 8 mM DTT and activated for 1 min at 37°C.

    Laser Capture Microdissection:

    Article Title: Characterization of the Antigenic Heterogeneity of Lipoarabinomannan, the Major Surface Glycolipid of Mycobacterium tuberculosis, and Complexity of Antibody Specificities toward This Antigen
    Article Snippet: LAM-capture assays were performed using two sensitive ECL detection methods to enhance the signals. .. Assays were run in white LumiNunc 96-Well Plates (catalog number 437796; Thermo Fisher Scientific) with 2% BSA blocking agent.

    Concentration Assay:

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: 45 μl of t-SNARE liposomes were reacted with 5 μl of v-SNARE liposomes in a total of 75 μl of assay buffer (25 m m HEPES-KOH, pH 7.8, 100 m m KCl, 10% glycerol, 1 m m DTT, 0.2 m m EGTA) in white 96-well FluoroNunc plates (Thermo Fisher, Waltham, MA). .. Purified synaptotagmin 1 (10 μ m ) was added to the buffer along with a concentration-response curve of purified bovine Gβ1 γ1 followed by the addition of the t-SNARE liposomes.

    Staining:

    Article Title: Targeted Delivery of siRNA to Transferrin Receptor Overexpressing Tumor Cells via Peptide Modified Polyethylenimine
    Article Snippet: 4.6. siRNA Condensation Measured by a SYBR Gold Assay SYBR Gold is a fluorescent dye which is used to stain nucleic acids. .. Polyplexes were prepared with bPEI or HAI-SPDP-bPEI and 50 pmol of siRNA at different N/P ratios (1, 2, 3, 5, 7, 10) in 100 µL per well in a FluoroNunc 96 well white plate (Thermo Fisher).

    Cell Viability Assay:

    Article Title: Peptide ligands that use a novel binding site to target both TGF-\u03b2 receptors
    Article Snippet: The Bright-Glo™ Luciferase Assay System and CellTiter-Glo® Luminescent Cell Viability Assay were purchased from Promega (Madison, WI). .. LumiNunc™ Plates were purchased from Nunc Thermo Fisher Scientific.

    Permeability:

    Article Title: Differential chemokine expression under the control of peripheral blood mononuclear cells issued from Alzheimer’s patients in a human blood brain barrier model
    Article Snippet: Paragraph title: Blood brain barrier permeability ... The flux of FD4 diluted in Hank's Balanced Salt Solution (HBSS) at 50 mg/1,5mL (0.4 g/L KCl, 0.06 g/L KH2 PO4 , 8 g/L NaCl, 0.35g/L NaHCO3 , 0.048 g/L Na2 HPO4 , 1 g/L D-Glucose, 0.14 g/L CaCl2 , 0.1g/L MgCl2 , 6H2 O, 0.1g/L MgSO4 , 7H2 O) through cellular layers was determined after taking samples (50 μL) from the upper and lower chambers after 0 and 60 min, and each sample was directly transferred into Nunc FluoroNunc/LumiNunc 96-Well plates, then fluorescence was measured by using a Varioskan Flash® microplate reader (Fisher ThermoScientific) with excitation at 485 nm and emission at 515 nm.

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  • 99
    Thermo Fisher nunc fluoronunc luminunc 96 well plates
    Nunc Fluoronunc Luminunc 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc fluoronunc luminunc 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nunc fluoronunc luminunc 96 well plates - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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