nunc edge 96 well plates  (Thermo Fisher)


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    Nunc Edge 96 Well Plate
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    Catalog Number:
    167314
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    Structured Review

    Thermo Fisher nunc edge 96 well plates

    https://www.bioz.com/result/nunc edge 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nunc edge 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Diagnostic Assay:

    Article Title: The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria
    Article Snippet: Susceptibility to GA antimicrobial effects tested by OASTS E . coli and S . aureus laboratory strains were grown overnight in Sensititre® cation-adjusted Mueller-Hinton broth with N -tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer (CAMHBT, TREK Diagnostic System, Thermo Fisher Scientific, Oakwood village, OH) at 37 °C. .. Growth was analysed in Nunc Edge™ 96-well plates (Thermo Fisher) by OASTS using the oCelloScope™ instrument (Philips BioCell A/S, Allerød, Denmark).

    Centrifugation:

    Article Title: Therapeutic surfactant-stripped frozen micelles
    Article Snippet: The supernatants were transferred to 200 μl DCM, shaken for 5 min for extraction to remove absorbance interference by VK1, followed by subsequent centrifugation at 2,000g for 5 min. Haemolysis percentages were determined by absorbance measurements of the water phase at 540 nm. .. SRBCs (Innovative Research, Inc. # IR1-020ND) containing 5 × 106 cells in 0.1 ml PBS were added to each well of a flat bottom 96-well ELISA microtiter plates (Thermo Scientific # 267312) and the plates were incubated overnight at 4 °C.

    Filtration:

    Article Title: Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies
    Article Snippet: Paragraph title: Analytical gel filtration and activity assay ... TYR enzyme activity assays were performed at 298 K using 3,4-dihydroxyphenylalanine (L-DOPA) as substrate in a Nunc™ Edge 96-Well plate (Thermo Fisher Scientific) [ , ].

    Neutralization:

    Article Title: Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep
    Article Snippet: Serology Virus neutralization test was carried out as previously described [ ] with the exception that all sera were analysed both without (VNTw/d) and with (VNTd) heat-inactivation of the complement system (30 min at 56 °C) before testing. .. Briefly, successive two fold dilutions of 50 µL of sera, from 1/2 to 1/256 onwards, were made in 50 µL of Dulbecco’s Modified Eagles Medium (Gibco, Life Technologies, Ghent, Belgium) supplemented by 1000 IU penicillin/mL, 50 µg/mL gentamicin (Gibco, Life Technologies, Ghent, Belgium) and 250 µg/mL amphotericin B (Gibco, Life Technologies, Ghent, Belgium) (DMEM) in Nunc Edge 96-well plates (Thermo scientific, Waltham, MA, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Therapeutic surfactant-stripped frozen micelles
    Article Snippet: .. SRBCs (Innovative Research, Inc. # IR1-020ND) containing 5 × 106 cells in 0.1 ml PBS were added to each well of a flat bottom 96-well ELISA microtiter plates (Thermo Scientific # 267312) and the plates were incubated overnight at 4 °C. .. 20 μl of 1.8% glutaraldehyde (AMRESCO, # 0904C089) was gently added to wells containing SRBCs and the plate was incubated for 3 min at 25 °C.

    Incubation:

    Article Title: Therapeutic surfactant-stripped frozen micelles
    Article Snippet: .. SRBCs (Innovative Research, Inc. # IR1-020ND) containing 5 × 106 cells in 0.1 ml PBS were added to each well of a flat bottom 96-well ELISA microtiter plates (Thermo Scientific # 267312) and the plates were incubated overnight at 4 °C. .. 20 μl of 1.8% glutaraldehyde (AMRESCO, # 0904C089) was gently added to wells containing SRBCs and the plate was incubated for 3 min at 25 °C.

    Article Title: Geometric constraints of endothelial cell migration on electrospun fibres
    Article Snippet: Briefly, 10% (V/V) of PrestoBlue® reagent was added in each well (n = 3) and incubated at 37 °C for 2 hours. .. Three 100 µL media samples were transferred from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Article Title: Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep
    Article Snippet: Briefly, successive two fold dilutions of 50 µL of sera, from 1/2 to 1/256 onwards, were made in 50 µL of Dulbecco’s Modified Eagles Medium (Gibco, Life Technologies, Ghent, Belgium) supplemented by 1000 IU penicillin/mL, 50 µg/mL gentamicin (Gibco, Life Technologies, Ghent, Belgium) and 250 µg/mL amphotericin B (Gibco, Life Technologies, Ghent, Belgium) (DMEM) in Nunc Edge 96-well plates (Thermo scientific, Waltham, MA, USA). .. After four days of incubation, the titre was determined as the reciprocal of the highest serum dilution in which no lysis plaques could be identified in the cell monolayer under the light microscope.

    Article Title: Schwann cells promote endothelial cell migration
    Article Snippet: Briefly, 10% (v/v) of PrestoBlue® reagent was added in each well and the samples (n = 3) incubated at 37 ˚C for 2 hours. .. Three 100 µL media samples were taken from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Article Title: Tuwongella immobilis gen. nov., sp. nov., a novel non-motile bacterium within the phylum Planctomycetes
    Article Snippet: MBLW1T was grown for 48 h at 32 °C in Nunc Edge 96-well microplates (Thermo Scientific) and the optical density (λ=600 nm) was measured using a Tecan Infinite M200 microplate reader (Tecan). .. Salt tolerance of MBLW1T and G. obscuriglobus was compared in 24-well M1 agar plates containing increasing NaCl-concentrations [0.0 %–2.0 % (w/v)] after incubation for 5 days at 32 °C.

    Article Title: Assessment of Cr(VI)-Induced Cytotoxicity and Genotoxicity Using High Content Analysis
    Article Snippet: .. For assays in undifferentiated/proliferating Caco-2 cells, cells were seeded at a density of 1×104 cells/100 µl/well in Nunc Edge 96-well microplates (Thermo Fisher Scientific) 24 hr prior to incubation with compounds. .. For assays in differentiated Caco-2 monolayers, cells were seeded at a density of 2×104 cells/100 µl/well in Collagen I (BD Biosciences) coated Nunc Edge 96-well microplates (Thermo Fisher Scientific), and cultured for 21 days prior to incubation with compounds, with a media change every other day.

    Activity Assay:

    Article Title: Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies
    Article Snippet: .. TYR enzyme activity assays were performed at 298 K using 3,4-dihydroxyphenylalanine (L-DOPA) as substrate in a Nunc™ Edge 96-Well plate (Thermo Fisher Scientific) [ , ]. ..

    Modification:

    Article Title: Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep
    Article Snippet: .. Briefly, successive two fold dilutions of 50 µL of sera, from 1/2 to 1/256 onwards, were made in 50 µL of Dulbecco’s Modified Eagles Medium (Gibco, Life Technologies, Ghent, Belgium) supplemented by 1000 IU penicillin/mL, 50 µg/mL gentamicin (Gibco, Life Technologies, Ghent, Belgium) and 250 µg/mL amphotericin B (Gibco, Life Technologies, Ghent, Belgium) (DMEM) in Nunc Edge 96-well plates (Thermo scientific, Waltham, MA, USA). ..

    Flow Cytometry:

    Article Title: Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies
    Article Snippet: Elution from the gel filtration column was carried out at a flow rate of 0.5 mL/min, fractionation volume of 0.3 mL, and total running time of 40 min. .. TYR enzyme activity assays were performed at 298 K using 3,4-dihydroxyphenylalanine (L-DOPA) as substrate in a Nunc™ Edge 96-Well plate (Thermo Fisher Scientific) [ , ].

    Cell Culture:

    Article Title: Assessment of Cr(VI)-Induced Cytotoxicity and Genotoxicity Using High Content Analysis
    Article Snippet: Paragraph title: Cell Culture ... For assays in undifferentiated/proliferating Caco-2 cells, cells were seeded at a density of 1×104 cells/100 µl/well in Nunc Edge 96-well microplates (Thermo Fisher Scientific) 24 hr prior to incubation with compounds.

    Article Title: Quantitative Analysis of Mitochondrial Morphology and Membrane Potential in Living Cells Using High-Content Imaging, Machine Learning, and Morphological Binning
    Article Snippet: .. Cells of passages 15-25 were cultured to 80% confluency before trypsinization and seeded at a cell density of 8,000 cells per well in 96 well plates (Nunc Edge Plate, Thermo Scientific #167314) supplemented with 5% FCS (fetal calf serum) and edge reservoirs filled with phosphate-buffered saline (to prevent hydration-dependent microplate edge effects) (PBS; Life Technologies #14080-055). .. After cells reached 80% confluency (24 h), cells were washed in PBS, and media was changed to DMEM with 5.5 mM glucose without phenol red (Sigma-Aldrich #D-5030) and supplemented with 1% FCS to induce cell cycle arrest.

    Light Microscopy:

    Article Title: Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep
    Article Snippet: Briefly, successive two fold dilutions of 50 µL of sera, from 1/2 to 1/256 onwards, were made in 50 µL of Dulbecco’s Modified Eagles Medium (Gibco, Life Technologies, Ghent, Belgium) supplemented by 1000 IU penicillin/mL, 50 µg/mL gentamicin (Gibco, Life Technologies, Ghent, Belgium) and 250 µg/mL amphotericin B (Gibco, Life Technologies, Ghent, Belgium) (DMEM) in Nunc Edge 96-well plates (Thermo scientific, Waltham, MA, USA). .. After four days of incubation, the titre was determined as the reciprocal of the highest serum dilution in which no lysis plaques could be identified in the cell monolayer under the light microscope.

    Injection:

    Article Title: Therapeutic surfactant-stripped frozen micelles
    Article Snippet: SRBCs (Innovative Research, Inc. # IR1-020ND) containing 5 × 106 cells in 0.1 ml PBS were added to each well of a flat bottom 96-well ELISA microtiter plates (Thermo Scientific # 267312) and the plates were incubated overnight at 4 °C. .. Mice were injected intraperitoneally with 5 × 108 SRBCs in 0.5 ml saline.

    Fluorescence:

    Article Title: Geometric constraints of endothelial cell migration on electrospun fibres
    Article Snippet: Three 100 µL media samples were transferred from each well into a Nunc™ 96-well plate (Thermo Scientific, cat. .. Fluorescence was measured at 540–570 nm excitation 580–610 nm emission in VICTOR3 ™ 1420 Multilabel Counter (PerkinElmer).

    Article Title: Schwann cells promote endothelial cell migration
    Article Snippet: Three 100 µL media samples were taken from each well into a Nunc™ 96-well plate (Thermo Scientific, cat. .. Fluorescence was measured at 540–570 nm excitation 580–610 nm emission in VICTOR3 ™ 1420 Multilabel Counter (PerkinElmer).

    Article Title: The combined use of paclitaxel-loaded nanoparticles with a low-molecular-weight copolymer inhibitor of P-glycoprotein to overcome drug resistance
    Article Snippet: .. A small amount of the labeled copolymers was dissolved in DMF, and the TMRCA labeling efficiency of the diblock copolymers was determined by spectrofluorometry against a calibration curve of free TMRCA in DMF using a Synergy MX (Biotek, Winooski, VT, USA) fluorescence plate reader and a Nunc 96-well plate (Thermo Fisher Scientific) using an excitation wavelength of 548 nm and an emission wavelength of 572 nm. .. The fluorescent labeling efficiency for PCL19 and PCL104 was approximately 50.3% and 63.3%, respectively.

    Temperature Regulation:

    Article Title: The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria
    Article Snippet: Growth was analysed in Nunc Edge™ 96-well plates (Thermo Fisher) by OASTS using the oCelloScope™ instrument (Philips BioCell A/S, Allerød, Denmark). .. Each well was scanned repeatedly every 15 min for the first 2 h and every 10 min for the following 5 h. The OASTS instrument was placed inside an Innova 44 incubator (Eppendorf, Hamburg, Germany) for precise temperature regulation.

    Microscopy:

    Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis
    Article Snippet: Single cell growth kinetics Growth of Diaphanoeca grandis was also monitored at the single-cell level in the oCelloScope optical detection system (Biosense Solutions Aps, Denmark), which is an automatic digital microscope equipped with a 4x optical magnification (200x effective magnification) , . .. In the oCelloScope, D. grandis was grown in NUNCTM Edge 96-well plates (Thermo Scientific, USA) containing 200 μL of sterilised seawater (salinity = 10‰, temp.

    Mouse Assay:

    Article Title: Therapeutic surfactant-stripped frozen micelles
    Article Snippet: SRBCs (Innovative Research, Inc. # IR1-020ND) containing 5 × 106 cells in 0.1 ml PBS were added to each well of a flat bottom 96-well ELISA microtiter plates (Thermo Scientific # 267312) and the plates were incubated overnight at 4 °C. .. Mice were injected intraperitoneally with 5 × 108 SRBCs in 0.5 ml saline.

    Labeling:

    Article Title: The combined use of paclitaxel-loaded nanoparticles with a low-molecular-weight copolymer inhibitor of P-glycoprotein to overcome drug resistance
    Article Snippet: .. A small amount of the labeled copolymers was dissolved in DMF, and the TMRCA labeling efficiency of the diblock copolymers was determined by spectrofluorometry against a calibration curve of free TMRCA in DMF using a Synergy MX (Biotek, Winooski, VT, USA) fluorescence plate reader and a Nunc 96-well plate (Thermo Fisher Scientific) using an excitation wavelength of 548 nm and an emission wavelength of 572 nm. .. The fluorescent labeling efficiency for PCL19 and PCL104 was approximately 50.3% and 63.3%, respectively.

    Viability Assay:

    Article Title: Geometric constraints of endothelial cell migration on electrospun fibres
    Article Snippet: Paragraph title: Viability assay ... Three 100 µL media samples were transferred from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Article Title: Schwann cells promote endothelial cell migration
    Article Snippet: Paragraph title: Viability assay ... Three 100 µL media samples were taken from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Time-lapse Microscopy:

    Article Title: The Immunomodulatory Drug Glatiramer Acetate is Also an Effective Antimicrobial Agent that Kills Gram-negative Bacteria
    Article Snippet: Growth was analysed in Nunc Edge™ 96-well plates (Thermo Fisher) by OASTS using the oCelloScope™ instrument (Philips BioCell A/S, Allerød, Denmark). .. OASTS is a digital time-lapse microscopy technology that scans through a fluid sample, generating time-resolved series of images .

    Evaporation:

    Article Title: Growth and single cell kinetics of the loricate choanoflagellate Diaphanoeca grandis
    Article Snippet: In the oCelloScope, D. grandis was grown in NUNCTM Edge 96-well plates (Thermo Scientific, USA) containing 200 μL of sterilised seawater (salinity = 10‰, temp. .. To minimize evaporation, 8 mL of water was added to the surrounding plate-reservoir.

    Concentration Assay:

    Article Title: Assessment of Cr(VI)-Induced Cytotoxicity and Genotoxicity Using High Content Analysis
    Article Snippet: For assays in undifferentiated/proliferating Caco-2 cells, cells were seeded at a density of 1×104 cells/100 µl/well in Nunc Edge 96-well microplates (Thermo Fisher Scientific) 24 hr prior to incubation with compounds. .. Stock solutions of SDD and hydrogen peroxide were prepared in H2 O. Rotenone was prepared in DMSO at a final concentration of 0.25% DMSO.

    Fractionation:

    Article Title: Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies
    Article Snippet: Elution from the gel filtration column was carried out at a flow rate of 0.5 mL/min, fractionation volume of 0.3 mL, and total running time of 40 min. .. TYR enzyme activity assays were performed at 298 K using 3,4-dihydroxyphenylalanine (L-DOPA) as substrate in a Nunc™ Edge 96-Well plate (Thermo Fisher Scientific) [ , ].

    Prestoblue Assay:

    Article Title: Geometric constraints of endothelial cell migration on electrospun fibres
    Article Snippet: Cell viability was assessed using PrestoBlue® assay according to the manufacturer’s protocol (Life Technologies, cat. .. Three 100 µL media samples were transferred from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Article Title: Schwann cells promote endothelial cell migration
    Article Snippet: Cell viability was assessed using PrestoBlue® assay according to the manufacturer's protocol (Life Technologies, cat. .. Three 100 µL media samples were taken from each well into a Nunc™ 96-well plate (Thermo Scientific, cat.

    Lysis:

    Article Title: Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep
    Article Snippet: Briefly, successive two fold dilutions of 50 µL of sera, from 1/2 to 1/256 onwards, were made in 50 µL of Dulbecco’s Modified Eagles Medium (Gibco, Life Technologies, Ghent, Belgium) supplemented by 1000 IU penicillin/mL, 50 µg/mL gentamicin (Gibco, Life Technologies, Ghent, Belgium) and 250 µg/mL amphotericin B (Gibco, Life Technologies, Ghent, Belgium) (DMEM) in Nunc Edge 96-well plates (Thermo scientific, Waltham, MA, USA). .. After four days of incubation, the titre was determined as the reciprocal of the highest serum dilution in which no lysis plaques could be identified in the cell monolayer under the light microscope.

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    Thermo Fisher nunc 96 well plate
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