nunc easyflask cell culture flasks  (Thermo Fisher)


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    Name:
    Nunc EasYFlask Cell Culture Flasks
    Description:
    Enhance cell attachment growth and differentiation with Thermo Scientific Nunc Cell Culture Treated EasYFlasks These polystyrene flasks receive the proprietary Nunclon Delta surface treatment to maximize adhesion for a broad range of cell types The ergonomic design with angled neck and wide opening allows easy access to the growth surface by pipettes or cell scrapers
    Catalog Number:
    156340
    Price:
    None
    Applications:
    Cell Culture|Cell Culture Plastics|Laboratory Plastics and Supplies|Microplates, Dishes and Flasks
    Category:
    Lab Supplies Plastics Glassware
    Buy from Supplier


    Structured Review

    Thermo Fisher nunc easyflask cell culture flasks
    Enhance cell attachment growth and differentiation with Thermo Scientific Nunc Cell Culture Treated EasYFlasks These polystyrene flasks receive the proprietary Nunclon Delta surface treatment to maximize adhesion for a broad range of cell types The ergonomic design with angled neck and wide opening allows easy access to the growth surface by pipettes or cell scrapers
    https://www.bioz.com/result/nunc easyflask cell culture flasks/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc easyflask cell culture flasks - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Transfection:

    Article Title: Enhanced stress tolerance through reduction of G3BP and suppression of stress granules
    Article Snippet: .. For siRNA transfection, cultured spermatogonial clumps were separated from feeders by gentle pipetting and a single-cell suspension was generated by trypsin-EDTA digestion. .. 1×105 or 2×104 cells were plated in 24 or 96 well plates, respectively, without STO feeder cells in mSFM with GDNF and FGF2.

    Selection:

    Article Title: The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods
    Article Snippet: .. Liquid f/2 selection Transformant cultures were grown in 225 cm2 EasYFlask Nunc bottles (#159934) (Thermo Scientific, Waltham, Massachusetts, US) filled with 150 ml of filtered, autoclaved seawater enriched with f/2 medium. .. The selection antibiotic/herbicide used was either 100 μg/ml chloramphenicol or 150 ng/ml atrazine and the cultures were kept under standard growth conditions.

    Transferring:

    Article Title: Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA)
    Article Snippet: .. UV Spectrophotometer (e.g. NanoDrop 2000c, Thermo Fisher Scientific) FACS machine (e.g. FACS Aria II, BD Biosciences) Centrifuge for 15-ml and 50-ml Falcon tubes (e.g. Thermo Fisher Scientific Heraeus multifuge 3S+) Falcon tubes, polypropylene, 15 ml (BD Falcon, cat. no. 352097) Falcon tubes, polypropylene, 50 ml (BD Falcon, cat. no. 352070) Sterile p10; p100 and p1000 pipette tips with filters (Gilson DF10ST cat. no F171103; DF100ST cat. no. F171403 and DF1000ST cat. no. F171703) Standard microcentrifuge tubes, 1.5 ml (Eppendorf, cat. no. 0030 125.150) Tissue culture plate, 6 wells (Corning Falcon, cat. no. 353934) Tissue culture plate, 24 wells (Corning Falcon, cat. no. 353226) Tissue culture plate, 96 wells flat bottom (Corning Falcon, cat. no. 353227) Tissue culture dish, 100 mm (Corning Falcon, cat. no. 353003) Nunc EasYFlask 225 cm2 (T225 flask), filter cap, 70-ml working volume (Thermo Scientific, cat. no. 159934) Nunc EasYFlask 75 cm2 (T75 flask), filter cap, 25-ml working volume (Thermo Scientific, cat. no. 156499) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat.no. .. 4311971) 50 μm cup Filcons (BD Biosciences, cat. no. 340629) 5-ml round-bottom Falcon tubes (BD Biosciences, cat. no. 352054) PCR plates, 96 well (Sarstedt, cat. no. 72.1978.202) Axygen 8-Strip PCR tubes (Fischer Scientific, cat. no. 14-222-250) Thermocycler with programmable temperature stepping functionality, 96 well (e.g. Veriti Thermocycler cat. no. 4452300 or Eppendorf vapo-protect, cat. no. E950040015) Benchtop microcentrifuge (e.g. Eppendorf, Centrifuge 5417R) Eppendorf Thermomixer® R, dry block heating and cooling shaker (T3317) Gel electrophoresis system (PowerPac basic power supply, Bio-Rad, cat. no. 164–5050), and Sub-Cell GT System gel tray (Bio-Rad, cat. no. 170–4401) Novex XCell SureLock mini-cell (Life Technologies, cat. no. EI0001) Digital DNA gel imaging system (e.g GelDoc EZ, Bio-Rad, cat. no. 170–8270), and blue sample tray (Bio-Rad, cat. no. 170–8273) Blue-light transilluminator and orange filter goggles (SafeImager 2.0; Invitrogen, cat. no. G6600) ) Nucleofector 2b (Lonza) Fluorescence microscope for GFP

    Cell Culture:

    Article Title: Strong Components of Epigenetic Memory in Cultured Human Fibroblasts Related to Site of Origin and Donor Age
    Article Snippet: .. The supernatant was discarded, 5mL of fresh media was added to each conical tube, and the contents of the tubes were transferred onto separate 25 cm3 cell culture Easy Flasks (Thermo Scientific, Cat# 156367), where they were kept in cultures for 3–5 days in an incubator (at 37°C and 5% CO2 ). .. When the cells reached 90–95% confluence, the cells from each 25 cm3 flask were transferred onto two 75 cm3 cell culture easy flasks (Thermo Scientific, Cat# 156499) and kept in cultures for continued growth.

    Article Title: Enhanced stress tolerance through reduction of G3BP and suppression of stress granules
    Article Snippet: .. For siRNA transfection, cultured spermatogonial clumps were separated from feeders by gentle pipetting and a single-cell suspension was generated by trypsin-EDTA digestion. .. 1×105 or 2×104 cells were plated in 24 or 96 well plates, respectively, without STO feeder cells in mSFM with GDNF and FGF2.

    Article Title: Measurement of in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device
    Article Snippet: .. Stock MRC-5 cells are cultured in a humidified incubator at 37 °C with 5% CO2 using the growth medium in T25 cell culture flasks (Nunc 156367, Thermo Scientific Inc., Rochester, NY), and passaged by dissociation with TrypLE™ Express Enzyme (Gibco 12604, Invitrogen). .. After the control experiment, the cell culture channel is filled with distilled water and exposed under ultraviolet radiation for 30 minutes for sterilization.

    Article Title: Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes
    Article Snippet: .. The stocks were maintained in a T25 cell culture flask (Nunc 156367, Thermo Scientific Inc., Rochester, NY), and passaged by dissociation with 0.25% trypsin-EDTA (Gibco 25200, Invitrogen). ..

    Article Title: The functional and inflammatory response of brain endothelial cells to Toll-Like Receptor agonists
    Article Snippet: .. Cells were cultured at 37 °C in a dehumidified atmosphere of 5% CO2 on Nunc T75 flasks (Falcon, Cat# 156499) that had been coated with 1 µg/cm2 rat collagen (Gibco, Cat# A1048301) diluted in 0.02 M glacial acetic acid solution. .. Characterization of pharmacological responsiveness using xCELLigence technology hCMVEC responsiveness to a range of TLR ligands was analysed using the xCELLigence real time cell analyser (xCELLigence RTCA-SP system bundle, ACEA Biosciences Inc.).

    Article Title: Differential development of neuronal physiological responsiveness in two human neural stem cell lines
    Article Snippet: .. Cell culture Having established the cell lines as described, cells were routinely expanded for experimental work on laminin coated T75 cm2 or T175 cm2 tissue culture flasks (Nunc Easyflask) in B27 medium [DMEM:F12 containing B27 neural cell supplement mix (Gibco), L-Glutamine (2 mM, Gibco), heparin (10 Units/ml, Sigma) and Gentamicin (50 μg/ml, Gibco)] in the presence of bFGF (10 ng/ml, Invitrogen) and EGF (20 ng/ml, Sigma) – referred to as growth medium. ..

    Generated:

    Article Title: Enhanced stress tolerance through reduction of G3BP and suppression of stress granules
    Article Snippet: .. For siRNA transfection, cultured spermatogonial clumps were separated from feeders by gentle pipetting and a single-cell suspension was generated by trypsin-EDTA digestion. .. 1×105 or 2×104 cells were plated in 24 or 96 well plates, respectively, without STO feeder cells in mSFM with GDNF and FGF2.

    Spectrophotometry:

    Article Title: Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA)
    Article Snippet: .. UV Spectrophotometer (e.g. NanoDrop 2000c, Thermo Fisher Scientific) FACS machine (e.g. FACS Aria II, BD Biosciences) Centrifuge for 15-ml and 50-ml Falcon tubes (e.g. Thermo Fisher Scientific Heraeus multifuge 3S+) Falcon tubes, polypropylene, 15 ml (BD Falcon, cat. no. 352097) Falcon tubes, polypropylene, 50 ml (BD Falcon, cat. no. 352070) Sterile p10; p100 and p1000 pipette tips with filters (Gilson DF10ST cat. no F171103; DF100ST cat. no. F171403 and DF1000ST cat. no. F171703) Standard microcentrifuge tubes, 1.5 ml (Eppendorf, cat. no. 0030 125.150) Tissue culture plate, 6 wells (Corning Falcon, cat. no. 353934) Tissue culture plate, 24 wells (Corning Falcon, cat. no. 353226) Tissue culture plate, 96 wells flat bottom (Corning Falcon, cat. no. 353227) Tissue culture dish, 100 mm (Corning Falcon, cat. no. 353003) Nunc EasYFlask 225 cm2 (T225 flask), filter cap, 70-ml working volume (Thermo Scientific, cat. no. 159934) Nunc EasYFlask 75 cm2 (T75 flask), filter cap, 25-ml working volume (Thermo Scientific, cat. no. 156499) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat.no. .. 4311971) 50 μm cup Filcons (BD Biosciences, cat. no. 340629) 5-ml round-bottom Falcon tubes (BD Biosciences, cat. no. 352054) PCR plates, 96 well (Sarstedt, cat. no. 72.1978.202) Axygen 8-Strip PCR tubes (Fischer Scientific, cat. no. 14-222-250) Thermocycler with programmable temperature stepping functionality, 96 well (e.g. Veriti Thermocycler cat. no. 4452300 or Eppendorf vapo-protect, cat. no. E950040015) Benchtop microcentrifuge (e.g. Eppendorf, Centrifuge 5417R) Eppendorf Thermomixer® R, dry block heating and cooling shaker (T3317) Gel electrophoresis system (PowerPac basic power supply, Bio-Rad, cat. no. 164–5050), and Sub-Cell GT System gel tray (Bio-Rad, cat. no. 170–4401) Novex XCell SureLock mini-cell (Life Technologies, cat. no. EI0001) Digital DNA gel imaging system (e.g GelDoc EZ, Bio-Rad, cat. no. 170–8270), and blue sample tray (Bio-Rad, cat. no. 170–8273) Blue-light transilluminator and orange filter goggles (SafeImager 2.0; Invitrogen, cat. no. G6600) ) Nucleofector 2b (Lonza) Fluorescence microscope for GFP

    FACS:

    Article Title: Genome editing using FACS enrichment of nuclease expressing cells and Indel Detection by Amplicon Analysis (IDAA)
    Article Snippet: .. UV Spectrophotometer (e.g. NanoDrop 2000c, Thermo Fisher Scientific) FACS machine (e.g. FACS Aria II, BD Biosciences) Centrifuge for 15-ml and 50-ml Falcon tubes (e.g. Thermo Fisher Scientific Heraeus multifuge 3S+) Falcon tubes, polypropylene, 15 ml (BD Falcon, cat. no. 352097) Falcon tubes, polypropylene, 50 ml (BD Falcon, cat. no. 352070) Sterile p10; p100 and p1000 pipette tips with filters (Gilson DF10ST cat. no F171103; DF100ST cat. no. F171403 and DF1000ST cat. no. F171703) Standard microcentrifuge tubes, 1.5 ml (Eppendorf, cat. no. 0030 125.150) Tissue culture plate, 6 wells (Corning Falcon, cat. no. 353934) Tissue culture plate, 24 wells (Corning Falcon, cat. no. 353226) Tissue culture plate, 96 wells flat bottom (Corning Falcon, cat. no. 353227) Tissue culture dish, 100 mm (Corning Falcon, cat. no. 353003) Nunc EasYFlask 225 cm2 (T225 flask), filter cap, 70-ml working volume (Thermo Scientific, cat. no. 159934) Nunc EasYFlask 75 cm2 (T75 flask), filter cap, 25-ml working volume (Thermo Scientific, cat. no. 156499) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat.no. .. 4311971) 50 μm cup Filcons (BD Biosciences, cat. no. 340629) 5-ml round-bottom Falcon tubes (BD Biosciences, cat. no. 352054) PCR plates, 96 well (Sarstedt, cat. no. 72.1978.202) Axygen 8-Strip PCR tubes (Fischer Scientific, cat. no. 14-222-250) Thermocycler with programmable temperature stepping functionality, 96 well (e.g. Veriti Thermocycler cat. no. 4452300 or Eppendorf vapo-protect, cat. no. E950040015) Benchtop microcentrifuge (e.g. Eppendorf, Centrifuge 5417R) Eppendorf Thermomixer® R, dry block heating and cooling shaker (T3317) Gel electrophoresis system (PowerPac basic power supply, Bio-Rad, cat. no. 164–5050), and Sub-Cell GT System gel tray (Bio-Rad, cat. no. 170–4401) Novex XCell SureLock mini-cell (Life Technologies, cat. no. EI0001) Digital DNA gel imaging system (e.g GelDoc EZ, Bio-Rad, cat. no. 170–8270), and blue sample tray (Bio-Rad, cat. no. 170–8273) Blue-light transilluminator and orange filter goggles (SafeImager 2.0; Invitrogen, cat. no. G6600) ) Nucleofector 2b (Lonza) Fluorescence microscope for GFP

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  • 99
    Thermo Fisher nunc easyflask cell culture flasks
    Nunc Easyflask Cell Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc easyflask cell culture flasks/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nunc easyflask cell culture flasks - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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