nunc 96 well polypropylene microwell plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nunc 96 well polypropylene microwell plates
    Nunc 96 Well Polypropylene Microwell Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc 96 well polypropylene microwell plates/product/Thermo Fisher
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nunc 96 well polypropylene microwell plates - by Bioz Stars, 2020-04
    92/100 stars

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    Binding Assay:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: Paragraph title: MBL-IBV binding assay ... One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506).

    Purification:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: For the titration of MBL binding, concentrations of 0, 1, 2, 4, 8, 16, 24 and 32 g/mL purified MBL were used, and 5 L serum samples were diluted 1:50. .. One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: MBL-IBV binding assay The binding capacity of MBL to IBV was measured using ELISA. .. One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506).

    Concentration Assay:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: BioWhittaker ® Veronal buffer (Lonza, Walkersille, MD, USA; cat. no. 12-624E) adjusted to a final concentration of 5 mM MgCl 2 and 10 mM CaCl 2 was used for diluting the samples. .. One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506).

    Incubation:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: .. One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506). .. The plate was then incubated at room temperature for 1 h. After a washing step with Gibco ® DPBS supplemented with calcium and magnesium (Life Technologies Europe BV, Naerum, Denmark; cat. no. 14080-048) and pH adjusted to 7.4 followed by supplementation with 0.1% BSA (hereafter called PBS+), the wells were incubated for 45 min at room temperature with 1 g/mL of biotinylated monoclonal mouse anti-cMBL (BioPorto Diagnostics A/S, Gentofte, Denmark; cat. no. HYB 182-01) in PBS+.

    Titration:

    Article Title: Adjuvant effects of mannose-binding lectin ligands on the immune response to infectious bronchitis vaccine in chickens with high or low serum mannose-binding lectin concentrations.
    Article Snippet: For the titration of MBL binding, concentrations of 0, 1, 2, 4, 8, 16, 24 and 32 g/mL purified MBL were used, and 5 L serum samples were diluted 1:50. .. One-hundred microlitres of the dilutions and saccharides were mixed in a Nunc 96-well polypropylen MicroWell plate (Thermo Fisher Scientific, Slangerup, Denmark; cat. no. 442587) and incubated for 5 min before the samples were transferred to a 96-well microtitre plate coated with IBV antigen (from The ProFLOK ® IBV Antibody Test Kit from Synbiotics Corporation, San Diego, CA, USA; cat. no. 96-6506).

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    Thermo Fisher sterile nunctm microwelltm 96 well plates
    Sterile Nunctm Microwelltm 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile nunctm microwelltm 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    sterile nunctm microwelltm 96 well plates - by Bioz Stars, 2020-04
    99/100 stars
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