nuli 1  (ATCC)


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    ATCC nuli 1
    Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1  (ATCC)


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    ATCC nuli 1
    Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (Lonza)


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    Lonza nuli 1 cells
    Nuli 1 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1  (ATCC)


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    ATCC nuli 1
    Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1  (ATCC)


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    ATCC nuli 1
    Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (Lonza)


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    Lonza nuli 1 cells
    Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO <t>Nuli-1</t> cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.
    Nuli 1 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells"

    Article Title: Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells

    Journal: Journal of Virology

    doi: 10.1128/jvi.00906-23

    Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.
    Figure Legend Snippet: Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.

    Techniques Used: Infection, Expressing, Comparison, Plaque Assay, Virus

    nuli 1  (ATCC)


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    ATCC nuli 1
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    nuli 1  (Thermo Fisher)


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    Thermo Fisher nuli 1
    Nuli 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1  (Lonza)


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    Lonza nuli 1
    Nuli 1, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cell line  (ATCC)


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    ATCC nuli 1 cell line
    Nuli 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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