nuli 1 cells  (Lonza)


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    Lonza nuli 1 cells
    Nuli 1 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (Lonza)


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    Lonza nuli 1 cells
    Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO <t>Nuli-1</t> cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.
    Nuli 1 Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells - by Bioz Stars, 2024-10
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    1) Product Images from "Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells"

    Article Title: Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells

    Journal: Journal of Virology

    doi: 10.1128/jvi.00906-23

    Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.
    Figure Legend Snippet: Concurrent truncation of two glycan types decreases H1N1 but not H5N1 infection. ( A ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - and [NG] - DKO A549 cells. WT A549 and DKO cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin, and at 16 hpi, the % of GFP expressing cells was determined by flow cytometric analysis. Left: comparison of infection levels between WT A549 and [NO] - DKO cells; right: comparison of infection levels between WT A549 and [NG] - DKO cells. Data are presented as mean percentage of GFP+ cells from triplicate samples ± SD. ( B ) Multi-cycle replication assays with H1N1 and H5N1 viruses in [NO] - and [NG] - DKO cells. WT A549 and DKO cells seeded in 6-well dishes were infected at a low MOI with H1N1 (MOI = 0.01) or H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin and at 48 hpi, viral titers in the supernatants were determined by plaque assay in MDCK cells. Left: comparison of viral replication in WT and [NO] - DKO cells; right: comparison of viral replication in WT and [NG] - DKO cells. ( C ) Single-cycle infection assays with H1N1-GFP and H5N1-GFP in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected with H1N1-GFP or H5N1-GFP at a high MOI (MOI = 3) without TPCK-treated trypsin and at 16 hpi, the % of GFP positive cells was determined by flow cytometric analysis. Data are represented as mean percentage of GFP+ cells from triplicate samples ± SD. ( D ) Multi-cycle replication assays with H5N1 virus in [NO] - DKO Nuli-1 cells. WT and DKO Nuli-1 cells seeded in 6-well dishes were infected at a low MOI with H5N1 (MOI = 0.001) in the presence of TPCK-treated trypsin, and viral titers in the supernatants at different hpi were determined by plaque assay in MDCK cells. Data are represented as mean titer of triplicate samples ± SD (PFU/mL). *Denotes P -value ≤ 0.05. ns is non-significant. Data are representative of at least three independent experiments.

    Techniques Used: Infection, Expressing, Comparison, Plaque Assay, Virus

    nuli 1 cell line  (ATCC)


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    ATCC nuli 1 cell line
    Nuli 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (ATCC)


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    ATCC nuli 1 cells
    Nuli 1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (OriGene)


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    OriGene nuli 1 cells
    Nuli 1 Cells, supplied by OriGene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cell line  (ATCC)


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    ATCC nuli 1 cell line
    Overview of the methodology used for RNA samples acquisition from the complex mixture of <t>Nuli-1</t> epithelial cells-bacteria-phage, library preparation and Illumina RNA sequencing.
    Nuli 1 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Impact of phage predation on P. aeruginosa adhered to human airway epithelium: major transcriptomic changes in metabolism and virulence-associated genes"

    Article Title: Impact of phage predation on P. aeruginosa adhered to human airway epithelium: major transcriptomic changes in metabolism and virulence-associated genes

    Journal: RNA Biology

    doi: 10.1080/15476286.2023.2216065

    Overview of the methodology used for RNA samples acquisition from the complex mixture of Nuli-1 epithelial cells-bacteria-phage, library preparation and Illumina RNA sequencing.
    Figure Legend Snippet: Overview of the methodology used for RNA samples acquisition from the complex mixture of Nuli-1 epithelial cells-bacteria-phage, library preparation and Illumina RNA sequencing.

    Techniques Used: RNA Sequencing Assay

    Pseudomonas phage LUZ19 transcriptional landscape on P. aeruginosa PAO1 adhered to Nuli-1 epithelial cells. 5min samples, 10min samples and 15min samples represent the phage transcripts at the early, middle, and late infection stage, respectively.
    Figure Legend Snippet: Pseudomonas phage LUZ19 transcriptional landscape on P. aeruginosa PAO1 adhered to Nuli-1 epithelial cells. 5min samples, 10min samples and 15min samples represent the phage transcripts at the early, middle, and late infection stage, respectively.

    Techniques Used: Infection

    Principal component analysis of total transcriptomes from samples acquired at early (5min), middle (10min), and late infection (15min) in the presence of LB media, MCCM media, and Nuli-1 epithelial cells. The control samples (without phage presence) in each respective media are also included (t0). (Figure contains data described in (24)).
    Figure Legend Snippet: Principal component analysis of total transcriptomes from samples acquired at early (5min), middle (10min), and late infection (15min) in the presence of LB media, MCCM media, and Nuli-1 epithelial cells. The control samples (without phage presence) in each respective media are also included (t0). (Figure contains data described in (24)).

    Techniques Used: Infection

    Representation of Log2Fold values for each categorical group of genes at different stages of infection (5-, 10-, and 15- vs. 0min) in bacteria adhered to Nuli-1 epithelial cells monolayer, grown on MCCM media, and grown on LB media. Log2Fold values are always relative to the expression level of uninfected bacteria at each respective growth condition. Plot was performed in GraphPad prism v8.0.1 with double gradient colourmap, using red for smallest value (Log2Fold = −6), yellow for baseline value (Log2Fold = 0), and green for largest value (Log2Fold = 6).
    Figure Legend Snippet: Representation of Log2Fold values for each categorical group of genes at different stages of infection (5-, 10-, and 15- vs. 0min) in bacteria adhered to Nuli-1 epithelial cells monolayer, grown on MCCM media, and grown on LB media. Log2Fold values are always relative to the expression level of uninfected bacteria at each respective growth condition. Plot was performed in GraphPad prism v8.0.1 with double gradient colourmap, using red for smallest value (Log2Fold = −6), yellow for baseline value (Log2Fold = 0), and green for largest value (Log2Fold = 6).

    Techniques Used: Infection, Expressing

    Representation of Log2Fold values for each group of genes that are specifically targeted at Nuli-1 at different stages of infection (5-, 10-, and 15- vs. 0min) in bacteria adhered to Nuli-1 epithelial cells monolayer, grown on MCCM media, and grown on LB media. Log2Fold values are always relative to the expression level of uninfected bacteria at each respective growth condition. Plot was performed in GraphPad Prism v8.0.1 with double gradient colourmap, using red for smallest value (Log2Fold = −6), yellow for baseline value (Log2Fold = 0), and green for largest value (Log2Fold = 6).
    Figure Legend Snippet: Representation of Log2Fold values for each group of genes that are specifically targeted at Nuli-1 at different stages of infection (5-, 10-, and 15- vs. 0min) in bacteria adhered to Nuli-1 epithelial cells monolayer, grown on MCCM media, and grown on LB media. Log2Fold values are always relative to the expression level of uninfected bacteria at each respective growth condition. Plot was performed in GraphPad Prism v8.0.1 with double gradient colourmap, using red for smallest value (Log2Fold = −6), yellow for baseline value (Log2Fold = 0), and green for largest value (Log2Fold = 6).

    Techniques Used: Infection, Expressing

    human airway epithelial cells nuli 1  (ATCC)


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    ATCC human airway epithelial cells nuli 1
    Human Airway Epithelial Cells Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (Thermo Fisher)


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    Thermo Fisher nuli 1 cells
    Nuli 1 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human airway epithelial cells nuli 1  (ATCC)


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    ATCC human airway epithelial cells nuli 1
    Human Airway Epithelial Cells Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human airway epithelial cell line nuli 1  (ATCC)


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    ATCC human airway epithelial cell line nuli 1
    Human Airway Epithelial Cell Line Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (OriGene)
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    human airway epithelial cells nuli 1  (ATCC)
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    ATCC human airway epithelial cells nuli 1
    Human Airway Epithelial Cells Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nuli 1 cells  (Thermo Fisher)
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    Thermo Fisher nuli 1 cells
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    human airway epithelial cell line nuli 1  (ATCC)
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    ATCC human airway epithelial cell line nuli 1
    Human Airway Epithelial Cell Line Nuli 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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