mant dgdp  (Jena Bioscience)


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Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
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  • 85
    Name:
    Mant dGDP
    Description:

    Catalog Number:
    nu-205l
    Price:
    352.15
    Applications:
    Resonance energy transfer to Trp in protein cdc42hs[1] Conformational analysis of Ras-like protein[2]
    Purity:
    ≥ 95 % (HPLC)
    Category:
    Nucleotides Nucleosides
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    Structured Review

    Jena Bioscience mant dgdp

    https://www.bioz.com/result/mant dgdp/product/Jena Bioscience
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mant dgdp - by Bioz Stars, 2020-08
    85/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions
    Article Snippet: .. The corresponding recombinantly expressed, full-length K-Ras protein (G12C mutant or G12C mutant labelled fully with either compound 8 or 12 ) at about 10 μM concentration was incubated with 200 μM mant-dGDP (Jena Biosciences) in the presence of 2.5 M EDTA. .. After 1 h at room temperature, MgCl2 to a final concentration of 10 mM was added.

    Concentration Assay:

    Article Title: K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions
    Article Snippet: .. The corresponding recombinantly expressed, full-length K-Ras protein (G12C mutant or G12C mutant labelled fully with either compound 8 or 12 ) at about 10 μM concentration was incubated with 200 μM mant-dGDP (Jena Biosciences) in the presence of 2.5 M EDTA. .. After 1 h at room temperature, MgCl2 to a final concentration of 10 mM was added.

    Incubation:

    Article Title: K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions
    Article Snippet: .. The corresponding recombinantly expressed, full-length K-Ras protein (G12C mutant or G12C mutant labelled fully with either compound 8 or 12 ) at about 10 μM concentration was incubated with 200 μM mant-dGDP (Jena Biosciences) in the presence of 2.5 M EDTA. .. After 1 h at room temperature, MgCl2 to a final concentration of 10 mM was added.

    other:

    Article Title: K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions
    Article Snippet: In brief, the respective full-length proteins were loaded with mant-dGDP (see above).

    Fluorescence:

    Article Title: Structural analysis of autoinhibition in the Ras-specific exchange factor RasGRP1
    Article Snippet: .. Nucleotide exchange measurements were measured by mant-dGDP (Jena Bioscience, Jena, Germany) fluorescence using a stopped-flow apparatus with a Fluoromax-3 fluorometer (Horiba Scientific, Edison, NJ) and analyzed as described ( ). .. Samples were excited at 370 nm (5 nm slit width) and the emission at 430 nm (5 nm slit width) was followed for at least eight minutes with a 0.5 s sampling interval.

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  • 85
    Jena Bioscience mant dgdp
    Mant Dgdp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mant dgdp/product/Jena Bioscience
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mant dgdp - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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