nsi source  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nsi source
    Nsi Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsi source/product/Thermo Fisher
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    nsi source - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: Peptides were labeled using TMT kit and then separated by high-performance liquid chromatography (HPLC), and combined into 18 fractions. .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Article Title: S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome, et al. S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome
    Article Snippet: The tryptic peptides were fractionated into fractions by high pH reverse‐phase HPLC using Agilent 300Extend C18 column (5 μm particles, 4.6 mm ID, 250 mm length). .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC.

    Flow Cytometry:

    Article Title: Auxin-to-Gibberellin Ratio as a Signal for Light Intensity and Quality in Regulating Soybean Growth and Matter Partitioning
    Article Snippet: The gradient increased from 7 to 25% solvent B (0.1% FA in 98% ACN) in over 26 min, from 25 to 38% in 8 min, to 80% in 3 min, and then held at 80% for the last 3 min, all at a constant flow rate of 350 nL/min on an EASY-nLC 1000 UPLC system. .. The peptides were subjected to NSI source and tandem mass spectrometry (MS/MS) in Orbitrap FusionTM TribridTM (Thermo) coupled online with UPLC.

    Article Title: Discovery of a highly selective JAK3 inhibitor for the treatment of rheumatoid arthritis
    Article Snippet: Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, Thermo Scientific) with a linear gradient of 8–35% solvent B (0.1% FA in 98% ACN) for 12 min and 35–80% solvent B for 4 min at a constant flow rate of 320 nl/min on an EASY-nLC 1000 UPLC system. .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC.

    Article Title: Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
    Article Snippet: Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, Thermo Fisher Scientific) with a linear gradient of 4–22% solvent B (0.1% FA in 98% ACN) for 50 min, 22–35% solvent B for 12 min, 35–80% solvent B for 4 min, and holding at 80% for the last 4 min, all at a constant flow rate of 400 nL/min, on an EASY-nLC 1000 UPLC system. .. Peptides were subjected to an NSI source followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific) coupled online to the UPLC.

    Article Title: Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Article Snippet: The gradient was composed of an increase from 6 to 23% in 0.1% formic acid in 98% acetonitrile over 26 min, 23–35% in 8 min, and climbing to 80% in 3 min then holding at 80% for the last 3 min, at a constant flow rate of 400 nl/min on an EASY-nLC 1000 UPLC system. .. The peptides were then subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific, USA) coupled online to the UPLC.

    Article Title: Comprehensive analysis of lysine crotonylation in proteome of maintenance hemodialysis patients
    Article Snippet: All steps were performed at a constant flow rate of 400 nL/min on an EASY-nLC 1000 ultra-performance liquid chromatography (UPLC) system. .. The peptides were subjected to NSI source, followed by MS/MS in Q ExactiveTM Plus (Thermo) coupled online to UPLC.

    Article Title: ING5 differentially regulates protein lysine acetylation and promotes p300 autoacetylation
    Article Snippet: LC-MS/MS analysis Peptides were dissolved in 0.1% FA, and peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, Thermo Scientific) with a linear gradient of 5–35% solvent B (0.1% FA in 98% ACN) for 30 min and 35–80% solvent B for 10 min at a constant flow rate of 300 nl/min on an EASY-nLC 1000 UPLC system, The resulting peptides were analyzed by Q ExactiveTM Plus hybrid quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific). .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC.

    Article Title: Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa
    Article Snippet: The column was maintained at a constant flow rate of 400 nl/min on an EASY-nLC 1000 UPLC system. .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) with the use of Q Exactive TM plus (Thermo) coupled online to the UPLC.

    Article Title: Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenic Thermoanaerobacterium sp. M5
    Article Snippet: The flow rate was maintained at 380 nL/min on an EASY-nLC 1000 UPLC system. .. The resolved peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC.

    Article Title: S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome, et al. S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome
    Article Snippet: The gradient was comprised of an increase from 6% to 23% solvent B (0.1% formic acid in 98% acetonitrile) over 26 minutes, 23%‐35% in 8 minutes and climbing to 80% in 3 minutes then holding at 80% for the last 3 minutes, all at a constant flow rate of 400 nL/min on an EASY‐nLC 1000 UPLC system. .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC.

    Chromatography:

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: Paragraph title: Tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis ... The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Protease Inhibitor:

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: At 48 hours, protein was extracted 48 hours after transfection by using lysis buffer with 1% protease inhibitor cocktail (Sigma), and reduced with 5 mM dithiothreitol for 30 min at 56ºC and 11 mM iodoacetamide for 15 min at room temperature in darkness. .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Transfection:

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: At 48 hours, protein was extracted 48 hours after transfection by using lysis buffer with 1% protease inhibitor cocktail (Sigma), and reduced with 5 mM dithiothreitol for 30 min at 56ºC and 11 mM iodoacetamide for 15 min at room temperature in darkness. .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Labeling:

    Article Title: New Insights into Regulation of Proteome and Polysaccharide in Cell Wall of Elsholtzia splendens in Response to Copper Stress
    Article Snippet: LC–ESI–MS/MS analysis by Q Exactive The labeled peptide was resuspended in buffer A (2% ACN, 0.1% FA) and centrifuged at 20,000×g for 2 min. .. The peptides were subjected to NSI source, followed by tandem mass spectrometry (MS/MS) in Q Exactive (Thermo) coupled online to the UPLC.

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: Paragraph title: Tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis ... The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Incubation:

    Article Title: Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Article Snippet: The suspended samples were then mixed with anti-Kac pan antibody-conjugated agarose beads (PTM Bio, China) and incubated at 4 °C overnight. .. The peptides were then subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific, USA) coupled online to the UPLC.

    Mass Spectrometry:

    Article Title: Auxin-to-Gibberellin Ratio as a Signal for Light Intensity and Quality in Regulating Soybean Growth and Matter Partitioning
    Article Snippet: .. The peptides were subjected to NSI source and tandem mass spectrometry (MS/MS) in Orbitrap FusionTM TribridTM (Thermo) coupled online with UPLC. ..

    Article Title: Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall
    Article Snippet: .. The resulting peptides were subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in Q Exactive™ (Thermo Fisher Scientific), coupled online to the UPLC. ..

    Article Title: Discovery of a highly selective JAK3 inhibitor for the treatment of rheumatoid arthritis
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC. ..

    Article Title: Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
    Article Snippet: .. Peptides were subjected to an NSI source followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific) coupled online to the UPLC. ..

    Article Title: Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Article Snippet: .. The peptides were then subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific, USA) coupled online to the UPLC. ..

    Article Title: New Insights into Regulation of Proteome and Polysaccharide in Cell Wall of Elsholtzia splendens in Response to Copper Stress
    Article Snippet: .. The peptides were subjected to NSI source, followed by tandem mass spectrometry (MS/MS) in Q Exactive (Thermo) coupled online to the UPLC. ..

    Article Title: ING5 differentially regulates protein lysine acetylation and promotes p300 autoacetylation
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC. .. For MS scans, the m/z scan range was 350 to 1600Da.

    Article Title: Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) with the use of Q Exactive TM plus (Thermo) coupled online to the UPLC. ..

    Article Title: Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenic Thermoanaerobacterium sp. M5
    Article Snippet: .. The resolved peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC. .. The resulting MS/MS data were processed using Maxquant search engine (v1.5.2.8).

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC). ..

    Article Title: S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome, et al. S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC. .. The resulting MS/MS data were processed using MaxQuant search engine (v.1.5.2.8).

    Tandem Mass Spectroscopy:

    Article Title: Auxin-to-Gibberellin Ratio as a Signal for Light Intensity and Quality in Regulating Soybean Growth and Matter Partitioning
    Article Snippet: .. The peptides were subjected to NSI source and tandem mass spectrometry (MS/MS) in Orbitrap FusionTM TribridTM (Thermo) coupled online with UPLC. ..

    Article Title: Biochemical and proteomics analyses of antioxidant enzymes reveal the potential stress tolerance in Rhododendron chrysanthum Pall
    Article Snippet: .. The resulting peptides were subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in Q Exactive™ (Thermo Fisher Scientific), coupled online to the UPLC. ..

    Article Title: Discovery of a highly selective JAK3 inhibitor for the treatment of rheumatoid arthritis
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC. ..

    Article Title: Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers
    Article Snippet: .. Peptides were subjected to an NSI source followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific) coupled online to the UPLC. ..

    Article Title: Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence
    Article Snippet: .. The peptides were then subjected to an NSI source, followed by tandem mass spectrometry (MS/MS) in a Q Exactive Plus (Thermo Fisher Scientific, USA) coupled online to the UPLC. ..

    Article Title: New Insights into Regulation of Proteome and Polysaccharide in Cell Wall of Elsholtzia splendens in Response to Copper Stress
    Article Snippet: .. The peptides were subjected to NSI source, followed by tandem mass spectrometry (MS/MS) in Q Exactive (Thermo) coupled online to the UPLC. ..

    Article Title: Comprehensive analysis of lysine crotonylation in proteome of maintenance hemodialysis patients
    Article Snippet: .. The peptides were subjected to NSI source, followed by MS/MS in Q ExactiveTM Plus (Thermo) coupled online to UPLC. ..

    Article Title: ING5 differentially regulates protein lysine acetylation and promotes p300 autoacetylation
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC. .. For MS scans, the m/z scan range was 350 to 1600Da.

    Article Title: Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) with the use of Q Exactive TM plus (Thermo) coupled online to the UPLC. ..

    Article Title: Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenic Thermoanaerobacterium sp. M5
    Article Snippet: .. The resolved peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC. .. The resulting MS/MS data were processed using Maxquant search engine (v1.5.2.8).

    Article Title: S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome, et al. S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome
    Article Snippet: .. The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC. .. The resulting MS/MS data were processed using MaxQuant search engine (v.1.5.2.8).

    Lysis:

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: At 48 hours, protein was extracted 48 hours after transfection by using lysis buffer with 1% protease inhibitor cocktail (Sigma), and reduced with 5 mM dithiothreitol for 30 min at 56ºC and 11 mM iodoacetamide for 15 min at room temperature in darkness. .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC).

    Liquid Chromatography:

    Article Title: Comprehensive analysis of lysine crotonylation in proteome of maintenance hemodialysis patients
    Article Snippet: All steps were performed at a constant flow rate of 400 nL/min on an EASY-nLC 1000 ultra-performance liquid chromatography (UPLC) system. .. The peptides were subjected to NSI source, followed by MS/MS in Q ExactiveTM Plus (Thermo) coupled online to UPLC.

    Article Title: Inhibition of Heme Oxygenase-1 enhances hyperthermia-induced autophagy and antiviral effect
    Article Snippet: .. The peptides were then subjected to NSI source followed by tandem mass spectrometry in Orbitrap FusionTM LumosTM TribridTM (Thermo) coupled online to the Ultra Performance Liquid Chromatography (UPLC). ..

    Derivative Assay:

    Article Title: S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome, et al. S100‐A9 protein in exosomes derived from follicular fluid promotes inflammation via activation of NF‐κB pathway in polycystic ovary syndrome
    Article Snippet: The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q Exactive™ Plus (Thermo) coupled online to the UPLC. .. The gene ontology analysis was derived from the UniProt‐GOA database ( http://www.http://www.ebi.ac.uk/GOA/ ) and GO annotation ( http://geneontology.org/ ).

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher nsi source
    Nsi Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsi source/product/Thermo Fisher
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    nsi source - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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