vahts universal v6 rna seq library prep kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co vahts universal v6 rna seq library prep kit
    <t>scRNA-seq</t> analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.
    Vahts Universal V6 Rna Seq Library Prep Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vahts universal v6 rna seq library prep kit/product/Vazyme Biotech Co
    Average 94 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    vahts universal v6 rna seq library prep kit - by Bioz Stars, 2022-11
    94/100 stars

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    1) Product Images from "m6A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina"

    Article Title: m6A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina

    Journal: bioRxiv

    doi: 10.1101/2022.05.08.491092

    scRNA-seq analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.
    Figure Legend Snippet: scRNA-seq analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.

    Techniques Used: Expressing, Mutagenesis

    Mouse retinal m 6 A epitranscriptome (A) Pie graph illustrating the number of genes carrying m 6 A modification detected by MeRIP-seq in the mouse retina at different ages. The numbers represent the number of genes in the respective pie region. (B) Plots illustrating the distribution of m 6 A peaks along mouse retinal transcripts. (C) The most enriched motif among m 6 A peak sequences in the mouse retina transcriptome. (D) IGV views illustrating the m 6 A peak distribution along transcripts of important retinal regulatory genes revealed by MeRIP-seq. (E) Bubble plot showing the biological processes enriched in genes carrying m 6 A modification detected in the retinas of mice at all ages.
    Figure Legend Snippet: Mouse retinal m 6 A epitranscriptome (A) Pie graph illustrating the number of genes carrying m 6 A modification detected by MeRIP-seq in the mouse retina at different ages. The numbers represent the number of genes in the respective pie region. (B) Plots illustrating the distribution of m 6 A peaks along mouse retinal transcripts. (C) The most enriched motif among m 6 A peak sequences in the mouse retina transcriptome. (D) IGV views illustrating the m 6 A peak distribution along transcripts of important retinal regulatory genes revealed by MeRIP-seq. (E) Bubble plot showing the biological processes enriched in genes carrying m 6 A modification detected in the retinas of mice at all ages.

    Techniques Used: Modification, Mouse Assay

    RNA-seq analyses of the transcriptomes of control and Mettl3 CKO retinas (A) PCA map illustrating separation of the control and Mettl3 CKO retinal transcriptomes. (B) Volcano plot illustrating the gene expression differences between control and Mettl3 CKO retinas. (C) Pearson scores of the transcriptomes of different samples. (D) Bar graph showing the biological processes enriched in the gene group that were downregulated in Mettl3 CKO retinas.
    Figure Legend Snippet: RNA-seq analyses of the transcriptomes of control and Mettl3 CKO retinas (A) PCA map illustrating separation of the control and Mettl3 CKO retinal transcriptomes. (B) Volcano plot illustrating the gene expression differences between control and Mettl3 CKO retinas. (C) Pearson scores of the transcriptomes of different samples. (D) Bar graph showing the biological processes enriched in the gene group that were downregulated in Mettl3 CKO retinas.

    Techniques Used: RNA Sequencing Assay, Expressing

    m 6 A-modified RPC-enriched genes (A) IGV views illustrating the m 6 A peak distribution along transcripts of RPC-enriched genes as revealed by MeRIP-seq. (B) MeRIP-qPCR results revealing downregulation of m 6 A modification of the transcripts in Mettl3 CKO retinas. (C) Transcript half-life measurements of the RPC-enriched genes carrying m 6 A modification. The data are presented as the means ± standard deviations, corresponding to three independent biological replicates. * p
    Figure Legend Snippet: m 6 A-modified RPC-enriched genes (A) IGV views illustrating the m 6 A peak distribution along transcripts of RPC-enriched genes as revealed by MeRIP-seq. (B) MeRIP-qPCR results revealing downregulation of m 6 A modification of the transcripts in Mettl3 CKO retinas. (C) Transcript half-life measurements of the RPC-enriched genes carrying m 6 A modification. The data are presented as the means ± standard deviations, corresponding to three independent biological replicates. * p

    Techniques Used: Modification, Real-time Polymerase Chain Reaction

    scRNA-seq analysis of Mettl3 CKO retinal cells (A) Expression patterns of marker genes of various types of retinal cells in UMAP. (B) Pie charts illustrating the cell type compositions in control and Mettl3 CKO retinas.
    Figure Legend Snippet: scRNA-seq analysis of Mettl3 CKO retinal cells (A) Expression patterns of marker genes of various types of retinal cells in UMAP. (B) Pie charts illustrating the cell type compositions in control and Mettl3 CKO retinas.

    Techniques Used: Expressing, Marker

    2) Product Images from "Differential transcriptomic landscapes of multiple organs from SARS-CoV-2 early infected rhesus macaques"

    Article Title: Differential transcriptomic landscapes of multiple organs from SARS-CoV-2 early infected rhesus macaques

    Journal: Research square

    doi: 10.21203/rs.3.rs-403788/v1

    Functional clusters of genes were identified by RNA-seq in multiple organs of rhesus macaque. a, Schematic diagram displaying 14 organs of rhesus macaque used in this study. b, c, Violin plots showing expression profiling for specific tissue marker genes of lung (b) and kidney (c) in all of 14 organs. d, Principal component analysis of gene expression patterns for 14 organs. e, Line plot showing the normalized expression pattern for genes from Cluster 1, which is determined by K-means clustering analysis. The light lines represent normalized expression value for each gene from Cluster 1, while dark line represents mean of normalized expression value among all Cluster 1 genes. f, Barplot showing the enriched GO (Gene Ontology) terms for Cluster 1. g, Line plot showing the normalized expression pattern for genes from Cluster 2, which is determined by K-means clustering analysis. The light lines represent normalized expression value for each gene from Cluster 2, while the dark line represents mean of normalized expression value among all Cluster 2 genes. h, Barplot showing the enriched GO (Gene Ontology) terms for Cluster 2.
    Figure Legend Snippet: Functional clusters of genes were identified by RNA-seq in multiple organs of rhesus macaque. a, Schematic diagram displaying 14 organs of rhesus macaque used in this study. b, c, Violin plots showing expression profiling for specific tissue marker genes of lung (b) and kidney (c) in all of 14 organs. d, Principal component analysis of gene expression patterns for 14 organs. e, Line plot showing the normalized expression pattern for genes from Cluster 1, which is determined by K-means clustering analysis. The light lines represent normalized expression value for each gene from Cluster 1, while dark line represents mean of normalized expression value among all Cluster 1 genes. f, Barplot showing the enriched GO (Gene Ontology) terms for Cluster 1. g, Line plot showing the normalized expression pattern for genes from Cluster 2, which is determined by K-means clustering analysis. The light lines represent normalized expression value for each gene from Cluster 2, while the dark line represents mean of normalized expression value among all Cluster 2 genes. h, Barplot showing the enriched GO (Gene Ontology) terms for Cluster 2.

    Techniques Used: Functional Assay, RNA Sequencing Assay, Expressing, Marker

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    Vazyme Biotech Co vahts universal v6 rna seq library prep kit
    <t>scRNA-seq</t> analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.
    Vahts Universal V6 Rna Seq Library Prep Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vahts universal v6 rna seq library prep kit/product/Vazyme Biotech Co
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vahts universal v6 rna seq library prep kit - by Bioz Stars, 2022-11
    94/100 stars
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    scRNA-seq analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.

    Journal: bioRxiv

    Article Title: m6A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina

    doi: 10.1101/2022.05.08.491092

    Figure Lengend Snippet: scRNA-seq analyses reveal distorted transcriptomes of late RPCs and Müller glial cells in Mettl3 CKO retinas (A) UMAP presenting the cell clusters of all sequenced cells. (B) Heatmap illustrating the expression patterns of key markers of each retinal cell type. (C) UMAP presenting the distribution of control and Mettl3 CKO retinal cells. (D) Bar column presenting the cell compositions in control and Mettl3 CKO retinas. (E) Left: pseudotime presentation illustrating the developmental progression from RPCs to Müller glia; right: cell density distributions of RPCs and Müller glia over the pseudotime period. (F) Left: separate pseudotime presentations of control and Mettl3 CKO RPCs and Müller glia; right: cell density distributions of control and Mettl3 CKO cells over the pseudotime period. (G) UMAP of reclustered RPCs. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO RPCs. (H) Volcano plot showing gene expression differences between mutant RPCs (encircled by the red dotted line in A) and the remaining RPCs. (I) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant RPC cluster. (J) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant RPC cluster. (K) UMAP of reclustered Müller glia. The red dotted line encircles a distinct cell cluster composed solely of Mettl3 CKO Müller glia. (L) Volcano plot showing gene expression differences between mutant Müller glia (encircled by the red dotted line in E) and the remaining Müller glia. (M) Bubble plot showing the biological processes enriched in genes that were upregulated in the Mettl3 -mutant Müller glial cluster. (N) Bubble plot showing the biological processes enriched in genes that were downregulated in the Mettl3 -mutant Müller glial cluster.

    Article Snippet: After recovery of precipitated m6 A-mRNA, sequencing libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme).

    Techniques: Expressing, Mutagenesis

    Mouse retinal m 6 A epitranscriptome (A) Pie graph illustrating the number of genes carrying m 6 A modification detected by MeRIP-seq in the mouse retina at different ages. The numbers represent the number of genes in the respective pie region. (B) Plots illustrating the distribution of m 6 A peaks along mouse retinal transcripts. (C) The most enriched motif among m 6 A peak sequences in the mouse retina transcriptome. (D) IGV views illustrating the m 6 A peak distribution along transcripts of important retinal regulatory genes revealed by MeRIP-seq. (E) Bubble plot showing the biological processes enriched in genes carrying m 6 A modification detected in the retinas of mice at all ages.

    Journal: bioRxiv

    Article Title: m6A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina

    doi: 10.1101/2022.05.08.491092

    Figure Lengend Snippet: Mouse retinal m 6 A epitranscriptome (A) Pie graph illustrating the number of genes carrying m 6 A modification detected by MeRIP-seq in the mouse retina at different ages. The numbers represent the number of genes in the respective pie region. (B) Plots illustrating the distribution of m 6 A peaks along mouse retinal transcripts. (C) The most enriched motif among m 6 A peak sequences in the mouse retina transcriptome. (D) IGV views illustrating the m 6 A peak distribution along transcripts of important retinal regulatory genes revealed by MeRIP-seq. (E) Bubble plot showing the biological processes enriched in genes carrying m 6 A modification detected in the retinas of mice at all ages.

    Article Snippet: After recovery of precipitated m6 A-mRNA, sequencing libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme).

    Techniques: Modification, Mouse Assay

    m 6 A-modified RPC-enriched genes (A) IGV views illustrating the m 6 A peak distribution along transcripts of RPC-enriched genes as revealed by MeRIP-seq. (B) MeRIP-qPCR results revealing downregulation of m 6 A modification of the transcripts in Mettl3 CKO retinas. (C) Transcript half-life measurements of the RPC-enriched genes carrying m 6 A modification. The data are presented as the means ± standard deviations, corresponding to three independent biological replicates. * p

    Journal: bioRxiv

    Article Title: m6A epitranscriptomic modification regulates neural progenitor-to-glial cell transition in the retina

    doi: 10.1101/2022.05.08.491092

    Figure Lengend Snippet: m 6 A-modified RPC-enriched genes (A) IGV views illustrating the m 6 A peak distribution along transcripts of RPC-enriched genes as revealed by MeRIP-seq. (B) MeRIP-qPCR results revealing downregulation of m 6 A modification of the transcripts in Mettl3 CKO retinas. (C) Transcript half-life measurements of the RPC-enriched genes carrying m 6 A modification. The data are presented as the means ± standard deviations, corresponding to three independent biological replicates. * p

    Article Snippet: After recovery of precipitated m6 A-mRNA, sequencing libraries were constructed using the VAHTS Universal V6 RNA-seq Library Prep Kit (Vazyme).

    Techniques: Modification, Real-time Polymerase Chain Reaction