Structured Review

Millipore novablue
Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/novablue/product/Millipore
Average 89 stars, based on 1 article reviews
Price from $9.99 to $1999.99
novablue - by Bioz Stars, 2020-05
89/100 stars

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Clone Assay:

Article Title: Roles of Cysteine Proteases Cwp84 and Cwp13 in Biogenesis of the Cell Wall of Clostridium difficile ▿ ▿ †
Article Snippet: .. E. coli strains Top10 (Invitrogen) and NovaBlue (Novagen) were used routinely for cloning and propagation of plasmids. ..

Article Title: Association of Mycobacterium Proteins with Lipid Droplets
Article Snippet: .. Escherichia coli strains DH5α, Top10, (Thermo Fisher Scientific, Waltham, MA), and NovaBlue (MilliporeSigma, Burlington, MA) were used for cloning. .. Protein purification was carried out in E. coil strain BL21(DE3) containing plasmid pLysS.

Article Title: Identification of a Novel Streptococcal Adhesin P (SadP) Protein Recognizing Galactosyl-?1-4-galactose-containing Glycoconjugates
Article Snippet: .. After cloning into pET28a and transformation into E. coli DH5α, plasmids were sequenced and were then transformed to the BL21(DE3) or Novablue(DE3) expression strains (Novagen). .. The protein was produced in E. coli by growing the bacteria to an A 550 of 0.5, inducing with 1 m m IPTG, and growing for 3 h. Harvested bacteria were stored at −70 °C.

Selection:

Article Title: Assembling the glycopeptide antibiotic scaffold: The biosynthesis of from Streptomyces toyocaensis NRRL15009
Article Snippet: .. Escherichia coli SURE2 (Stratagene), NovaBlue (Novagen), and TOP10 (Invitrogen) were grown in LB broth at 37°C with either ampicillin (final concentration 100 μg/ml) or apramycin selection (50 μg/ml), as appropriate. .. Genomic DNA was isolated by modification of existing methodology ( ).

Ligation:

Article Title: CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli
Article Snippet: .. Following ligation, pCR2.1 and pCDF-1b-derived plasmids were transformed in TOP10 (Invitrogen) or NovaBlue (Novagen) strains, respectively, and constructions were verified by sequencing with primers T7 (pCR2.1 and pCDF-1b) and M13R (pCR2.1) or T7t (pCDF-1b). .. Detection of CRISPR, leader and PAM sequences Leaders of ECOR strains were determined by sequencing PCR amplification products as described by Diez-Villaseñor et al. CRISPR and leaders in available E. coli genomes were identified with the CRISPRFinder program ( www.crispr.u-psud.fr/Server/ ).

Isolation:

Article Title: Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold
Article Snippet: .. The isolated DNA was further transformed into Novablue™ (E. coli ) cells (EMD Biosciences, San Diego, CA). .. A Qiagen miniprep kit was used to isolate plasmids from E.coli (Qiagen, Valencia, CA) and sequenced using the primer 5′ ACT ACG CTC TGC AGG CTA GT 3′.

Concentration Assay:

Article Title: Assembling the glycopeptide antibiotic scaffold: The biosynthesis of from Streptomyces toyocaensis NRRL15009
Article Snippet: .. Escherichia coli SURE2 (Stratagene), NovaBlue (Novagen), and TOP10 (Invitrogen) were grown in LB broth at 37°C with either ampicillin (final concentration 100 μg/ml) or apramycin selection (50 μg/ml), as appropriate. .. Genomic DNA was isolated by modification of existing methodology ( ).

other:

Article Title: The NanI and NanJ Sialidases of Clostridium perfringens Are Not Essential for Virulence
Article Snippet: The Escherichia coli cultures were derivatives of DH5α (Life Technologies) or NovaBlue (Novagen) and were propagated in 2× YT medium ( ) supplemented with ampicillin (100 μg/ml), chloramphenicol (30 μg/ml), erythromycin (150 μg/ml), or tetracycline (10 μg/ml).

Expressing:

Article Title: Identification of a Novel Streptococcal Adhesin P (SadP) Protein Recognizing Galactosyl-?1-4-galactose-containing Glycoconjugates
Article Snippet: .. After cloning into pET28a and transformation into E. coli DH5α, plasmids were sequenced and were then transformed to the BL21(DE3) or Novablue(DE3) expression strains (Novagen). .. The protein was produced in E. coli by growing the bacteria to an A 550 of 0.5, inducing with 1 m m IPTG, and growing for 3 h. Harvested bacteria were stored at −70 °C.

Sequencing:

Article Title: CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli
Article Snippet: .. Following ligation, pCR2.1 and pCDF-1b-derived plasmids were transformed in TOP10 (Invitrogen) or NovaBlue (Novagen) strains, respectively, and constructions were verified by sequencing with primers T7 (pCR2.1 and pCDF-1b) and M13R (pCR2.1) or T7t (pCDF-1b). .. Detection of CRISPR, leader and PAM sequences Leaders of ECOR strains were determined by sequencing PCR amplification products as described by Diez-Villaseñor et al. CRISPR and leaders in available E. coli genomes were identified with the CRISPRFinder program ( www.crispr.u-psud.fr/Server/ ).

Transformation Assay:

Article Title: Origin of the 2-Amino-2-deoxy-gluconate Unit in Rhizobium leguminosarum Lipid A: EXPRESSION CLONING OF THE OUTER MEMBRANE OXIDASE LpxQ*
Article Snippet: .. The final recombinant plasmids were then transformed, as indicated below, into competent E. coli cells of strain BLR(DE3)/pLysS or Novablue(DE3) (Novagen) to evaluate the overexpression of LpxQ upon induction of mid-log phase cells at 37 °C with 1 mM IPTG. .. The ligation mixture that yielded pQN231 was first transformed into competent cells of E. coli HB101.

Article Title: CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli
Article Snippet: .. Following ligation, pCR2.1 and pCDF-1b-derived plasmids were transformed in TOP10 (Invitrogen) or NovaBlue (Novagen) strains, respectively, and constructions were verified by sequencing with primers T7 (pCR2.1 and pCDF-1b) and M13R (pCR2.1) or T7t (pCDF-1b). .. Detection of CRISPR, leader and PAM sequences Leaders of ECOR strains were determined by sequencing PCR amplification products as described by Diez-Villaseñor et al. CRISPR and leaders in available E. coli genomes were identified with the CRISPRFinder program ( www.crispr.u-psud.fr/Server/ ).

Article Title: Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold
Article Snippet: .. The isolated DNA was further transformed into Novablue™ (E. coli ) cells (EMD Biosciences, San Diego, CA). .. A Qiagen miniprep kit was used to isolate plasmids from E.coli (Qiagen, Valencia, CA) and sequenced using the primer 5′ ACT ACG CTC TGC AGG CTA GT 3′.

Article Title: Identification of a Novel Streptococcal Adhesin P (SadP) Protein Recognizing Galactosyl-?1-4-galactose-containing Glycoconjugates
Article Snippet: .. After cloning into pET28a and transformation into E. coli DH5α, plasmids were sequenced and were then transformed to the BL21(DE3) or Novablue(DE3) expression strains (Novagen). .. The protein was produced in E. coli by growing the bacteria to an A 550 of 0.5, inducing with 1 m m IPTG, and growing for 3 h. Harvested bacteria were stored at −70 °C.

Recombinant:

Article Title: Origin of the 2-Amino-2-deoxy-gluconate Unit in Rhizobium leguminosarum Lipid A: EXPRESSION CLONING OF THE OUTER MEMBRANE OXIDASE LpxQ*
Article Snippet: .. The final recombinant plasmids were then transformed, as indicated below, into competent E. coli cells of strain BLR(DE3)/pLysS or Novablue(DE3) (Novagen) to evaluate the overexpression of LpxQ upon induction of mid-log phase cells at 37 °C with 1 mM IPTG. .. The ligation mixture that yielded pQN231 was first transformed into competent cells of E. coli HB101.

Over Expression:

Article Title: Origin of the 2-Amino-2-deoxy-gluconate Unit in Rhizobium leguminosarum Lipid A: EXPRESSION CLONING OF THE OUTER MEMBRANE OXIDASE LpxQ*
Article Snippet: .. The final recombinant plasmids were then transformed, as indicated below, into competent E. coli cells of strain BLR(DE3)/pLysS or Novablue(DE3) (Novagen) to evaluate the overexpression of LpxQ upon induction of mid-log phase cells at 37 °C with 1 mM IPTG. .. The ligation mixture that yielded pQN231 was first transformed into competent cells of E. coli HB101.

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    Millipore novablue
    Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue/product/Millipore
    Average 89 stars, based on 2 article reviews
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    Millipore novablue de3 competent e
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