novablue singles e scherichia coli competent cells  (Millipore)


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    Structured Review

    Millipore novablue singles e scherichia coli competent cells
    Novablue Singles E Scherichia Coli Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue singles e scherichia coli competent cells/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    novablue singles e scherichia coli competent cells - by Bioz Stars, 2020-03
    80/100 stars

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    Conjugation Assay:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids. .. E. coli strain K-12, resistant to kanamycin and dibekacin and containing a chromosomal copy of the conjugation machinery from an IncP plasmid , was used for mobilization of pB1000.

    Isolation:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: All 13 strains characterized in this study were isolated in the Centro de Vigilancia Sanitaria Veterinaria laboratory ( ) from diseased pigs belonging to five different farms in different geographic areas within Spain. .. Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids.

    Cell Culture:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: .. Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids. .. E. coli strain K-12, resistant to kanamycin and dibekacin and containing a chromosomal copy of the conjugation machinery from an IncP plasmid , was used for mobilization of pB1000.

    Plasmid Preparation:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids. .. E. coli strain K-12, resistant to kanamycin and dibekacin and containing a chromosomal copy of the conjugation machinery from an IncP plasmid , was used for mobilization of pB1000.

    Polymerase Chain Reaction:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids. .. Identification of P. multocida isolates was confirmed using species-specific PCR with primers KMT1T7 and KMT1SP6 (Table ), as previously described ( ).

    Transformation Assay:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: .. Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids. .. E. coli strain K-12, resistant to kanamycin and dibekacin and containing a chromosomal copy of the conjugation machinery from an IncP plasmid , was used for mobilization of pB1000.

    Pulsed-Field Gel:

    Article Title: Multiresistance in Pasteurella multocida Is Mediated by Coexistence of Small Plasmids ▿
    Article Snippet: P. multocida ATCC 43137 served as the reference strain for pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility analysis. .. Bacteria were cultured on Columbia agar plus 5% sheep blood plates (bioMérieux, France) and in brain heart infusion (BHI) broth at 37°C for 24 h. NovaBlue Singles E scherichia coli competent cells (Novagen, Merck Chemicals Ltd., United Kingdom) and CaCl2 -treated E. coli DH5α competent cells were used in transformation of the plasmids.