novablue de3 competent e  (Millipore)


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    Structured Review

    Millipore novablue de3 competent e
    Novablue De3 Competent E, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue de3 competent e/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    novablue de3 competent e - by Bioz Stars, 2020-04
    92/100 stars

    Related Products / Commonly Used Together

    sfβgly
    pae

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    Related Articles

    Clone Assay:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: .. Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA). ..

    Centrifugation:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA). .. Cells expressing recombinant enzymes were collected by centrifugation at 4,000 × g for 20 min (4°C) and frozen at -80°C until use.

    Mutagenesis:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Paragraph title: Mutagenesis, expression in E . coli and purification of wild-type Sfβgly and its mutants ... Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA).

    Purification:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Paragraph title: Mutagenesis, expression in E . coli and purification of wild-type Sfβgly and its mutants ... Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA).

    Expressing:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Paragraph title: Mutagenesis, expression in E . coli and purification of wild-type Sfβgly and its mutants ... Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA).

    Polymerase Chain Reaction:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Mutagenesis, expression in E . coli and purification of wild-type Sfβgly and its mutants The wild-type Sfβgly gene cloned into pAE plasmid [ ] was used as template for mutagenic PCR reactions using mutagenic primers (Table A in ) and the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA), following the manufacturer’s instructions. .. Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA).

    Recombinant:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA). .. Individual colonies were used to inoculate LB broth containing 50 μg/mL ampicillin and grown to an optical density of 0.5 at 600 nm, when 0.4 mM isopropyl β-D-1-thiogalactopyranoside was added to induce the expression of recombinant wild-type and mutants of Sfβgly for 16 h at 20°C.

    Plasmid Preparation:

    Article Title: Using the Amino Acid Network to Modulate the Hydrolytic Activity of β-Glycosidases
    Article Snippet: Mutagenesis, expression in E . coli and purification of wild-type Sfβgly and its mutants The wild-type Sfβgly gene cloned into pAE plasmid [ ] was used as template for mutagenic PCR reactions using mutagenic primers (Table A in ) and the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA), following the manufacturer’s instructions. .. Wild-type or mutants of Sfβgly cloned into pAE were used to transform NovaBlue (DE3) competent E . coli cells (EMD Millipore, Billerica, MA, USA).

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  • About
  • News
  • Press Release
  • Team
  • Advisors
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  • Contact
  • Bioz Stars
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  • 92
    Millipore novablue de3 competent e
    Novablue De3 Competent E, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/novablue de3 competent e/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    novablue de3 competent e - by Bioz Stars, 2020-04
    92/100 stars
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