normal human embryonic lung fibroblasts hlf cell line  (ATCC)

Journal: OncoTargets and therapy

Article Title: Expression and prognostic value of GalNAc-T3 in patients with completely resected small (≤2 cm) peripheral lung adenocarcinoma after IASLC/ATS/ERS classification

doi: 10.2147/OTT.S93486

Figure Lengend Snippet: Human lung adenocarcinoma cell lines (A549, H322, Hcc827, and H1299) and normal human embryonic lung fibroblasts HLF cell line were cultured in complete growth media, the total mRNA and protein were extracted and tested by RT-PCR and Western blot. Notes: ( A ) and ( B ) were the result of the mRNA and protein expression of GalNAc-T3 respectively and the histogram was used as quantification of GalNAc-T3 expression in different cell lines. The expression of GalNAc-T3 was calculated relative to the β-actin expression. The data were repeated three times and are presented as the mean ± SD ( * P <0.05, ** P ≤0.001, and *** P >0.005). Abbreviations: RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: Human lung adenocarcinoma cell lines (A549, H322, Hcc827, and H1299) and normal human embryonic lung fibroblasts HLF cell line were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Standard Deviation

Journal: Frontiers in Oncology

Article Title: YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma

doi: 10.3389/fonc.2021.702491

Figure Lengend Snippet: YBX1 and MUC1 expression was the highly correlated and affected prognosis. (A) YBX1 and MUC1 mRNA expression were based on the Broet Lung and Bhattacharjee Lung datasets from the Oncomine database (p<0.05, p<0.05, respectively). (B) Western blot analysis of YBX1 and MUC1 expression in 6 pairs of lung adenocarcinoma (T) and adjacent tissues (N) (r = 0.5864, P < 0.0037, Red dot: T, Black dot: N). (C) Western blot analysis of YBX1 and MUC1 expression in HLF and 6 lung adenocarcinoma cell lines (r = 0.8789, P < 0.0057). (D) RT-PCR analysis of YBX1 and MUC1 expression in HLF and lung adenocarcinoma cell lines (r = 0.9306, P < 0.0018). (E) IHC analysis of YBX1 and MUC1 expression in lung adenocarcinoma. Original magnification 200× and 400× in the inset. (F) YBX1 and MUC1 expression was positive correlated in 176 lung adenocarcinoma specimens (r = 0.357, P < 0.001). (G) The OS curves of YBX1/MUC1 expression in 176 patients with lung adenocarcinoma (p <0.001). (H) The DFS curves of YBX1/MUC1 expression in 176 patients (p <0.001). The data are presented as mean ± SD of three independent tests.

Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299, NCI-H322, HCC827, and NCI-H358), one normal human embryonic lung fibroblast cell line (HLF), and 293T cells were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: GED-0507 attenuates lung fibrosis by counteracting myofibroblast transdifferentiation in vivo and in vitro

doi: 10.1371/journal.pone.0257281

Figure Lengend Snippet: Experimental plan. Myofibroblast activation was induced in primary human fibroblasts (HLFs) stimulated with 5 ng/mL TGFβ over two days. GED-0507 (1–30 mM), Pirf (0.1–0.4 mM) or Nint (0.1–0.5 μM) were administered at two different doses at the same time of TGFβ. mRNA expression levels for (a) TGFB1 , (b) ACTA , (d) COL1A1 and (f) FN1 were quantified in RT-PCRs and converted into histograms for 3 independent experiments performed in triplicate. Protein expression levels for (c) α-SMA, (e) collagen and (g) fibronectin were evaluated on Western blots. Band density was quantified using ImageJ software and converted into histograms for 3 independent experiments in duplicate. Since Pirf and Nint were solubilized in DMSO, DMSO- and/or TGFβ+DMSO-treated cells were used as comparators in the statistical analysis. Data are quoted as the mean ± SEM fold change vs. CTRL cells. * = p< 0.05; ** = p< 0.01; *** = p< 0.001.

Article Snippet: HLFs (ATCC® PCS-201-013™, Homo Sapiens , Normal Lung) were grown in Fibroblast Basal Medium (ATCC® PCS-201-030™) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin and a Fibroblast Growth Kit-Low serum (ATCC® PCS-201-041™), according to the manufacturer’s instructions.

Techniques: Activation Assay, Expressing, Western Blot, Software