normal human embryonic lung fibroblasts hlf cell line  (ATCC)

Journal: OncoTargets and therapy

Article Title: Expression and prognostic value of GalNAc-T3 in patients with completely resected small (≤2 cm) peripheral lung adenocarcinoma after IASLC/ATS/ERS classification

doi: 10.2147/OTT.S93486

Figure Lengend Snippet: Human lung adenocarcinoma cell lines (A549, H322, Hcc827, and H1299) and normal human embryonic lung fibroblasts HLF cell line were cultured in complete growth media, the total mRNA and protein were extracted and tested by RT-PCR and Western blot. Notes: ( A ) and ( B ) were the result of the mRNA and protein expression of GalNAc-T3 respectively and the histogram was used as quantification of GalNAc-T3 expression in different cell lines. The expression of GalNAc-T3 was calculated relative to the β-actin expression. The data were repeated three times and are presented as the mean ± SD ( * P <0.05, ** P ≤0.001, and *** P >0.005). Abbreviations: RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: Human lung adenocarcinoma cell lines (A549, H322, Hcc827, and H1299) and normal human embryonic lung fibroblasts HLF cell line were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Standard Deviation

Journal: Frontiers in Oncology

Article Title: YBX1 Enhances Metastasis and Stemness by Transcriptionally Regulating MUC1 in Lung Adenocarcinoma

doi: 10.3389/fonc.2021.702491

Figure Lengend Snippet: YBX1 and MUC1 expression was the highly correlated and affected prognosis. (A) YBX1 and MUC1 mRNA expression were based on the Broet Lung and Bhattacharjee Lung datasets from the Oncomine database (p<0.05, p<0.05, respectively). (B) Western blot analysis of YBX1 and MUC1 expression in 6 pairs of lung adenocarcinoma (T) and adjacent tissues (N) (r = 0.5864, P < 0.0037, Red dot: T, Black dot: N). (C) Western blot analysis of YBX1 and MUC1 expression in HLF and 6 lung adenocarcinoma cell lines (r = 0.8789, P < 0.0057). (D) RT-PCR analysis of YBX1 and MUC1 expression in HLF and lung adenocarcinoma cell lines (r = 0.9306, P < 0.0018). (E) IHC analysis of YBX1 and MUC1 expression in lung adenocarcinoma. Original magnification 200× and 400× in the inset. (F) YBX1 and MUC1 expression was positive correlated in 176 lung adenocarcinoma specimens (r = 0.357, P < 0.001). (G) The OS curves of YBX1/MUC1 expression in 176 patients with lung adenocarcinoma (p <0.001). (H) The DFS curves of YBX1/MUC1 expression in 176 patients (p <0.001). The data are presented as mean ± SD of three independent tests.

Article Snippet: Human lung adenocarcinoma cell lines (A549, NCI-H1299, NCI-H322, HCC827, and NCI-H358), one normal human embryonic lung fibroblast cell line (HLF), and 293T cells were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction

Journal: Oncotarget

Article Title: YBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma

doi: 10.18632/oncotarget.10080

Figure Lengend Snippet: A. Co-localization of CDC25a and YBX1 in HLF, A549, H322 and Hcc827 cells by IF assay. Red: YBX1; Green: CDC25a. B,C,D. The expression of CDC25a and YBX1 proteins in total (W), nuclear (N) and cytoplasm (C) protein in lung adenocarcinoma cell lines and HLF cells detected by Western Blot. Expression of CDC25a and YBX1 were calculated relative to the Beta-actin or Histone H3 expression. The quantification of YBX1 and CDC25a was originated from all bands shown in the photograph. The date was repeated three times and presented as the mean±SD (*p<0.05, **p=<0.001 and # p>0.005).

Article Snippet: Three human lung adenocarcinoma cell lines (A549, H322 and Hcc827) and one normal human embryonic lung fibroblasts cell line (HLF) were obtained from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Expressing, Western Blot

Journal: Oncotarget

Article Title: YBX1 regulates tumor growth via CDC25a pathway in human lung adenocarcinoma

doi: 10.18632/oncotarget.10080

Figure Lengend Snippet: A. In accordance with luciferase activity, the ChIP assays were performed in HLF, A549 and H322 cells to confirm the endogenous of YBX1 binding to the CDC25a promoter (−352 to −153 bp) using an anti-YB-1 antibodyor normal rabbit IgG. B. YBX1 knockdown reduced endogenous YBX1 binding to the CDC25a promoter using ChIP assays in A549 and H322 cells. C. RT-PCR assay revealed si-YBX1 inhibited the mRNA expression of YBX1 and CDC25a in A549 and H322 cells. D. G1/S checkpoint pathway was detected by Western blot assay in A549 cells transfected with si-YBX1. E. Thesi-YBX1-transfected A549 and H322 cells stagnated in G0/G1 phase by cell cycle analysis.

Article Snippet: Three human lung adenocarcinoma cell lines (A549, H322 and Hcc827) and one normal human embryonic lung fibroblasts cell line (HLF) were obtained from American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Luciferase, Activity Assay, Binding Assay, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Transfection, Cell Cycle Assay