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Roche nonidet p40 np40
Nonidet P40 Np40, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nonidet p40 np40/product/Roche
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
nonidet p40 np40 - by Bioz Stars, 2020-04
93/100 stars

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Co-Immunoprecipitation Assay:

Article Title: Tumor necrosis factor ? treatment of HepG2 cells mobilizes a cytoplasmic pool of ERp57/1,25D3-MARRS to the nucleus
Article Snippet: Paragraph title: Co-IP of NF-κB and ERp57 in HepG2 cells following treatment ... The 50 mM NEM PBS solution was removed and 0.75 ml cold 0.5% (v/v) Nonidet P40 (NP40) (Roche, cat# 11-754-599-001) in PBS with 10 mM NEM, 1/100 protease inhibitor cocktail (PIC) (Sigma, cat# P8340), and 1/100 phosphatase inhibitor cocktail (Calbiochem, Madison, Wisconsin, cat# 524625) was added, the cells were then scraped loose with a cell scraper, and incubated on ice for 20 min. Lysates for each time point or condition (e.g. 15 min TNF-α or vehicle control treatment) were collected in a microcentrifuge tube and centrifuged at 10,000×g at 4°C for 15 min to pellet any cell debris.

Immunoprecipitation:

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: Paragraph title: Immunoprecipitation ... Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ).

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: .. Immunoprecipitation Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). ..

Protease Inhibitor:

Article Title: Tumor necrosis factor ? treatment of HepG2 cells mobilizes a cytoplasmic pool of ERp57/1,25D3-MARRS to the nucleus
Article Snippet: .. The 50 mM NEM PBS solution was removed and 0.75 ml cold 0.5% (v/v) Nonidet P40 (NP40) (Roche, cat# 11-754-599-001) in PBS with 10 mM NEM, 1/100 protease inhibitor cocktail (PIC) (Sigma, cat# P8340), and 1/100 phosphatase inhibitor cocktail (Calbiochem, Madison, Wisconsin, cat# 524625) was added, the cells were then scraped loose with a cell scraper, and incubated on ice for 20 min. Lysates for each time point or condition (e.g. 15 min TNF-α or vehicle control treatment) were collected in a microcentrifuge tube and centrifuged at 10,000×g at 4°C for 15 min to pellet any cell debris. .. The supernatant was equally divided into separate centrifuge tubes: one for incubation in 2 μg/ml non-immune ChromPur Rabbit IgG (Jackson ImmunoResearch, cat# 011-000-003), one for incubation in 2 μg/ml p65 (NF-κB) Ab (SantaCruz Biotech, SC-109), and, in some experiments, one for incubation with 2 μg/ml of a rabbit polyclonal Ab specific for the C-terminus of ERp57 (Affinity BioReagents, cat# PA3-009).

Article Title: Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
Article Snippet: .. Apoptotic and non-apoptotic Jurkat cells were resuspended in Nonidet P40 (NP40)-containing lysis buffer (25 mM Tris-HCl, pH 7.6, 100 mM KCl, 10 mM MgCl2 , 0.25 mM dithioerythritol, 1% NP40, Complete™ protease inhibitor cocktail [Roche]) at a concentration of 108 cells/ml. .. SDS–polyacrylamide gel electrophoresis and western blotting Cell extracts of 1.3 × 107 non-apoptotic Jurkat cells and 1.3 × 107 apoptotic Jurkat cells, either separately or mixed, were separated by SDS–polyacrylamide gel electrophoresis.

Centrifugation:

Article Title: Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
Article Snippet: Eight hours after induction, apoptotic cells were harvested by centrifugation at 800 g for 10 min and washed with PBS. .. Apoptotic and non-apoptotic Jurkat cells were resuspended in Nonidet P40 (NP40)-containing lysis buffer (25 mM Tris-HCl, pH 7.6, 100 mM KCl, 10 mM MgCl2 , 0.25 mM dithioerythritol, 1% NP40, Complete™ protease inhibitor cocktail [Roche]) at a concentration of 108 cells/ml.

Cell Culture:

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: .. Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). ..

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: .. Immunoprecipitation Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). ..

Concentration Assay:

Article Title: Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
Article Snippet: .. Apoptotic and non-apoptotic Jurkat cells were resuspended in Nonidet P40 (NP40)-containing lysis buffer (25 mM Tris-HCl, pH 7.6, 100 mM KCl, 10 mM MgCl2 , 0.25 mM dithioerythritol, 1% NP40, Complete™ protease inhibitor cocktail [Roche]) at a concentration of 108 cells/ml. .. SDS–polyacrylamide gel electrophoresis and western blotting Cell extracts of 1.3 × 107 non-apoptotic Jurkat cells and 1.3 × 107 apoptotic Jurkat cells, either separately or mixed, were separated by SDS–polyacrylamide gel electrophoresis.

Incubation:

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). .. For immunoprecipitation of SFB-tagged proteins, 30 μl S-protein agarose beads (69704; MilliporeSigma) were washed twice with NP40 lysis buffer and incubated with the cell lysate overnight at 4°C.

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: Immunoprecipitation Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). .. For immunoprecipitation of SFB-tagged proteins, 30 μl S-protein agarose beads (69704; MilliporeSigma) were washed twice with NP40 lysis buffer and incubated with the cell lysate overnight at 4°C.

Article Title: Tumor necrosis factor ? treatment of HepG2 cells mobilizes a cytoplasmic pool of ERp57/1,25D3-MARRS to the nucleus
Article Snippet: .. The 50 mM NEM PBS solution was removed and 0.75 ml cold 0.5% (v/v) Nonidet P40 (NP40) (Roche, cat# 11-754-599-001) in PBS with 10 mM NEM, 1/100 protease inhibitor cocktail (PIC) (Sigma, cat# P8340), and 1/100 phosphatase inhibitor cocktail (Calbiochem, Madison, Wisconsin, cat# 524625) was added, the cells were then scraped loose with a cell scraper, and incubated on ice for 20 min. Lysates for each time point or condition (e.g. 15 min TNF-α or vehicle control treatment) were collected in a microcentrifuge tube and centrifuged at 10,000×g at 4°C for 15 min to pellet any cell debris. .. The supernatant was equally divided into separate centrifuge tubes: one for incubation in 2 μg/ml non-immune ChromPur Rabbit IgG (Jackson ImmunoResearch, cat# 011-000-003), one for incubation in 2 μg/ml p65 (NF-κB) Ab (SantaCruz Biotech, SC-109), and, in some experiments, one for incubation with 2 μg/ml of a rabbit polyclonal Ab specific for the C-terminus of ERp57 (Affinity BioReagents, cat# PA3-009).

Lysis:

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: .. Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). ..

Article Title: STIM2 interacts with AMPK and regulates calcium-induced AMPK activation
Article Snippet: .. Immunoprecipitation Cultured cells were washed with PBS and lysed using NP40 lysis buffer [50 mM Tris (pH 7.4), 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 mM Na3 VO4 , 1% Nonidet P40 (NP40) (pH 8.0)] containing complete mini protease inhibitors (11836153001; Roche, Basel, Switzerland) and phosphatase inhibitor cocktail (524625; Calbiochem, San Diego, CA, USA) as previously described ( ). ..

Article Title: Autoantibodies specific for apoptotic U1-70K are superior serological markers for mixed connective tissue disease
Article Snippet: .. Apoptotic and non-apoptotic Jurkat cells were resuspended in Nonidet P40 (NP40)-containing lysis buffer (25 mM Tris-HCl, pH 7.6, 100 mM KCl, 10 mM MgCl2 , 0.25 mM dithioerythritol, 1% NP40, Complete™ protease inhibitor cocktail [Roche]) at a concentration of 108 cells/ml. .. SDS–polyacrylamide gel electrophoresis and western blotting Cell extracts of 1.3 × 107 non-apoptotic Jurkat cells and 1.3 × 107 apoptotic Jurkat cells, either separately or mixed, were separated by SDS–polyacrylamide gel electrophoresis.

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