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Roche nonidet p40 detergent
Nonidet P40 Detergent, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nonidet p40 detergent/product/Roche
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
nonidet p40 detergent - by Bioz Stars, 2020-04
91/100 stars

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Binding Assay:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: GTP binding assay GTP-γ35 S binding was measured in a 96 well format using a modified antibody capture technique previously described [ , ]. .. This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation.

Scintillation Proximity Assay:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation. .. 1.25 mg of anti-rabbit antibody scintillation proximity assay bead (GE Healthcare) was then added, and plates were incubated for an additional hour.

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: .. A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells. ..

Potency Assay:

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: Paragraph title: CB1 and CB2 GTPγ35 S potency assay ... A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells.

Concentration Assay:

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells. .. Antagonist K b values (potency) were calculated with a modification of the Cheng-Prusoff relationship as follows: K b = IC50 /[1 + [Agonist]/EC50 ], where IC50 is determined from a four parameter fit of displacement curves, [agonist] = EC50 of full agonist, and EC50 is determined from a four parameter fit of a full agonist concentration response curve.

Incubation:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: .. This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation. .. 1.25 mg of anti-rabbit antibody scintillation proximity assay bead (GE Healthcare) was then added, and plates were incubated for an additional hour.

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: GTPγ35 S potency of 3 , 8 , 16 , 20 and 22 in CHO (Lonza, Walkersville, MD) or Sf9 (PerkinElmer Life Sciences, Boston, MA) cell membranes that ectopically express human CB1 or CB2 receptors, respectively, was measured in a 96-well format with a modified antibody capture technique as described previously.25,41 The following exceptions applied to the CB1 CHO GTPγ35 S assays: membranes (1 unit/well), compound, and GTPγ35 S (500 pM) were incubated in GTP-binding assay buffer (20 mM HEPES, 100 mM NaCl, 5 mM MgCl2 , 0.5% fatty acid free bovine serum albumin (Serologicals Corp, Norcross, GA), 1 μM GDP, pH 7.4) at room temperature for 30 min. Antagonist dose responses were performed in the presence of an 80% efficacious dose of full agonist (methanandamide). .. A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells.

Modification:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: GTP binding assay GTP-γ35 S binding was measured in a 96 well format using a modified antibody capture technique previously described [ , ]. .. This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation.

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: GTPγ35 S potency of 3 , 8 , 16 , 20 and 22 in CHO (Lonza, Walkersville, MD) or Sf9 (PerkinElmer Life Sciences, Boston, MA) cell membranes that ectopically express human CB1 or CB2 receptors, respectively, was measured in a 96-well format with a modified antibody capture technique as described previously.25,41 The following exceptions applied to the CB1 CHO GTPγ35 S assays: membranes (1 unit/well), compound, and GTPγ35 S (500 pM) were incubated in GTP-binding assay buffer (20 mM HEPES, 100 mM NaCl, 5 mM MgCl2 , 0.5% fatty acid free bovine serum albumin (Serologicals Corp, Norcross, GA), 1 μM GDP, pH 7.4) at room temperature for 30 min. Antagonist dose responses were performed in the presence of an 80% efficacious dose of full agonist (methanandamide). .. A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells.

Gradient Centrifugation:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: CEM membrane prepared by sucrose density gradient centrifugation, SDF-1α, and compounds were combined and incubated for 15 minutes at room temperature. .. This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation.

GTP Binding Assay:

Article Title: Inhibition of CXCR4 by LY2624587, a Fully Humanized Anti-CXCR4 Antibody Induces Apoptosis of Hematologic Malignancies
Article Snippet: Paragraph title: GTP binding assay ... This was followed by the addition of 0.27% Nonidet P40 detergent (Roche) and anti-Gi antibody (final dilution of 1:400; Covance) each followed by a 15 minute incubation.

Article Title: Synthesis, Ex Vivo Evaluation and Radiolabeling of Potent 1,5-Diphenyl-pyrrolidin-2-one Cannabinoid Subtype-1 (CB1) Receptor Ligands as Candidates for In Vivo Imaging
Article Snippet: GTPγ35 S potency of 3 , 8 , 16 , 20 and 22 in CHO (Lonza, Walkersville, MD) or Sf9 (PerkinElmer Life Sciences, Boston, MA) cell membranes that ectopically express human CB1 or CB2 receptors, respectively, was measured in a 96-well format with a modified antibody capture technique as described previously.25,41 The following exceptions applied to the CB1 CHO GTPγ35 S assays: membranes (1 unit/well), compound, and GTPγ35 S (500 pM) were incubated in GTP-binding assay buffer (20 mM HEPES, 100 mM NaCl, 5 mM MgCl2 , 0.5% fatty acid free bovine serum albumin (Serologicals Corp, Norcross, GA), 1 μM GDP, pH 7.4) at room temperature for 30 min. Antagonist dose responses were performed in the presence of an 80% efficacious dose of full agonist (methanandamide). .. A mixture containing 0.2% Nonidet P40 detergent (Roche, Indianapolis, IN), anti-Gi antibody (final dilution of 1:362; Covance, Princeton, NJ), and 1.25 mg anti-rabbit antibody scintillation proximity assay beads (GE Healthcare, Piscataway, NJ) was added to all wells.

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  • 96
    Roche non ionic detergent buffer
    Non Ionic Detergent Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non ionic detergent buffer/product/Roche
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
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    85
    Roche detergent nonidet p40 np40
    Detergent Nonidet P40 Np40, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/detergent nonidet p40 np40/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    detergent nonidet p40 np40 - by Bioz Stars, 2020-04
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    91
    Roche nonidet p40 detergent
    Nonidet P40 Detergent, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nonidet p40 detergent/product/Roche
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nonidet p40 detergent - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

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