Structured Review

Beyotime nonidet p 40
Nonidet P 40, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nonidet p 40/product/Beyotime
Average 99 stars, based on 26 article reviews
Price from $9.99 to $1999.99
nonidet p 40 - by Bioz Stars, 2020-11
99/100 stars

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Transfection:

Article Title: MYH9 Aggregation Induced by Direct Interaction With PRRSV GP5 Ectodomain Facilitates Viral Internalization by Permissive Cells
Article Snippet: .. At 48 h after transfection, cells were lysed using NP-40 cell lysis buffer (Beyotime, Jiangsu, China) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and clarified as described above. .. Next, Co-IP was conducted using anti-HA monoclonal antibody (Sigma-Aldrich) following the same protocol described above.

Protease Inhibitor:

Article Title: Transferrin receptor 1 levels at the cell surface influence the susceptibility of newborn piglets to PEDV infection
Article Snippet: .. After 24 h, cells were lysed in NP-40 lysis buffer containing protease inhibitor cocktail then centrifuged at 12000×g for 10 min. .. The supernatants were pretreated with protein A/G magnetic beads and IgG (from the same species as the immunoprecipitating antibody) for 1 h at 4°C to eliminate nonspecific binding to the beads.

Article Title: MYH9 Aggregation Induced by Direct Interaction With PRRSV GP5 Ectodomain Facilitates Viral Internalization by Permissive Cells
Article Snippet: .. At 48 h after transfection, cells were lysed using NP-40 cell lysis buffer (Beyotime, Jiangsu, China) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and clarified as described above. .. Next, Co-IP was conducted using anti-HA monoclonal antibody (Sigma-Aldrich) following the same protocol described above.

Article Title: Porcine RING Finger Protein 114 Inhibits Classical Swine Fever Virus Replication via K27-Linked Polyubiquitination of Viral NS4B
Article Snippet: .. Samples were lysed with NP-40 lysis buffer containing a protease inhibitor (1 mM phenylmethylsulfonyl fluoride [PMSF]; Beyotime) for 30 min and then centrifuged for 20 min at 4°C; the supernatants were denatured in 5× sample loading buffer for 7 min at 100°C. .. For immunoprecipitation assays, the supernatants were mixed with the respective primary antibodies in the presence of protein G-agarose (Roche) at 4°C overnight, followed by Western blotting.

Centrifugation:

Article Title: Particularly interesting Cys-His-rich protein is highly expressed in human intracranial aneurysms and resists aneurysmal rupture
Article Snippet: .. Western blot analysis Tumor tissues were homogenized and NP-40 buffer (P0013F; Beyotime Institute of Biotechnology) was used to extract them, followed by 5–10 min of boiling and centrifugation at 7,200 × g for 15 min at 4°C in order to obtain the supernatant. .. A 10% SDS-PAGE gel was used to separate samples containing 50 µg protein, which were then transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Cell Culture:

Article Title: Transplantation of adipose-derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus-induced erectile dysfunction in rats
Article Snippet: .. Western blot analysis Protein extraction from rat penile tissues and cultured cells was performed using NP-40 lysis buffer (Beyotime, Nantong, China). .. Protein concentration was detected with the BCA protein assay kit (Beyotime, Nantong, China).

Concentration Assay:

Article Title: Interferon-Inducible Oligoadenylate Synthetase-Like Protein Acts as an Antiviral Effector against Classical Swine Fever Virus via the MDA5-Mediated Type I Interferon-Signaling Pathway
Article Snippet: .. At 48 hpt, the cells were lysed with NP-40 lysis buffer (catalog no. P0013F; Beyotime) containing phenylmethanesulfonyl fluoride (PMSF) to a final concentration of 1 mM for 1 h at 4°C. .. To remove cellular debris, the lysates were centrifuged at 12,000 × g at 4°C for 25 min, and protein G-agarose (catalog no. 11243233001; Roche) was used to preclear the supernatants for 4 h at 4°C and then incubated with an anti-Flag MAb (catalog no. F1804; Sigma-Aldrich) or with an irrelevant isotype-matched MAb (catalog no. A00185; GeneScript) acting as a negative control for 5 h at 4°C.

Protein Extraction:

Article Title: Transplantation of adipose-derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus-induced erectile dysfunction in rats
Article Snippet: .. Western blot analysis Protein extraction from rat penile tissues and cultured cells was performed using NP-40 lysis buffer (Beyotime, Nantong, China). .. Protein concentration was detected with the BCA protein assay kit (Beyotime, Nantong, China).

Lysis:

Article Title: Transferrin receptor 1 levels at the cell surface influence the susceptibility of newborn piglets to PEDV infection
Article Snippet: .. After 24 h, cells were lysed in NP-40 lysis buffer containing protease inhibitor cocktail then centrifuged at 12000×g for 10 min. .. The supernatants were pretreated with protein A/G magnetic beads and IgG (from the same species as the immunoprecipitating antibody) for 1 h at 4°C to eliminate nonspecific binding to the beads.

Article Title: Transplantation of adipose-derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus-induced erectile dysfunction in rats
Article Snippet: .. Western blot analysis Protein extraction from rat penile tissues and cultured cells was performed using NP-40 lysis buffer (Beyotime, Nantong, China). .. Protein concentration was detected with the BCA protein assay kit (Beyotime, Nantong, China).

Article Title: Interferon-Inducible Oligoadenylate Synthetase-Like Protein Acts as an Antiviral Effector against Classical Swine Fever Virus via the MDA5-Mediated Type I Interferon-Signaling Pathway
Article Snippet: .. At 48 hpt, the cells were lysed with NP-40 lysis buffer (catalog no. P0013F; Beyotime) containing phenylmethanesulfonyl fluoride (PMSF) to a final concentration of 1 mM for 1 h at 4°C. .. To remove cellular debris, the lysates were centrifuged at 12,000 × g at 4°C for 25 min, and protein G-agarose (catalog no. 11243233001; Roche) was used to preclear the supernatants for 4 h at 4°C and then incubated with an anti-Flag MAb (catalog no. F1804; Sigma-Aldrich) or with an irrelevant isotype-matched MAb (catalog no. A00185; GeneScript) acting as a negative control for 5 h at 4°C.

Article Title: MYH9 Aggregation Induced by Direct Interaction With PRRSV GP5 Ectodomain Facilitates Viral Internalization by Permissive Cells
Article Snippet: .. At 48 h after transfection, cells were lysed using NP-40 cell lysis buffer (Beyotime, Jiangsu, China) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) and clarified as described above. .. Next, Co-IP was conducted using anti-HA monoclonal antibody (Sigma-Aldrich) following the same protocol described above.

Article Title: Porcine RING Finger Protein 114 Inhibits Classical Swine Fever Virus Replication via K27-Linked Polyubiquitination of Viral NS4B
Article Snippet: .. Samples were lysed with NP-40 lysis buffer containing a protease inhibitor (1 mM phenylmethylsulfonyl fluoride [PMSF]; Beyotime) for 30 min and then centrifuged for 20 min at 4°C; the supernatants were denatured in 5× sample loading buffer for 7 min at 100°C. .. For immunoprecipitation assays, the supernatants were mixed with the respective primary antibodies in the presence of protein G-agarose (Roche) at 4°C overnight, followed by Western blotting.

Article Title: DDX39 Overexpression Predicts a Poor Prognosis and Promotes Aggressiveness of Melanoma by Cooperating With SNAIL
Article Snippet: .. Western Blot AnalysisTotal protein of the cell lysate in each group was extracted using NP-40 lysis buffer (Beyotime Biotechnology, Shanghai, China). .. The protein concentration was determined using a bicinchoninic acid protein concentration quantification kit (Beyotime Biotechnology).

Western Blot:

Article Title: Transplantation of adipose-derived stem cells overexpressing inducible nitric oxide synthase ameliorates diabetes mellitus-induced erectile dysfunction in rats
Article Snippet: .. Western blot analysis Protein extraction from rat penile tissues and cultured cells was performed using NP-40 lysis buffer (Beyotime, Nantong, China). .. Protein concentration was detected with the BCA protein assay kit (Beyotime, Nantong, China).

Article Title: Particularly interesting Cys-His-rich protein is highly expressed in human intracranial aneurysms and resists aneurysmal rupture
Article Snippet: .. Western blot analysis Tumor tissues were homogenized and NP-40 buffer (P0013F; Beyotime Institute of Biotechnology) was used to extract them, followed by 5–10 min of boiling and centrifugation at 7,200 × g for 15 min at 4°C in order to obtain the supernatant. .. A 10% SDS-PAGE gel was used to separate samples containing 50 µg protein, which were then transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Article Title: DDX39 Overexpression Predicts a Poor Prognosis and Promotes Aggressiveness of Melanoma by Cooperating With SNAIL
Article Snippet: .. Western Blot AnalysisTotal protein of the cell lysate in each group was extracted using NP-40 lysis buffer (Beyotime Biotechnology, Shanghai, China). .. The protein concentration was determined using a bicinchoninic acid protein concentration quantification kit (Beyotime Biotechnology).

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  • 93
    Beyotime nonidet p 40
    Nonidet P 40, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nonidet p 40/product/Beyotime
    Average 93 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    nonidet p 40 - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    94
    Beyotime lysis buffer
    Lysis Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 94/100, based on 1527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer/product/Beyotime
    Average 94 stars, based on 1527 article reviews
    Price from $9.99 to $1999.99
    lysis buffer - by Bioz Stars, 2020-11
    94/100 stars
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