nonidet p 40  (Beyotime)

 
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    Name:
    IGEPAL CA 630
    Description:

    Catalog Number:
    ST2045-100ML
    Price:
    136.0
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    Structured Review

    Beyotime nonidet p 40

    https://www.bioz.com/result/nonidet p 40/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nonidet p 40 - by Bioz Stars, 2021-05
    86/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology
    Article Snippet: .. Enhanced Cell Counting Kit-8 (CCK-8), ACK Lysis Buffer and 1% Nonidet P-40 (NP-40) were obtained from the Beyotime Institute of Biotechnology (Jiangsu, China). ..

    Lysis:

    Article Title: Immunomodulatory effects of a new whole ingredients extract from Astragalus: a combined evaluation on chemistry and pharmacology
    Article Snippet: .. Enhanced Cell Counting Kit-8 (CCK-8), ACK Lysis Buffer and 1% Nonidet P-40 (NP-40) were obtained from the Beyotime Institute of Biotechnology (Jiangsu, China). ..

    Article Title: Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy
    Article Snippet: .. Western blot analysisThe total protein from mouse tissues were extracted using RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Beyotime, Shanghai, China) containing a freshly added proteinase inhibitor cocktail (Roche Diagnostics, Berlin, Germany). .. Protein concentrations were determined using a BCA protein assay reagent (Beyotime, Shanghai, China; P0010).

    Protease Inhibitor:

    Article Title: The oncogenic role of TRIP13 in regulating proliferation, invasion, and cell cycle checkpoint in NSCLC cells
    Article Snippet: .. Cells and tissues were lysed in RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF and protease inhibitor cocktail) and measured by BCA protein assay kit (Beyotime, Shanghai, China). .. Equal amounts of protein were separated by SDS-PAGE, and trans-blotted onto PVDF membrane by semi-dry transfer.

    Article Title: Cleavage of a pathogen apoplastic protein by plant subtilases activates immunity
    Article Snippet: Extraction of the recombinant protein from plant tissues N. benthamiana leaves expressing the recombinant proteins were ground to powder in liquid nitrogen. .. The powder was added to an extraction buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl), 10mM dithiothreitol (DTT), protease inhibitor cocktail (1:100, Sigma) and 0.15% NP-40 (Beyotime Biotechnology). .. Samples were centrifuged at max speed for 10 min at 4°C, and the supernatant was used for SDS-PAGE gel electrophoresis.

    BIA-KA:

    Article Title: The oncogenic role of TRIP13 in regulating proliferation, invasion, and cell cycle checkpoint in NSCLC cells
    Article Snippet: .. Cells and tissues were lysed in RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 1 mM PMSF and protease inhibitor cocktail) and measured by BCA protein assay kit (Beyotime, Shanghai, China). .. Equal amounts of protein were separated by SDS-PAGE, and trans-blotted onto PVDF membrane by semi-dry transfer.

    Western Blot:

    Article Title: Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy
    Article Snippet: .. Western blot analysisThe total protein from mouse tissues were extracted using RIPA lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, Beyotime, Shanghai, China) containing a freshly added proteinase inhibitor cocktail (Roche Diagnostics, Berlin, Germany). .. Protein concentrations were determined using a BCA protein assay reagent (Beyotime, Shanghai, China; P0010).

    Enzyme-linked Immunosorbent Assay:

    Article Title: The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation
    Article Snippet: Antibodies and reagents The antibodies used in this study were as follows: anti-GFP (11814460001), HRP-conjugated anti-HA (12013819001), Myc (11814150001) antibodies (Roche); phosphorylated TBK1(5483), IκBα (9246), IRF3 (4961) antibodies (Cell Signaling Technology); anti-GST-Tag (M20007L) and HRP-conjugated goat anti-mouse secondary antibody (M21001L) (Abmart); anti-His (TA-02), HRP-conjugated goat anti-rabbit IgG (ZB-2301), Alexa Fluor 488-conjugated anti-mouse IgG (ZF-0512), Alexa Fluor 594-conjugated goat anti-rabbit IgG (ZF-0516) secondary antibodies (ZSGB-BIO); anti-NBR1 (16004-1-AP), anti-TRAF3 (18099-1-AP) and anti-NDP52 (12229-1-AP) antibodies (Proteinteck); anti-MAVS antibody (BETHYL, A300-782A); anti-RNF5 (ab128200), anti-GAPDH (ab181602), anti-ubiquitin (K63) (ab179434), anti-ubiquitin(K48) (ab140601), anti-ubiquitin(K27) (ab181537) antibodies (Abcam); anti-ubiquitin (WT) antibody (Santa Cruz, sc-8017); anti-LC3(PM036) and anti-ATG7 (PM039) antibodies (MBL); anti-LAMP1 (11215-R107) and anti-NP (11675-MM03T) antibodies (Sino Biological); anti-p62/SQSTM1 (P0067) and HRP-conjugated anti-Flag (A8592) antibodies (Sigma); Cy3-labeled goat anti-mouse IgG (A0521), Dylight 405-conjugated goat anti-rabbit IgG (A0605) and Dylight 405-conjugated goat anti-mouse IgG (A0609) secondary antibodies (Beyotime); and mouse anti-PB1 monoclonal antibody (prepared in our laboratory). .. Reagents used in the study included: poly(dA:dT) (tlrl-patc) and poly(I:C) (tlrl-picwlv) (InvivoGen); a human IFN-β DuoSet ELISA kit (R & D, DY814-05); 3-Methyladenine (M9281), MG132 (M7449), DMSO (D2650), CQ (Chloroquine phosphate) (PHR1258), NH4Cl (09718), Earle’s balanced salt solution (EBSS), anti-Flag agarose affinity beads (A2220) and protein A/G agarose affinity beads (P6486/E3403) (Sigma); Glutathione Sepharose 4B (GE Healthcare, 17-0756-05); Bafilomycin A1(Baf-A1) (Selleck, S1314); DAPI (C1005), ZVAD (Caspase inhibitor Z-VAD-FMK, C1202), NP-40 (ST366), and recombinant Human IFN-β (P5660) (Beyotime). .. Human RNF5, ULK1, ATG13, FIP200, and ATG101 specific siRNAs were designed and synthesized by RiboBio Co. Ltd, China.

    Recombinant:

    Article Title: The PB1 protein of influenza A virus inhibits the innate immune response by targeting MAVS for NBR1-mediated selective autophagic degradation
    Article Snippet: Antibodies and reagents The antibodies used in this study were as follows: anti-GFP (11814460001), HRP-conjugated anti-HA (12013819001), Myc (11814150001) antibodies (Roche); phosphorylated TBK1(5483), IκBα (9246), IRF3 (4961) antibodies (Cell Signaling Technology); anti-GST-Tag (M20007L) and HRP-conjugated goat anti-mouse secondary antibody (M21001L) (Abmart); anti-His (TA-02), HRP-conjugated goat anti-rabbit IgG (ZB-2301), Alexa Fluor 488-conjugated anti-mouse IgG (ZF-0512), Alexa Fluor 594-conjugated goat anti-rabbit IgG (ZF-0516) secondary antibodies (ZSGB-BIO); anti-NBR1 (16004-1-AP), anti-TRAF3 (18099-1-AP) and anti-NDP52 (12229-1-AP) antibodies (Proteinteck); anti-MAVS antibody (BETHYL, A300-782A); anti-RNF5 (ab128200), anti-GAPDH (ab181602), anti-ubiquitin (K63) (ab179434), anti-ubiquitin(K48) (ab140601), anti-ubiquitin(K27) (ab181537) antibodies (Abcam); anti-ubiquitin (WT) antibody (Santa Cruz, sc-8017); anti-LC3(PM036) and anti-ATG7 (PM039) antibodies (MBL); anti-LAMP1 (11215-R107) and anti-NP (11675-MM03T) antibodies (Sino Biological); anti-p62/SQSTM1 (P0067) and HRP-conjugated anti-Flag (A8592) antibodies (Sigma); Cy3-labeled goat anti-mouse IgG (A0521), Dylight 405-conjugated goat anti-rabbit IgG (A0605) and Dylight 405-conjugated goat anti-mouse IgG (A0609) secondary antibodies (Beyotime); and mouse anti-PB1 monoclonal antibody (prepared in our laboratory). .. Reagents used in the study included: poly(dA:dT) (tlrl-patc) and poly(I:C) (tlrl-picwlv) (InvivoGen); a human IFN-β DuoSet ELISA kit (R & D, DY814-05); 3-Methyladenine (M9281), MG132 (M7449), DMSO (D2650), CQ (Chloroquine phosphate) (PHR1258), NH4Cl (09718), Earle’s balanced salt solution (EBSS), anti-Flag agarose affinity beads (A2220) and protein A/G agarose affinity beads (P6486/E3403) (Sigma); Glutathione Sepharose 4B (GE Healthcare, 17-0756-05); Bafilomycin A1(Baf-A1) (Selleck, S1314); DAPI (C1005), ZVAD (Caspase inhibitor Z-VAD-FMK, C1202), NP-40 (ST366), and recombinant Human IFN-β (P5660) (Beyotime). .. Human RNF5, ULK1, ATG13, FIP200, and ATG101 specific siRNAs were designed and synthesized by RiboBio Co. Ltd, China.

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