non targeting control dsrna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher non targeting control dsrna
    Non Targeting Control Dsrna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non targeting control dsrna/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    non targeting control dsrna - by Bioz Stars, 2020-07
    85/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The extraembryonic serosa protects the insect egg against desiccation
    Article Snippet: .. Non-targeting control dsRNA was synthesized from a 500 bp vector sequence (pCRII, Invitrogen) cloned with the forward primer 5′-TGCCGGATCAAGAGCTACCAA-3′ and the reverse primer 5′-TGTGAGCAAAAGGCCAGCAA-3′. dsRNA was synthesized using the MEGAscript RNAi kit (Ambion), and about 0.2 μl of a 0.5 μg μl−1 dsRNA solution was injected into pupae of the San Bernardino strain, according to Bucher et al. [ ]. .. Eggs were collected at 18–24 h old and put individually in a well of a 96-well plate, and incubated at 5, 20, 50, 65, 75 or 90 per cent RH and at 25°C, 30°C or 35°C (5° below, exactly at and 5° above the temperature at which our laboratory stock is kept, respectively).

    Transfection:

    Article Title: High Molecular Weight Tropomyosins Regulate Osteoclast Cytoskeletal Morphology
    Article Snippet: .. On day 5 of osteoclast differentiation, siRNA or a non-targeting control dsRNA at the same concentration (Ambion) was transfected with Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA). .. Transfection efficiency was measured by introducing a commercial fluorescent dsRNA (Sequitur/Invitrogen Corp.) into the cells; efficiency was > 95%, as previously described [ ].

    Synthesized:

    Article Title: The extraembryonic serosa protects the insect egg against desiccation
    Article Snippet: .. Non-targeting control dsRNA was synthesized from a 500 bp vector sequence (pCRII, Invitrogen) cloned with the forward primer 5′-TGCCGGATCAAGAGCTACCAA-3′ and the reverse primer 5′-TGTGAGCAAAAGGCCAGCAA-3′. dsRNA was synthesized using the MEGAscript RNAi kit (Ambion), and about 0.2 μl of a 0.5 μg μl−1 dsRNA solution was injected into pupae of the San Bernardino strain, according to Bucher et al. [ ]. .. Eggs were collected at 18–24 h old and put individually in a well of a 96-well plate, and incubated at 5, 20, 50, 65, 75 or 90 per cent RH and at 25°C, 30°C or 35°C (5° below, exactly at and 5° above the temperature at which our laboratory stock is kept, respectively).

    Concentration Assay:

    Article Title: High Molecular Weight Tropomyosins Regulate Osteoclast Cytoskeletal Morphology
    Article Snippet: .. On day 5 of osteoclast differentiation, siRNA or a non-targeting control dsRNA at the same concentration (Ambion) was transfected with Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA). .. Transfection efficiency was measured by introducing a commercial fluorescent dsRNA (Sequitur/Invitrogen Corp.) into the cells; efficiency was > 95%, as previously described [ ].

    Article Title: Tropomyosin 4 Regulates Adhesion Structures and Resorptive Capacity in Osteoclasts
    Article Snippet: .. On day 5 of GST-RANKL treatment, a solution of siRNA or a non-targeting control dsRNA at the same concentration (Ambion) was added to Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA) in plain DMEM without serum or antibiotics and added to the cells. ..

    Sequencing:

    Article Title: The extraembryonic serosa protects the insect egg against desiccation
    Article Snippet: .. Non-targeting control dsRNA was synthesized from a 500 bp vector sequence (pCRII, Invitrogen) cloned with the forward primer 5′-TGCCGGATCAAGAGCTACCAA-3′ and the reverse primer 5′-TGTGAGCAAAAGGCCAGCAA-3′. dsRNA was synthesized using the MEGAscript RNAi kit (Ambion), and about 0.2 μl of a 0.5 μg μl−1 dsRNA solution was injected into pupae of the San Bernardino strain, according to Bucher et al. [ ]. .. Eggs were collected at 18–24 h old and put individually in a well of a 96-well plate, and incubated at 5, 20, 50, 65, 75 or 90 per cent RH and at 25°C, 30°C or 35°C (5° below, exactly at and 5° above the temperature at which our laboratory stock is kept, respectively).

    Injection:

    Article Title: The extraembryonic serosa protects the insect egg against desiccation
    Article Snippet: .. Non-targeting control dsRNA was synthesized from a 500 bp vector sequence (pCRII, Invitrogen) cloned with the forward primer 5′-TGCCGGATCAAGAGCTACCAA-3′ and the reverse primer 5′-TGTGAGCAAAAGGCCAGCAA-3′. dsRNA was synthesized using the MEGAscript RNAi kit (Ambion), and about 0.2 μl of a 0.5 μg μl−1 dsRNA solution was injected into pupae of the San Bernardino strain, according to Bucher et al. [ ]. .. Eggs were collected at 18–24 h old and put individually in a well of a 96-well plate, and incubated at 5, 20, 50, 65, 75 or 90 per cent RH and at 25°C, 30°C or 35°C (5° below, exactly at and 5° above the temperature at which our laboratory stock is kept, respectively).

    Plasmid Preparation:

    Article Title: The extraembryonic serosa protects the insect egg against desiccation
    Article Snippet: .. Non-targeting control dsRNA was synthesized from a 500 bp vector sequence (pCRII, Invitrogen) cloned with the forward primer 5′-TGCCGGATCAAGAGCTACCAA-3′ and the reverse primer 5′-TGTGAGCAAAAGGCCAGCAA-3′. dsRNA was synthesized using the MEGAscript RNAi kit (Ambion), and about 0.2 μl of a 0.5 μg μl−1 dsRNA solution was injected into pupae of the San Bernardino strain, according to Bucher et al. [ ]. .. Eggs were collected at 18–24 h old and put individually in a well of a 96-well plate, and incubated at 5, 20, 50, 65, 75 or 90 per cent RH and at 25°C, 30°C or 35°C (5° below, exactly at and 5° above the temperature at which our laboratory stock is kept, respectively).

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    Thermo Fisher dsrna block it fluorescent oligo
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