non supercoiled dna a 2 7 kb  (Thermo Fisher)


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    Name:
    QuickConnect Mop Handles and Hooks
    Description:
    Choose from a lightweight anodized aluminum durable electropolished stainless steel or fiberglass Contec QuickConnect Mop Handles are available in fixed length or telescoping models to fit all mopping needs
    Catalog Number:
    19033781
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    Category:
    Lab Supplies Plastics Glassware
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    Structured Review

    Thermo Fisher non supercoiled dna a 2 7 kb
    Choose from a lightweight anodized aluminum durable electropolished stainless steel or fiberglass Contec QuickConnect Mop Handles are available in fixed length or telescoping models to fit all mopping needs
    https://www.bioz.com/result/non supercoiled dna a 2 7 kb/product/Thermo Fisher
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    non supercoiled dna a 2 7 kb - by Bioz Stars, 2020-09
    93/100 stars

    Related Products / Commonly Used Together

    nt.bstnbi nicking enzyme

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    Related Articles

    Isolation:

    Article Title: Sizing of single fluorescently stained DNA fragments by scanning microscopy
    Article Snippet: .. 14 200 bp plasmid DNA was isolated from EcoRI cells with the Plasmid Mini Kit (Qiagen, Germany). m13mp18 RF1 DNA (7250 bp), the plasmids pBR322 DNA (4361 bp) and pUC19 DNA (2686 bp) were purchased from MBI Fermentas, Germany. m13mp18 RF1 DNA was digested at 36°C for 4 h by the restriction enzymes EcoRI and PagI (MBI Fermentas, Germany) into 2318 and 4932 bp fragments. .. All fragment lengths were verified by gel electrophoresis (Fig. ).

    Purification:

    Article Title: Human RECQ1 Interacts with Ku70/80 and Modulates DNA End-Joining of Double-Strand Breaks
    Article Snippet: .. For preparation of EMSA template, 10 µg of the supercoiled pUC19 plasmid vector (2686 bp, Invitrogen) were digested with PvuII-HF endonuclease (NEB) at 37°C for 2 h, the resulting 322 bp and 2364 bp blunt-ended DNA fragments were resolved in 1% TAE agarose and the 322 bp DNA fragment subsequently purified using QIAquick Gel Extraction kit (Qiagen). .. Gel shift reactions utilized 30 ng of the 322 bp DNA fragment.

    Sample Prep:

    Article Title: Time-dependent bending rigidity and helical twist of DNA by rearrangement of bound HU protein
    Article Snippet: .. Sample preparation DNA fragments of 1000 bp and pUC19 (2686 bp) were purchased from Thermoscientific (Vilnius, Lithuania). ..

    Plasmid Preparation:

    Article Title: Sizing of single fluorescently stained DNA fragments by scanning microscopy
    Article Snippet: .. 14 200 bp plasmid DNA was isolated from EcoRI cells with the Plasmid Mini Kit (Qiagen, Germany). m13mp18 RF1 DNA (7250 bp), the plasmids pBR322 DNA (4361 bp) and pUC19 DNA (2686 bp) were purchased from MBI Fermentas, Germany. m13mp18 RF1 DNA was digested at 36°C for 4 h by the restriction enzymes EcoRI and PagI (MBI Fermentas, Germany) into 2318 and 4932 bp fragments. .. All fragment lengths were verified by gel electrophoresis (Fig. ).

    Article Title: Effects of physiological self-crowding of DNA on shape and biological properties of DNA molecules with various levels of supercoiling
    Article Snippet: .. Preparation of non-supercoiled DNA A 2.7 kb (pUC19 2686 bp) supercoiled plasmid DNA was purchased from Fermentas (Switzerland) and nicked using Nt.BstNBI nicking enzyme (NEB). .. Nicked plasmids were further extracted from 1.5% agarose gel and purified using extraction kit from Promega.

    Article Title: Human RECQ1 Interacts with Ku70/80 and Modulates DNA End-Joining of Double-Strand Breaks
    Article Snippet: .. For preparation of EMSA template, 10 µg of the supercoiled pUC19 plasmid vector (2686 bp, Invitrogen) were digested with PvuII-HF endonuclease (NEB) at 37°C for 2 h, the resulting 322 bp and 2364 bp blunt-ended DNA fragments were resolved in 1% TAE agarose and the 322 bp DNA fragment subsequently purified using QIAquick Gel Extraction kit (Qiagen). .. Gel shift reactions utilized 30 ng of the 322 bp DNA fragment.

    Gel Extraction:

    Article Title: Human RECQ1 Interacts with Ku70/80 and Modulates DNA End-Joining of Double-Strand Breaks
    Article Snippet: .. For preparation of EMSA template, 10 µg of the supercoiled pUC19 plasmid vector (2686 bp, Invitrogen) were digested with PvuII-HF endonuclease (NEB) at 37°C for 2 h, the resulting 322 bp and 2364 bp blunt-ended DNA fragments were resolved in 1% TAE agarose and the 322 bp DNA fragment subsequently purified using QIAquick Gel Extraction kit (Qiagen). .. Gel shift reactions utilized 30 ng of the 322 bp DNA fragment.