Review



nod2 conjugated with pe  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Novus Biologicals nod2 conjugated with pe
    FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and <t>NOD2-dependent</t> pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 <t>conjugated</t> with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.
    Nod2 Conjugated With Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nod2+conjugated+with+pe/10__3389_slash_fimmu__2024__1451315-54-45-55?v=Novus+Biologicals
    Average 93 stars, based on 4 article reviews
    nod2 conjugated with pe - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Muramyl dipeptide potentiates Staphylococcus aureus lipoteichoic acid-induced nitric oxide production via TLR2/NOD2/PAFR signaling pathways"

    Article Title: Muramyl dipeptide potentiates Staphylococcus aureus lipoteichoic acid-induced nitric oxide production via TLR2/NOD2/PAFR signaling pathways

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1451315

    FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and NOD2-dependent pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 conjugated with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.
    Figure Legend Snippet: FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and NOD2-dependent pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 conjugated with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.

    Techniques Used: Staining, Cytometry, Expressing, Control



    Similar Products

    nod2 conjugated with pe  (Novus Biologicals)
    93
    Novus Biologicals nod2 conjugated with pe
    FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and <t>NOD2-dependent</t> pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 <t>conjugated</t> with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.
    Nod2 Conjugated With Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nod2+conjugated+with+pe/10__3389_slash_fimmu__2024__1451315-54-45-55?v=Novus+Biologicals
    Average 93 stars, based on 1 article reviews
    nod2 conjugated with pe - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and NOD2-dependent pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 conjugated with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.

    Journal: Frontiers in Immunology

    Article Title: Muramyl dipeptide potentiates Staphylococcus aureus lipoteichoic acid-induced nitric oxide production via TLR2/NOD2/PAFR signaling pathways

    doi: 10.3389/fimmu.2024.1451315

    Figure Lengend Snippet: FIGURE 2 Enhanced SaLTA-induced NO production through MDP is mediated by TLR2 and CD14/MyD88-, and NOD2-dependent pathway. (A) RAW 264.7 cells (500 ml of 5 × 105 cells/ml) were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. The cells were then stained with anti- mouse TLR2 conjugated with PE or CD14 conjugated with FITC antibodies. For intracellular NOD2, the cells were permeabilized and stained with anti-mouse NOD2 conjugated with PE. The stained cells were then subjected to flow cytometry. Upper, a representative TLR2, NOD2, and CD14 expression determined by flow cytometric analysis. Values given in each histogram indicate mean fluorescence intensity (MFI) of 10,000 events of live cells in the treatment group. Lower, the expression of TLR2, NOD2, and CD14 is presented as mean of MFI ± S.D. of three replicates for each group. Asterisk (*) indicates a significant difference between the indicated treatment groups at P < 0.05. NT, non-treatment group; IC, isotype control. (B, C) BMMs (200 ml of 5 × 105 cells/ml) from wild-type, (B) TLR2-, MyD88- CD14- or (C) NOD2-deficient mice were treated with SaLTA (0 or 3 mg/ml) and/or MDP (0 or 10 mg/ml) for 24 h. Then, nitrite levels in the supernatants were measured. The data represent mean ± S.D. of three replicates for each group. * indicates a significant difference compared to the control group at P < 0.05.

    Article Snippet: For flow cytometric analysis, antibodies specific to TLR2 conjugated with phycoerythrin (PE, Catalog No. 12-9021-82), and to CD14 conjugated with fluorescein isothiocyanate (FITC, Catalog No. 123308) were obtained from eBioscience (San Diego, CA, USA) and Biolegend (San Diego, CA, USA), respectively, while antibody specific to NOD2 conjugated with PE (Catalog No. NB100-524PE) was purchased from Novus (Los Angeles, CA, USA).

    Techniques: Staining, Cytometry, Expressing, Control