shrimp alkaline phospatase  (New England Biolabs)


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  • 92
    Name:
    NEBNext Multiplex Oligos for Illumina Index Primers Set 4
    Description:
    NEBNext Multiplex Oligos for Illumina Index Primers Set 4 96 rxns
    Catalog Number:
    e7730l
    Price:
    385
    Size:
    96 rxns
    Category:
    Probes and Primers
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    Structured Review

    New England Biolabs shrimp alkaline phospatase
    NEBNext Multiplex Oligos for Illumina Index Primers Set 4
    NEBNext Multiplex Oligos for Illumina Index Primers Set 4 96 rxns
    https://www.bioz.com/result/shrimp alkaline phospatase/product/New England Biolabs
    Average 92 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    shrimp alkaline phospatase - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: High-Throughput Genotyping of CRISPR/Cas Edited Cells in 96-Well Plates
    Article Snippet: .. Reagents and Materials Fetal bovine serum, FBS (Thermo Fisher, Paisley, UK; Cat. no.: 10270-106) Dimethyl sulphoxide, DMSO (VWR, Lutterworth, UK; Cat. no.: 23500.260) 96-well V-bottomed plates (Sigma-Aldrich, Dorset, UK; Cat. no.: CLS3894) Parafilm (VWR, Lutterworth, UK; Cat. no.: PM-996) 96-well PCR plate (Thermo Fisher; Cat. no.: AB1400L) Tris, 1 M, pH 8.0 (Thermo Fisher; Cat. no.: AM9855G) Ethylenediaminetetraacetic acid (EDTA), 0.5 mM, pH 8.0 (Thermo Fisher; Cat. no.: 15575-038) Tween 20, 50% (Thermo Fisher; Cat. no.: 003005) PCR grade water (Thermo Fisher; Cat. no.: AM9932) DNA low bind tubes (Eppendorf, Arlington, UK; Cat. no.: Z666548) Proteinase K (Thermo Fisher; Cat. no.: EO0491) Platinum PCR master mix (Thermo Fisher; Cat. no.: 12532016) Locus specific primers, 10 µM mix, see (Sigma, St Louis, MO, USA or Integrated DNA Technologies (IDT), Skokie, Illinois, USA) Agarose (Roche, Burgess Hill, UK; Cat. no.: 11388983001) 10× Tris acetate-EDTA, TAE (Sigma-Aldrich; Cat. no.: 11666690001) 100 bp ladder (New England Biolabs, Hitchin, UK; Cat. no.: N0551G) Exonuclease I, Escherichia coli (New England Biolabs; Cat. no: M0293L) Shrimp alkaline phospatase, rSAP (New England Biolabs, Hitchin, UK; Cat. no.: M0371L) Custom iR5 and iC7 barcoded primers, (Sigma or IDT) Agencourt Ampure XP SPRI Beads (Beckman Coulter, High Wycombe, UK; Cat. no.: A63881) 100% Ethanol (VWR, Lutterworth, UK; Cat. no.: 20821.330) Qubit BR DNA assay kit (Thermo Fisher; Cat. no.: Q32850) NEB Ultra II (New England Biolabs; Cat. no.: 7645S/L) PCR Tube (Appleton Woods, Birmingham, UK; Cat. no.: TA571) NEB Next Multiplex Oligos for Illumina primer set 1 (New England Biolabs; Cat. no.: E7500S/L) NEB Next Multiplex Oligos for Illumina primer set 2 (New England Biolabs; Cat. no.: E7335S/L) Herculase II Fusion polymerase kit (Agilent, Cheadle, UK; Cat. no.: 600677) Tris-EDTA, TE (Sigma-Aldrich; Cat. no.: 99302) D1000 reagents (Agilent; Cat. no.: 50675583) D1000 loading tips (Agilent; Cat. no.: 50675153) D1000 screen tape (Agilent; Cat. no.: 50675582) KAPA Library Quantification Complete Kit (Roche; Cat. no.: KK4824) MiSeq Reagent Nano Kit, v2 500-Cycles (Illumina, Cambridge, UK; Cat. no.: MS-103-1003) PhiX Control v3 (Illumina; Cat. no.: FC-110-3001) .. Equipment 8- or 12-channel pipette (Labgene Scientific, Châtel-Saint-Denis, Switzerland: Cat. no.: 5121, 5125) Centrifuge with buckets for plates (Eppendorf; Cat. no.: 5810R) Thermocycler with 96-well plate capacity (Bio-Rad, Watford, UK; Cat. no.: T100) Electrophoresis gel tank and power pack Magnetic rack (DynaMag-2; Thermo Fisher; Cat. no.: 13221) Minifuge (Starlab, Milton Keynes, UK; Cat. no.: N2631-0007) Qubit fluorometer (Thermo Fisher; Cat. no.: Q33226) Agilent 2200 TapeStation (Agilent; Cat. no.: G2964AA) Real-time quantitative polymerase chain reaction (qPCR) thermocycler (Thermo Fisher StepOnePlus; Thermo Fisher; Cat. no.: 4376598) MiSeq (Illumina; Cat. no.: SY-410-1003) Microcentrifuge (Eppendorf; Cat. no.: 5424R)

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory sensory epithelium
    Article Snippet: .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB). .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB).

    Article Title: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation
    Article Snippet: .. The mRNA was converted into a cDNA library by using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), NEB Next Multiplex Oligos for Illumina Index Primers Set I (New England Biolabs), and an Agencourt AMPure XP PCR purification system (Beckman Coulter). .. Quality of the cDNA library was evaluated using an Agilent High Sensitivity DNA Kit and 2100 Bioanalyzer (Agilent Technologies).

    Amplification:

    Article Title: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
    Article Snippet: .. The wild-type template was amplified by PCR from E. coli DH5α genomic DNA using oligonucleotides 1, 2 and 3 followed by PCR amplification of the product with oligonucleotides 4 and 5 (see for the sequences of all oligonucleotides used). ..

    Methylation:

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory sensory epithelium
    Article Snippet: .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB). .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB).

    Next-Generation Sequencing:

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory sensory epithelium
    Article Snippet: .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB). .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB).

    Purification:

    Article Title: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation
    Article Snippet: .. The mRNA was converted into a cDNA library by using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), NEB Next Multiplex Oligos for Illumina Index Primers Set I (New England Biolabs), and an Agencourt AMPure XP PCR purification system (Beckman Coulter). .. Quality of the cDNA library was evaluated using an Agilent High Sensitivity DNA Kit and 2100 Bioanalyzer (Agilent Technologies).

    Produced:

    Article Title: DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory sensory epithelium
    Article Snippet: .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB). .. Illumina-compatible NGS libraries were produced using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (NEB) and Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1) (NEB).

    Polymerase Chain Reaction:

    Article Title: High-Throughput Genotyping of CRISPR/Cas Edited Cells in 96-Well Plates
    Article Snippet: .. Reagents and Materials Fetal bovine serum, FBS (Thermo Fisher, Paisley, UK; Cat. no.: 10270-106) Dimethyl sulphoxide, DMSO (VWR, Lutterworth, UK; Cat. no.: 23500.260) 96-well V-bottomed plates (Sigma-Aldrich, Dorset, UK; Cat. no.: CLS3894) Parafilm (VWR, Lutterworth, UK; Cat. no.: PM-996) 96-well PCR plate (Thermo Fisher; Cat. no.: AB1400L) Tris, 1 M, pH 8.0 (Thermo Fisher; Cat. no.: AM9855G) Ethylenediaminetetraacetic acid (EDTA), 0.5 mM, pH 8.0 (Thermo Fisher; Cat. no.: 15575-038) Tween 20, 50% (Thermo Fisher; Cat. no.: 003005) PCR grade water (Thermo Fisher; Cat. no.: AM9932) DNA low bind tubes (Eppendorf, Arlington, UK; Cat. no.: Z666548) Proteinase K (Thermo Fisher; Cat. no.: EO0491) Platinum PCR master mix (Thermo Fisher; Cat. no.: 12532016) Locus specific primers, 10 µM mix, see (Sigma, St Louis, MO, USA or Integrated DNA Technologies (IDT), Skokie, Illinois, USA) Agarose (Roche, Burgess Hill, UK; Cat. no.: 11388983001) 10× Tris acetate-EDTA, TAE (Sigma-Aldrich; Cat. no.: 11666690001) 100 bp ladder (New England Biolabs, Hitchin, UK; Cat. no.: N0551G) Exonuclease I, Escherichia coli (New England Biolabs; Cat. no: M0293L) Shrimp alkaline phospatase, rSAP (New England Biolabs, Hitchin, UK; Cat. no.: M0371L) Custom iR5 and iC7 barcoded primers, (Sigma or IDT) Agencourt Ampure XP SPRI Beads (Beckman Coulter, High Wycombe, UK; Cat. no.: A63881) 100% Ethanol (VWR, Lutterworth, UK; Cat. no.: 20821.330) Qubit BR DNA assay kit (Thermo Fisher; Cat. no.: Q32850) NEB Ultra II (New England Biolabs; Cat. no.: 7645S/L) PCR Tube (Appleton Woods, Birmingham, UK; Cat. no.: TA571) NEB Next Multiplex Oligos for Illumina primer set 1 (New England Biolabs; Cat. no.: E7500S/L) NEB Next Multiplex Oligos for Illumina primer set 2 (New England Biolabs; Cat. no.: E7335S/L) Herculase II Fusion polymerase kit (Agilent, Cheadle, UK; Cat. no.: 600677) Tris-EDTA, TE (Sigma-Aldrich; Cat. no.: 99302) D1000 reagents (Agilent; Cat. no.: 50675583) D1000 loading tips (Agilent; Cat. no.: 50675153) D1000 screen tape (Agilent; Cat. no.: 50675582) KAPA Library Quantification Complete Kit (Roche; Cat. no.: KK4824) MiSeq Reagent Nano Kit, v2 500-Cycles (Illumina, Cambridge, UK; Cat. no.: MS-103-1003) PhiX Control v3 (Illumina; Cat. no.: FC-110-3001) .. Equipment 8- or 12-channel pipette (Labgene Scientific, Châtel-Saint-Denis, Switzerland: Cat. no.: 5121, 5125) Centrifuge with buckets for plates (Eppendorf; Cat. no.: 5810R) Thermocycler with 96-well plate capacity (Bio-Rad, Watford, UK; Cat. no.: T100) Electrophoresis gel tank and power pack Magnetic rack (DynaMag-2; Thermo Fisher; Cat. no.: 13221) Minifuge (Starlab, Milton Keynes, UK; Cat. no.: N2631-0007) Qubit fluorometer (Thermo Fisher; Cat. no.: Q33226) Agilent 2200 TapeStation (Agilent; Cat. no.: G2964AA) Real-time quantitative polymerase chain reaction (qPCR) thermocycler (Thermo Fisher StepOnePlus; Thermo Fisher; Cat. no.: 4376598) MiSeq (Illumina; Cat. no.: SY-410-1003) Microcentrifuge (Eppendorf; Cat. no.: 5424R)

    Article Title: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
    Article Snippet: .. The wild-type template was amplified by PCR from E. coli DH5α genomic DNA using oligonucleotides 1, 2 and 3 followed by PCR amplification of the product with oligonucleotides 4 and 5 (see for the sequences of all oligonucleotides used). ..

    Article Title: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation
    Article Snippet: .. The mRNA was converted into a cDNA library by using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), NEB Next Multiplex Oligos for Illumina Index Primers Set I (New England Biolabs), and an Agencourt AMPure XP PCR purification system (Beckman Coulter). .. Quality of the cDNA library was evaluated using an Agilent High Sensitivity DNA Kit and 2100 Bioanalyzer (Agilent Technologies).

    cDNA Library Assay:

    Article Title: Bradyrhizobium diazoefficiens USDA110 PhaR functions for pleiotropic regulation of cellular processes besides PHB accumulation
    Article Snippet: .. The mRNA was converted into a cDNA library by using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), NEB Next Multiplex Oligos for Illumina Index Primers Set I (New England Biolabs), and an Agencourt AMPure XP PCR purification system (Beckman Coulter). .. Quality of the cDNA library was evaluated using an Agilent High Sensitivity DNA Kit and 2100 Bioanalyzer (Agilent Technologies).

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  • 99
    New England Biolabs no e7500s l neb next multiplex oligos for illumina primer set 2
    No E7500s L Neb Next Multiplex Oligos For Illumina Primer Set 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/no e7500s l neb next multiplex oligos for illumina primer set 2/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    no e7500s l neb next multiplex oligos for illumina primer set 2 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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