Structured Review

FUJIFILM nifedipine
Voltage-dependent Ba 2+ currents in the epithelioid cells Epithelioid cells were voltage clamped at -70 mV in the presence of tetrodotoxin (0.2 μM) and BaCl 2 (5 mM) instead of CaCl 2 . A , representative recordings of current responses of an epithelioid cell to depolarizing potentials for 30 ms between -60 and +60 mV in 10 mV increments before (Control) and during exposure to CoCl 2 (2 mM), and after its removal (Washout). B , peak inward current-voltage relationships were obtained from the cell before (▪) and during (•) exposure to CoCl 2 , and after its removal (▴). C , effects of <t>nifedipine</t> and ω-conotoxin GVIA on peak Ba 2+ currents in an epithelioid cell. At -70 mV in the presence of tetrodotoxin (0.2 μM), TEA-Cl (5 mM) and BaCl 2 (5 mM), Ba 2+ currents were evoked by depolarizing steps for 15 ms to +10 mV with 20 s intervals. The peak Ba 2+ currents are expressed as percentages of the first Ba 2+ current recorded in the absence of blockers and plotted against time. Nifedipine (1 μM) and ω-conotoxin GVIA (1 μM) were applied during the periods indicated by horizontal lines starting with an arrow. Inset, superimposed current traces with letters corresponding to those in the peak current trace. The dotted line indicates the zero current level.
Nifedipine, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Characteristics of 5-HT-containing chemoreceptor cells of the chicken aortic body"

Article Title: Characteristics of 5-HT-containing chemoreceptor cells of the chicken aortic body

Journal: The Journal of Physiology

doi: 10.1111/j.1469-7793.1999.049ad.x

Voltage-dependent Ba 2+ currents in the epithelioid cells Epithelioid cells were voltage clamped at -70 mV in the presence of tetrodotoxin (0.2 μM) and BaCl 2 (5 mM) instead of CaCl 2 . A , representative recordings of current responses of an epithelioid cell to depolarizing potentials for 30 ms between -60 and +60 mV in 10 mV increments before (Control) and during exposure to CoCl 2 (2 mM), and after its removal (Washout). B , peak inward current-voltage relationships were obtained from the cell before (▪) and during (•) exposure to CoCl 2 , and after its removal (▴). C , effects of nifedipine and ω-conotoxin GVIA on peak Ba 2+ currents in an epithelioid cell. At -70 mV in the presence of tetrodotoxin (0.2 μM), TEA-Cl (5 mM) and BaCl 2 (5 mM), Ba 2+ currents were evoked by depolarizing steps for 15 ms to +10 mV with 20 s intervals. The peak Ba 2+ currents are expressed as percentages of the first Ba 2+ current recorded in the absence of blockers and plotted against time. Nifedipine (1 μM) and ω-conotoxin GVIA (1 μM) were applied during the periods indicated by horizontal lines starting with an arrow. Inset, superimposed current traces with letters corresponding to those in the peak current trace. The dotted line indicates the zero current level.
Figure Legend Snippet: Voltage-dependent Ba 2+ currents in the epithelioid cells Epithelioid cells were voltage clamped at -70 mV in the presence of tetrodotoxin (0.2 μM) and BaCl 2 (5 mM) instead of CaCl 2 . A , representative recordings of current responses of an epithelioid cell to depolarizing potentials for 30 ms between -60 and +60 mV in 10 mV increments before (Control) and during exposure to CoCl 2 (2 mM), and after its removal (Washout). B , peak inward current-voltage relationships were obtained from the cell before (▪) and during (•) exposure to CoCl 2 , and after its removal (▴). C , effects of nifedipine and ω-conotoxin GVIA on peak Ba 2+ currents in an epithelioid cell. At -70 mV in the presence of tetrodotoxin (0.2 μM), TEA-Cl (5 mM) and BaCl 2 (5 mM), Ba 2+ currents were evoked by depolarizing steps for 15 ms to +10 mV with 20 s intervals. The peak Ba 2+ currents are expressed as percentages of the first Ba 2+ current recorded in the absence of blockers and plotted against time. Nifedipine (1 μM) and ω-conotoxin GVIA (1 μM) were applied during the periods indicated by horizontal lines starting with an arrow. Inset, superimposed current traces with letters corresponding to those in the peak current trace. The dotted line indicates the zero current level.

Techniques Used: Mass Spectrometry

2) Product Images from "The dihydropyridine calcium channel blocker benidipine prevents lysophosphatidylcholine-induced endothelial dysfunction in rat aorta"

Article Title: The dihydropyridine calcium channel blocker benidipine prevents lysophosphatidylcholine-induced endothelial dysfunction in rat aorta

Journal: Journal of Biomedical Science

doi: 10.1186/1423-0127-16-57

Effects of benidipine (A), amlodipine (B) and nifedipine (C) on LPC-induced attenuation of endothelium-dependent relaxation . Drugs were administered orally to rats. Rats were treated as described for Figure 1. Data are expressed as percentage of PE-induced contraction. Each value represents the mean ± S.E. of 6–10 experiments. *P
Figure Legend Snippet: Effects of benidipine (A), amlodipine (B) and nifedipine (C) on LPC-induced attenuation of endothelium-dependent relaxation . Drugs were administered orally to rats. Rats were treated as described for Figure 1. Data are expressed as percentage of PE-induced contraction. Each value represents the mean ± S.E. of 6–10 experiments. *P

Techniques Used:

3) Product Images from "Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice"

Article Title: Protective and therapeutic effect of felodipine against bleomycin-induced pulmonary fibrosis in mice

Journal: Scientific Reports

doi: 10.1038/s41598-017-03676-y

Effect of other calcium channel blockers on TGF-β1-induced collagen production. LL29 cells were incubated with TGF-β1 (5 ng/ml) for 48 h ( a ) or 24 h ( b ) in the presence of indicated concentrations (µM) of nifedipine (Nif) or benidipine (Beni). Level of collagen in culture medium was determined by Sircol assay ( a ). Viable cell number was determined by MTT method ( b ). Values represent mean ± S.E.M. * * or ## P
Figure Legend Snippet: Effect of other calcium channel blockers on TGF-β1-induced collagen production. LL29 cells were incubated with TGF-β1 (5 ng/ml) for 48 h ( a ) or 24 h ( b ) in the presence of indicated concentrations (µM) of nifedipine (Nif) or benidipine (Beni). Level of collagen in culture medium was determined by Sircol assay ( a ). Viable cell number was determined by MTT method ( b ). Values represent mean ± S.E.M. * * or ## P

Techniques Used: Incubation, MTT Assay

Effect of other calcium blockers on bleomycin-induced pulmonary fibrosis, alteration of lung mechanics, and respiratory dysfunction. Mice were intratracheally administered with bleomycin (BLM, 2 mg/kg) or vehicle once only on day 0. Mice were intratracheally administered indicated doses (mg/kg) of nifedipine (Nif) or benidipine (Beni) once daily for 14 days (from day 0–13). Sections of pulmonary tissue were prepared on day 14 (24 h after final calcium blockers administration) and subjected to histopathological examination. H E staining (upper images) and Masson’s trichrome staining (lower images); scale bar = 1.0 mm ( a ). Percentage of collagen-positive area was determined based on images of Masson’s trichrome staining ( b ). Pulmonary hydroxyproline level was determined on day 14 ( c ). Total respiratory system elastance and FVC were measured on day 14 ( d ). Values represent mean ± S.E.M. * * or ## P
Figure Legend Snippet: Effect of other calcium blockers on bleomycin-induced pulmonary fibrosis, alteration of lung mechanics, and respiratory dysfunction. Mice were intratracheally administered with bleomycin (BLM, 2 mg/kg) or vehicle once only on day 0. Mice were intratracheally administered indicated doses (mg/kg) of nifedipine (Nif) or benidipine (Beni) once daily for 14 days (from day 0–13). Sections of pulmonary tissue were prepared on day 14 (24 h after final calcium blockers administration) and subjected to histopathological examination. H E staining (upper images) and Masson’s trichrome staining (lower images); scale bar = 1.0 mm ( a ). Percentage of collagen-positive area was determined based on images of Masson’s trichrome staining ( b ). Pulmonary hydroxyproline level was determined on day 14 ( c ). Total respiratory system elastance and FVC were measured on day 14 ( d ). Values represent mean ± S.E.M. * * or ## P

Techniques Used: Mouse Assay, Staining

Related Articles

Concentration Assay:

Article Title: Isolation of CYP3A4 Inhibitors from the Black Cohosh (Cimicifuga racemosa)
Article Snippet: .. Various amounts (0–10 µM, final concentration) of samples in 1 ml of dimethylsulfoxide (DMSO) were added to 192 µl of solution containing 100 mM phosphate buffer (pH 7.4) containing 50 µM nifedipine (Wako Pure Chemical Industries, Ltd, Osaka, Japan), 5 mM glucose-6-phosphate (Oriental Yeast Co., Ltd, Tokyo, Japan), 0.5 mM b-NADP+ (Oriental Yeast Co., Ltd), 0.5 mM MgCl2 and 4.3 µg/ml glucose-6-phosphate dehydrogenase (Oriental Yeast Co., Ltd) and incubated at 37°C for 5 min. CYP3A4 (Gentest Co., Woburn, MA) was also pre-incubated in 7 µl of the buffer at 37°C for 5 min and added to the sample solution. .. After the incubation at 37°C for 1 h, the reaction was quenched by the addition of 100 ml of MeOH.

Incubation:

Article Title: Isolation of CYP3A4 Inhibitors from the Black Cohosh (Cimicifuga racemosa)
Article Snippet: .. Various amounts (0–10 µM, final concentration) of samples in 1 ml of dimethylsulfoxide (DMSO) were added to 192 µl of solution containing 100 mM phosphate buffer (pH 7.4) containing 50 µM nifedipine (Wako Pure Chemical Industries, Ltd, Osaka, Japan), 5 mM glucose-6-phosphate (Oriental Yeast Co., Ltd, Tokyo, Japan), 0.5 mM b-NADP+ (Oriental Yeast Co., Ltd), 0.5 mM MgCl2 and 4.3 µg/ml glucose-6-phosphate dehydrogenase (Oriental Yeast Co., Ltd) and incubated at 37°C for 5 min. CYP3A4 (Gentest Co., Woburn, MA) was also pre-incubated in 7 µl of the buffer at 37°C for 5 min and added to the sample solution. .. After the incubation at 37°C for 1 h, the reaction was quenched by the addition of 100 ml of MeOH.

Blocking Assay:

Article Title: Enhanced Fast Synaptic Transmission and a Delayed Depolarization Induced by Transient Potassium Current Blockade in Rat Hippocampal Slice as Studied by Optical Recording
Article Snippet: .. Nifedipine and Tg were obtained from Wako Pure Chemicals; channel blocking peptides were from Peptide Institute; all other reagents were from local suppliers. .. The MOS-based solid-state camera (128 × 128 elements) with frame memory and associated software has been described in detail in previous publications ( ; ).

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