ngs truseq custom amplicon low input  (Illumina Inc)

 
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    Name:
    TruSeq Custom Amplicon Filter Plate
    Description:
    The TruSight Myeloid Sequencing Panel uses expert defined content and proven next generation sequencing NGS technology to identify somatic variants in myeloid malignancies The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in Acute myeloid leukemia AML Myelodysplastic syndrome MDS Myeloproliferative neoplasms MPN Chronic myelogenous leukemia CML Chronic myelomonocytic leukemia CMML Juvenile myelomonocytic leukemia JMML The TruSight Myeloid Sequencing Panel covers 15 full genes exons only and 39 additional genes where oncogenic hotspots are covered providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test The result is an accurate cost effective solution that enables researchers to profile liquid tumors View the gene list TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller Filtering and annotation can then be performed using BaseSpace Variant Interpreter See All Illumina Cancer Research Panels
    Catalog Number:
    fc-130-1006
    Price:
    None
    Category:
    Clinical Research Products
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    Structured Review

    Illumina Inc ngs truseq custom amplicon low input
    TruSeq Custom Amplicon Filter Plate
    The TruSight Myeloid Sequencing Panel uses expert defined content and proven next generation sequencing NGS technology to identify somatic variants in myeloid malignancies The panel content was designed by a consortium of recognized experts in blood cancer disorders and targets genes frequently mutated in Acute myeloid leukemia AML Myelodysplastic syndrome MDS Myeloproliferative neoplasms MPN Chronic myelogenous leukemia CML Chronic myelomonocytic leukemia CMML Juvenile myelomonocytic leukemia JMML The TruSight Myeloid Sequencing Panel covers 15 full genes exons only and 39 additional genes where oncogenic hotspots are covered providing a comprehensive assessment of the key genes known to be involved in myeloid malignancies in a single test The result is an accurate cost effective solution that enables researchers to profile liquid tumors View the gene list TruSight Myeloid libraries are ideally suited to run on a desktop sequencer and perform alignment and variant calling with the MiSeq Reporter or Local Run Manager Amplicon workflow with Somatic Variant Caller Filtering and annotation can then be performed using BaseSpace Variant Interpreter See All Illumina Cancer Research Panels
    https://www.bioz.com/result/ngs truseq custom amplicon low input/product/Illumina Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngs truseq custom amplicon low input - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Molecular profiling of advanced solid tumors and patient outcomes with genotype-matched clinical trials: the Princess Margaret IMPACT/COMPACT trial
    Article Snippet: .. Three molecular profiling assays were used over the study period: a custom multiplex genotyping panel on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass-spectrometry platform (MassARRAY, Agena Bioscience, San Diego, CA, USA) to genotype 279 mutations within 23 genes (Additional file : Table S1); the TruSeq Amplicon Cancer Panel (TSACP, Illumina) on the MiSeq sequencer (Illumina) covering regions of 48 genes (Additional file : Table S2); and the Ion AmpliSeq Cancer Panel (ASCP, ThermoFisher Scientific) on the Ion Proton sequencer (ThermoFisher Scientific) covering regions of 50 genes (Additional file : Table S3). .. For more in-depth methodology on molecular profiling assays, including sequence alignment and base calling, see Additional file : Supplementary Methods.

    Amplification:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Exome sequencing studies at moderate (approximately 100X) depth rely on read position to identify potential PCR duplicates [ ], but amplicon-based (molecular inversion probes [ ], RainDrop Digital PCR (RainDance Technologies, Billerica, MA, USA), TruSeq Custom Amplicon (Illumina, San Diego, CA, USA)) methods commonly used for targeted sequencing have reads with the same start and stop positions. .. Hybridization-based methods, when sequenced deeply, can result in reads that are not PCR duplicates but have the same start stop locations by chance [ ].

    Article Title: Genomic profiling of stage II and III colon cancers reveals APC mutations to be associated with survival in stage III colon cancer patients
    Article Snippet: .. Detection of gene mutations Mutation status of APC , TP53 , KRAS , PIK3CA , FBXW7 , SMAD4 , BRAF and NRAS , i.e. genes that are commonly mutated in CRC, was assessed by next generation sequencing analysis of FFPE DNA samples using the TruSeq Amplicon Cancer Panel (TSACP; Illumina Inc, San Diego, CA USA). ..

    Article Title: Molecular profiling of advanced solid tumors and patient outcomes with genotype-matched clinical trials: the Princess Margaret IMPACT/COMPACT trial
    Article Snippet: .. Three molecular profiling assays were used over the study period: a custom multiplex genotyping panel on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass-spectrometry platform (MassARRAY, Agena Bioscience, San Diego, CA, USA) to genotype 279 mutations within 23 genes (Additional file : Table S1); the TruSeq Amplicon Cancer Panel (TSACP, Illumina) on the MiSeq sequencer (Illumina) covering regions of 48 genes (Additional file : Table S2); and the Ion AmpliSeq Cancer Panel (ASCP, ThermoFisher Scientific) on the Ion Proton sequencer (ThermoFisher Scientific) covering regions of 50 genes (Additional file : Table S3). .. For more in-depth methodology on molecular profiling assays, including sequence alignment and base calling, see Additional file : Supplementary Methods.

    Article Title: Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2
    Article Snippet: .. To determine the accuracy of variant detection, we assessed the degree of concordance of the variants detected by SLIMamp in NGS with the results obtained with Sanger sequencing and TruSeq Custom Amplicon (TSCA) (Illumina, San Francisco, CA) in 33 of the samples and Genome in a Bottle (GIAB, NA12878). ..

    Article Title: P-glycoprotein Mediates Ceritinib Resistance in Anaplastic Lymphoma Kinase-rearranged Non-small Cell Lung Cancer
    Article Snippet: .. 2.5 Deep Sequencing The samples were processed on the MiSeq platform with a TruSeq Amplicon Cancer Panel (Illumina, Inc.), which is designed to detect well-known somatic mutations of 48 genes (Table S2). .. Somatic variants were called using the Somatic Variant Caller (Illumina, Inc.) and annotated using the ANNOVAR ( ) software tool ( http://www.openbioinformatics.org/annovar/ ) and in-house scripts.

    Article Title: Recurrent and functional regulatory mutations in breast cancer
    Article Snippet: .. We designed a targeted amplicon assay (Illumina TruSeq Custom Amplicon) to validate several recurrently mutated promoter mutations in 47 patients from our initial cohort and 46 additional breast cancer cell lines (median coverage across samples and genes was approximately 3,900×). ..

    Article Title: Molecular profiling of advanced solid tumors and patient outcomes with genotype-matched clinical trials: the Princess Margaret IMPACT/COMPACT trial
    Article Snippet: .. TruSeq Amplicon Cancer Panel ..

    Article Title: Development and validation of a targeted gene sequencing panel for application to disparate cancers
    Article Snippet: .. Firstly, genes were selected from the following commercially available panels: TruSight Tumour 26 and TruSeq Amplicon Cancer Panels (Illumina), SureSeq Solid Tumour panel (Oxford Gene Technology), Foundation One Panel (Foundation Medicine), OncoCarta Panels Versions 1–3 (OncoCarta) and Haloplex Cancer Research Panel (Agilent). ..

    Mutagenesis:

    Article Title: Genomic profiling of stage II and III colon cancers reveals APC mutations to be associated with survival in stage III colon cancer patients
    Article Snippet: .. Detection of gene mutations Mutation status of APC , TP53 , KRAS , PIK3CA , FBXW7 , SMAD4 , BRAF and NRAS , i.e. genes that are commonly mutated in CRC, was assessed by next generation sequencing analysis of FFPE DNA samples using the TruSeq Amplicon Cancer Panel (TSACP; Illumina Inc, San Diego, CA USA). ..

    Next-Generation Sequencing:

    Article Title: Genomic profiling of stage II and III colon cancers reveals APC mutations to be associated with survival in stage III colon cancer patients
    Article Snippet: .. Detection of gene mutations Mutation status of APC , TP53 , KRAS , PIK3CA , FBXW7 , SMAD4 , BRAF and NRAS , i.e. genes that are commonly mutated in CRC, was assessed by next generation sequencing analysis of FFPE DNA samples using the TruSeq Amplicon Cancer Panel (TSACP; Illumina Inc, San Diego, CA USA). ..

    Article Title: Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2
    Article Snippet: .. To determine the accuracy of variant detection, we assessed the degree of concordance of the variants detected by SLIMamp in NGS with the results obtained with Sanger sequencing and TruSeq Custom Amplicon (TSCA) (Illumina, San Francisco, CA) in 33 of the samples and Genome in a Bottle (GIAB, NA12878). ..

    Digital PCR:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Exome sequencing studies at moderate (approximately 100X) depth rely on read position to identify potential PCR duplicates [ ], but amplicon-based (molecular inversion probes [ ], RainDrop Digital PCR (RainDance Technologies, Billerica, MA, USA), TruSeq Custom Amplicon (Illumina, San Diego, CA, USA)) methods commonly used for targeted sequencing have reads with the same start and stop positions. .. Hybridization-based methods, when sequenced deeply, can result in reads that are not PCR duplicates but have the same start stop locations by chance [ ].

    Sequencing:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Exome sequencing studies at moderate (approximately 100X) depth rely on read position to identify potential PCR duplicates [ ], but amplicon-based (molecular inversion probes [ ], RainDrop Digital PCR (RainDance Technologies, Billerica, MA, USA), TruSeq Custom Amplicon (Illumina, San Diego, CA, USA)) methods commonly used for targeted sequencing have reads with the same start and stop positions. .. Hybridization-based methods, when sequenced deeply, can result in reads that are not PCR duplicates but have the same start stop locations by chance [ ].

    Article Title: Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2
    Article Snippet: .. To determine the accuracy of variant detection, we assessed the degree of concordance of the variants detected by SLIMamp in NGS with the results obtained with Sanger sequencing and TruSeq Custom Amplicon (TSCA) (Illumina, San Francisco, CA) in 33 of the samples and Genome in a Bottle (GIAB, NA12878). ..

    Article Title: P-glycoprotein Mediates Ceritinib Resistance in Anaplastic Lymphoma Kinase-rearranged Non-small Cell Lung Cancer
    Article Snippet: .. 2.5 Deep Sequencing The samples were processed on the MiSeq platform with a TruSeq Amplicon Cancer Panel (Illumina, Inc.), which is designed to detect well-known somatic mutations of 48 genes (Table S2). .. Somatic variants were called using the Somatic Variant Caller (Illumina, Inc.) and annotated using the ANNOVAR ( ) software tool ( http://www.openbioinformatics.org/annovar/ ) and in-house scripts.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Genomic profiling of stage II and III colon cancers reveals APC mutations to be associated with survival in stage III colon cancer patients
    Article Snippet: .. Detection of gene mutations Mutation status of APC , TP53 , KRAS , PIK3CA , FBXW7 , SMAD4 , BRAF and NRAS , i.e. genes that are commonly mutated in CRC, was assessed by next generation sequencing analysis of FFPE DNA samples using the TruSeq Amplicon Cancer Panel (TSACP; Illumina Inc, San Diego, CA USA). ..

    Mass Spectrometry:

    Article Title: Molecular profiling of advanced solid tumors and patient outcomes with genotype-matched clinical trials: the Princess Margaret IMPACT/COMPACT trial
    Article Snippet: .. Three molecular profiling assays were used over the study period: a custom multiplex genotyping panel on a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass-spectrometry platform (MassARRAY, Agena Bioscience, San Diego, CA, USA) to genotype 279 mutations within 23 genes (Additional file : Table S1); the TruSeq Amplicon Cancer Panel (TSACP, Illumina) on the MiSeq sequencer (Illumina) covering regions of 48 genes (Additional file : Table S2); and the Ion AmpliSeq Cancer Panel (ASCP, ThermoFisher Scientific) on the Ion Proton sequencer (ThermoFisher Scientific) covering regions of 50 genes (Additional file : Table S3). .. For more in-depth methodology on molecular profiling assays, including sequence alignment and base calling, see Additional file : Supplementary Methods.

    Polymerase Chain Reaction:

    Article Title: Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments
    Article Snippet: .. Exome sequencing studies at moderate (approximately 100X) depth rely on read position to identify potential PCR duplicates [ ], but amplicon-based (molecular inversion probes [ ], RainDrop Digital PCR (RainDance Technologies, Billerica, MA, USA), TruSeq Custom Amplicon (Illumina, San Diego, CA, USA)) methods commonly used for targeted sequencing have reads with the same start and stop positions. .. Hybridization-based methods, when sequenced deeply, can result in reads that are not PCR duplicates but have the same start stop locations by chance [ ].

    Variant Assay:

    Article Title: Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2
    Article Snippet: .. To determine the accuracy of variant detection, we assessed the degree of concordance of the variants detected by SLIMamp in NGS with the results obtained with Sanger sequencing and TruSeq Custom Amplicon (TSCA) (Illumina, San Francisco, CA) in 33 of the samples and Genome in a Bottle (GIAB, NA12878). ..

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  • 99
    Illumina Inc truseq custom amplicon low input kit
    Truseq Custom Amplicon Low Input Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq custom amplicon low input kit/product/Illumina Inc
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    99
    Illumina Inc truseq custom amplicon low input library preparation protocol
    Truseq Custom Amplicon Low Input Library Preparation Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq custom amplicon low input library preparation protocol/product/Illumina Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truseq custom amplicon low input library preparation protocol - by Bioz Stars, 2021-01
    99/100 stars
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    97
    Illumina Inc the truseq custom amplicon low input library prep kit v2
    The Truseq Custom Amplicon Low Input Library Prep Kit V2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the truseq custom amplicon low input library prep kit v2/product/Illumina Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    the truseq custom amplicon low input library prep kit v2 - by Bioz Stars, 2021-01
    97/100 stars
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