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Illumina Inc nextseq 500 sequencer 75 bp single end
Nextseq 500 Sequencer 75 Bp Single End, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nextseq 500 sequencer 75 bp single end/product/Illumina Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nextseq 500 sequencer 75 bp single end - by Bioz Stars, 2020-02
86/100 stars

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Clone Assay:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End. .. RNA sequencing in G144 cells was done in technical triplicates for parental cells and technical duplicates for one WT and two CIC KO clones (KO8, KO9).

RNA Sequencing Assay:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Paragraph title: RNA-sequencing ... Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

Purification:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: DNA was eluted with elution-buffer (1% SDS, 0.1 M sodium bicarbonate) at 65°C overnight and purified using QIAquick PCR Purification Kit (Quiagen). .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

SYBR Green Assay:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: DNA was analyzed using the LightCycler® 480 SYBR Green I system (Roche). qPCR was performed in technical triplicates. .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

Sequencing:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End. .. Sequencing data was aligned via Tophat2 ( ) and differential expression was called using DESeq2 ( ).

Incubation:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Next day, beads and antibody/chromatin-mixture were incubated for 3 hours at 4°C. .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

Expressing:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End. .. Sequencing data was aligned via Tophat2 ( ) and differential expression was called using DESeq2 ( ).

Polymerase Chain Reaction:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: DNA was eluted with elution-buffer (1% SDS, 0.1 M sodium bicarbonate) at 65°C overnight and purified using QIAquick PCR Purification Kit (Quiagen). .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

Real-time Polymerase Chain Reaction:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: DNA was analyzed using the LightCycler® 480 SYBR Green I system (Roche). qPCR was performed in technical triplicates. .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

Chromatin Immunoprecipitation:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

ChIP-sequencing:

Article Title: The tumor suppressor CIC directly regulates MAPK pathway genes via histone deacetylation
Article Snippet: For ChIP-seq 1-2 ng of ChIP DNA was used for library preparation, using the NEBNext Ultra II DNA library prep kit (E7370; NEB). .. Libraries were sequenced using Illumina NextSeq 500 sequencer 75 bp Single End.

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