Structured Review

Illumina Inc nextera xt library preparation protocol
Nextera Xt Library Preparation Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nextera xt library preparation protocol/product/Illumina Inc
Average 99 stars, based on 21 article reviews
Price from $9.99 to $1999.99
nextera xt library preparation protocol - by Bioz Stars, 2020-09
99/100 stars

Images

Related Articles

DNA Extraction:

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
Article Snippet: .. Next generation sequencing VPRI 18536 Two library preparation kits, Illumina Nextera XT® (San Diego, California, USA) and NuGen Ovation® ultralow System V2 (San Carlos, California, USA), were compared using DNA extracts from the 13 DNA extraction protocols applied to VPRI specimen 18536. .. The genomic libraries were quantified by Promega Quantus™ fluorometer and Agilent 2200 TapeStation® and submitted for Illumina® HiSeq 3000 sequencing, except for DneP+ Illumina Nextera XT® and NuGen Ovation® ultralow System V2 libraries which were sequenced using Illumina® MiSeq V3 due to the Illumina® HiSeq 3000 being unavailable.

Amplification:

Article Title: 13-gene DNA Methylation Analysis from Oral Brushing: A Promising Non Invasive Tool in the Follow-up of Oral Cancer Patients
Article Snippet: .. Libraries were prepared using the Nextera™ Index Kit (Illumina, San Diego, CA, USA) by a locus-specific bisulfite amplicon approach [ ] and loaded onto MiSEQ (Illumina, San Diego, CA, USA, cod. ..

Synthesized:

Article Title: Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression
Article Snippet: .. OS25 cell suspension was loaded on 10–17 micron C1 Single-Cell Auto Prep IFC, Fluidigm; cDNA was synthesized in the chip using Clontech SMARTer Kit, and Illumina sequencing libraries were prepared with Nextera XT Kit and Nextera Index Kit (Illumina). .. Libraries from 96 cells were pooled and sequenced on four lanes on Illumina HiSeq2000 using 100 bp paired-end protocol.

Next-Generation Sequencing:

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
Article Snippet: .. Next generation sequencing VPRI 18536 Two library preparation kits, Illumina Nextera XT® (San Diego, California, USA) and NuGen Ovation® ultralow System V2 (San Carlos, California, USA), were compared using DNA extracts from the 13 DNA extraction protocols applied to VPRI specimen 18536. .. The genomic libraries were quantified by Promega Quantus™ fluorometer and Agilent 2200 TapeStation® and submitted for Illumina® HiSeq 3000 sequencing, except for DneP+ Illumina Nextera XT® and NuGen Ovation® ultralow System V2 libraries which were sequenced using Illumina® MiSeq V3 due to the Illumina® HiSeq 3000 being unavailable.

Sequencing:

Article Title: Targeted single molecule mutation detection with massively parallel sequencing
Article Snippet: .. Sequencing specific S288C laboratory strain In order to establish a highly accurate reference sequence for variant calling, the specific strain of S288C used in this study was sequenced using the Nextera XT kit (Illumina). .. Input DNA was taken from the untreated yeast grown on the same day as the mutagenesis experiment in order to ensure all spontaneous mutations occurring during culture were sampled.

Article Title: A Survey of Virus Recombination Uncovers Canonical Features of Artificial Chimeras Generated During Deep Sequencing Library Preparation
Article Snippet: .. Our attempt to detect biological recombination between the genomes of dengue virus (DENV; +ssRNA genome) and its mosquito host using the Illumina Nextera sequencing library preparation kit revealed that most, if not all, detected host–virus chimeras were artificial. ..

Article Title: Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression
Article Snippet: .. OS25 cell suspension was loaded on 10–17 micron C1 Single-Cell Auto Prep IFC, Fluidigm; cDNA was synthesized in the chip using Clontech SMARTer Kit, and Illumina sequencing libraries were prepared with Nextera XT Kit and Nextera Index Kit (Illumina). .. Libraries from 96 cells were pooled and sequenced on four lanes on Illumina HiSeq2000 using 100 bp paired-end protocol.

other:

Article Title: Impact of library preparation protocols and template quantity on the metagenomic reconstruction of a mock microbial community
Article Snippet: Overall, our current work suggests that satisfactory assembly quality can be achieved using the Nextera XT kit down to 5 picograms of input DNA with little effect on downstream analyses such as extracting genomes from metagenomes (Fig. ).

Polymerase Chain Reaction:

Article Title: Tn5 transposase and tagmentation procedures for massively scaled sequencing projects
Article Snippet: .. Elution was performed with 25 μL of resuspension buffer (RSB) from the Nextera kit, and 20 μL was used for the final enrichment PCR along with 15 μL of Nextera PCR master mix (NPM), 5 μL of Index 1 primers (N7xx), 5 μL of Index 2 primers (N5xx), and 5 μL of PCR primer cocktail (PPC; composed of FC-121-1012 and FC-121-1011 primers; see Illumina website). .. The PCR program was as follows: 3 min at 72°C, 30 sec at 98°C, and then five cycles of 10 sec at 98°C, 30 sec at 63°C, and 3 min at 72°C.

Variant Assay:

Article Title: Targeted single molecule mutation detection with massively parallel sequencing
Article Snippet: .. Sequencing specific S288C laboratory strain In order to establish a highly accurate reference sequence for variant calling, the specific strain of S288C used in this study was sequenced using the Nextera XT kit (Illumina). .. Input DNA was taken from the untreated yeast grown on the same day as the mutagenesis experiment in order to ensure all spontaneous mutations occurring during culture were sampled.

Chromatin Immunoprecipitation:

Article Title: Flipping between Polycomb repressed and active transcriptional states introduces noise in gene expression
Article Snippet: .. OS25 cell suspension was loaded on 10–17 micron C1 Single-Cell Auto Prep IFC, Fluidigm; cDNA was synthesized in the chip using Clontech SMARTer Kit, and Illumina sequencing libraries were prepared with Nextera XT Kit and Nextera Index Kit (Illumina). .. Libraries from 96 cells were pooled and sequenced on four lanes on Illumina HiSeq2000 using 100 bp paired-end protocol.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Illumina Inc nextera xt library preparation protocol
    Nextera Xt Library Preparation Protocol, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextera xt library preparation protocol/product/Illumina Inc
    Average 99 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    nextera xt library preparation protocol - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results