nextal dwblock pegs suite  (Qiagen)


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    Name:
    NeXtal DWBlock PEGs Suite
    Description:
    For manual or automated setup of protein crystallization screening conditions Kit contents Piercable deep well block containing 96 x 1 5 ml PEG Suite solution piercing tool adhesive foil Benefits 1 5 ml solution of each Screening Suite condition in a 96 well format For manual or automated setup in microplates Heat sealed pierceable foil ensures optimal storage and shipping Maximized reproducibility through online access to production repor
    Catalog Number:
    130904
    Price:
    359
    Category:
    NeXtal DWBlock Suites
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    Structured Review

    Qiagen nextal dwblock pegs suite
    NeXtal DWBlock PEGs Suite
    For manual or automated setup of protein crystallization screening conditions Kit contents Piercable deep well block containing 96 x 1 5 ml PEG Suite solution piercing tool adhesive foil Benefits 1 5 ml solution of each Screening Suite condition in a 96 well format For manual or automated setup in microplates Heat sealed pierceable foil ensures optimal storage and shipping Maximized reproducibility through online access to production repor
    https://www.bioz.com/result/nextal dwblock pegs suite/product/Qiagen
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nextal dwblock pegs suite - by Bioz Stars, 2020-04
    99/100 stars

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    Clone Assay:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Positron Emission Tomography:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Chromatography:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Size-exclusion Chromatography:

    Article Title: CSL311, a novel, potent, therapeutic monoclonal antibody for the treatment of diseases mediated by the common β chain of the IL-3, GM-CSF and IL-5 receptors
    Article Snippet: Crystallization of the βc /CSL311 Fab complex The βc /CSL311 Fab complex was purified from a mixture of monomeric components by size exclusion chromatography. .. Rectangular plate shaped crystals appeared between 2-3 d in 40% PEG 200 and 100 mM Tris pH 8.5 from the PEGs Suite (Qiagen 130904).

    Purification:

    Article Title: CSL311, a novel, potent, therapeutic monoclonal antibody for the treatment of diseases mediated by the common β chain of the IL-3, GM-CSF and IL-5 receptors
    Article Snippet: Crystallization of the βc /CSL311 Fab complex The βc /CSL311 Fab complex was purified from a mixture of monomeric components by size exclusion chromatography. .. Rectangular plate shaped crystals appeared between 2-3 d in 40% PEG 200 and 100 mM Tris pH 8.5 from the PEGs Suite (Qiagen 130904).

    Concentration Assay:

    Article Title: CSL311, a novel, potent, therapeutic monoclonal antibody for the treatment of diseases mediated by the common β chain of the IL-3, GM-CSF and IL-5 receptors
    Article Snippet: All crystallization trials of the complex were carried out at 18°C using commercially available protein crystallization screens with the protein at a concentration of 6 mg/ml. .. Rectangular plate shaped crystals appeared between 2-3 d in 40% PEG 200 and 100 mM Tris pH 8.5 from the PEGs Suite (Qiagen 130904).

    Expressing:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Crystallization Assay:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Article Title: CSL311, a novel, potent, therapeutic monoclonal antibody for the treatment of diseases mediated by the common β chain of the IL-3, GM-CSF and IL-5 receptors
    Article Snippet: Paragraph title: Crystallization of the βc /CSL311 Fab complex ... Rectangular plate shaped crystals appeared between 2-3 d in 40% PEG 200 and 100 mM Tris pH 8.5 from the PEGs Suite (Qiagen 130904).

    Article Title: Glycosylation of MUC1 influences the binding of a therapeutic antibody by altering the conformational equilibrium of the antigen
    Article Snippet: Paragraph title: Crystallization of AR20.5 Fab in complex with MUC1 peptide and glycopeptides ... AR20.5 Fab was concentrated to 13 mg/mL and mixed with either peptide or glycopeptide (1 mM) and screened against the Nextal DWBlock PEGs, PEG II screens (Qiagen Inc., Valencia, CA) using a CrystalGryphon (Art Robbins Inc., Sunnyvale, CA).

    SDS Page:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

    Plasmid Preparation:

    Article Title: A general protocol for the crystallization of membrane proteins for X-ray structural investigation
    Article Snippet: .. Complete protease inhibitors, EDTA free (Roche, cat. no. 11 873 580 001) Coomassie Brilliant Blue R (Sigma, cat. no. B7920) Plasmid vectors: pET E. coli T7 expression vectors (Novagen) E. coli XL1-blue cells for cloning (Stratagene, cat. no. 200228) OverExpress C43 (DE3): E. coli cells for membrane protein expression (Lucigen, cat. no. 60345-1) LB (Luria-Bertani Broth) Agar Miller (Fisher Scientific, cat. no. BP1425-2) Antibiotics: e.g., kanamycin sulfate (Shelton Scientific, cat. no. IBO2120), Ampicillin sodium salt (Sigma, cat. no. A9518-100G) LB Broth Miller (EMD, cat. no. 1.10258.5007) IPTG (Anatrace, cat. no. I1003) For SDS-PAGE: 4–20% (wt/vol) Tris-glycine gel, 1 mm × 15 well (Invitrogen, cat. no. EC60255BOX) Invitrolon PVDF filter paper sandwich, 0.45-µm pore size (Invitrogen, cat. no. LC2005) Anti-His antibody (Santa Cruz Biotechnology Inc., cat. no. SC-8036 HRP) SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, cat. no. 34080) Detergents (Anatrace): CHAPS (cat. no. C316), DDM (cat. no. D310), FC-12 (cat. no. F308), LDAO (cat. no. D360), OG (cat. no. O311) Sodium dodecyl sulfate (Bio-Rad, cat. no. 161-0302) Ni-NTA Agarose resin (Qiagen, cat. no. 30250) Econo-Pac 10 DG disposable chromatography column (Bio-Rad, cat. no. 732-2010) Thrombin (Novagen, cat. no. 69671) Benzamidine sepharose 6B (GE Healthcare, cat. no. 17-0568-01) Centrifugal filter devices: Ultra-4 or Ultra-15 series depending on what volume is being concentrated (Amicon) Stirred ultrafiltration cells (Amicon) Spectra/Por molecular porous membrane tubing (Spectrum Laboratories Inc.) Spectra/Por Float-A-Lyzer apparatus for dialysis of milliliters of concentrated protein (Spectrum Laboratories Inc.) Micro DispoDialyzer apparatus for dialysis of microliters of concentrated protein (Spectrum Laboratories Inc.) Crystallization screens: MemStart (Molecular Dimensions, cat. no. MD1-21USA), MemSys (Molecular Dimensions, cat. no. MD1-25USA), MemGold (Molecular Dimensions, cat. no. MD1-41), CP-Custom-IV (Axygen Biosciences, cat. no. CP-CUSTOM-IV), Classics Suite (Qiagen, cat. no. 130901), PEGs Suite (Qiagen, cat. no. 130904), PEGs II Suite (Qiagen cat. no. 130916), MbClass Suite (Qiagen, cat. no. 130911), MbClass Suite II (Qiagen, cat. no. 130912), Additive Screen (Hampton Research, cat. no. HR2-138), Detergent Screen (Hampton Research, cat. no. HR2-406), Silver Bullets (Hampton Research, cat. no. HR2-096) 96-well flat-bottomed clear polypropylene plates (E & K Scientific, cat. no. EK-25201) Protein crystallization covers for 96-well plates (Grace Bio-Labs, cat. no. 45232) EasyXtal Tool: 24-well crystallization plate (Qiagen, cat. no. 132023) .. Gene Pulser Xcell total system (Bio-Rad 165-2660) EmulsiFlex C3 or C5 (Avestin Inc.) Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad, cat. no. 170-3940) Trans-Blot SD system and Power-Pac HC power supply system (Bio-Rad, cat. no. 170-3848) Innova 44R incubator shaker (New Brunswick Scientific, cat. no. M1282-014) Spinbar, Teflon, Flea Micro (VWR, cat. no. 58948-976) Superdex 200 10/300 GL (GE Healthcare Bio-Sciences, cat. no. 17-5175-01) TSK-GEL G3000SW (Tosoh Bioscience, cat. no. 05103) TSK-GEL G4000SW (Tosoh Bioscience, cat. no. 05104) Guardcolumn SW (Tosoh Bioscience, cat. no. 05371) to be used with TSK-GEL columns Shimadzu modular HPLC system (see EQUIPMENT SETUP) Mosquito crystal nanoliter liquid handler (TTP Labtech) Leica MZ16 stereomicroscope/KL 1500 LCD (Leica Microsystems) PRS-1000 protein review station (Korima Inc.) 0.2-µm filter, Corning Disposable filter system (Fisher Scientific, cat. no. 09-761-40)

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