nextal amso4 suite  (Qiagen)


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    Name:
    NeXtal DWBlock AmSO4 Suite
    Description:
    For manual or automated setup of protein crystallization screening conditions Kit contents Piercable deep well block containing 96 x 1 5 ml AmSO4 Suite solution piercing tool adhesive foil Benefits 1 5 ml solution of each Screening Suite condition in a 96 well format For manual or automated setup in microplates Heat sealed pierceable foil ensures optimal storage and shipping Maximized reproducibility through online access to production repor
    Catalog Number:
    130905
    Price:
    359
    Category:
    NeXtal DWBlock Suites
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    Structured Review

    Qiagen nextal amso4 suite
    NeXtal DWBlock AmSO4 Suite
    For manual or automated setup of protein crystallization screening conditions Kit contents Piercable deep well block containing 96 x 1 5 ml AmSO4 Suite solution piercing tool adhesive foil Benefits 1 5 ml solution of each Screening Suite condition in a 96 well format For manual or automated setup in microplates Heat sealed pierceable foil ensures optimal storage and shipping Maximized reproducibility through online access to production repor
    https://www.bioz.com/result/nextal amso4 suite/product/Qiagen
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    nextal amso4 suite - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Crystallization and preliminary X-ray crystallographic analysis of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA
    Article Snippet: .. Initial screening for crystallization conditions of native Tth Dus, SeMet- Tth Dus and the native Tth Dus–tRNA complex was per­formed using NeXtal Classics Suite, NeXtal JCSG+ Suite, NeXtal AmSO4 Suite, NeXtal Nucleix Suite, NeXtal ProComplex Suite and NeXtal JCSG Core Suite I–IV (Qiagen, Hilden, Germany) by the sitting-drop vapour-diffusion method, in which 1 µl sample and 1 µl reservoir solution were mixed and equilibrated against 100 µl reservoir solution in 96-well CrystalQuick plates (Greiner Bio-One, Kremsmuenster, Austria) at 293 K. After initial crystals had been obtained, the conditions were optimized by changing the pH value of the reservoir solution and the concentration of the precipitant (except for native Tth Dus). ..

    Crystallization Assay:

    Article Title: Structural insight into nucleotide recognition by human death-associated protein kinase
    Article Snippet: .. Crystallization and data collection For the complex of DAPK with ADP (Sigma catalog No. A5410; lot 50H7080) and Mg2+ , a protein solution at 5.5 mg ml−1 in 100 mM Tris pH 8.0 and 0.25 M NaCl was incubated with 1 mM ADP and 10 mM MgCl2 for 45 min at 277 K. Crystallization conditions were determined with 96-­well plate NeXtal AmSO4 suite screens (catalog No. 130905; Qiagen, Valencia, California, USA) using the sitting-drop method for crystallization. ..

    Article Title: Protein stabilization utilizing a redefined codon
    Article Snippet: .. The initial crystallization trial was performed using a commercial screening kit, NeXtal AmSO4 Suite (Qiagen). ..

    Article Title: Crystallization and preliminary X-ray crystallographic analysis of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA
    Article Snippet: .. Initial screening for crystallization conditions of native Tth Dus, SeMet- Tth Dus and the native Tth Dus–tRNA complex was per­formed using NeXtal Classics Suite, NeXtal JCSG+ Suite, NeXtal AmSO4 Suite, NeXtal Nucleix Suite, NeXtal ProComplex Suite and NeXtal JCSG Core Suite I–IV (Qiagen, Hilden, Germany) by the sitting-drop vapour-diffusion method, in which 1 µl sample and 1 µl reservoir solution were mixed and equilibrated against 100 µl reservoir solution in 96-well CrystalQuick plates (Greiner Bio-One, Kremsmuenster, Austria) at 293 K. After initial crystals had been obtained, the conditions were optimized by changing the pH value of the reservoir solution and the concentration of the precipitant (except for native Tth Dus). ..

    Affinity Chromatography:

    Article Title: Protein stabilization utilizing a redefined codon
    Article Snippet: The pooled fractions eluted with 10 mM reduced glutathione from affinity chromatography on an immobilized glutathione column (GE Healthcare Life Sciences) were applied to a Resource Q (1 mL) column, equilibrated with 50 mM Tris-HCl (pH 8.0) buffer containing 0.2 mM TCEP [tris (2-carboxyethyl)phosphine]. .. The initial crystallization trial was performed using a commercial screening kit, NeXtal AmSO4 Suite (Qiagen).

    Incubation:

    Article Title: Structural insight into nucleotide recognition by human death-associated protein kinase
    Article Snippet: .. Crystallization and data collection For the complex of DAPK with ADP (Sigma catalog No. A5410; lot 50H7080) and Mg2+ , a protein solution at 5.5 mg ml−1 in 100 mM Tris pH 8.0 and 0.25 M NaCl was incubated with 1 mM ADP and 10 mM MgCl2 for 45 min at 277 K. Crystallization conditions were determined with 96-­well plate NeXtal AmSO4 suite screens (catalog No. 130905; Qiagen, Valencia, California, USA) using the sitting-drop method for crystallization. ..

    Purification:

    Article Title: Protein stabilization utilizing a redefined codon
    Article Snippet: The variants were further purified using a 0–500 mM NaCl gradient with an AKTA 10 S system (GE Healthcare Life Sciences). .. The initial crystallization trial was performed using a commercial screening kit, NeXtal AmSO4 Suite (Qiagen).

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  • 99
    Qiagen nextal amso4 suite
    Nextal Amso4 Suite, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nextal amso4 suite/product/Qiagen
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    Qiagen amso4 suite
    Amso4 Suite, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amso4 suite/product/Qiagen
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    amso4 suite - by Bioz Stars, 2020-04
    99/100 stars
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