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neutropenic mice thigh infection model  (ATCC)
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ATCC neutropenic mice thigh infection model
Neutropenic Mice Thigh Infection Model, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutropenic gfi1 r412x/ r412x mice  (Jackson Laboratory)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
Neutropenic Gfi1 R412x/ R412x Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutropenic thigh infection model cd-1 mice  (Charles River Laboratories)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
Neutropenic Thigh Infection Model Cd 1 Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutropenic mouse thigh infection model against s aureus atcc 33591  (ATCC)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
Neutropenic Mouse Thigh Infection Model Against S Aureus Atcc 33591, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neutropenic mice  (Taconic Biosciences)
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Taconic Biosciences neutropenic mice
(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
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neutropenic c57bl 6 mice  (ATCC)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
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neutropenic cd-1 female mice  (Envigo)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
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neutropenic cd–1 male mice  (Charles River Laboratories)
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(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of <t>Gfi1</t> +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.
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Image Search Results


(A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.

Journal: bioRxiv

Article Title: Tissue-resident neutrophils serve homeostatic and immunological functions in embryos

doi: 10.1101/2025.06.12.659311

Figure Lengend Snippet: (A) Flow cytometric analyses of neutrophils in the indicated embryonic (E17.5) tissues of Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (B) Flow cytometric analyses of resident macrophage populations in the indicated tissues of adult Gfi1 +/+ (gray), Gfi1 +/mut (light orange), and Gfi1 mut/mut (orange) mice. (C) Volcano plot assessing differential gene expression in testes of Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (D) STRING functional enrichment visualization of the top 10 gene ontology terms related to biological processes enriched in downregulated DEGs in the testes of Gfi1 mut/mut embryos at E17.5. (E) Volcano plot of differential expression of transposable elements (TE) in testes between Gfi1 mut/mut and WT embryos at E17.5 as determined by bulk RNA-sequencing. (F) Analysis of breeding characteristics of Gfi1 +/mut x Gfi1 +/mut crossings compared to crossings of WT animals. Shown are percentage and absolute numbers (parentheses) of observed overall rate of pregnancies, vaginal plugging efficiency, and rate of pregnancies after detection of a vaginal plug. In A, each dot represents liver, blood, a pool of both kidneys, or a pool of both testes from one mouse. In B, each dot represents the liver, one kidney, or one testis from one mouse. Data in A and B are presented as mean ± SD and are from 3-5 independent experiments. Statistical Analysis was performed using Two-way ANOVA with Dunnett’s multiple comparisons test (A and B). FDR, false discover rate corrected p-values, signal, weighted harmonic mean between the observed/expected ratio and -log(FDR), LINE, long interspersed nuclear element; SINE, short interspersed nuclear element; LTR, long terminal repeat.

Article Snippet: Neutropenic Gfi1 R412X/ R412X mice (Muench et al., 2020) were acquired from Jax lab ( Gfi1 em1Hlg /J, 035156).

Techniques: Gene Expression, RNA Sequencing, Functional Assay, Quantitative Proteomics