neutravidin coated 96 well plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher neutravidin coated 96 well plates
    Neutravidin Coated 96 Well Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neutravidin coated 96 well plates/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    neutravidin coated 96 well plates - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.). .. For each round the ability of randomly picked single phage clones to bind to immobilized full-length E2 (FL-E2; purified according to the method described in reference ) was assessed by phage enzyme-linked immunosorbent assay (ELISA) ( ).

    Centrifugation:

    Article Title: Ribosomal Synthesis of Macrocyclic Peptides in Vitro and in Vivo Mediated by Genetically Encoded Amino-Thiol Unnatural Amino Acids
    Article Snippet: After harvesting, cells were re-suspended in 400 µL of TBS (Tris 50 mM, NaCl 150 mM, 7.4), and incubated for 1 h at 37 °C in the presence of lysosyme, DNase, and MgCl2 to lyse the cells, followed by centrifugation at 3,400 g . .. The clarified cell lysate (200 µL) was transferred to NeutrAvidin coated 96-well plates (Pierce) and incubated for 2 h at room temperature.

    Synthesized:

    Article Title: Hexose Potentiates Peptide-Conjugated Morpholino Oligomer Efficacy in Cardiac Muscles of Dystrophic Mice in an Age-Dependent Manner
    Article Snippet: Briefly, a DNA probe was designed with sequences complementary to PMO (synthesized by The Beijing Genomics Institute, Beijing, China) as follows: 5′-ATTTCAGGTAAGCCGAGGTTTGGCC-3′, with phosphorothioate ends in bold. .. Following PMO-probe hybridization, the avidin-biotin interaction of the hybridized probe was performed on Pierce NeutrAvidin Coated 96-well plates, Black (Thermo Fisher Scientific, MA, USA).

    Blocking Assay:

    Article Title: Phase I study of GTI-2040, a ribonucleotide reductase antisense, with high dose cytarabine in patients with relapsed/refractory acute myeloid leukemia
    Article Snippet: Samples were added to NeutrAvidin-coated 96-well plates (Pierce Co., Rockford, IL), incubated at 42°C for 2 h and subsequently washed with buffer (Tris-buffered saline [TBS] in 0.1% Tween 20). .. The plate was washed with buffer, and anti-digoxigenin-alkaline phosphatase (1:2500 with bovine serum albumin block buffer in TBS; Roche, Indianapolis, IN) was added.

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce). .. Subsequently, 100 μL of a solution containing 5.4% glycerol, 32.3 mM NaAc, 1.1 mM ZnAc, 650 mM NaCl, and 326 units/mL of nuclease S1 was added to each well and the plates were covered and shaken carefully before the solutions were allowed to incubate at r.t. for 2 h. Again the plates were washed three times with stringency buffer as described above, before 100 μL of a solution containing 0.5 μL of Anti-Digoxigenin-AP (150 U/200 μL, Roche) in 10% Super Block® blocking buffer (Pierce) was added to each well.

    Article Title: Development of Highly Selective 1,2,3-Triazole-containing Peptidic Polo-like Kinase 1 Polo-box Domain-binding Inhibitors
    Article Snippet: Determination of Inhibitory Potency in an ELISA Assay Using Full-Length Plk1 As previously reported [ , , ] a biotinylated phosphopeptide (sequence: Biotin-Ahx-PMQS(pT)PLN-NH2 ) was diluted to 1 µM (from a 2 mM DMSO stock solution) in PBS (pH 7.4) and loaded onto the wells of a 96-well Neutravidin-coated plate (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA, USA) at 100 µL per well for 1 h (background control contained no biotinylated peptide). .. The wells were washed once with 150 µL PBST (PBS, pH 7.4 + 0.05% Tween-20), and then 100 µL of 1% BSA in PBS (pH 7.4) (blocking buffer) was added for 1 h. A cytosolic lysate-containing transiently expressed myc-tagged Plk1 protein was diluted to 300 µg/mL in PBS (pH 7.4) containing protease/phosphatase inhibitors (Pierce Biotechnology), mixed with competitive inhibitor (from a 10× stock in ~4% DMSO/PBS), and allowed to pre-incubate for 1 h (100 µL per well in a 96-well plate, 30 µg total protein).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Phase I study of GTI-2040, a ribonucleotide reductase antisense, with high dose cytarabine in patients with relapsed/refractory acute myeloid leukemia
    Article Snippet: A novel hybridization-ligation based enzyme-linked immunosorbent assay (ELISA) was developed and validated in our laboratory for the measurement of GTI-2040 levels in plasma and lysates from bone marrow (BM) mononuclear cells (MNCs) [ , ]. .. Samples were added to NeutrAvidin-coated 96-well plates (Pierce Co., Rockford, IL), incubated at 42°C for 2 h and subsequently washed with buffer (Tris-buffered saline [TBS] in 0.1% Tween 20).

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.). .. For each round the ability of randomly picked single phage clones to bind to immobilized full-length E2 (FL-E2; purified according to the method described in reference ) was assessed by phage enzyme-linked immunosorbent assay (ELISA) ( ).

    Article Title: Human Monoclonal Antibodies to Sialyl-Lewis a (CA19.9) with Potent CDC, ADCC and Anti-Tumor Activity
    Article Snippet: .. For the sLea ELISA, plates were coated either with 1 μg/ml of sLea -HSA conjugate, monovalent biotinylated-sLea , or with polyvalent biotinylated sLea -PAA captured on Neutravidin (Pierce, Rockford, IL) coated plates. ..

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Paragraph title: ELISA-based assay for PNA quantitation in tissue samples ... Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature. .. Plates were washed in six cycles with HNT buffer on the ELx405, and then a horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibody (Jackson Immunologicals) diluted 1:10,000 in HNT buffer was added and incubated for 1 h. After a final series of identical washes, 50 μl of Super Signal ELISA (enzyme-linked immunosorbent assay) Fempto chemiluminescent substrate (Pierce) was added, and luminescence signals were measured on a Perkin Elmer Envision reader.

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: Paragraph title: ELISA. ... Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific).

    Article Title:
    Article Snippet: Paragraph title: ELISA for Human Plasma ... For biotinylated antigens, Neutravidin-coated plates (Pierce) were used instead of Streptavidin-coated plates because the latter gave high background responses with the HIV-1 plasmas.

    Article Title: Development of Highly Selective 1,2,3-Triazole-containing Peptidic Polo-like Kinase 1 Polo-box Domain-binding Inhibitors
    Article Snippet: .. Determination of Inhibitory Potency in an ELISA Assay Using Full-Length Plk1 As previously reported [ , , ] a biotinylated phosphopeptide (sequence: Biotin-Ahx-PMQS(pT)PLN-NH2 ) was diluted to 1 µM (from a 2 mM DMSO stock solution) in PBS (pH 7.4) and loaded onto the wells of a 96-well Neutravidin-coated plate (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA, USA) at 100 µL per well for 1 h (background control contained no biotinylated peptide). .. The wells were washed once with 150 µL PBST (PBS, pH 7.4 + 0.05% Tween-20), and then 100 µL of 1% BSA in PBS (pH 7.4) (blocking buffer) was added for 1 h. A cytosolic lysate-containing transiently expressed myc-tagged Plk1 protein was diluted to 300 µg/mL in PBS (pH 7.4) containing protease/phosphatase inhibitors (Pierce Biotechnology), mixed with competitive inhibitor (from a 10× stock in ~4% DMSO/PBS), and allowed to pre-incubate for 1 h (100 µL per well in a 96-well plate, 30 µg total protein).

    Article Title: CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation
    Article Snippet: .. ELISA ELISA was performed with NeutrAvidin coated 96-well plates (Pierce) and biotinylated peptides. .. Pre-blocked plates were washed three times with ELISA wash buffer (TBS-T with 0.1% [wt/vol] BSA).

    Article Title: Hexose Potentiates Peptide-Conjugated Morpholino Oligomer Efficacy in Cardiac Muscles of Dystrophic Mice in an Age-Dependent Manner
    Article Snippet: Paragraph title: ELISA for PMO ... Following PMO-probe hybridization, the avidin-biotin interaction of the hybridized probe was performed on Pierce NeutrAvidin Coated 96-well plates, Black (Thermo Fisher Scientific, MA, USA).

    Quantitation Assay:

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Paragraph title: ELISA-based assay for PNA quantitation in tissue samples ... Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Activity Assay:

    Article Title:
    Article Snippet: Plasma from a well characterized panel of HIV-1-infected subjects with documented broadly neutralizing antibody activity ( ) and plasma from 10 control subjects were tested for binding to biotinylated haptens, 5-helix, and control antigens. .. For biotinylated antigens, Neutravidin-coated plates (Pierce) were used instead of Streptavidin-coated plates because the latter gave high background responses with the HIV-1 plasmas.

    Modification:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Dual Bbs I sites were then engineered into the modified fTC vector by using Xho I- and Kpn I-digested fTC, and the Xho I site was removed by site-directed mutagenesis, yielding the phage display vector fGW. fGW was digested with Bbs I and ligated with a DNA cassette encoding a 30-amino-acid sequence containing a 10-residue stretch of random amino acids (NH2 -EDGGSXXXXXXXXXXGGGGSGGGGSGGGGS, where X represents any of the 20 common amino acids). .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Transformation Assay:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Based on transformation efficiency and sequencing of primary transformants, the library was estimated to contain about 1010 different peptide sequences. .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Splicing Assay:

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: Paragraph title: High-throughput in vitro splicing assay in 384-well plates. ... After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature.

    Hybridization:

    Article Title: Phase I study of GTI-2040, a ribonucleotide reductase antisense, with high dose cytarabine in patients with relapsed/refractory acute myeloid leukemia
    Article Snippet: A novel hybridization-ligation based enzyme-linked immunosorbent assay (ELISA) was developed and validated in our laboratory for the measurement of GTI-2040 levels in plasma and lysates from bone marrow (BM) mononuclear cells (MNCs) [ , ]. .. Samples were added to NeutrAvidin-coated 96-well plates (Pierce Co., Rockford, IL), incubated at 42°C for 2 h and subsequently washed with buffer (Tris-buffered saline [TBS] in 0.1% Tween 20).

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Hybridization to the cutting probe with a sequence complementary to the analyte (TGTAGATGTGCTGAGA), which was 5′-modified with digoxigenin spaced via an hexylaminolinker and 3′-modified with biotin spaced via triethylene glycol linker, was carried out in Axygen 96 well PCR plates as follows: To 60 μL of each analyte solution per well was added 60 μL of hybridization buffer (24 mM Tris, 600 mM NaCl, 26.4 mM MgCl, 1.2% SDS, pH 9.1) containing 200 nM of the cutting probe and 200 nM of a non-sequence-related oligonucleotide to prevent non-specific binding. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: .. Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific). .. Unhybridized probes were digested with micrococcal nuclease at 0.1 gel unit/μL (New England Biolabs).

    Article Title: Hexose Potentiates Peptide-Conjugated Morpholino Oligomer Efficacy in Cardiac Muscles of Dystrophic Mice in an Age-Dependent Manner
    Article Snippet: .. Following PMO-probe hybridization, the avidin-biotin interaction of the hybridized probe was performed on Pierce NeutrAvidin Coated 96-well plates, Black (Thermo Fisher Scientific, MA, USA). .. Unhybridized probes were digested with micrococcal nuclease at 10 U/μL (Thermo Fisher Scientific, MA, USA).

    Ligation:

    Article Title: Phase I study of GTI-2040, a ribonucleotide reductase antisense, with high dose cytarabine in patients with relapsed/refractory acute myeloid leukemia
    Article Snippet: Samples were added to NeutrAvidin-coated 96-well plates (Pierce Co., Rockford, IL), incubated at 42°C for 2 h and subsequently washed with buffer (Tris-buffered saline [TBS] in 0.1% Tween 20). .. Next, a detection ODN probe (5′-CACTAGTTA-3′) with phosphate at the 5′-end and digoxigenin at the 3′-end was diluted in ligation buffer (66 mmol/L Tris-HCl [pH 7.6], 10 mmol/L MgCl2 , 10 mmol/L dithiothreitol [DTT], 1 mmol/L adenosine triphosphate [ATP]) containing 5 U/mL T4 DNA ligase (Amersham Biosciences, Piscataway, NJ) and incubated overnight at 18°C.

    Library Screening:

    Article Title: Ribosomal Synthesis of Macrocyclic Peptides in Vitro and in Vivo Mediated by Genetically Encoded Amino-Thiol Unnatural Amino Acids
    Article Snippet: Paragraph title: Library Screening ... The clarified cell lysate (200 µL) was transferred to NeutrAvidin coated 96-well plates (Pierce) and incubated for 2 h at room temperature.

    Sequencing:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Based on transformation efficiency and sequencing of primary transformants, the library was estimated to contain about 1010 different peptide sequences. .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Hybridization to the cutting probe with a sequence complementary to the analyte (TGTAGATGTGCTGAGA), which was 5′-modified with digoxigenin spaced via an hexylaminolinker and 3′-modified with biotin spaced via triethylene glycol linker, was carried out in Axygen 96 well PCR plates as follows: To 60 μL of each analyte solution per well was added 60 μL of hybridization buffer (24 mM Tris, 600 mM NaCl, 26.4 mM MgCl, 1.2% SDS, pH 9.1) containing 200 nM of the cutting probe and 200 nM of a non-sequence-related oligonucleotide to prevent non-specific binding. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: Development of Highly Selective 1,2,3-Triazole-containing Peptidic Polo-like Kinase 1 Polo-box Domain-binding Inhibitors
    Article Snippet: .. Determination of Inhibitory Potency in an ELISA Assay Using Full-Length Plk1 As previously reported [ , , ] a biotinylated phosphopeptide (sequence: Biotin-Ahx-PMQS(pT)PLN-NH2 ) was diluted to 1 µM (from a 2 mM DMSO stock solution) in PBS (pH 7.4) and loaded onto the wells of a 96-well Neutravidin-coated plate (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA, USA) at 100 µL per well for 1 h (background control contained no biotinylated peptide). .. The wells were washed once with 150 µL PBST (PBS, pH 7.4 + 0.05% Tween-20), and then 100 µL of 1% BSA in PBS (pH 7.4) (blocking buffer) was added for 1 h. A cytosolic lysate-containing transiently expressed myc-tagged Plk1 protein was diluted to 300 µg/mL in PBS (pH 7.4) containing protease/phosphatase inhibitors (Pierce Biotechnology), mixed with competitive inhibitor (from a 10× stock in ~4% DMSO/PBS), and allowed to pre-incubate for 1 h (100 µL per well in a 96-well plate, 30 µg total protein).

    Binding Assay:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Paragraph title: Phage display selection of E2-DNA binding inhibitors. ... A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Hybridization to the cutting probe with a sequence complementary to the analyte (TGTAGATGTGCTGAGA), which was 5′-modified with digoxigenin spaced via an hexylaminolinker and 3′-modified with biotin spaced via triethylene glycol linker, was carried out in Axygen 96 well PCR plates as follows: To 60 μL of each analyte solution per well was added 60 μL of hybridization buffer (24 mM Tris, 600 mM NaCl, 26.4 mM MgCl, 1.2% SDS, pH 9.1) containing 200 nM of the cutting probe and 200 nM of a non-sequence-related oligonucleotide to prevent non-specific binding. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: Increased Aβ42-α7-like nicotinic acetylcholine receptor complex level in lymphocytes is associated with apolipoprotein E4-driven Alzheimer’s disease pathogenesis
    Article Snippet: .. In vitro assessment of Aβ42 -α7nAChR and Aβ42 -Aβ42 interaction The effect of apoE fragments and ApoE isoform on Aβ42 -α7nAChR interaction was measured in vitro with 2 nM biotinated α7nAChRs trapped on streptavidin-coated plate (Reacti-Bind™ NeutrAvidin™ High binding capacity coated 96-well plate; Pierce). .. Biotinylation of the cell surface proteins was performed using the Pierce cell surface protein isolation kit according to the manufacturer’s protocol.

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: Nucleotides from the 5′ and 3′ ends of the probe DNA sequences were phosphorothioated to increase the stability of probe-target binding ( ). .. Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific).

    Article Title:
    Article Snippet: Plasma from a well characterized panel of HIV-1-infected subjects with documented broadly neutralizing antibody activity ( ) and plasma from 10 control subjects were tested for binding to biotinylated haptens, 5-helix, and control antigens. .. For biotinylated antigens, Neutravidin-coated plates (Pierce) were used instead of Streptavidin-coated plates because the latter gave high background responses with the HIV-1 plasmas.

    Article Title: CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation
    Article Snippet: ELISA ELISA was performed with NeutrAvidin coated 96-well plates (Pierce) and biotinylated peptides. .. Peptide binding was achieved at room temperature after 2 h. Unbound peptides were removed by washing three times with ELISA wash buffer, and decreasing dilutions of test antibodies were transferred to the wells for 20 min. Wells were washed three times, and secondary HRP-conjugated antibodies were added for 20 min. Wells were washed again three times, and substrate solution (110 mM sodium acetate, pH 5.5; 0.1% [vol/vol] H2 O2 ; and 0.1 µg/ml TMB) was added for 5 min.

    Fluorescence:

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific). .. Fluorescence signals from the PPMO-hybridized probes were detected at 444 nm excitation and 555 nm emission with AttoPhos AP Fluorescent Substrate (Promega) in a monochromator SpectraMax M3 plate reader (Molecular Devices).

    Mutagenesis:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Dual Bbs I sites were then engineered into the modified fTC vector by using Xho I- and Kpn I-digested fTC, and the Xho I site was removed by site-directed mutagenesis, yielding the phage display vector fGW. fGW was digested with Bbs I and ligated with a DNA cassette encoding a 30-amino-acid sequence containing a 10-residue stretch of random amino acids (NH2 -EDGGSXXXXXXXXXXGGGGSGGGGSGGGGS, where X represents any of the 20 common amino acids). .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Isolation:

    Article Title: Increased Aβ42-α7-like nicotinic acetylcholine receptor complex level in lymphocytes is associated with apolipoprotein E4-driven Alzheimer’s disease pathogenesis
    Article Snippet: In vitro assessment of Aβ42 -α7nAChR and Aβ42 -Aβ42 interaction The effect of apoE fragments and ApoE isoform on Aβ42 -α7nAChR interaction was measured in vitro with 2 nM biotinated α7nAChRs trapped on streptavidin-coated plate (Reacti-Bind™ NeutrAvidin™ High binding capacity coated 96-well plate; Pierce). .. Biotinylation of the cell surface proteins was performed using the Pierce cell surface protein isolation kit according to the manufacturer’s protocol.

    Avidin-Biotin Assay:

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: .. Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific). .. Unhybridized probes were digested with micrococcal nuclease at 0.1 gel unit/μL (New England Biolabs).

    Article Title: Hexose Potentiates Peptide-Conjugated Morpholino Oligomer Efficacy in Cardiac Muscles of Dystrophic Mice in an Age-Dependent Manner
    Article Snippet: .. Following PMO-probe hybridization, the avidin-biotin interaction of the hybridized probe was performed on Pierce NeutrAvidin Coated 96-well plates, Black (Thermo Fisher Scientific, MA, USA). .. Unhybridized probes were digested with micrococcal nuclease at 10 U/μL (Thermo Fisher Scientific, MA, USA).

    Labeling:

    Article Title: Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy
    Article Snippet: In brief, cDNA probes (Integrated DNA Technologies) that bind to the SSO sequences of dog Ex6A, Ex6B, and Ex8A PPMOs were prepared as follows: The 5′ and 3′ ends of the probes were labeled with digoxigenin and biotin, respectively. .. Following PPMO-probe hybridization, the avidin–biotin interaction of the hybridized cocktail probes was performed on Pierce NeutrAvidin Coated 96-Well Plates, Black (Thermo Fisher Scientific).

    Article Title: Hexose Potentiates Peptide-Conjugated Morpholino Oligomer Efficacy in Cardiac Muscles of Dystrophic Mice in an Age-Dependent Manner
    Article Snippet: The 5′ and 3′ ends of the probe were labeled with digoxigenin and biotin, respectively. .. Following PMO-probe hybridization, the avidin-biotin interaction of the hybridized probe was performed on Pierce NeutrAvidin Coated 96-well plates, Black (Thermo Fisher Scientific, MA, USA).

    Purification:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.). ..

    Polymerase Chain Reaction:

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: Hybridization to the cutting probe with a sequence complementary to the analyte (TGTAGATGTGCTGAGA), which was 5′-modified with digoxigenin spaced via an hexylaminolinker and 3′-modified with biotin spaced via triethylene glycol linker, was carried out in Axygen 96 well PCR plates as follows: To 60 μL of each analyte solution per well was added 60 μL of hybridization buffer (24 mM Tris, 600 mM NaCl, 26.4 mM MgCl, 1.2% SDS, pH 9.1) containing 200 nM of the cutting probe and 200 nM of a non-sequence-related oligonucleotide to prevent non-specific binding. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Construct:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: The DNA cassette encoding the 10-mer random peptide sequences was constructed by a synthetic ligase chain reaction procedure ( ). .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Plasmid Preparation:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Dual Bbs I sites were then engineered into the modified fTC vector by using Xho I- and Kpn I-digested fTC, and the Xho I site was removed by site-directed mutagenesis, yielding the phage display vector fGW. fGW was digested with Bbs I and ligated with a DNA cassette encoding a 30-amino-acid sequence containing a 10-residue stretch of random amino acids (NH2 -EDGGSXXXXXXXXXXGGGGSGGGGSGGGGS, where X represents any of the 20 common amino acids). .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    Selection:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: Paragraph title: Phage display selection of E2-DNA binding inhibitors. ... A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    In Vitro:

    Article Title: Increased Aβ42-α7-like nicotinic acetylcholine receptor complex level in lymphocytes is associated with apolipoprotein E4-driven Alzheimer’s disease pathogenesis
    Article Snippet: .. In vitro assessment of Aβ42 -α7nAChR and Aβ42 -Aβ42 interaction The effect of apoE fragments and ApoE isoform on Aβ42 -α7nAChR interaction was measured in vitro with 2 nM biotinated α7nAChRs trapped on streptavidin-coated plate (Reacti-Bind™ NeutrAvidin™ High binding capacity coated 96-well plate; Pierce). .. Biotinylation of the cell surface proteins was performed using the Pierce cell surface protein isolation kit according to the manufacturer’s protocol.

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: Paragraph title: High-throughput in vitro splicing assay in 384-well plates. ... After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature.

    Incubation:

    Article Title: Phase I study of GTI-2040, a ribonucleotide reductase antisense, with high dose cytarabine in patients with relapsed/refractory acute myeloid leukemia
    Article Snippet: .. Samples were added to NeutrAvidin-coated 96-well plates (Pierce Co., Rockford, IL), incubated at 42°C for 2 h and subsequently washed with buffer (Tris-buffered saline [TBS] in 0.1% Tween 20). .. Next, a detection ODN probe (5′-CACTAGTTA-3′) with phosphate at the 5′-end and digoxigenin at the 3′-end was diluted in ligation buffer (66 mmol/L Tris-HCl [pH 7.6], 10 mmol/L MgCl2 , 10 mmol/L dithiothreitol [DTT], 1 mmol/L adenosine triphosphate [ATP]) containing 5 U/mL T4 DNA ligase (Amersham Biosciences, Piscataway, NJ) and incubated overnight at 18°C.

    Article Title: Ribosomal Synthesis of Macrocyclic Peptides in Vitro and in Vivo Mediated by Genetically Encoded Amino-Thiol Unnatural Amino Acids
    Article Snippet: .. The clarified cell lysate (200 µL) was transferred to NeutrAvidin coated 96-well plates (Pierce) and incubated for 2 h at room temperature. ..

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: The plates were sealed with aluminum foil adhesive and shaken carefully before they were heated to 75 °C for 5 min and incubated overnight at 37 °C. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: .. After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature. .. Samples were aspirated using an ELx405 microplate washer (Bio-Tek), and 40 μl of primary anti-eIF4AIII (3F1) antibody ( ) diluted 1:350 in HNT buffer with 1 μg/ml bovine serum albumin (BSA) was added to each well of the plate using a Thermo Multidrop and incubated for 1 h at room temperature.

    Article Title:
    Article Snippet: For biotinylated antigens, Neutravidin-coated plates (Pierce) were used instead of Streptavidin-coated plates because the latter gave high background responses with the HIV-1 plasmas. .. Neutravidin plates were washed three times, and then 50 μl of test antigens at a concentration of 2 μg/ml (or 4 μg/ml for HA022B) was added and incubated overnight at 4 °C.

    Article Title: Development of Highly Selective 1,2,3-Triazole-containing Peptidic Polo-like Kinase 1 Polo-box Domain-binding Inhibitors
    Article Snippet: Determination of Inhibitory Potency in an ELISA Assay Using Full-Length Plk1 As previously reported [ , , ] a biotinylated phosphopeptide (sequence: Biotin-Ahx-PMQS(pT)PLN-NH2 ) was diluted to 1 µM (from a 2 mM DMSO stock solution) in PBS (pH 7.4) and loaded onto the wells of a 96-well Neutravidin-coated plate (Pierce Biotechnology, ThermoFisher Scientific, Waltham, MA, USA) at 100 µL per well for 1 h (background control contained no biotinylated peptide). .. The blocked ELISA plate was washed 2× with PBST (PBS, pH 7.4 + 0.05% Tween-20) (150 µL) and the pre-incubated lysates were added to the plate and incubated (1 h).

    Concentration Assay:

    Article Title: Peptide Nucleic Acids Conjugated to Short Basic Peptides Show Improved Pharmacokinetics and Antisense Activity in Adipose Tissue
    Article Snippet: On the same plate, a concentration ladder was prepared from standard solutions for each analyte with final concentrations of 100 nM, 40 nM, 10 nM, 4 nM, 1 nM and 0.1 nM as described above. .. Using a multichannel pipettor, 100 μL of each well were transferred to its respective well on a clear NeutrAvidin™ -coated 96-well plate (Pierce).

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: A Beckman Coulter Biomek FX robot was used to assemble 20-μl splicing reaction mixtures consisting of 1× SP buffer (0.5 mM ATP, 20 mM creatine phosphate, 1.6 mM MgCl2 ), 80 μg of whole-cell splicing extract, 40 nM biotin-labeled Ad2ΔIVS pre-mRNA, 1 U/μl of RNasin RNase inhibitor (Promega), and 20 μM each individual compound (final concentration, 1% dimethyl sulfoxide [DMSO]). .. After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature.

    Article Title:
    Article Snippet: For biotinylated antigens, Neutravidin-coated plates (Pierce) were used instead of Streptavidin-coated plates because the latter gave high background responses with the HIV-1 plasmas. .. Neutravidin plates were washed three times, and then 50 μl of test antigens at a concentration of 2 μg/ml (or 4 μg/ml for HA022B) was added and incubated overnight at 4 °C.

    FLAG-tag:

    Article Title: Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein
    Article Snippet: DNA sequence encoding a Flag tag (AspTyrLysAspAspAspLys) and Xho I and Kpn I restriction sites was inserted into the vector fTC ( ). .. A total of 2 × 1011 fGWX10 phage CFU in 100 μl of TBSDM buffer (25 mM Tris-HCl [pH 7.0], 150 mM NaCl, 5 mM dithiothreitol [DTT], 2 mM MgCl2 ) containing 0.5% milk was added to wells containing purified bio-E2C captured on NeutrAvidin-coated 96-well plates (Pierce, Rockford, Ill.).

    High Throughput Screening Assay:

    Article Title: A Quantitative High-Throughput In Vitro Splicing Assay Identifies Inhibitors of Spliceosome Catalysis
    Article Snippet: Paragraph title: High-throughput in vitro splicing assay in 384-well plates. ... After 1.5 h at 30°C, reaction mixtures were diluted with 40 μl of HNT buffer (20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 0.5% Triton X-100), and 50 μl was transferred to black-well NeutrAvidin-coated plates (Pierce) for a 1-h incubation at room temperature.

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  • 93
    Thermo Fisher 96 well neutravidin coated plate
    96 Well Neutravidin Coated Plate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96 well neutravidin coated plate - by Bioz Stars, 2020-04
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