Journal: PLoS ONE
Article Title: Long-Term Fish Oil Supplementation Induces Cardiac Electrical Remodeling by Changing Channel Protein Expression in the Rabbit Model
doi: 10.1371/journal.pone.0010140
Figure Lengend Snippet: ( A ) Immunoblot images of Kv4.2, Kv4.3, and KChIP2 (∼80 ug/lane). ( B ) Immunoblot images of Kv1.4 (∼160 ug/lane). Size marker bands (in kDa) are listed on the left. Sizes for proteins of interest are marked on the right. Note that for panel (A) here and , each channel subunit immunoblot was corrected by its own α-actin immunoblot for loading variations, although only one representative α-actin immunoblot is shown. Loading variation in Fig. 6B was further checked by coomassie blue (CB) stain. ( C ) Data summary: background-subtracted band intensities were divided by corresponding α-actin band intensity, and then normalized by the mean value of control lanes.
Article Snippet: After fractionation, the proteins were blotted to PVDF membranes (Amersham), and probed with the following antibodies: Cav1.2 mAb (NeuroMabs), Cav1.1 mAb (Abcam), Kv4.2 pAb (Sigma), Kv4.3 pAb (Alomone), KChIP2 mAb (NeuroMabs), Kv1.4 mAb (NeuroMabs) and hERG pAb (Alomone).
Techniques: Western Blot, Marker, Staining