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A, B , confocal fluorescence micrographs of retinal slices showing a brightly labelled AII amacrine cell dialyzed with <t>Neurobiotin</t> and AII amacrine cells and bipolar cells coupled with the dialyzed cell. Images are Z‐stacks of confocal sections showing all labelled cells in the retinal slice. Scale bars, 50 µm. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. A , control solution. Several AII amacrine cells, as well as bipolar cells, are coupled with the dialyzed AII cell. B , NMDA + d ‐serine. No other AII amacrine cells are coupled with the dialyzed AII cell in this image. C , summary bar graph showing a NMDA + d ‐serine‐mediated decrease in coupling between AII amacrine cells. P ‐values are calculated using one‐way ANOVA followed by Tukey‐Kramer post hoc multiple comparisons.
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A, B , confocal fluorescence micrographs of retinal slices showing a brightly labelled AII amacrine cell dialyzed with Neurobiotin and AII amacrine cells and bipolar cells coupled with the dialyzed cell. Images are Z‐stacks of confocal sections showing all labelled cells in the retinal slice. Scale bars, 50 µm. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. A , control solution. Several AII amacrine cells, as well as bipolar cells, are coupled with the dialyzed AII cell. B , NMDA + d ‐serine. No other AII amacrine cells are coupled with the dialyzed AII cell in this image. C , summary bar graph showing a NMDA + d ‐serine‐mediated decrease in coupling between AII amacrine cells. P ‐values are calculated using one‐way ANOVA followed by Tukey‐Kramer post hoc multiple comparisons.

Journal: The Journal of Physiology

Article Title: Junctional conductance of retinal AII amacrine cell electrical synapses is decreased by NMDA receptors

doi: 10.1113/JP286537

Figure Lengend Snippet: A, B , confocal fluorescence micrographs of retinal slices showing a brightly labelled AII amacrine cell dialyzed with Neurobiotin and AII amacrine cells and bipolar cells coupled with the dialyzed cell. Images are Z‐stacks of confocal sections showing all labelled cells in the retinal slice. Scale bars, 50 µm. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. A , control solution. Several AII amacrine cells, as well as bipolar cells, are coupled with the dialyzed AII cell. B , NMDA + d ‐serine. No other AII amacrine cells are coupled with the dialyzed AII cell in this image. C , summary bar graph showing a NMDA + d ‐serine‐mediated decrease in coupling between AII amacrine cells. P ‐values are calculated using one‐way ANOVA followed by Tukey‐Kramer post hoc multiple comparisons.

Article Snippet: Borosilicate micropipettes (5–8 MΩ) contained a K + ‐based internal solution using Alexa Fluor 488 hydrazide and 0.4% Neurobiotin Tracer (Vector labs, #SP‐1120‐50).

Techniques: Fluorescence, Control