nebnext ultra ii end repair da tailing module  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra II End Repair dA Tailing Module
    Description:
    NEBNext Ultra II End Repair dA Tailing Module 96 rxns
    Catalog Number:
    e7546l
    Price:
    795
    Size:
    96 rxns
    Category:
    DNA Template Preparation for PCR
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    Structured Review

    New England Biolabs nebnext ultra ii end repair da tailing module
    NEBNext Ultra II End Repair dA Tailing Module
    NEBNext Ultra II End Repair dA Tailing Module 96 rxns
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Construct:

    Article Title: The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo
    Article Snippet: .. ChIP-seq Libraries were constructed by end repair and A-tailing using the NEB End Repair Module (cat. # E7546S). .. Illumina adaptors were ligated to repaired DNA molecules using T4 ligase (NEB; cat. # M0202S).

    Purification:

    Article Title: Identification of potential regulatory mutations using multi-omics analysis and haplotyping of lung adenocarcinoma cell lines
    Article Snippet: .. End-prep was performed using a NEBNext End repair/dA-tailing Module (E7546, NEB) and end-prepped DNAs were purified using Agencourt AMPure XP beads (Beckman Coulter). .. Adapter ligation and tether attachment were conducted using the NEBNext Blunt/TA Ligase Master Mix (M0367S, NEB) and Ligation Sequencing Kit SQK-LSK208 for 2D, SQK-LSK108 for 1D and SQK-LSK308 for 1D2 .

    Article Title: Integrative transcriptome sequencing reveals extensive alternative trans-splicing and cis-backsplicing in human cells
    Article Snippet: .. Briefly, 1ug purified amplicons were end repaired and A tailed using NEBNext Ultra II End Repair/dA-Tailing Module (New England BioLabs). .. Individual barcode was added to the A-tailed amplicons using NEB Blunt / TA Ligase Master Mix (New England BioLabs).

    Incubation:

    Article Title: Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform
    Article Snippet: .. The resulting fragment was mixed with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Cat. E7546L) in a clean 8-well 0.2 mL PCR strip and incubated for 5min at 20° for end-repair reaction, and then 5 min at 65° for dA-tailing reaction. .. 3 conditions were tested to optimize end-repair/dA-tailing: 1) ∼0.5 pmol DNA (∼162.5 ng) in 60uL total reaction mixture; 2) ∼0.5 pmol DNA in 30 µL total reaction mixture with addition of 0.9 µL 100 mM dATP before the 65° incubation; 3) ∼0.5 pmol DNA control fragment in 30 µL reaction mixture with addition of 0.9 µL 100 mM dATP and 1µL Bst 2.0 WarmStart DNA polymerase (NEB, Cat. M0538S) before the 65° incubation.

    Stripping Membranes:

    Article Title: Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform
    Article Snippet: .. The resulting fragment was mixed with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Cat. E7546L) in a clean 8-well 0.2 mL PCR strip and incubated for 5min at 20° for end-repair reaction, and then 5 min at 65° for dA-tailing reaction. .. 3 conditions were tested to optimize end-repair/dA-tailing: 1) ∼0.5 pmol DNA (∼162.5 ng) in 60uL total reaction mixture; 2) ∼0.5 pmol DNA in 30 µL total reaction mixture with addition of 0.9 µL 100 mM dATP before the 65° incubation; 3) ∼0.5 pmol DNA control fragment in 30 µL reaction mixture with addition of 0.9 µL 100 mM dATP and 1µL Bst 2.0 WarmStart DNA polymerase (NEB, Cat. M0538S) before the 65° incubation.

    Polymerase Chain Reaction:

    Article Title: Rapid Multiplex Small DNA Sequencing on the MinION Nanopore Sequencing Platform
    Article Snippet: .. The resulting fragment was mixed with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, Cat. E7546L) in a clean 8-well 0.2 mL PCR strip and incubated for 5min at 20° for end-repair reaction, and then 5 min at 65° for dA-tailing reaction. .. 3 conditions were tested to optimize end-repair/dA-tailing: 1) ∼0.5 pmol DNA (∼162.5 ng) in 60uL total reaction mixture; 2) ∼0.5 pmol DNA in 30 µL total reaction mixture with addition of 0.9 µL 100 mM dATP before the 65° incubation; 3) ∼0.5 pmol DNA control fragment in 30 µL reaction mixture with addition of 0.9 µL 100 mM dATP and 1µL Bst 2.0 WarmStart DNA polymerase (NEB, Cat. M0538S) before the 65° incubation.

    ChIP-sequencing:

    Article Title: The maternal to zygotic transition regulates genome-wide heterochromatin establishment in the zebrafish embryo
    Article Snippet: .. ChIP-seq Libraries were constructed by end repair and A-tailing using the NEB End Repair Module (cat. # E7546S). .. Illumina adaptors were ligated to repaired DNA molecules using T4 ligase (NEB; cat. # M0202S).

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  • Bioz Stars
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  • 93
    New England Biolabs nebnext ultra ii end repair da tailing module
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 93 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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