nebnext ultra ii directional rna library prep with sample purification beads  (New England Biolabs)


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    Name:
    NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads
    Description:
    NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads 96 rxns
    Catalog Number:
    e7765l
    Price:
    3525
    Size:
    96 rxns
    Category:
    mRNA Template Preparation for PCR
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    Structured Review

    New England Biolabs nebnext ultra ii directional rna library prep with sample purification beads
    NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads
    NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads 96 rxns
    https://www.bioz.com/result/nebnext ultra ii directional rna library prep with sample purification beads/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii directional rna library prep with sample purification beads - by Bioz Stars, 2020-04
    99/100 stars

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    rnaseq libraries

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    Related Articles

    Amplification:

    Article Title: Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
    Article Snippet: Briefly, RNASeq libraries were prepared using 200 ng of total RNA and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions, with mRNA enriched via poly-A-selection using oligoDT beads. .. The RNA was then thermally fragmented and converted to cDNA, adenylated for adaptor ligation and PCR amplified.

    RNA Sequencing Assay:

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: .. RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA). ..

    Ligation:

    Article Title: Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
    Article Snippet: Briefly, RNASeq libraries were prepared using 200 ng of total RNA and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions, with mRNA enriched via poly-A-selection using oligoDT beads. .. The RNA was then thermally fragmented and converted to cDNA, adenylated for adaptor ligation and PCR amplified.

    Generated:

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: .. RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA). ..

    Purification:

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: To reverse crosslinks, the combined eluates and RNA inputs were mixed with 312.5 μl NLS Elution Buffer (20 mM Tris-HCl pH 7.5, 10 mM EDTA, 2% N -lauroylsarcosine, 2.5 mM TCEP), 50 μl 5 M NaCl, and 12.5 μl Proteinase K (Thermo Fischer Scientific, cat# EO0491) and incubated at 60 °C for 2 h. RNAs were then purified by mixing them with 40 μl of Dynabeads MyOne Silane beads (Thermo Fischer Scientific, cat# 37002D) pre-rinsed in RLT buffer (QIAGEN, cat# 79216) and resuspended in 50 μl 5 M NaCl. .. RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA).

    Sequencing:

    Article Title: Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
    Article Snippet: RNAseq Twenty-four brain samples (8 biological replicates per treatment; control, 0.75 mM ACR, and 0.3 mM NAC + 0.75 mM ACR treatments) were sent to the Vanderbilt Technologies for Advanced Genomics (Vanderbilt University, Nashville, TN) for library preparation and sequencing on the Illumina Novaseq platform. .. Briefly, RNASeq libraries were prepared using 200 ng of total RNA and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions, with mRNA enriched via poly-A-selection using oligoDT beads.

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA). .. Paired-end sequencing was performed using a HiSeq4000 75 bp platform (Illumina, HiSeq 3000/4000 PE Cluster Kit and 150 cycle SBS Kit).

    Incubation:

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: To reverse crosslinks, the combined eluates and RNA inputs were mixed with 312.5 μl NLS Elution Buffer (20 mM Tris-HCl pH 7.5, 10 mM EDTA, 2% N -lauroylsarcosine, 2.5 mM TCEP), 50 μl 5 M NaCl, and 12.5 μl Proteinase K (Thermo Fischer Scientific, cat# EO0491) and incubated at 60 °C for 2 h. RNAs were then purified by mixing them with 40 μl of Dynabeads MyOne Silane beads (Thermo Fischer Scientific, cat# 37002D) pre-rinsed in RLT buffer (QIAGEN, cat# 79216) and resuspended in 50 μl 5 M NaCl. .. RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA).

    Formalin-fixed Paraffin-Embedded:

    Article Title: A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells
    Article Snippet: .. RNA-Seq libraries were generated using NEBNext® Ultra8482 II Directional RNA Library Preparation kit (New England Biolabs, cat# E7765S; following the protocol for rRNA Depleted FFPE/Strongly fragmented RNA). ..

    Expressing:

    Article Title: Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
    Article Snippet: Briefly, RNASeq libraries were prepared using 200 ng of total RNA and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions, with mRNA enriched via poly-A-selection using oligoDT beads. .. The sequence reads of the 24 samples were aligned to the Zebrafish transcriptome GRCz11 (NCBI) using Salmon v 0.12.0 producing an estimated transcript expression.

    Polymerase Chain Reaction:

    Article Title: Therapeutic potential of N-acetylcysteine in acrylamide acute neurotoxicity in adult zebrafish
    Article Snippet: Briefly, RNASeq libraries were prepared using 200 ng of total RNA and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer’s instructions, with mRNA enriched via poly-A-selection using oligoDT beads. .. The RNA was then thermally fragmented and converted to cDNA, adenylated for adaptor ligation and PCR amplified.

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  • 99
    New England Biolabs nebnext ultra ii directional rna library prep with sample purification beads
    Nebnext Ultra Ii Directional Rna Library Prep With Sample Purification Beads, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii directional rna library prep with sample purification beads/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii directional rna library prep with sample purification beads - by Bioz Stars, 2020-04
    99/100 stars
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