nebnext ultra end repair da tailing module  (New England Biolabs)


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  • 99
    Name:
    NEBNext Ultra End Repair dA Tailing Module
    Description:
    NEBNext Ultra End Repair dA Tailing Module 96 rxns
    Catalog Number:
    e7442l
    Price:
    746
    Size:
    96 rxns
    Category:
    DNA Template Preparation for PCR
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    Structured Review

    New England Biolabs nebnext ultra end repair da tailing module
    NEBNext Ultra End Repair dA Tailing Module
    NEBNext Ultra End Repair dA Tailing Module 96 rxns
    https://www.bioz.com/result/nebnext ultra end repair da tailing module/product/New England Biolabs
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra end repair da tailing module - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Predictable Molecular Adaptation of Coevolving Enterococcus faecium and Lytic Phage EfV12-phi1
    Article Snippet: .. The repaired DNA was dA-tailed using NEBNext Ultra End Repair (New England Biolabs) and sequence adapters were ligated using Blunt TA ligase master mix (New England Biolabs). ..

    Sonication:

    Article Title: Endogenous Parkin Preserves Dopaminergic Substantia Nigral Neurons following Mitochondrial DNA Mutagenic Stress
    Article Snippet: .. Briefly, 500 ng of total DNA was sonicated in 60 μl of nuclease-free ddH2 O and subjected to end-repair and 3′-dA-tailing using the NEBNext Ultra End-Repair/dA-Tailing Module (New England Biolabs) according to the vendor's instructions. .. A 20:1 molar excess of Duplex Sequencing adapters was ligated to the sample DNA using the NEBNext Ultra Ligation Module (New England Biolabs).

    Incubation:

    Article Title: Enteric infection induces Lark-mediated intron retention at the 5′ end of Drosophila genes
    Article Snippet: .. We added 3 μl of NEBNext Ultra End Repair/dA-Tailing Module Enzyme mix and 6.5 μl of Reaction buffer and incubated each tube at 20 °C for 30 min, then 65 °C for 30 min. .. The product of the reaction was purified using the Qiagen MinElute PCR Purification Kit; elution was made in 12.5 μl of Elution Buffer.

    other:

    Article Title: Utilization of Hybrid Assembly Approach to Determine the Genome of an Opportunistic Pathogenic Fungus, Candida albicans TIMM 1768
    Article Snippet: Briefly, 1 µg genomic DNA was end-prepped using the NEBNext End repair/dA-tailing Module (New England Biolabs, Inc., Ipswich, MA, USA) and the DNA was cleaned up using Agencourt AMPure XP (Beckman Coulter Inc., Brea, CA, USA).

    Article Title: Ultrasensitive and high-efficiency screen of de novo low-frequency mutations by o2n-seq
    Article Snippet: Mix the following components in a sterile nuclease-free tube: End Prep Enzyme Mix (NEBNext Ultra End Repair/dA-Tailing Module, NEB, catalogue number: E7442S) 3.0 μl, end repair reaction buffer (10 × ) 6.5 μl and fragmented DNA 55.5 μl, and place in a thermocycler, with the heated lid on.

    Article Title: Transcriptome-wide survey of pseudorabies virus using next- and third-generation sequencing platforms
    Article Snippet: NEBNext End repair / dA-tailing Module (New England Biolabs) was used for end repair, while NEB Blunt/TA Ligase Master Mix (New England Biolabs) was applied for adapter ligations.

    Ligation:

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: .. Klenow fragment polymerase (KF, 3′ → 5′ exo-), Nt.BsmAI NEase, T4 Phage β -glucosyltransferase (T4-β GT), UDP-glucose, Cutsmart buffer (20 mM Tris-acetate, 50 mM potassium acetate, 10 mM magnesium acetate and 0.1 mg ml–1 BSA, pH 7.9), NEB buffer 2 (10 mM Tris–HCl, 10 mM MgCl2 , 50 mM NaCl and 1 mM dithiothreitol, pH 7.9), NEB buffer 4 (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate and 1 mM dithiothreitol, pH 7.9), the NEBNext Ultra End Repair/dA-Tailing Module, the NEBNext Ultra Ligation Module and NEBNext Singleplex Oligos for Illumina were purchased from New England Biolabs (USA). .. Deoxynucleotide triphosphates (dNTPs), UNIQ-10 Spin Column Oligo DNA Purification Kits, EZ-10 Column DNA Purification Kits and 1 × phosphate buffer saline (PBS, pH 7.4) were ordered from Shanghai Sangon Biological Engineering Technology Services Co., Ltd. (Shanghai, China).

    Article Title: Multiple Long-Read Sequencing Survey of Herpes Simplex Virus Dynamic Transcriptome
    Article Snippet: .. The NEBNext End repair/dA-tailing Module (New England Biolabs) was used for the end repair, whereas the NEB Blunt/TA Ligase Master Mix (New England Biolabs) was utilized for the adapter ligation. .. The enzymatic steps (e.g.: RT, PCR, and ligation) were carried out in a Veriti Cycler (Applied Biosystems) according to the 1D protocol ( ; ).

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  • 99
    New England Biolabs nebnext ultra ii end repair da tailing module
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-09
    99/100 stars
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