nebnext da tailing modules  (New England Biolabs)


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  • 99
    Name:
    NEBNext dA Tailing Module
    Description:
    NEBNext dA Tailing Module 100 rxns
    Catalog Number:
    e6053l
    Price:
    416
    Size:
    100 rxns
    Category:
    DNA Template Preparation for PCR
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    Structured Review

    New England Biolabs nebnext da tailing modules
    NEBNext dA Tailing Module
    NEBNext dA Tailing Module 100 rxns
    https://www.bioz.com/result/nebnext da tailing modules/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nebnext da tailing modules - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Isolation:

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries
    Article Snippet: .. Using the RNA samples isolated from a human osteosarcoma line and rRNA-depleted with RTR2D protocol or the NEBNext protocol, we found that the overall percentages of different transcript categories were similar for both rRNA-depleted samples prepared with the RTR2D and NEBNext protocols ( A, a vs. b ). .. Scatter plot comparison analysis indicated that the expression levels of mRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were highly correlative in replicate experiments (R = 0.94) ( B, a & c) .

    Introduce:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Modification:

    Article Title: Comprehensive multi-center assessment of accuracy, reproducibility and bias of small RNA-seq methods for quantitative miRNA profiling
    Article Snippet: .. In addition, of the six labs that generated libraries using the NEBNext protocol, two of the labs used somewhat modified conditions based on options provided by the manufacturer and were excluded from the analysis ( Online Methods ). ..

    Generated:

    Article Title: Comprehensive multi-center assessment of accuracy, reproducibility and bias of small RNA-seq methods for quantitative miRNA profiling
    Article Snippet: .. In addition, of the six labs that generated libraries using the NEBNext protocol, two of the labs used somewhat modified conditions based on options provided by the manufacturer and were excluded from the analysis ( Online Methods ). ..

    Selection:

    Article Title: Nuclease-mediated depletion biases in ribosome footprint profiling libraries
    Article Snippet: .. The libraries depleted by RiboCop and legacy Ribo-Zero have indistinguishable size distributions; importantly, however, the RNaseH-mediated depletion by the NEBNext protocol resulted in a broad distribution of fragment sizes spanning nearly the entire range of the 15 to 40 nt size selection. ..

    Expressing:

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries
    Article Snippet: .. Scatter plot comparison analysis indicated that the expression levels of mRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were highly correlative in replicate experiments (R = 0.94) ( B, a & c) . .. Similarly, the expression levels of lncRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were also correlative in replicate experiments (R = 0.86 and 0.85) ( C, a & c) , although the correlation coefficients are slightly lower than that of the mRNA transcripts.

    Polymerase Chain Reaction:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

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  • 92
    New England Biolabs nebnext da tailing module
    Nebnext Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext da tailing module/product/New England Biolabs
    Average 92 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    nebnext da tailing module - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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