nebnext da tailing module  (New England Biolabs)


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    Name:
    NEBNext dA Tailing Module
    Description:
    NEBNext dA Tailing Module 100 rxns
    Catalog Number:
    e6053l
    Price:
    416
    Size:
    100 rxns
    Category:
    DNA Template Preparation for PCR
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    Structured Review

    New England Biolabs nebnext da tailing module
    NEBNext dA Tailing Module
    NEBNext dA Tailing Module 100 rxns
    https://www.bioz.com/result/nebnext da tailing module/product/New England Biolabs
    Average 99 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    nebnext da tailing module - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    RNA Sequencing Assay:

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries
    Article Snippet: .. We further compared the transcriptomic landscape in response to MDM2 inhibitor Nutlin3A in human osteosarcoma line SJSA1 cells based on the RNA-seq analysis of the libraries prepared with the RTR2D protocol vs. the NEBNext protocol. .. Upon Nutlin3A treatment, we found that, while the NEBNext protocol yielded 295 up-regulated and 343 down-regulated mRNA transcripts ( A, a ), the RTR2D protocol produced 539 up-regulated and 646 down-regulated mRNA transcripts ( A, b ), suggesting that the RTR2D-based rRNA depletion process may preserve mRNA and lncRNA transcripts more effectively and thus lead to the identification of more differentially expressed transcripts.

    Isolation:

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries
    Article Snippet: .. Using the RNA samples isolated from a human osteosarcoma line and rRNA-depleted with RTR2D protocol or the NEBNext protocol, we found that the overall percentages of different transcript categories were similar for both rRNA-depleted samples prepared with the RTR2D and NEBNext protocols ( A, a vs. b ). .. Scatter plot comparison analysis indicated that the expression levels of mRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were highly correlative in replicate experiments (R = 0.94) ( B, a & c) .

    Introduce:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Modification:

    Article Title: Comprehensive multi-center assessment of accuracy, reproducibility and bias of small RNA-seq methods for quantitative miRNA profiling
    Article Snippet: .. In addition, of the six labs that generated libraries using the NEBNext protocol, two of the labs used somewhat modified conditions based on options provided by the manufacturer and were excluded from the analysis ( Online Methods ). ..

    Generated:

    Article Title: Comprehensive multi-center assessment of accuracy, reproducibility and bias of small RNA-seq methods for quantitative miRNA profiling
    Article Snippet: .. In addition, of the six labs that generated libraries using the NEBNext protocol, two of the labs used somewhat modified conditions based on options provided by the manufacturer and were excluded from the analysis ( Online Methods ). ..

    Expressing:

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries
    Article Snippet: .. Scatter plot comparison analysis indicated that the expression levels of mRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were highly correlative in replicate experiments (R = 0.94) ( B, a & c) . .. Similarly, the expression levels of lncRNA transcripts prepared with the RTR2D protocol and the NEBNext protocol were also correlative in replicate experiments (R = 0.86 and 0.85) ( C, a & c) , although the correlation coefficients are slightly lower than that of the mRNA transcripts.

    Polymerase Chain Reaction:

    Article Title: RNA sequencing by direct tagmentation of RNA/DNA hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

    Article Title: RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids
    Article Snippet: .. Besides, the NEBNext protocol used short RNA fragments for reverse transcription and fewer cycles of PCR amplification, which should introduce less GC bias than the other protocols. .. We then compared the GC distribution of genes detected by scSHERRY or Smart-seq2 with NEBNext results. scSHERRY, which is free from second-strand synthesis and preamplification, produced a distribution similar to the standard.

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  • 99
    New England Biolabs nebnext ultra ii end repair da tailing module
    Nebnext Ultra Ii End Repair Da Tailing Module, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext ultra ii end repair da tailing module/product/New England Biolabs
    Average 99 stars, based on 67 article reviews
    Price from $9.99 to $1999.99
    nebnext ultra ii end repair da tailing module - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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