neb3 buffer (New England Biolabs)

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Name:

NEBuffer 3

Description:

NEBuffer 3 5 0 ml

Catalog Number:

b7003s

Price:

Size:

5 0 ml

Category:

Buffers

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Structured Review

New England Biolabs neb3 buffer

NEBuffer 3 5 0 ml
https://www.bioz.com/result/neb3 buffer/product/New England Biolabs
Average 98 stars, based on 5 article reviews
Price from $9.99 to $1999.99

neb3 buffer - by Bioz Stars, 2021-01

98/100 stars

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Images

Protease Inhibitor:

Article Title: Host-regulated Hepatitis B Virus Capsid Assembly in a Mammalian Cell-free System
Article Snippet: .. Pipette tips (Denville Scientific, catalog numbers: P1096-FR, P1121, P1122, P1126) 1.5 ml microcentrifuge tube (Denville Scientific, catalog number: C2170) Gloves and lab coat (Denville Scientific, catalog number: G4162; Medline Industries, catalog number: 83044QHW) Proteinase K, RNA grade (Thermo Fisher Scientific, Invitrogen™, catalog number: 25530049) Sodium dodecyl sulfate (SDS) (Sigma-Aldrich, catalog number: L4509-1KG) Phenol solution (Sigma-Aldrich, catalog number: P4557) Chloroform (Fisher Scientific, catalog number: BP1145-1) Sodium acetate (Sigma-Aldrich, catalog number: S2889-1KG) Ethyl alcohol (EtOH) (AmericanBio, catalog number: AB00515-00100) UltraPure™ DNase/RNase-Free Distilled Water (Thermo Fisher Scientific, Invitrogen™, catalog number: 10977015) Agarose (Thermo Fisher Scientific, Invitrogen™, catalog number: 15510027) TNT® Coupled Rabbit Reticulocyte Lysate (RRL) (Promega, catalog number: L4610) EasyTag™ L-[35 S]-Methionine (PerkinElmer, catalog number: NEG709A001MC) RNasin® Plus Ribonuclease Inhibitor (Promega, catalog number: N2611) RNaseZap™ RNase Decontamination Solution (Thermo Fisher Scientific, Invitrogen™, catalog number: AM9780) RNase A, DNase and protease-free (Thermo Fisher Scientific, Thermo Scientific™, catalog number: EN0531) NEBuffer 3 (New England Biolabs, catalog number: B7003S) Alkaline Phosphatase, Calf Intestinal (CIAP) (New England Biolabs, catalog number: M0290S) Tris Base (Fisher Scientific, catalog number: BP152-10) Ethylenediaminetetraacetic acid, EDTA (Sigma-Aldrich, catalog number: E5134) Sodium fluoride (Sigma-Aldrich, catalog number: S7920) Sodium pyrophosphate tetrabasic decahydrate (Sigma-Aldrich, catalog number: S6422) β-Glycerophosphate (Sigma-Aldrich, catalog number: G6251) Sodium orthovanadate (Sigma-Aldrich, catalog number: S6508) cOmplete™, EDTA-free Protease Inhibitor Cocktail (Roche Diagnostics, catalog number: 04693132001) TE buffer (see Recipes) 10× phosphatase inhibitors (PPI) (see Recipes) 25× protease inhibitor (see Recipes) .. Pipettes (Gilson, P1000, P200, P20, P2) Fume hood ( e.g. , Protector Xstream Laboratory Hood, Labconco) 30 °C/37 °C Oven (SciGene, Robbins Scientific, model: Model 400) Microcentrifuge (Fisher Scientific, Fisherbrand™, model: accuSpin™ Micro 17) Spectrophotometer (Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™1000)

Restriction Assay:

Article Title: Transcription Blockage by Bulky End-Termini at Single-Strand Breaks in the DNA Template: Differential Effects of 5′ and 3′ Adducts
Article Snippet: .. The restriction assay was carried out in a total volume of 50 μL containing 0.83 pmol of radiolabeled annealed insert, 1X NEBuffer 3 (NEB) and 1X BSA (NEB) with or without 20 U of SalI. .. EDTA was added to a concentration of 20 mM and the restriction enzyme was inactivated by heating at 65°C for 20min.

Transferring:

Incubation:

Article Title: Genetic relationships between Candida albicans strains isolated from dental plaque, trachea, and bronchoalveolar lavage fluid from mechanically ventilated intensive care unit patients
Article Snippet: .. For Bss HII digestion, the plug was transferred to a microcentrifuge tube containing 500 µl of buffer 3 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2 , 1 mM dithiothreitol), and incubated on ice for 30 min. After aspirating buffer, the plug was placed in 160 µl of buffer 3 containing 4 U of Bss HII (New England BioLabs), and incubated overnight at 50°C. ..

Article Title: In Vitro Studies of DNA Mismatch Repair Proteins
Article Snippet: .. For the construction of mismatched substrates, 400 μg of pSCW01 or pSCW02 was incubated with 300 U of Nt.BstNBI endonuclease (New England BioLabs) at 55°C for 2 hr in 1.0 ml final volume in 1 × Buffer 3 (New England BioLabs) followed by the addition of another 300 U of Nt.BstNBI endonuclease and further incubation at 55°C for 2 hr. ..