ndei bamhi restriction sites  (New England Biolabs)


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  • 99
    Name:
    NdeI
    Description:
    NdeI 20 000 units
    Catalog Number:
    r0111l
    Price:
    269
    Size:
    20 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ndei bamhi restriction sites
    NdeI
    NdeI 20 000 units
    https://www.bioz.com/result/ndei bamhi restriction sites/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ndei bamhi restriction sites - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Ligation:

    Article Title: Comparative analysis of the end-joining activity of several DNA ligases
    Article Snippet: .. General materials T4 DNA ligase reaction buffer (50 mM Tris-HCl pH 7.5 @ 25°C, 1 mM ATP and 10 mM MgCl2 ) as a 10x stock, NEBNext® Quick Ligation reaction buffer (66 mM Tris pH 7.6 @ 25°C, 10 mM MgCl2 , 1 mM DTT, 1 mM ATP, 6% Polyethylene glycol (PEG 6000)) as a 5x stock, restriction endonucleases EcoRV, NruI, BstNI, Hpy188I, NdeI, BamHI, as well as λ DNA, 1 M DTT, 6x Purple Loading Dye with SDS, Proteinase K and 10 mM ATP were obtained from New England Biolabs (NEB, Ipswich, MA). .. Tris-HCl (1 M pH 7.5 @ 25°C) was obtained from Amresco (Solon, OH).

    Random Primed:

    Article Title: Enhanced transgene expression in rice following selection controlled by weak promoters
    Article Snippet: .. About 3 μg genomic DNA per sample was digested by Nde I (NEB, Beverly, MA) and hybridized with a HPT probe which was prepared using a random primed digoxigenin-DNA labeling kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Diagnostics, Basel, Switzerland) according to the manufacturer’s protocol. .. Primers for probe amplification are listed in Additional file : Table S1.

    Labeling:

    Article Title: Enhanced transgene expression in rice following selection controlled by weak promoters
    Article Snippet: .. About 3 μg genomic DNA per sample was digested by Nde I (NEB, Beverly, MA) and hybridized with a HPT probe which was prepared using a random primed digoxigenin-DNA labeling kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Diagnostics, Basel, Switzerland) according to the manufacturer’s protocol. .. Primers for probe amplification are listed in Additional file : Table S1.

    Generated:

    Article Title: DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI
    Article Snippet: .. Linear DNA substrates were generated by incubating 4 nM plasmid DNA with 1 U/μl of AlwNI or 2 U/μl of NdeI in NEBuffer 2 (New England Biolabs, MA, USA). .. The DNA was purified by phenol/chloroform and chloroform extraction followed by ethanol precipitation.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Control of alternative splicing by forskolin through hnRNP K during neuronal differentiation
    Article Snippet: .. Some cultures were pretreated with 10 µM of H89 (N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamidedihydrochloride, Sigma, #B1427) for 10 min. To differentiate products of Snap25 endogenous mRNA transcripts from exons 5a and 5b, we digested 1.0 µl of 32 P-labelled-PCR products with AvaII and NdeI restriction enzymes in buffer 4 (New England Biolabs) in a 10 -µl reaction at 37°C for 1 h and run in 6% denaturing polyacrylamide gel electrophoresis (PAGE) gel. .. For minigene splicing reporters, 24 cycles of PCR were carried out and products resolved in 3% agarose gels stained with ethidium bromide.

    DNA Labeling:

    Article Title: Enhanced transgene expression in rice following selection controlled by weak promoters
    Article Snippet: .. About 3 μg genomic DNA per sample was digested by Nde I (NEB, Beverly, MA) and hybridized with a HPT probe which was prepared using a random primed digoxigenin-DNA labeling kit (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche Diagnostics, Basel, Switzerland) according to the manufacturer’s protocol. .. Primers for probe amplification are listed in Additional file : Table S1.

    Plasmid Preparation:

    Article Title: DNA cleavage and methylation specificity of the single polypeptide restriction-modification enzyme LlaGI
    Article Snippet: .. Linear DNA substrates were generated by incubating 4 nM plasmid DNA with 1 U/μl of AlwNI or 2 U/μl of NdeI in NEBuffer 2 (New England Biolabs, MA, USA). .. The DNA was purified by phenol/chloroform and chloroform extraction followed by ethanol precipitation.

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  • 99
    New England Biolabs ndei bamhi restriction sites
    Ndei Bamhi Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei bamhi restriction sites/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ndei bamhi restriction sites - by Bioz Stars, 2020-07
    99/100 stars
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