prsfduet 1  (Millipore)


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    Structured Review

    Millipore prsfduet 1
    In vivo citrate detection using CF98 in E. coli expressing the gene encoding citrate carrier CitT. A, Time courses of the fluorescence intensities of CF98 from E. coli BL21(DE3)/pECF98+pRCITT and E. coli <t>BL21(DE3)/pECF98+pRSFDuet-1.</t> The fluorescence intensities of CF98 from aliquots of the cell suspension were measured in 50 mM Na 2 HPO 4 -NaH 2 PO 4 buffer (pH 7.0). At 50 s after starting the measurements, the citrate-containing buffer was added to change the extracellular citrate concentration to the indicated citrate concentrations in the cuvette. The fluorescence intensities (excitation at 504 nm, emission at 525 nm) at each time point in three independent experiments were averaged. B, Excitation spectrum (emission at 525 nm) of CF98 from aliquots of the cell suspension of E. coli BL21(DE3)/pECF98+pRCITT was measured 150 s after the addition of the citrate-containing buffer to change the citrate concentration from 0 to 2.5 mM. The control excitation spectrum was measured without addition of citrate.
    Prsfduet 1, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1482 article reviews
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    prsfduet 1 - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "Generation of Circularly Permuted Fluorescent-Protein-Based Indicators for In Vitro and In Vivo Detection of Citrate"

    Article Title: Generation of Circularly Permuted Fluorescent-Protein-Based Indicators for In Vitro and In Vivo Detection of Citrate

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064597

    In vivo citrate detection using CF98 in E. coli expressing the gene encoding citrate carrier CitT. A, Time courses of the fluorescence intensities of CF98 from E. coli BL21(DE3)/pECF98+pRCITT and E. coli BL21(DE3)/pECF98+pRSFDuet-1. The fluorescence intensities of CF98 from aliquots of the cell suspension were measured in 50 mM Na 2 HPO 4 -NaH 2 PO 4 buffer (pH 7.0). At 50 s after starting the measurements, the citrate-containing buffer was added to change the extracellular citrate concentration to the indicated citrate concentrations in the cuvette. The fluorescence intensities (excitation at 504 nm, emission at 525 nm) at each time point in three independent experiments were averaged. B, Excitation spectrum (emission at 525 nm) of CF98 from aliquots of the cell suspension of E. coli BL21(DE3)/pECF98+pRCITT was measured 150 s after the addition of the citrate-containing buffer to change the citrate concentration from 0 to 2.5 mM. The control excitation spectrum was measured without addition of citrate.
    Figure Legend Snippet: In vivo citrate detection using CF98 in E. coli expressing the gene encoding citrate carrier CitT. A, Time courses of the fluorescence intensities of CF98 from E. coli BL21(DE3)/pECF98+pRCITT and E. coli BL21(DE3)/pECF98+pRSFDuet-1. The fluorescence intensities of CF98 from aliquots of the cell suspension were measured in 50 mM Na 2 HPO 4 -NaH 2 PO 4 buffer (pH 7.0). At 50 s after starting the measurements, the citrate-containing buffer was added to change the extracellular citrate concentration to the indicated citrate concentrations in the cuvette. The fluorescence intensities (excitation at 504 nm, emission at 525 nm) at each time point in three independent experiments were averaged. B, Excitation spectrum (emission at 525 nm) of CF98 from aliquots of the cell suspension of E. coli BL21(DE3)/pECF98+pRCITT was measured 150 s after the addition of the citrate-containing buffer to change the citrate concentration from 0 to 2.5 mM. The control excitation spectrum was measured without addition of citrate.

    Techniques Used: In Vivo, Expressing, Fluorescence, Concentration Assay

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