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PEQLAB nd 1000
Nd 1000, supplied by PEQLAB, used in various techniques. Bioz Stars score: 95/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nd 1000/product/PEQLAB
Average 95 stars, based on 41 article reviews
Price from $9.99 to $1999.99
nd 1000 - by Bioz Stars, 2019-12
95/100 stars

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Related Articles

Clone Assay:

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. The expression levels of target (gh , igf-1 , igf-2a , igf-2b , hsp70 , hsp90 ) and reference (ef1a , 18s , 40s ) genes were determined by quantitative real-time PCR (qRT-PCR), using specific primers.

Amplification:

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany). .. We further added 10 µg BSA per reaction to reduce the inhibitory effect of melanin on reverse transcriptase and DNA polymerase efficiencies . qRT-PCR was performed using SybrGreen premix (Invitrogen, Carlsbad, CA, USA) in 30 µl final reactions and an iCycler iQ Real Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

Mass Spectrometry:

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: Those larvae were not observed under the microscope (to avoid any influence on gene expression) but were immediately euthanized using MS-222, rinsed with deionized water, preserved in a RNA Stabilization Reagent and kept at -20°C following the procedure suggested by the supplier (Qiagen, Hilden, Germany). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: Total RNA was isolated from 50 to 75 mg of 14-day-old seedlings (cultured under long-day conditions on MS + 1% sucrose plates) with the RNeasy plant mini kit (Qiagen, Germany). .. RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

Stable Transfection:

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany). .. The PCR program consisted of an initializing step (95 °C for 3 min) and 40 amplification cycles comprising 95 °C for 45 s, 56 °C for 25 s, and 72 °C for 30 s. Each sample/primer combination was measured in triplicate and the Ct values were averaged before normalisation.

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Synthesized:

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany). .. Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany).

Quantitative RT-PCR:

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Paragraph title: Isolation of RNA and qRT-PCR ... Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany).

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. The ef1a , 18s , 40s genes were chosen as housekeeping genes since qBase+ software revealed that these mRNA levels were stable throughout analyzed samples (M < 0.4); M gives the gene stability and M < 0.5 is typical for stably expressed reference genes [ ].

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: Paragraph title: RNA isolation and quantitative real-time RT-PCR (qPCR) ... RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

SYBR Green Assay:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions. .. RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions.

Article Title: 3,5-Diiodo-L-Thyronine (3,5-T2) Exerts Thyromimetic Effects on Hypothalamus-Pituitary-Thyroid Axis, Body Composition, and Energy Metabolism in Male Diet-Induced Obese Mice
Article Snippet: Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab). .. Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab).

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany). .. Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany).

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively. .. RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

Incubation:

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively. .. GAPDH expression was examined as an internal control [ ].

Article Title: The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria
Article Snippet: Each disc was placed in 750 µL Trifast® FL (PEQLAB, Germany), homogenized, and incubated for 5 min at RT. .. RNA purity and concentration were determined by NanoDrop® ND–1000 (Peqlab Biotechnologie GmbH, Erlangen, Germany).

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: After obtaining the aqueous phase by incubation in 160 μl chloroform, RNA was extracted using the InviTrap® Spin tissue RNA MiniKit (STRATEC Biomedical AG, Berlin-Buch, Germany) following the manufacturer’s instructions. .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Article Title: Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Article Snippet: RNA was eluted in 100 μl nuclease free water and the concentration was determined using a Nanodrop ND 1000 (PeqLab, Germany). .. RNA was eluted in 100 μl nuclease free water and the concentration was determined using a Nanodrop ND 1000 (PeqLab, Germany).

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent). .. RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent).

Formalin-fixed Paraffin-Embedded:

Article Title: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma
Article Snippet: To ensure high tumour DNA content, FFPE tissue sections were stained with H & E and histologically examined by an expert neuropathologist Sections showing a tumour cell content of more than 80 % were directly subjected to DNA extraction.. .. Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany).

Solubility:

Article Title: Interaction of two antitumor peptides with membrane lipids – Influence of phosphatidylserine and cholesterol on specificity for melanoma cells
Article Snippet: Stock solutions of R-DIM-P-LF11-322 and DIM-LF11-318 were prepared in acetic acid (0.1% v/v) at a concentration of about 3 mg/ml and treated with ultrasonication for better solubility. .. The peptide concentrations of the non-labeled peptides were determined via measurement of UV-absorbance of tryptophan at 280 nm (using NanoDrop ND 1000 (Peqlab, VWR International, Inc. Erlangen, Germany)).

Expressing:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions. .. Relative mRNA levels were determined by semiquantitative RT-PCR (qRT-PCR) using the TaqMan Gene Expression Assay Technique and the 7900HT Fast Real-Time PCR System (Applied Biosystems).

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively. .. The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively.

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: Those larvae were not observed under the microscope (to avoid any influence on gene expression) but were immediately euthanized using MS-222, rinsed with deionized water, preserved in a RNA Stabilization Reagent and kept at -20°C following the procedure suggested by the supplier (Qiagen, Hilden, Germany). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Article Title: 3,5-Diiodo-L-Thyronine (3,5-T2) Exerts Thyromimetic Effects on Hypothalamus-Pituitary-Thyroid Axis, Body Composition, and Energy Metabolism in Male Diet-Induced Obese Mice
Article Snippet: Paragraph title: Quantitative real-time PCR of TH-responsive gene expression in the liver and pituitary ... Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab).

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany). .. The cDNA was synthesized by reverse transcription using TaqMan® Reverse Transcription Reagents (Applied Biosystems) for RNA concentrations > 50 ng/µL or Sensiscript® Reverse Transcription Kit (QIAGEN GmbH, Hilden, Germany) for RNA concentrations ≤50 ng/µL. cDNA was stored at −20 °C until further processing. qPCR was carried out using the LightCycler® Fast Start DNA Master SYBR® Green I Kit (ROCHE Diagnostics-Applied Science, Mannheim, Germany) according to the following cycle program: Initial denaturation was for 10 min at 95 °C followed by 50 cycles with 5 s at 98 °C, 7 s at 64 °C, and 9 s at 72 °C.

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: Paragraph title: RNA isolation and genome-wide expression profiling ... RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent).

Genome Wide:

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: Paragraph title: RNA isolation and genome-wide expression profiling ... RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent).

Article Title: Germline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family
Article Snippet: Concentration of extracted DNA was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. DNA concentration was adjusted to 50–80 ng/μl.

Cell Culture:

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: Total RNA was isolated from 50 to 75 mg of 14-day-old seedlings (cultured under long-day conditions on MS + 1% sucrose plates) with the RNeasy plant mini kit (Qiagen, Germany). .. RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

Generated:

Article Title: A Genome-Wide Association Study Identifies Risk Loci to Equine Recurrent Uveitis in German Warmblood Horses
Article Snippet: DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany).

Polymerase Chain Reaction:

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively. .. GAPDH expression was examined as an internal control [ ].

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany). .. We further added 10 µg BSA per reaction to reduce the inhibitory effect of melanin on reverse transcriptase and DNA polymerase efficiencies . qRT-PCR was performed using SybrGreen premix (Invitrogen, Carlsbad, CA, USA) in 30 µl final reactions and an iCycler iQ Real Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

Article Title: Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing
Article Snippet: Paragraph title: PCR/purification ... Quantity of purified amplicons was determined using ND-1000 (Peqlab, Erlangen, Germany).

Article Title: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma
Article Snippet: Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany). .. Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany).

Injection:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: RNA extraction from mouse liver and real-time quantitative PCR Liver samples (100 mg) were homogenized in 1 ml TRIzol® (Invitrogen) in a TissueLyser (Qiagen) at 20 Hz for 6 min (n = 4 per group at t = 0 min and 120 min after pyruvate injection). .. RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions.

Binding Assay:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions. .. Relative mRNA levels were determined by semiquantitative RT-PCR (qRT-PCR) using the TaqMan Gene Expression Assay Technique and the 7900HT Fast Real-Time PCR System (Applied Biosystems).

DNA Extraction:

Article Title: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma
Article Snippet: To ensure high tumour DNA content, FFPE tissue sections were stained with H & E and histologically examined by an expert neuropathologist Sections showing a tumour cell content of more than 80 % were directly subjected to DNA extraction.. .. Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany).

Article Title: RASSF1A Site-Specific Methylation Hotspots in Cancer and Correlation with RASSF1C and MOAP-1
Article Snippet: Paragraph title: 4.3. DNA Isolation and Bisulfite Treatment ... Extraction of genomic DNA from biopsies and cells was performed using the QIAGEN Allprep DNA/RNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany).

Nucleic Acid Electrophoresis:

Article Title: Validation of Deleterious Mutations in Vorderwald Cattle
Article Snippet: Genomic DNA was isolated from EDTA-blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1% agarose gels (peqGold Universal Agarose, Peqlab Biotechnology). .. After quality control (genotyping rate per SNP and animal > 0.98), filtering for Hardy-Weinberg equilibrium (HWE) (p < 0.000001) using SAS/Genetics, version 9.4 (Statistical Analysis System, SAS-Institute, Cary, NE, USA), 44,811 SNPs were left for the analysis.

Article Title: Genome-wide association study for hereditary ataxia in the Parson Russell Terrier and DNA-testing for ataxia-associated mutations in the Parson and Jack Russell Terrier
Article Snippet: Genomic DNA was isolated using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1 % agarose gels (peqGold Universal Agarose, Peqlab Biotechnologie). .. We performed a GWAS in 44 PRT using the canine Illumina high density beadchip (Illumina, San Diego, CA, USA) containing 173,662 single nucleotide polymorphisms (SNPs).

Methylation:

Article Title: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma
Article Snippet: Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany). .. One micrograms of extracted DNA as well as CpGenome Universal Methylated DNA (Chemicon International, Temecula, CA) and CpGenome Universal Unmethylated DNA (Chemicon International) as controls were subjected to bisulfite treatment using the EpiTect Bisulfite Kit (Qiagen).

Isolation:

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Paragraph title: Isolation of RNA and qRT-PCR ... Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany).

Article Title: 3,5-Diiodo-L-Thyronine (3,5-T2) Exerts Thyromimetic Effects on Hypothalamus-Pituitary-Thyroid Axis, Body Composition, and Energy Metabolism in Male Diet-Induced Obese Mice
Article Snippet: Total mRNA from pituitaries was isolated using the Aurum kit (Bio-Rad) following manufacturer's guidelines. .. Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab).

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: To this end, the medium was removed and the cells were fixed with 4% paraformaldehyde (20 min) and stained with Alizarin S Red (Sigma Aldrich) (10 min). .. Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany). .. RNA was stored at −80 °C until further processing.

Article Title: Validation of Deleterious Mutations in Vorderwald Cattle
Article Snippet: Genomic DNA was isolated from EDTA-blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1% agarose gels (peqGold Universal Agarose, Peqlab Biotechnology).

Article Title: Genome-wide association study for hereditary ataxia in the Parson Russell Terrier and DNA-testing for ataxia-associated mutations in the Parson and Jack Russell Terrier
Article Snippet: Genomic DNA was isolated using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1 % agarose gels (peqGold Universal Agarose, Peqlab Biotechnologie).

Article Title: Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Article Snippet: Paragraph title: RNA isolation using the MoBio Powersoil Microbiome Total RNA Kit ... RNA was eluted in 100 μl nuclease free water and the concentration was determined using a Nanodrop ND 1000 (PeqLab, Germany).

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: Paragraph title: RNA isolation and quantitative real-time RT-PCR (qPCR) ... RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: Paragraph title: RNA isolation and genome-wide expression profiling ... RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent).

Article Title: A Genome-Wide Association Study Identifies Risk Loci to Equine Recurrent Uveitis in German Warmblood Horses
Article Snippet: For genotyping we isolated genomic DNA from EDTA blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany).

Labeling:

Article Title: Interaction of two antitumor peptides with membrane lipids – Influence of phosphatidylserine and cholesterol on specificity for melanoma cells
Article Snippet: The C-terminally amidated peptides R-DIM-P-LF11-322 ( PFWRIRIRR-P-RRIRIRWFP-NH2 , M = 2677.4 g/mol), DIM-LF11-318 ( FWQRRIRRWRR-FWQRRIRRWRR-NH2 , M = 3413.1 g/mol) and the fluorescently labeled peptide (5–6)- FAM-R-DIM-P-LF11-322 ((5–6)- FAM-PFWRIRIRR-P-RRIRIRWFP-NH 2, M = 3035.7 g/mol) were purchased from PolyPeptide Group (San Diego, CA, USA). .. The peptide concentrations of the non-labeled peptides were determined via measurement of UV-absorbance of tryptophan at 280 nm (using NanoDrop ND 1000 (Peqlab, VWR International, Inc. Erlangen, Germany)).

Purification:

Article Title: Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing
Article Snippet: PCR products were purified using Agencourt AMPure XP (Beckman Coulter GmbH, Krefeld, Germany). .. Quantity of purified amplicons was determined using ND-1000 (Peqlab, Erlangen, Germany). .. For some primer combinations, PCR amplification yielded an additional 2.5 – 3.0 kb PCR product (see and ).

Reverse Transcription Polymerase Chain Reaction:

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: Paragraph title: Quantitative reverse transcription (RT)-PCR ... The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively.

Microscopy:

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: Those larvae were not observed under the microscope (to avoid any influence on gene expression) but were immediately euthanized using MS-222, rinsed with deionized water, preserved in a RNA Stabilization Reagent and kept at -20°C following the procedure suggested by the supplier (Qiagen, Hilden, Germany). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Lysis:

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Eyecups were immersed in 350 µl lysis buffer (Buffer RLT, Qiagen) and homogenized with a TissueLyser II (Qiagen). .. Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany).

Article Title: Validation of Deleterious Mutations in Vorderwald Cattle
Article Snippet: Genomic DNA was isolated from EDTA-blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1% agarose gels (peqGold Universal Agarose, Peqlab Biotechnology).

Article Title: Genome-wide association study for hereditary ataxia in the Parson Russell Terrier and DNA-testing for ataxia-associated mutations in the Parson and Jack Russell Terrier
Article Snippet: Genomic DNA was isolated using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1 % agarose gels (peqGold Universal Agarose, Peqlab Biotechnologie).

Article Title: Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Article Snippet: The ice-cold resuspension of the pelleted cells in the MoBio lysis buffer was added to the supplied bead tubes filled with 500 μl ice-cold phenol-choloroform-isoamylalcohol (PCI) solution (Carl Roth, Germany). .. RNA was eluted in 100 μl nuclease free water and the concentration was determined using a Nanodrop ND 1000 (PeqLab, Germany).

Article Title: A Genome-Wide Association Study Identifies Risk Loci to Equine Recurrent Uveitis in German Warmblood Horses
Article Snippet: For genotyping we isolated genomic DNA from EDTA blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany).

Software:

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Article Title: Germline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family
Article Snippet: Concentration of extracted DNA was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. We performed a high-density genome-wide association analysis employing the Illumina bovine high density beadchip comprising 777,962 SNPs with a mean spacing of 3.4 kb across the entire bovine genome.

Article Title: A Genome-Wide Association Study Identifies Risk Loci to Equine Recurrent Uveitis in German Warmblood Horses
Article Snippet: DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany).

Real-time Polymerase Chain Reaction:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: Paragraph title: RNA extraction from mouse liver and real-time quantitative PCR ... RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions.

Article Title: Retinal S-opsin dominance in Ansell’s mole-rats (Fukomys anselli) is a consequence of naturally low serum thyroxine
Article Snippet: Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany). .. Total RNA was quantified by using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany).

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. The ef1a , 18s , 40s genes were chosen as housekeeping genes since qBase+ software revealed that these mRNA levels were stable throughout analyzed samples (M < 0.4); M gives the gene stability and M < 0.5 is typical for stably expressed reference genes [ ].

Article Title: 3,5-Diiodo-L-Thyronine (3,5-T2) Exerts Thyromimetic Effects on Hypothalamus-Pituitary-Thyroid Axis, Body Composition, and Energy Metabolism in Male Diet-Induced Obese Mice
Article Snippet: Paragraph title: Quantitative real-time PCR of TH-responsive gene expression in the liver and pituitary ... Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab).

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: Paragraph title: 4.7. RNA Isolation and Quantitative PCR (qPCR) of CTLA-4 Genes of MSCs ... Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany).

Article Title: The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity
Article Snippet: Paragraph title: RNA isolation and quantitative real-time RT-PCR (qPCR) ... RNA quantity and quality were assessed using the Nanodrop ND-1000 (Peqlab, Germany) and BioAnalyser 2100 (Agilent, USA) device, respectively.

RNA Extraction:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: Paragraph title: RNA extraction from mouse liver and real-time quantitative PCR ... RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions.

Article Title: The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria
Article Snippet: Paragraph title: RNA extraction from FTA® cards and reverse transcription ... RNA purity and concentration were determined by NanoDrop® ND–1000 (Peqlab Biotechnologie GmbH, Erlangen, Germany).

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: For total RNA extraction, the larval pool (~30 larvae) of each replicate was homogenized in 800 μl Tri-Reagent (Sigma-Aldrich, Missouri, USA). .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany).

Agarose Gel Electrophoresis:

Article Title: Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing
Article Snippet: Fragment sizes were inspected on a 0.7% TAE agarose gel. .. Quantity of purified amplicons was determined using ND-1000 (Peqlab, Erlangen, Germany).

Electrophoresis:

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: Total RNA was extracted from human cell lines using the TRIzol Reagent according to the manufacturer’s protocol. .. The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively. .. The cDNA templates were synthesized using Quanti-Tect Reverse Transcription Kit (Qiagen, Hilden, Germany).

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: Total RNA isolation and DNase I digestion was performed via RNeasy kits (Qiagen) according to the manufacturer’s instructions. .. RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent). .. Medicago GeneChip hybridizations were carried out as described previously [ ].

Random Hexamer Labeling:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: 200 μl chloroform was added, samples were vortexed and centrifuged for 15 min at 14,000 g. The supernatant was mixed with 100% ethanol (1/3 v/v) and RNA was extracted using the RNA extraction kit (NucleoSpin® RNA kit, Macherey-Nagel) according to the manufacturer’s instructions. .. RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions. .. Relative mRNA levels were determined by semiquantitative RT-PCR (qRT-PCR) using the TaqMan Gene Expression Assay Technique and the 7900HT Fast Real-Time PCR System (Applied Biosystems).

Spectrophotometry:

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: After obtaining the aqueous phase by incubation in 160 μl chloroform, RNA was extracted using the InviTrap® Spin tissue RNA MiniKit (STRATEC Biomedical AG, Berlin-Buch, Germany) following the manufacturer’s instructions. .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. From the resulting total RNA, 680 ng were transcribed using the Quanta qScript cDNA Synthesis Kit (Promega, Germany) according to the manufacturer’s instructions, including an additional gDNA wipe out step prior to transcription [Quanta PerfeCta DNase I Kit (Promega, Germany)].

Article Title: Pre-announcement of symbiotic guests: transcriptional reprogramming by mycorrhizal lipochitooligosaccharides shows a strict co-dependency on the GRAS transcription factors NSP1 and RAM1
Article Snippet: Total RNA isolation and DNase I digestion was performed via RNeasy kits (Qiagen) according to the manufacturer’s instructions. .. RNA preparations were quality-checked by spectrophotometry using a NanoDrop ND-1000 (Peqlab) and by capillary electrophoresis using a Bioanalyzer (Agilent). .. Medicago GeneChip hybridizations were carried out as described previously [ ].

Produced:

Article Title: Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing
Article Snippet: Quantity of purified amplicons was determined using ND-1000 (Peqlab, Erlangen, Germany). .. For some primer combinations, PCR amplification yielded an additional 2.5 – 3.0 kb PCR product (see and ).

Concentration Assay:

Article Title: Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet
Article Snippet: 200 μl chloroform was added, samples were vortexed and centrifuged for 15 min at 14,000 g. The supernatant was mixed with 100% ethanol (1/3 v/v) and RNA was extracted using the RNA extraction kit (NucleoSpin® RNA kit, Macherey-Nagel) according to the manufacturer’s instructions. .. RNA purity and concentration was determined using the NanoDrop ND-1000 (PeqLab). cDNA synthesis was performed with 400 ng RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random hexamer primers following the manufacturer's instructions. .. Relative mRNA levels were determined by semiquantitative RT-PCR (qRT-PCR) using the TaqMan Gene Expression Assay Technique and the 7900HT Fast Real-Time PCR System (Applied Biosystems).

Article Title: ZCCHC13-mediated induction of human liver cancer is associated with the modulation of DNA methylation and the AKT/ERK signaling pathway
Article Snippet: Total RNA was extracted from human cell lines using the TRIzol Reagent according to the manufacturer’s protocol. .. The concentration and quality of RNA were measured and confirmed using a NanoDrop ND-1000 (Peqlab, Erlangen, Germany) and electrophoresis, respectively. .. The cDNA templates were synthesized using Quanti-Tect Reverse Transcription Kit (Qiagen, Hilden, Germany).

Article Title: Interaction of two antitumor peptides with membrane lipids – Influence of phosphatidylserine and cholesterol on specificity for melanoma cells
Article Snippet: Stock solutions of R-DIM-P-LF11-322 and DIM-LF11-318 were prepared in acetic acid (0.1% v/v) at a concentration of about 3 mg/ml and treated with ultrasonication for better solubility. .. The peptide concentrations of the non-labeled peptides were determined via measurement of UV-absorbance of tryptophan at 280 nm (using NanoDrop ND 1000 (Peqlab, VWR International, Inc. Erlangen, Germany)).

Article Title: The first evidence of a new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and unvaccinated broiler flocks in Algeria
Article Snippet: The Trifast FL kit (Peq GOLD TriFast™ FL, Peqlab) was used to extract the RNA from the FTA® paper according to the manufacturer’s instructions (PEQLAB Biotechnologie GmbH, Erlangen, Germany). .. RNA purity and concentration were determined by NanoDrop® ND–1000 (Peqlab Biotechnologie GmbH, Erlangen, Germany). .. Reverse transcription was performed using the ImProm-II™ Reverse Transcription System (Promega, Madison, WI) with random primers according to the manufacturer’s instructions.

Article Title: Temperature effects on gene expression and morphological development of European eel, Anguilla anguilla larvae
Article Snippet: After obtaining the aqueous phase by incubation in 160 μl chloroform, RNA was extracted using the InviTrap® Spin tissue RNA MiniKit (STRATEC Biomedical AG, Berlin-Buch, Germany) following the manufacturer’s instructions. .. RNA concentration (764 ± 60 ng μl-1 ) and purity (260/280 = 2.12 ± 0.16, 230/260 = 2.16 ± 0.16) were determined by spectrophotometry using Nanodrop ND-1000 (Peqlab, Germany). .. From the resulting total RNA, 680 ng were transcribed using the Quanta qScript cDNA Synthesis Kit (Promega, Germany) according to the manufacturer’s instructions, including an additional gDNA wipe out step prior to transcription [Quanta PerfeCta DNase I Kit (Promega, Germany)].

Article Title: 3,5-Diiodo-L-Thyronine (3,5-T2) Exerts Thyromimetic Effects on Hypothalamus-Pituitary-Thyroid Axis, Body Composition, and Energy Metabolism in Male Diet-Induced Obese Mice
Article Snippet: Total mRNA from pituitaries was isolated using the Aurum kit (Bio-Rad) following manufacturer's guidelines. .. Purity and concentration were determined with a NanoDrop ND-1000 (Peqlab). .. Approximately 500 ng/μL of total RNA was required to synthesize cDNA using the iScript cDNA synthesis kit (Bio-Rad).

Article Title: CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells
Article Snippet: To this end, the medium was removed and the cells were fixed with 4% paraformaldehyde (20 min) and stained with Alizarin S Red (Sigma Aldrich) (10 min). .. Total RNA was extracted using Arcturus™ PicoPure™ RNA Isolation Kit (Applied Biosystems, Darmstadt, Germany) according to the manufacturer’s instructions and the RNA concentration was determined using Nanodrop ND-1000 (Peqlab Biotechnologie, Erlangen, Germany). .. RNA was stored at −80 °C until further processing.

Article Title: Validation of Deleterious Mutations in Vorderwald Cattle
Article Snippet: Genomic DNA was isolated from EDTA-blood samples using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1% agarose gels (peqGold Universal Agarose, Peqlab Biotechnology). .. After quality control (genotyping rate per SNP and animal > 0.98), filtering for Hardy-Weinberg equilibrium (HWE) (p < 0.000001) using SAS/Genetics, version 9.4 (Statistical Analysis System, SAS-Institute, Cary, NE, USA), 44,811 SNPs were left for the analysis.

Article Title: Genome-wide association study for hereditary ataxia in the Parson Russell Terrier and DNA-testing for ataxia-associated mutations in the Parson and Jack Russell Terrier
Article Snippet: Genomic DNA was isolated using standard methods with RBC (Red Blood Cell) lysis buffer and SE (sodium EDTA) buffer. .. The DNA concentration of the samples was adjusted to 50 ng/μl using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany) and quality control was performed by gel electrophoresis using 1 % agarose gels (peqGold Universal Agarose, Peqlab Biotechnologie). .. We performed a GWAS in 44 PRT using the canine Illumina high density beadchip (Illumina, San Diego, CA, USA) containing 173,662 single nucleotide polymorphisms (SNPs).

Article Title: Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation
Article Snippet: Further treatment of samples was according to the manufacturer’s instructions, including a DNAse I treatment on the column. .. RNA was eluted in 100 μl nuclease free water and the concentration was determined using a Nanodrop ND 1000 (PeqLab, Germany). .. Integrity of RNA was evaluated using a Bioanalyzer 2100 (Agilent, Germany).

Article Title: Germline mutation within COL2A1 associated with lethal chondrodysplasia in a polled Holstein family
Article Snippet: Genomic DNA was extracted from the EDTA-blood samples using routine procedures. .. Concentration of extracted DNA was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. DNA concentration was adjusted to 50–80 ng/μl.

Article Title: A Genome-Wide Association Study Identifies Risk Loci to Equine Recurrent Uveitis in German Warmblood Horses
Article Snippet: The DNA concentration of the samples was adjusted to 50 ng/µl. .. DNA concentration was determined using the Nanodrop ND-1000 (Peqlab Biotechnology, Erlangen, Germany). .. Genotyping was performed using the Illumina equine SNP50 beadchip including 54,602 SNPs (single nucleotide polymorphisms) using standard procedures as recommended by the manufacturer.

Staining:

Article Title: Metronomic temozolomide as second line treatment for metastatic poorly differentiated pancreatic neuroendocrine carcinoma
Article Snippet: To ensure high tumour DNA content, FFPE tissue sections were stained with H & E and histologically examined by an expert neuropathologist Sections showing a tumour cell content of more than 80 % were directly subjected to DNA extraction.. .. Extraction of genomic DNA was performed using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) and quantified with a NanoDrop ND-1000 (PeqLab, Erlangen, Germany).

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    PEQLAB nd 1000 spectrophotometer
    Nd 1000 Spectrophotometer, supplied by PEQLAB, used in various techniques. Bioz Stars score: 97/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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