nd 1000 spectrophometer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nd 1000 spectrophometer
    Nd 1000 Spectrophometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 spectrophometer/product/Thermo Fisher
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nd 1000 spectrophometer - by Bioz Stars, 2019-12
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: Paragraph title: Cloning and protein expression ... Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Amplification:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer. .. Real-time qPCR was performed with the Roche LightCycler 480 RT PCR Instrument using SYBR Green Mastermix (Applied Biosystems) and the default two-step QRT-PCR program.

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: From each pool 1 μg of total RNA was converted into labeled cRNA with nucleotides coupled to a fluorescent dye (either Cy3 or Cy5) using the Low RNA Input Linear Amplification Kit (Agilent Technologies, Palo Alto, CA, USA) following the manufacturer's protocol. .. The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer.

    Neutralization:

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: For peptide-induced effects cells were treated with RPMI-1640 medium containing 20 μM peptide during 4 h or 24 h. In LPS neutralization experiments, final concentrations of 50–100 ng/ml LPS were pre-incubated with or without 20 μM peptide for 30 min at 37°C (5% CO2 ), applied to the cells and incubated for 4 h. To examine neutralization of LPS-primed cells HD11 cells were exposed for 30 min to 100–1000 ng/ml S . minnesota LPS, washed once and incubated during 4 h in the absence or presence of 20 μM Peptide. .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Cytometry:

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Quantitative real-time PCR array RNA was extracted from flow cytometry-sorted lin− /CD34+(low) /CD31− cells with the PicoPure RNA Isolation kit (Arcturus, Sunnyvale, CA, USA, no. KIT0204) according to manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Quantitative RT-PCR:

    Article Title: Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder
    Article Snippet: RNA samples (1–2 µg) from frontal cortex (BA9; 33 control and 31 BD samples), anterior cingulate cortex (29 control and 29 BD samples) and cerebellum (lateral cerebellar hemisphere; 34 control and 29 BD samples) were obtained from the Stanley Medical Research Institute (Chevy Chase, MD, USA) Array Collection. .. Quality and concentration of total RNA was measured using a NanoDrop ND-1000 spectrophometer (Thermo Fisher Scientific, Waltham, MA) and each sample was processed in triplicate for quantification of miR-34a and miR-34a target expression by quantitative real time PCR (qRT-PCR). miR-708 levels were measured in cerebellar samples only. .. The research met the criteria for Exemption 4 in terms of Human Subjects Research and was approved by the Institutional Review Board of the Massachusetts General Hospital.

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    SYBR Green Assay:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Microarray:

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: Paragraph title: Microarray analysis ... The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer.

    Incubation:

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: Three HEK293 derivatives expressing either none (vector), wtAPE1, or Ub-APE1 fusion linked at APE1's 24th Lys, were incubated with/without doxycycline (dox) 2 µg/ml for 16 h. Each −/+ dox cultures were triplicated. .. The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: For peptide-induced effects cells were treated with RPMI-1640 medium containing 20 μM peptide during 4 h or 24 h. In LPS neutralization experiments, final concentrations of 50–100 ng/ml LPS were pre-incubated with or without 20 μM peptide for 30 min at 37°C (5% CO2 ), applied to the cells and incubated for 4 h. To examine neutralization of LPS-primed cells HD11 cells were exposed for 30 min to 100–1000 ng/ml S . minnesota LPS, washed once and incubated during 4 h in the absence or presence of 20 μM Peptide. .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Activity Assay:

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE). .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Senescence Cells Histochemical Staining Kit (Sigma, St. Louis, MO, USA, no. CS00030) was used to stain the cells for senescence associated β-galactosidase activity according to the manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Expressing:

    Article Title: Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder
    Article Snippet: RNA samples (1–2 µg) from frontal cortex (BA9; 33 control and 31 BD samples), anterior cingulate cortex (29 control and 29 BD samples) and cerebellum (lateral cerebellar hemisphere; 34 control and 29 BD samples) were obtained from the Stanley Medical Research Institute (Chevy Chase, MD, USA) Array Collection. .. Quality and concentration of total RNA was measured using a NanoDrop ND-1000 spectrophometer (Thermo Fisher Scientific, Waltham, MA) and each sample was processed in triplicate for quantification of miR-34a and miR-34a target expression by quantitative real time PCR (qRT-PCR). miR-708 levels were measured in cerebellar samples only. .. The research met the criteria for Exemption 4 in terms of Human Subjects Research and was approved by the Institutional Review Board of the Massachusetts General Hospital.

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: Paragraph title: Cloning and protein expression ... Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma
    Article Snippet: Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA). .. Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA).

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: Paragraph title: Total RNA extraction and gene expression array profiling ... The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    Hybridization:

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent). .. The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer. .. Data was extracted from the scanned image using Feature Extraction v. 7.1 or 7.5 (Agilent Technologies; Redwood City, CA, USA) and analyzed using GeneSpring v. 7.2 software (Agilent Technologies).

    High Performance Liquid Chromatography:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: The protein sample was then injected into a g2000sw molecular sieving column (Tosoh Biosciences) with a 1 ml loop connected to the HPLC (DIONEX) with a phosphate buffer (PBS) pH 7.2 as the buffer for further purification. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: The protein sample was then injected into a g2000sw molecular sieving column (Tosoh Biosciences) with a 1 ml loop connected to HPLC (DIONEX) with phosphate buffer (PBS) pH 7.2 as the buffer for further purification. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Flow Cytometry:

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Quantitative real-time PCR array RNA was extracted from flow cytometry-sorted lin− /CD34+(low) /CD31− cells with the PicoPure RNA Isolation kit (Arcturus, Sunnyvale, CA, USA, no. KIT0204) according to manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Cell Culture:

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: Paragraph title: Cell culture ... RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Senescence associated β-galactosidase assay Each well of a 24-well plate was seeded with 2 × 103 lin− SVF in growth media (DMEM/F12 with10 mM HEPES, 10% FBS, and 1% penicillin/streptomycin) and cultured for 21 days under standard culture conditions (37°C, 5% CO2 ). .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Injection:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: The protein sample was then injected into a g2000sw molecular sieving column (Tosoh Biosciences) with a 1 ml loop connected to the HPLC (DIONEX) with a phosphate buffer (PBS) pH 7.2 as the buffer for further purification. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: The protein sample was then injected into a g2000sw molecular sieving column (Tosoh Biosciences) with a 1 ml loop connected to HPLC (DIONEX) with phosphate buffer (PBS) pH 7.2 as the buffer for further purification. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Recombinant:

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: Paragraph title: Production and purification of recombinant PpSp30 and PpSp32 proteins (rPpSp30 and rPpSp32) ... Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Molecular Weight:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: Appropriate fractions as determined by molecular weight compared to a standard in the gel were pooled and concentrated to 1 ml using a 10 kDa Amicon Ultra Centrifugal Filter (Millipore). .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: Appropriate fractions as determined by molecular weight compared to standard in gel were pooled and concentrated to 1 ml using a 10 kDa Amicon Ultra Centrifugal Filter (Millipore). .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    DNA Extraction:

    Article Title: Genome-Wide Association Scan of Dupuytren's Disease
    Article Snippet: Paragraph title: Genomic DNA Extraction ... The extracted DNA was evaluated for quality using NanoDrop ND-1000 Spectrophometer (Fisher/Thermo Scientific).

    Isolation:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Cells were stimulated as described in triplicate and RNA was isolated using the RNeasy Mini kit (Qiagen) following manufacturer’s instructions. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Article Title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma
    Article Snippet: RNA from mentioned 17 pairs of tissue blocks were extracted using TRIzol solution (Invitrogen, USA) according to the manufacturer’s instruction. .. Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA). .. The RNA underwent reverse transcription with the program of 30 min at 16°C, 30 min at 42°C and 5 min at 85°C.

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: Remaining cells were lysed in lysis buffer and total RNA was isolated and purified using a High Pure RNA isolation kit (Roche, Mannheim, Germany) according the manufacturer's recommendations. .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Quantitative real-time PCR array RNA was extracted from flow cytometry-sorted lin− /CD34+(low) /CD31− cells with the PicoPure RNA Isolation kit (Arcturus, Sunnyvale, CA, USA, no. KIT0204) according to manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Labeling:

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent). .. The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: From each pool 1 μg of total RNA was converted into labeled cRNA with nucleotides coupled to a fluorescent dye (either Cy3 or Cy5) using the Low RNA Input Linear Amplification Kit (Agilent Technologies, Palo Alto, CA, USA) following the manufacturer's protocol. .. The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer. .. Equal amounts of Cy3 and Cy5-labeled cRNA (750 ng) from two different samples were hybridized to mouse microarrays (Agilent Mouse Oligo Microarrays, G4121A) for 17 h at 60°C.

    Purification:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: The protein sample was then injected into a g2000sw molecular sieving column (Tosoh Biosciences) with a 1 ml loop connected to the HPLC (DIONEX) with a phosphate buffer (PBS) pH 7.2 as the buffer for further purification. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: Paragraph title: Production and purification of recombinant PpSp30 and PpSp32 proteins (rPpSp30 and rPpSp32) ... Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: Remaining cells were lysed in lysis buffer and total RNA was isolated and purified using a High Pure RNA isolation kit (Roche, Mannheim, Germany) according the manufacturer's recommendations. .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

    Article Title: Homeostasis of lymphocytes and monocytes in frequent blood donors
    Article Snippet: In brief, genomic DNA was purified from CD4 and CD8 T cells using a Gentra Puregene Cell Kit according to the manufacturer’s instructions (Gentra Systems, Inc., Minneapolis, MN,USA). .. DNA levels were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and further normalized using the house keeping gene RPL-32.

    Polymerase Chain Reaction:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Paragraph title: PCR and primers ... Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA). .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Staining:

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: Afterwards, the gel was stained with Coomassie Blue (0.025%) to visualize proteins. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: After run, gel was stained with Coomassie Blue (0.025%) to visualize proteins. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein.

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent). .. All experiments were performed using GeneChip Exon 1.0 ST Arrays and Whole Transcript Sense Target labeling Assay (version 4, Affymetrix).

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Senescence Cells Histochemical Staining Kit (Sigma, St. Louis, MO, USA, no. CS00030) was used to stain the cells for senescence associated β-galactosidase activity according to the manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    Plasmid Preparation:

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: Three HEK293 derivatives expressing either none (vector), wtAPE1, or Ub-APE1 fusion linked at APE1's 24th Lys, were incubated with/without doxycycline (dox) 2 µg/ml for 16 h. Each −/+ dox cultures were triplicated. .. The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    Software:

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer. .. The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer.

    Real-time Polymerase Chain Reaction:

    Article Title: Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder
    Article Snippet: RNA samples (1–2 µg) from frontal cortex (BA9; 33 control and 31 BD samples), anterior cingulate cortex (29 control and 29 BD samples) and cerebellum (lateral cerebellar hemisphere; 34 control and 29 BD samples) were obtained from the Stanley Medical Research Institute (Chevy Chase, MD, USA) Array Collection. .. Quality and concentration of total RNA was measured using a NanoDrop ND-1000 spectrophometer (Thermo Fisher Scientific, Waltham, MA) and each sample was processed in triplicate for quantification of miR-34a and miR-34a target expression by quantitative real time PCR (qRT-PCR). miR-708 levels were measured in cerebellar samples only. .. The research met the criteria for Exemption 4 in terms of Human Subjects Research and was approved by the Institutional Review Board of the Massachusetts General Hospital.

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Article Title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR ... Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA).

    Article Title: Homeostasis of lymphocytes and monocytes in frequent blood donors
    Article Snippet: The frequency of the δRec-ψJα TREC created when the TCRD segment is excised from the TCR β gene locus was determined using Taqman real-time PCR 7500 Fast Real-Time PCR System (Applied Biosystems). .. DNA levels were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and further normalized using the house keeping gene RPL-32.

    Article Title: Caloric restriction attenuates the age-associated increase of adipose-derived stem cells but further reduces their proliferative capacity
    Article Snippet: Quantitative real-time PCR array RNA was extracted from flow cytometry-sorted lin− /CD34+(low) /CD31− cells with the PicoPure RNA Isolation kit (Arcturus, Sunnyvale, CA, USA, no. KIT0204) according to manufacturer's instructions. .. RNA concentrations were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop, Wilmington, DE, USA).

    RNA Extraction:

    Article Title: Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder
    Article Snippet: Paragraph title: Postmortem human brain sample collection and RNA extraction ... Quality and concentration of total RNA was measured using a NanoDrop ND-1000 spectrophometer (Thermo Fisher Scientific, Waltham, MA) and each sample was processed in triplicate for quantification of miR-34a and miR-34a target expression by quantitative real time PCR (qRT-PCR). miR-708 levels were measured in cerebellar samples only.

    Article Title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma
    Article Snippet: Paragraph title: RNA extraction and quantitative PCR ... Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA).

    Article Title: Ubiquitination of human AP-endonuclease 1 (APE1) enhanced by T233E substitution and by CDK5
    Article Snippet: Paragraph title: Total RNA extraction and gene expression array profiling ... The quality and quantity of total RNA were assessed using ND-1000 Spectrophometer (NanoDrop, Wilmington, DE), NanoChip assay and Bioanalyzer 2100 (Agilent).

    RNA Expression:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Quantitation of RNA expression was performed by realtime PCR. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Transgenic Assay:

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: Additional file summarizes the pooling scheme from wild-type and transgenic mammary glands and two of the three tumor types and shows the microarray hybridizations performed. .. The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer.

    Quantitation Assay:

    Article Title: Presenilin1 regulates Th1 and Th17 effector responses but is not required for experimental autoimmune encephalomyelitis
    Article Snippet: Quantitation of RNA expression was performed by realtime PCR. .. Total RNA concentrations were measured using NanoDrop ND-1000 spectrophometer.

    Article Title: Homeostasis of lymphocytes and monocytes in frequent blood donors
    Article Snippet: A TREC standard was used for quantitation (gift from Dr. Daniel C. Douek, National Institute of Allergy and Infectious Diseases, NIH) as previously described ( ). .. DNA levels were determined using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and further normalized using the house keeping gene RPL-32.

    Concentration Assay:

    Article Title: Dysregulation of miR-34a Links Neuronal Development to Genetic Risk Factors for Bipolar Disorder
    Article Snippet: RNA samples (1–2 µg) from frontal cortex (BA9; 33 control and 31 BD samples), anterior cingulate cortex (29 control and 29 BD samples) and cerebellum (lateral cerebellar hemisphere; 34 control and 29 BD samples) were obtained from the Stanley Medical Research Institute (Chevy Chase, MD, USA) Array Collection. .. Quality and concentration of total RNA was measured using a NanoDrop ND-1000 spectrophometer (Thermo Fisher Scientific, Waltham, MA) and each sample was processed in triplicate for quantification of miR-34a and miR-34a target expression by quantitative real time PCR (qRT-PCR). miR-708 levels were measured in cerebellar samples only. .. The research met the criteria for Exemption 4 in terms of Human Subjects Research and was approved by the Institutional Review Board of the Massachusetts General Hospital.

    Article Title: Molecular Diversity between Salivary Proteins from New World and Old World Sand Flies with Emphasis on Bichromomyia olmeca, the Sand Fly Vector of Leishmania mexicana in Mesoamerica
    Article Snippet: Appropriate fractions were determined as described above and pooled. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein. .. All SPR experiments were carried out in a T100 instrument (Biacore Inc., Uppsala, Sweden) following the manufacturer's instructions.

    Article Title: Salivary Antigen SP32 Is the Immunodominant Target of the Antibody Response to Phlebotomus papatasi Bites in Humans
    Article Snippet: Appropriate fractions were determined as described above and pooled. .. Concentration was measured by using a NanoDrop ND-1000 spectrophometer at 280 nm and calculated using the extinction coefficient of the protein. .. IgG antibodies against recombinant forms of PpSP30 and PpSP32 as well as against total salivary gland extract (SGE) were measured by ELISA (Enzyme-Linked Immunosorbent Assay).

    Article Title: High expression of N-acetyltransferase 10: a novel independent prognostic marker of worse outcome in patients with hepatocellular carcinoma
    Article Snippet: RNA from mentioned 17 pairs of tissue blocks were extracted using TRIzol solution (Invitrogen, USA) according to the manufacturer’s instruction. .. Isolated RNA concentration was then determined using the NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, USA). .. The RNA underwent reverse transcription with the program of 30 min at 16°C, 30 min at 42°C and 5 min at 85°C.

    Standard Deviation:

    Article Title: Expression of truncated Int6/eIF3e in mammary alveolar epithelium leads to persistent hyperplasia and tumorigenesis
    Article Snippet: The quality and quantity of the resulting labeled cRNA was assessed using a NanoDrop ND-1000 spectrophometer (NanoDrop Technologies, Wilmington, DE, USA) and an Agilent 2100 Bioanalyzer. .. Data was extracted from the scanned image using Feature Extraction v. 7.1 or 7.5 (Agilent Technologies; Redwood City, CA, USA) and analyzed using GeneSpring v. 7.2 software (Agilent Technologies).

    Lysis:

    Article Title: Immunomodulatory and Anti-Inflammatory Activities of Chicken Cathelicidin-2 Derived Peptides
    Article Snippet: Remaining cells were lysed in lysis buffer and total RNA was isolated and purified using a High Pure RNA isolation kit (Roche, Mannheim, Germany) according the manufacturer's recommendations. .. RNA quantity and purity were tested using a Nanodrop ND-1000 spectrophometer (Nanodrop Technologies, Wilmington, DE).

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  • 99
    Thermo Fisher nd 1000 spectrophotometer
    Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3704 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3704 article reviews
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    95
    Thermo Fisher nd 1000 spectrophometer
    Nd 1000 Spectrophometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 spectrophometer/product/Thermo Fisher
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    Thermo Fisher nanodrop nd 1000 spectrophotometer
    Nanodrop Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrophotometer/product/Thermo Fisher
    Average 95 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2019-12
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