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LabTech Inc nanodrop nd 1000 spectrophotometer
Nanodrop Nd 1000 Spectrophotometer, supplied by LabTech Inc, used in various techniques. Bioz Stars score: 96/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 66 article reviews
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nanodrop nd 1000 spectrophotometer - by Bioz Stars, 2020-01
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DNA Extraction:

Article Title: Common FXIII and Fibrinogen Polymorphisms in Abdominal Aortic Aneurysms
Article Snippet: Paragraph title: Blood sampling and DNA extraction ... DNA concentration was measured using the 260/280 nm optical density ratio using a Nanodrop ND-1000 spectrophotometer (LabTech International, Uckfield, UK).

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: Paragraph title: DNA extraction and PCR amplification ... The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA.

Article Title: Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat
Article Snippet: Paragraph title: Growth conditions and DNA extraction ... DNA yield and purity (absorbance ratio at 260/280 nm) were determined using a NanoDrop ND-1000 spectrophotometer (Labtech, Palaiseau, France).

Centrifugation:

Article Title: Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children
Article Snippet: Chloroform was then added and after centrifugation RNA was recovered in the aqueous phase and precipitated by addition of isopropyl alcohol. .. RNA was then resuspended in RNAse-free H2 0 and RNA concentration was determined by spectrophotometry on a NanoDrop ND-1000 spectrophotometer (Labtech, France).

Amplification:

Article Title: Analysis of prognosis, genome, microbiome, and microbial metabolome in different sites of colorectal cancer
Article Snippet: Paragraph title: PCR amplification of bacterial 16S rDNA and fungal ITS rDNA ... The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with absorbances at 260 nm and 280 nm (A260/A280) was used to detect the purity of DNA.

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France). .. PCR amplification, multiplexing, and genotyping were carried out following Delêtre et al. ( ).

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: Paragraph title: DNA extraction and PCR amplification ... The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA.

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France). .. The melting curve of the PCR products was analysed at each time point to ensure amplification specificity.

Quantitative RT-PCR:

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: Paragraph title: RNA isolation and RT-qPCR of the cytokines and chemokines ... RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK).

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. The qRT-PCR analysis of cry5Ba and BtsR1 was carried out using two-step methods with the primers listed in .

SYBR Green Assay:

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK). .. Quantitative PCR reaction was performed with cDNA template derived from 4 ng of total RNA in triplicate using SYBR Green Master mix (Applied Biosystems, ThermoFisher Scientific).

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. 2 μg total RNA from each sample was used for cDNA synthesis with Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, CA, USA). qRT-PCR was then conducted with Power SYBR Green PCR Master Mix in an Applied Biosystems 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA).

Incubation:

Article Title: STIM and Orai isoform expression in pregnant human myometrium: a potential role in calcium signaling during pregnancy
Article Snippet: The cells were then aspirated to dryness and 100 μl of lysis buffer was added to each well and incubated on ice for 5 min. .. Tissue and whole cell lysate protein content was quantified using a NanoDrop ND-1000 spectrophotometer (Labtech).

Infection:

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: Paragraph title: DNA-based quantification of P. brassicae in infected tissues ... The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France).

Western Blot:

Article Title: STIM and Orai isoform expression in pregnant human myometrium: a potential role in calcium signaling during pregnancy
Article Snippet: Paragraph title: Identification of STIM1 and Orai1 in myometrial tissue and cultured uterine smooth muscle cells by western blotting ... Tissue and whole cell lysate protein content was quantified using a NanoDrop ND-1000 spectrophotometer (Labtech).

Real-time Polymerase Chain Reaction:

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK). .. Quantitative PCR reaction was performed with cDNA template derived from 4 ng of total RNA in triplicate using SYBR Green Master mix (Applied Biosystems, ThermoFisher Scientific).

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. 2 μg total RNA from each sample was used for cDNA synthesis with Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, CA, USA). qRT-PCR was then conducted with Power SYBR Green PCR Master Mix in an Applied Biosystems 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA).

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France). .. Real-time quantitative PCR was performed in a Light Cycler 480 (Roche Diagnostics GmbH, Mannheim, Germany) as described by .

Derivative Assay:

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK). .. Quantitative PCR reaction was performed with cDNA template derived from 4 ng of total RNA in triplicate using SYBR Green Master mix (Applied Biosystems, ThermoFisher Scientific).

Protease Inhibitor:

Article Title: STIM and Orai isoform expression in pregnant human myometrium: a potential role in calcium signaling during pregnancy
Article Snippet: Identification of STIM1 and Orai1 in myometrial tissue and cultured uterine smooth muscle cells by western blotting Human myometrial tissues were homogenized using a Tissue Lyser II (Qiagen) at 25 Hz for 3 min in 20 μl lysis buffer/mg of tissue [10 mM of HEPES-KOH (pH 7); 1 mM of dithiothreitol; 1% nonident-P40, and protease-inhibitor cocktail (COMPLETE tablets, Boehringer-Mannheim Biochemical, Lewes, Sussex, UK)]. .. Tissue and whole cell lysate protein content was quantified using a NanoDrop ND-1000 spectrophotometer (Labtech).

Cell Culture:

Article Title: STIM and Orai isoform expression in pregnant human myometrium: a potential role in calcium signaling during pregnancy
Article Snippet: Paragraph title: Identification of STIM1 and Orai1 in myometrial tissue and cultured uterine smooth muscle cells by western blotting ... Tissue and whole cell lysate protein content was quantified using a NanoDrop ND-1000 spectrophotometer (Labtech).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: Total RNA extraction Strains for transcriptome analysis were cultured to log phase (OD600 = 0.3), after which 1 ml of culture of each strain was transferred to 100 ml fresh SCVy medium in 250-ml flasks. .. The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA).

DNA Sequencing:

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Paragraph title: Sampling, genotyping, and DNA sequencing ... Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France).

Polymerase Chain Reaction:

Article Title: Analysis of prognosis, genome, microbiome, and microbial metabolome in different sites of colorectal cancer
Article Snippet: Paragraph title: PCR amplification of bacterial 16S rDNA and fungal ITS rDNA ... The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with absorbances at 260 nm and 280 nm (A260/A280) was used to detect the purity of DNA.

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France). .. PCR amplification, multiplexing, and genotyping were carried out following Delêtre et al. ( ).

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: Paragraph title: DNA extraction and PCR amplification ... The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA.

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. 2 μg total RNA from each sample was used for cDNA synthesis with Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, CA, USA). qRT-PCR was then conducted with Power SYBR Green PCR Master Mix in an Applied Biosystems 7300 Real Time PCR System (Applied Biosystems, Foster City, CA, USA).

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France). .. The melting curve of the PCR products was analysed at each time point to ensure amplification specificity.

Staining:

Article Title: Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat
Article Snippet: DNA yield and purity (absorbance ratio at 260/280 nm) were determined using a NanoDrop ND-1000 spectrophotometer (Labtech, Palaiseau, France). .. DNA integrity was verified by electrophoresis in a 1% agarose gel (Qbiogene, Illkirch, France) in 1X TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) stained with SYBR® Safe 1X (Invitrogen, Carlsbad, CA, USA).

Multiplexing:

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France). .. PCR amplification, multiplexing, and genotyping were carried out following Delêtre et al. ( ).

Multiple Displacement Amplification:

Article Title: The proteomes of transcription factories containing RNA polymerases I, II or III
Article Snippet: The amount of protein in the area of a gel containing three complexes ( – dotted oval) was measured by densitometry using AIDA software and blue carrier immunogenic protein (8 MDa; Pierce) as a standard. .. Protein concentrations were monitored using a Nanodrop ND-1000 spectrophotometer (LabTech).

Isolation:

Article Title: Balance between Estrogens and Proinflammatory Cytokines Regulates Chemokine Production Involved in Thymic Germinal Center Formation
Article Snippet: Total RNA was prepared from the thymus and TECs using the trizol RNA Isolation kit (Invitrogen, Cergy-Pontoise, France). .. The quality and concentration of RNA were analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France).

Article Title: Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality
Article Snippet: RNA Extraction Total RNA was prepared from the thymus and TECs using the trizol RNA Isolation kit (Life Technologies, Cergy-Pontoise, France). .. The concentration of RNA was analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France).

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: Paragraph title: RNA isolation and RT-qPCR of the cytokines and chemokines ... RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: Then, total RNA was isolated using the Trizol reagent (Invitrogen, Carlsbad, CA, USA) from exponentially growing Sulfolobus cultures (OD600 = 0.3) in SCVy medium for moderate induction of the csa3a gene under control of the araS promoter, as described previously ( ). .. The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA).

Purification:

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: .. Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France). .. Genetic diversity was analyzed using the 17 nuclear Simple Sequence Repeats (SSRs) designed by Delêtre, Soengas, Utge, Lambourdière, and Sørensen ( ).

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: .. The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA. .. DNA integrity was further verified by electrophoresis through a 2.0% (w/v) agarose gel.

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: Tissue samples were homogenized in a lysis buffer with 2.8-mm ceramic beads (MO BIO Laboratories Inc., Carlsbad, USA) using TissueLyser II (Qiagen, Hilden, DE) prior to subsequent purification as described in the protocol. .. RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: .. The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. Total RNA integrity was verified by electrophoresis on a 1.5% agarose gel.

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: .. The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. The qRT-PCR analysis of cry5Ba and BtsR1 was carried out using two-step methods with the primers listed in .

Sequencing:

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France). .. Genetic diversity was analyzed using the 17 nuclear Simple Sequence Repeats (SSRs) designed by Delêtre, Soengas, Utge, Lambourdière, and Sørensen ( ).

Lysis:

Article Title: STIM and Orai isoform expression in pregnant human myometrium: a potential role in calcium signaling during pregnancy
Article Snippet: The cells were then aspirated to dryness and 100 μl of lysis buffer was added to each well and incubated on ice for 5 min. .. Tissue and whole cell lysate protein content was quantified using a NanoDrop ND-1000 spectrophotometer (Labtech).

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: Tissue samples were homogenized in a lysis buffer with 2.8-mm ceramic beads (MO BIO Laboratories Inc., Carlsbad, USA) using TissueLyser II (Qiagen, Hilden, DE) prior to subsequent purification as described in the protocol. .. RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK).

Software:

Article Title: The proteomes of transcription factories containing RNA polymerases I, II or III
Article Snippet: The amount of protein in the area of a gel containing three complexes ( – dotted oval) was measured by densitometry using AIDA software and blue carrier immunogenic protein (8 MDa; Pierce) as a standard. .. Protein concentrations were monitored using a Nanodrop ND-1000 spectrophotometer (LabTech).

Electrophoresis:

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA. .. DNA integrity was further verified by electrophoresis through a 2.0% (w/v) agarose gel.

Article Title: Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat
Article Snippet: DNA yield and purity (absorbance ratio at 260/280 nm) were determined using a NanoDrop ND-1000 spectrophotometer (Labtech, Palaiseau, France). .. DNA integrity was verified by electrophoresis in a 1% agarose gel (Qbiogene, Illkirch, France) in 1X TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) stained with SYBR® Safe 1X (Invitrogen, Carlsbad, CA, USA).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. Total RNA integrity was verified by electrophoresis on a 1.5% agarose gel.

RNA Extraction:

Article Title: Balance between Estrogens and Proinflammatory Cytokines Regulates Chemokine Production Involved in Thymic Germinal Center Formation
Article Snippet: Paragraph title: RNA extraction and reverse transcription ... The quality and concentration of RNA were analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France).

Article Title: Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality
Article Snippet: Paragraph title: RNA Extraction ... The concentration of RNA was analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France).

Article Title: Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children
Article Snippet: Paragraph title: RNA extraction ... RNA was then resuspended in RNAse-free H2 0 and RNA concentration was determined by spectrophotometry on a NanoDrop ND-1000 spectrophotometer (Labtech, France).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: Paragraph title: Total RNA extraction ... The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA).

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: Paragraph title: RNA extraction and transcription analyses ... The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA).

Agarose Gel Electrophoresis:

Article Title: Balance between Estrogens and Proinflammatory Cytokines Regulates Chemokine Production Involved in Thymic Germinal Center Formation
Article Snippet: The quality and concentration of RNA were analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France). .. RNA samples presenting a minimal ratio of 1.9 and 2 for respectively 260/280 and 260/230 were also controlled on a denaturing agarose gel.

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA. .. DNA integrity was further verified by electrophoresis through a 2.0% (w/v) agarose gel.

Article Title: Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat
Article Snippet: DNA yield and purity (absorbance ratio at 260/280 nm) were determined using a NanoDrop ND-1000 spectrophotometer (Labtech, Palaiseau, France). .. DNA integrity was verified by electrophoresis in a 1% agarose gel (Qbiogene, Illkirch, France) in 1X TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) stained with SYBR® Safe 1X (Invitrogen, Carlsbad, CA, USA).

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. Total RNA integrity was verified by electrophoresis on a 1.5% agarose gel.

Spectrophotometry:

Article Title: Analysis of prognosis, genome, microbiome, and microbial metabolome in different sites of colorectal cancer
Article Snippet: .. The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with absorbances at 260 nm and 280 nm (A260/A280) was used to detect the purity of DNA. ..

Article Title: Common FXIII and Fibrinogen Polymorphisms in Abdominal Aortic Aneurysms
Article Snippet: .. DNA concentration was measured using the 260/280 nm optical density ratio using a Nanodrop ND-1000 spectrophotometer (LabTech International, Uckfield, UK). .. Real Time Polymerase Chain Reaction TaqMan single nucleotide polymorphism (SNP) Genotyping Assays (Life Technologies, Paisley, UK) were used to identify each of the polymorphisms (FXIII A Val34Leu (rs5985), FXIII B His95Arg (rs6003), Splice Variant (intron K nt29576C-G) (rs12134960), Fib-β Arg448Lys (rs4220) and Fib-α Thr312Ala (rs6050)).

Article Title: Balance between Estrogens and Proinflammatory Cytokines Regulates Chemokine Production Involved in Thymic Germinal Center Formation
Article Snippet: .. The quality and concentration of RNA were analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France). .. RNA samples presenting a minimal ratio of 1.9 and 2 for respectively 260/280 and 260/230 were also controlled on a denaturing agarose gel.

Article Title: Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality
Article Snippet: .. The concentration of RNA was analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France). ..

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: .. The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA. .. DNA integrity was further verified by electrophoresis through a 2.0% (w/v) agarose gel.

Article Title: Comparative genomic analysis of Brevibacterium strains: insights into key genetic determinants involved in adaptation to the cheese habitat
Article Snippet: .. DNA yield and purity (absorbance ratio at 260/280 nm) were determined using a NanoDrop ND-1000 spectrophotometer (Labtech, Palaiseau, France). .. DNA integrity was verified by electrophoresis in a 1% agarose gel (Qbiogene, Illkirch, France) in 1X TAE buffer (40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH 8.3) stained with SYBR® Safe 1X (Invitrogen, Carlsbad, CA, USA).

Article Title: Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children
Article Snippet: .. RNA was then resuspended in RNAse-free H2 0 and RNA concentration was determined by spectrophotometry on a NanoDrop ND-1000 spectrophotometer (Labtech, France). .. RNA integrity was verified using the RNA-6000 NanoLabChip kit combined with a 2100 Bioanalyser (Agilent Technologies).

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: .. RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK). ..

Article Title: Coupling transcriptional activation of CRISPR–Cas system and DNA repair genes by Csa3a in Sulfolobus islandicus
Article Snippet: .. The quality and quantity of purified total RNA were determined by measuring the absorbance at 260 and 280 nm, using a NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. Total RNA integrity was verified by electrophoresis on a 1.5% agarose gel.

Article Title: The proteomes of transcription factories containing RNA polymerases I, II or III
Article Snippet: .. Protein concentrations were monitored using a Nanodrop ND-1000 spectrophotometer (LabTech). ..

Article Title: Small RNA-mediated Cry toxin silencing allows Bacillus thuringiensis to evade Caenorhabditis elegans avoidance behavioral defenses
Article Snippet: .. The purified total RNA was quantified by NanoDrop ND-1000 spectrophotometer (Labtech, Wilmington, MA, USA). .. The qRT-PCR analysis of cry5Ba and BtsR1 was carried out using two-step methods with the primers listed in .

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: .. The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France). ..

Sampling:

Article Title: Common FXIII and Fibrinogen Polymorphisms in Abdominal Aortic Aneurysms
Article Snippet: Paragraph title: Blood sampling and DNA extraction ... DNA concentration was measured using the 260/280 nm optical density ratio using a Nanodrop ND-1000 spectrophotometer (LabTech International, Uckfield, UK).

Article Title: Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes, et al. Ecotypic differentiation under farmers' selection: Molecular insights into the domestication of Pachyrhizus Rich. ex DC. (Fabaceae) in the Peruvian Andes
Article Snippet: Paragraph title: Sampling, genotyping, and DNA sequencing ... Purified DNA was eluted in a final volume of 200 μl, and final concentration was checked using a NanoDrop ND‐1000 spectrophotometer (Labtech, Palaiseau, France).

Concentration Assay:

Article Title: Common FXIII and Fibrinogen Polymorphisms in Abdominal Aortic Aneurysms
Article Snippet: .. DNA concentration was measured using the 260/280 nm optical density ratio using a Nanodrop ND-1000 spectrophotometer (LabTech International, Uckfield, UK). .. Real Time Polymerase Chain Reaction TaqMan single nucleotide polymorphism (SNP) Genotyping Assays (Life Technologies, Paisley, UK) were used to identify each of the polymorphisms (FXIII A Val34Leu (rs5985), FXIII B His95Arg (rs6003), Splice Variant (intron K nt29576C-G) (rs12134960), Fib-β Arg448Lys (rs4220) and Fib-α Thr312Ala (rs6050)).

Article Title: Balance between Estrogens and Proinflammatory Cytokines Regulates Chemokine Production Involved in Thymic Germinal Center Formation
Article Snippet: .. The quality and concentration of RNA were analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France). .. RNA samples presenting a minimal ratio of 1.9 and 2 for respectively 260/280 and 260/230 were also controlled on a denaturing agarose gel.

Article Title: Cultured Human Thymic-Derived Cells Display Medullary Thymic Epithelial Cell Phenotype and Functionality
Article Snippet: .. The concentration of RNA was analyzed with a NanoDrop ND-1000 spectrophotometer (LabTech, Palaiseau, France). ..

Article Title: Nasal airway epithelial cell IL-6 and FKBP51 gene expression and steroid sensitivity in asthmatic children
Article Snippet: .. RNA was then resuspended in RNAse-free H2 0 and RNA concentration was determined by spectrophotometry on a NanoDrop ND-1000 spectrophotometer (Labtech, France). .. RNA integrity was verified using the RNA-6000 NanoLabChip kit combined with a 2100 Bioanalyser (Agilent Technologies).

Article Title: The effect of the timing of exposure to Campylobacter jejuni on the gut microbiome and inflammatory responses of broiler chickens
Article Snippet: .. RNA quality and concentration were assessed using Nanodrop ND-1000 Spectrophotometer (Labtech International Ltd., Uckfield, UK). ..

Article Title: Resolution of quantitative resistance to clubroot into QTL-specific metabolic modules
Article Snippet: .. The DNA concentration was normalized using a Nanodrop ND-1000 Spectrophotometer (Labtech, Palaiseau, France). ..

Gel Extraction:

Article Title: Gut microbiome associated with chemotherapy-induced diarrhea from the CapeOX regimen as adjuvant chemotherapy in resected stage III colorectal cancer
Article Snippet: The nanodrop ND-1000 spectrophotometer (LabTech, Washington, DC, USA) with the absorbances at 260 nm and 280 nm (A260/A280) was used to determine the quality and quantity of the purified DNA. .. PCR amplifications were performed in triplicate in a 25 μL mixture containing 5 μL of DNA template, 2 μL of Nextera XT Index Primer 1 (10 M), 2 μL of Nextera XT Index Primer 2 (10 M) and 16 μL ddH2 O. Amplicons were extracted from 2% agarose gels, purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using QuantiFluor™-ST (Promega, U.S.) according to the manufacturer’s instructions.

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    LabTech Inc nanodrop nd 1000 spectrophometer
    Nanodrop Nd 1000 Spectrophometer, supplied by LabTech Inc, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanodrop nd 1000 spectrophometer/product/LabTech Inc
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nanodrop nd 1000 spectrophometer - by Bioz Stars, 2020-01
    77/100 stars
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