ncoi xhoi restricted pfastbac hta  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ncoi xhoi restricted pfastbac hta
    Ncoi Xhoi Restricted Pfastbac Hta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncoi xhoi restricted pfastbac hta/product/Thermo Fisher
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi xhoi restricted pfastbac hta - by Bioz Stars, 2020-04
    87/100 stars

    Related Products / Commonly Used Together

    ncoi/xhoi fragment
    pma48

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    Related Articles

    Introduce:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: Baculovirus shuttle plasmid pMA326 was constructed by PCR amplification of pMA323 with primer pair UL51-FLAG-BamHI/UL51-FLAG-ER1R (which introduce flanking BamHI and EcoRI sites) and ligation of the BamHI/EcoRI-digested PCR product into BamHI/EcoRI-digested pFastbac1. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Clone Assay:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: The UL56 coding sequence was assembled by PCR amplification of HCMV strain AD169 DNA using primer pairs MOL60/MOL64 and MOL59/MOL65 and cloning of the products into pGEM-T vector (Promega) to generate plasmids pMA48 and pMA49, respectively. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Amplification:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: The UL56 coding sequence was assembled by PCR amplification of HCMV strain AD169 DNA using primer pairs MOL60/MOL64 and MOL59/MOL65 and cloning of the products into pGEM-T vector (Promega) to generate plasmids pMA48 and pMA49, respectively. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Ligation:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: Baculovirus shuttle plasmid pMA326 was constructed by PCR amplification of pMA323 with primer pair UL51-FLAG-BamHI/UL51-FLAG-ER1R (which introduce flanking BamHI and EcoRI sites) and ligation of the BamHI/EcoRI-digested PCR product into BamHI/EcoRI-digested pFastbac1. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Isolation:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51. .. The cDNA of UL89 was prepared by RT-PCR of RNA isolated from MRC-5 cells 72 hours post infection with HCMV strain AD169 using ULTRASPEC RNA (BiotecX).

    Infection:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51. .. The cDNA of UL89 was prepared by RT-PCR of RNA isolated from MRC-5 cells 72 hours post infection with HCMV strain AD169 using ULTRASPEC RNA (BiotecX).

    Sequencing:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: The UL56 coding sequence was assembled by PCR amplification of HCMV strain AD169 DNA using primer pairs MOL60/MOL64 and MOL59/MOL65 and cloning of the products into pGEM-T vector (Promega) to generate plasmids pMA48 and pMA49, respectively. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51. .. The cDNA of UL89 was prepared by RT-PCR of RNA isolated from MRC-5 cells 72 hours post infection with HCMV strain AD169 using ULTRASPEC RNA (BiotecX).

    Polymerase Chain Reaction:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: The UL56 coding sequence was assembled by PCR amplification of HCMV strain AD169 DNA using primer pairs MOL60/MOL64 and MOL59/MOL65 and cloning of the products into pGEM-T vector (Promega) to generate plasmids pMA48 and pMA49, respectively. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Construct:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: Baculovirus shuttle plasmid pMA326 was constructed by PCR amplification of pMA323 with primer pair UL51-FLAG-BamHI/UL51-FLAG-ER1R (which introduce flanking BamHI and EcoRI sites) and ligation of the BamHI/EcoRI-digested PCR product into BamHI/EcoRI-digested pFastbac1. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Expressing:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: Paragraph title: Plasmid and baculovirus expression vector construction ... An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Modification:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: FLAG-UL51 expression plasmid pMA323 was constructed by PCR amplification of pMA320 with primer pair UL51-FLAG-H3F/UL51-FLAG-ER1R (which introduce flanking HindIII and EcoRI sites) and ligation of the HindIII/EcoRI-digested PCR product into HindIII/EcoRI-digested pcDNA-2FLAGAB, a modification of pcDNA3 to express N-terminal FLAG fusions [ ]. .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51.

    Plasmid Preparation:

    Article Title: Changes in subcellular localization reveal interactions between human cytomegalovirus terminase subunits
    Article Snippet: .. An NcoI/XhoI fragment of pMA48 was then ligated into NcoI/XhoI-restricted pFastbac-HTa (Invitrogen) to make plasmid pMA50 and an NcoI fragment from pMA48 was inserted into NcoI-digested pMA50 make plasmid pMA51. .. An XhoI/SalI fragment from pMA49 was then inserted into XhoI-digested pMA51 to make baculovirus shuttle plasmid pMA52.