ncoi high fidelity hf  (New England Biolabs)


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  • 99
    Name:
    NcoI HF
    Description:
    NcoI HF 5 000 units
    Catalog Number:
    r3193l
    Price:
    261
    Size:
    5 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ncoi high fidelity hf
    NcoI HF
    NcoI HF 5 000 units
    https://www.bioz.com/result/ncoi high fidelity hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi high fidelity hf - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The Asp20-to-Asn Substitution in the Response Regulator AdeR Leads to Enhanced Efflux Activity of AdeB in Acinetobacter baumannii
    Article Snippet: .. DNA restriction was performed using the endonucleases EcoRI-HF, NcoI-HF, and ScaI-HF (New England BioLabs), and cloning was performed according to the manufacturer's recommendations using the Quick Ligation kit (New England BioLabs). .. PCR products were phosphorylated and restricted plasmids dephosphorylated using T4 polynucleotide kinase and Antarctic Phosphatase (New England BioLabs), respectively.

    Luciferase:

    Article Title: Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsy
    Article Snippet: .. 5′-UTRs with WT, transversion, and deletion sequences (see also ) were synthesized by Macrogen and subcloned into the pGL3-promoter vector (Promega, E1761) between the SV40 promoter and the firefly luciferase open reading frame using HindIII (New England BioLabs, R3104) and NcoI (New England BioLabs, R3193). ..

    Agarose Gel Electrophoresis:

    Article Title: Analysis of the Lotus japonicus nuclear pore NUP107-160 subcomplex reveals pronounced structural plasticity and functional redundancy
    Article Snippet: .. PCR products were digested with restriction enzymes (NEB) NcoI-HF for NUP85 and RsaI for NUP133 and the products were separated on a 3% agarose gel. ..

    Ligation:

    Article Title: The Asp20-to-Asn Substitution in the Response Regulator AdeR Leads to Enhanced Efflux Activity of AdeB in Acinetobacter baumannii
    Article Snippet: .. DNA restriction was performed using the endonucleases EcoRI-HF, NcoI-HF, and ScaI-HF (New England BioLabs), and cloning was performed according to the manufacturer's recommendations using the Quick Ligation kit (New England BioLabs). .. PCR products were phosphorylated and restricted plasmids dephosphorylated using T4 polynucleotide kinase and Antarctic Phosphatase (New England BioLabs), respectively.

    Synthesized:

    Article Title: Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsy
    Article Snippet: .. 5′-UTRs with WT, transversion, and deletion sequences (see also ) were synthesized by Macrogen and subcloned into the pGL3-promoter vector (Promega, E1761) between the SV40 promoter and the firefly luciferase open reading frame using HindIII (New England BioLabs, R3104) and NcoI (New England BioLabs, R3193). ..

    Purification:

    Article Title: Mutants of Cre recombinase with improved accuracy
    Article Snippet: .. The purified plasmids were digested for 20 min at 37 °C in 60 μL reactions containing all of the collected DNA and 20 U of both ScaI-HF and NcoI-HF (both from New England Biolabs) in NEBuffer 4 (1 mM DTT, 50 mM potassium acetate, 20 mM tris-acetate, 10 mM magnesium acetate, pH 7.9). .. The digests quantified on 1% agarose gels following purification using the QIAprep Spin Miniprep kit.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Impact of tobacco-specific nitrosamine–derived DNA adducts on the efficiency and fidelity of DNA replication in human cells
    Article Snippet: .. For PAGE analysis, 150 ng of the PCR fragments were treated with 5 units of NcoI-HF and 1 unit of shrimp alkaline phosphatase (SAP) at 37 °C in 10 μl of NEBuffer 3 (New England Biolabs) for 1 h, followed by heating at 80 °C for 20 min to deactivate the SAP. ..

    Polymerase Chain Reaction:

    Article Title: Analysis of the Lotus japonicus nuclear pore NUP107-160 subcomplex reveals pronounced structural plasticity and functional redundancy
    Article Snippet: .. PCR products were digested with restriction enzymes (NEB) NcoI-HF for NUP85 and RsaI for NUP133 and the products were separated on a 3% agarose gel. ..

    Article Title: Impact of tobacco-specific nitrosamine–derived DNA adducts on the efficiency and fidelity of DNA replication in human cells
    Article Snippet: .. For PAGE analysis, 150 ng of the PCR fragments were treated with 5 units of NcoI-HF and 1 unit of shrimp alkaline phosphatase (SAP) at 37 °C in 10 μl of NEBuffer 3 (New England Biolabs) for 1 h, followed by heating at 80 °C for 20 min to deactivate the SAP. ..

    Plasmid Preparation:

    Article Title: Directed Evolution of Improved Zinc Finger Methyltransferases
    Article Snippet: .. Plasmid DNA (500 ng) was digested with NcoI-HF (10 units) and either FspI (2.5 units) or SnaBI (2.5 units) in 1X NEBuffer 4 for over one hour at 37°C. ..

    Article Title: Dynactin1 depletion leads to neuromuscular synapse instability and functional abnormalities
    Article Snippet: .. These three sequences were inserted after the ubc intron of the pT1UciMP Tol1 destination vector opened by restriction digest with NcoI-HF (NEB) to obtain 14xUAS:ubc-hDCTN1-eGFP-E2A-tagRFPCaax-pA. .. 14xUAS:ubc-ngfra-eGFP-E2A-tagRFPCaax-pA Similar to the construct above, the cDNA sequence of the ngfra zebrafish gene (ENSDARG00000088708) coding for the p75 trophic receptor was amplified from zebrafish cDNA via PCR and combined with eGFP and E2A-tagRFPCaax into the pT1UciMP Tol1 destination vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) to obtain 14xUAS:ubc-ngfra-eGFP-E2A-tagRFPCaax-pA.

    Article Title: Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsy
    Article Snippet: .. 5′-UTRs with WT, transversion, and deletion sequences (see also ) were synthesized by Macrogen and subcloned into the pGL3-promoter vector (Promega, E1761) between the SV40 promoter and the firefly luciferase open reading frame using HindIII (New England BioLabs, R3104) and NcoI (New England BioLabs, R3193). ..

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  • 99
    New England Biolabs ncoi high fidelity hf
    Ncoi High Fidelity Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncoi high fidelity hf/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi high fidelity hf - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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