ncoi ecori digested pdrive meif4a1 vector  (InvivoGen)

 
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    Name:
    pDRIVE meIF4A1
    Description:
    pDRIVE bearing mouse eIF4A1 promoter LacZ
    Catalog Number:
    pdrive-meif4a1
    Price:
    None
    Size:
    20 µg
    Category:
    pDRIVE eIF4A1 Ubiquitous Promoters Promoters Genes Promoters
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    Structured Review

    InvivoGen ncoi ecori digested pdrive meif4a1 vector
    pDRIVE bearing mouse eIF4A1 promoter LacZ
    https://www.bioz.com/result/ncoi ecori digested pdrive meif4a1 vector/product/InvivoGen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi ecori digested pdrive meif4a1 vector - by Bioz Stars, 2020-07
    85/100 stars

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    Related Articles

    Modification:

    Article Title: Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice
    Article Snippet: .. The 6.8-kb pDrive-SV40-bAlb and 5.3-kb pDrive meIF4A1 (InvivoGen), the 5.2-kb pSVβ-gal (Promega), and 8.6-kb pcDNA3.1/His/ lac Z (Invitrogen) were used without modification. .. All DNA was prepared endotoxin-free using QIAGEN kits according to the recommended protocol.

    Clone Assay:

    Article Title: Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice
    Article Snippet: .. The mouse eukaryotic initiation factor 4A1 promoter–driven (IF-driven) SB 10 and the pT2/IF SB 10 were generated by blunt-end cloning of SB 10 CDS into the NcoI/EcoRI-digested pDrive-meIF4A1 vector (InvivoGen). .. This was followed by ligation of the 1.8-kb PacI SB 10 fragment from the IF promoter using the SV40 poly(A) region into the NarI site of the second version of the Tn (pT2) ( ). pT2/CAGGS-BΔcFVIII//IF SB 10 was generated by blunt-end ligation of the 7.2-kb SalI-MluI transgene expression cassette containing the CAGGS promoter, CDS, and poly(A) signal from the CAGGS vector ( ) into the NheI cloning site between the IR/DRs.

    Generated:

    Article Title: Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice
    Article Snippet: .. The mouse eukaryotic initiation factor 4A1 promoter–driven (IF-driven) SB 10 and the pT2/IF SB 10 were generated by blunt-end cloning of SB 10 CDS into the NcoI/EcoRI-digested pDrive-meIF4A1 vector (InvivoGen). .. This was followed by ligation of the 1.8-kb PacI SB 10 fragment from the IF promoter using the SV40 poly(A) region into the NarI site of the second version of the Tn (pT2) ( ). pT2/CAGGS-BΔcFVIII//IF SB 10 was generated by blunt-end ligation of the 7.2-kb SalI-MluI transgene expression cassette containing the CAGGS promoter, CDS, and poly(A) signal from the CAGGS vector ( ) into the NheI cloning site between the IR/DRs.

    Plasmid Preparation:

    Article Title: Nanocapsule-delivered Sleeping Beauty mediates therapeutic Factor VIII expression in liver sinusoidal endothelial cells of hemophilia A mice
    Article Snippet: .. The mouse eukaryotic initiation factor 4A1 promoter–driven (IF-driven) SB 10 and the pT2/IF SB 10 were generated by blunt-end cloning of SB 10 CDS into the NcoI/EcoRI-digested pDrive-meIF4A1 vector (InvivoGen). .. This was followed by ligation of the 1.8-kb PacI SB 10 fragment from the IF promoter using the SV40 poly(A) region into the NarI site of the second version of the Tn (pT2) ( ). pT2/CAGGS-BΔcFVIII//IF SB 10 was generated by blunt-end ligation of the 7.2-kb SalI-MluI transgene expression cassette containing the CAGGS promoter, CDS, and poly(A) signal from the CAGGS vector ( ) into the NheI cloning site between the IR/DRs.

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  • 85
    InvivoGen ncoi ecori digested pdrive meif4a1 vector
    Ncoi Ecori Digested Pdrive Meif4a1 Vector, supplied by InvivoGen, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncoi ecori digested pdrive meif4a1 vector/product/InvivoGen
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncoi ecori digested pdrive meif4a1 vector - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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