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xpa  (Novus Biologicals)
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Novus Biologicals xpa
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Xpa, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with specific antibodies against XPA and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.

Journal: Cancer Epidemiology Biomarkers & Prevention

Article Title: Expression of Nucleotide Excision Repair Proteins in Lymphocytes as a Marker of Susceptibility to Squamous Cell Carcinomas of the Head and Neck

doi: 10.1158/1055-9965.epi-05-0101

Figure Lengend Snippet: Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with specific antibodies against XPA and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.

Article Snippet: We used mouse anti-human monoclonal or anti-goat or anti-rabbit polyclonal antibodies against XPD and XPG (Santa Cruz Biotechnology, Santa Cruz, CA); XPA, XPC, and XPF (Abcam, Cambridge, MA); ERCC1 (Novus Biological, Littleton, CO); and h-actin (Sigma).

Techniques: Microarray, Expressing, Control