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mmp-3 antibody - bsa free (Bio-Techne corporation)

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Bio-Techne corporation mmp-3 antibody - bsa free
Mmp 3 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mmp-3 antibody - bsa free/product/Bio-Techne corporation
Average 91 stars, based on 13 article reviews

mmp-3 antibody - bsa free - by Bioz Stars, 2026-04

91/100 stars

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Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Primer.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

Univariate and multivariate cox regression.

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Univariate and multivariate cox regression.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

The mRNA levels of hub genes. ( A ) The gene expression levels of hub genes in HCT116 and FHC cell. The FHC cells are normal human intestinal epithelial cells, while HCT116 cells are human colorectal carcinoma cells. ( B ) The gene expression levels of hub genes in CRC tissue and Noncancerous tissue from human patients. ( C ) Representative images and statistics of IHC staining for MMP3, C5AR1, TIMP1, ADAM8 in colon tissues and CRC from the Human Protein Atlas dataset. ( D ) The protein expression levels of MMP3 (

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: The mRNA levels of hub genes. ( A ) The gene expression levels of hub genes in HCT116 and FHC cell. The FHC cells are normal human intestinal epithelial cells, while HCT116 cells are human colorectal carcinoma cells. ( B ) The gene expression levels of hub genes in CRC tissue and Noncancerous tissue from human patients. ( C ) Representative images and statistics of IHC staining for MMP3, C5AR1, TIMP1, ADAM8 in colon tissues and CRC from the Human Protein Atlas dataset. ( D ) The protein expression levels of MMP3 ("F" represents FHC cells, and "H" represents HCT116 cells), ADAM8, TIMP1, and C5AR1 were assessed in FHC cells and HCT116 cells, with GAPDH serving as a reference. Statistical significance levels were denoted as *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques: Expressing, Immunohistochemistry

Illustration of the roles of SPP1, C5AR1, MMP3, TIMP1, and ADAM8 in the progression of colorectal cancer. Created by biorender.

Journal: Scientific Reports

Article Title: Identification of a novel macrophage-related prognostic signature in colorectal cancer

doi: 10.1038/s41598-024-53207-9

Figure Lengend Snippet: Illustration of the roles of SPP1, C5AR1, MMP3, TIMP1, and ADAM8 in the progression of colorectal cancer. Created by biorender.

Article Snippet: Antibody against MMP3 was obtained from Bio-Techne.

Techniques:

Figure 2: The eﬀect of miR-101-3p upregulation or inhibition on NP cells. (a) The miR-101-3p transfected NP cells conﬁrmed by Cy3, scale bar = 25 μm. (b) The miR-101-3p expression level evaluated by qRT-PCR at 48 h after transfection. n = 3 replicates per group. (c) The cell apoptosis was accessed by ﬂow cytometry. (d) Cell viability by CCK-8. (e) The Col II and MMP 3 expression was detected by immunoﬂuorescence. Scale bar = 50 μm. (f) The expression levels of MMP3 and Col II proteins were detected by western blots. Quantitative analysis is shown on the right. Values presented as mean ± SD. ∗∗∗p < 0:001. NP: nucleus pulposus.

Journal: Oxidative medicine and cellular longevity

Article Title: The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway.

doi: 10.1155/2021/1073458

Figure Lengend Snippet: Figure 2: The eﬀect of miR-101-3p upregulation or inhibition on NP cells. (a) The miR-101-3p transfected NP cells conﬁrmed by Cy3, scale bar = 25 μm. (b) The miR-101-3p expression level evaluated by qRT-PCR at 48 h after transfection. n = 3 replicates per group. (c) The cell apoptosis was accessed by ﬂow cytometry. (d) Cell viability by CCK-8. (e) The Col II and MMP 3 expression was detected by immunoﬂuorescence. Scale bar = 50 μm. (f) The expression levels of MMP3 and Col II proteins were detected by western blots. Quantitative analysis is shown on the right. Values presented as mean ± SD. ∗∗∗p < 0:001. NP: nucleus pulposus.

Article Snippet: 5% skim milk was used to block the membranes for 2 h at room temperature, and incubate membranes overnight at 4°C with anti-STC1 antibody (AB_2608564, 1 : 1000, Invitrogen, USA), anti-VEGF antibody (AB_2212682, 1 : 1000, Invitrogen, USA), anti-MMP3 antibody (NB100-91878, 1 : 1000, Novus, USA), anti-Col II antibody (NB600-844, 1 : 1000, Novus, USA), anti-IL-1β antibody (AB_468396, 1 : 1000, Invitrogen, USA), and anti-β-actin antibody (abs118937, 1 : 3000, Absin, China) in 5% bovine serum albumin (BSA) in TBS-T overnight at 4°C.

Techniques: Inhibition, Transfection, Expressing, Quantitative RT-PCR, Cytometry, CCK-8 Assay, Western Blot

Expression of kidney: (a) MMP2; (b) MMP3; (c) MMP9; (d) TIMP2. Middle boxes represent the inter-quartile range. Whiskers represent the lower and upper scores. Stars indicate the presence of outliers in the data set. A number indicates the serial number of the animal which has the outlying score. Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment).

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Expression of kidney: (a) MMP2; (b) MMP3; (c) MMP9; (d) TIMP2. Middle boxes represent the inter-quartile range. Whiskers represent the lower and upper scores. Stars indicate the presence of outliers in the data set. A number indicates the serial number of the animal which has the outlying score. Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment).

Article Snippet: Slides were then incubated for 60 min with the antibodies MMP2 (mouse monoclonal, Novus Biological, NB 200-114) at 1:300 dilution, MMP3 (rabbit polyclonal, Nobus Biologicals, NB100-91878) at 1:70 dilution, MMP9 (rabbit polyclonal, Novus Biologicals, NBP1-57940) at 1:250 dilution, and TIMP2 (mouse monoclonal, Novus Biologicals, NBP2-01573) at 1:300 dilution.

Techniques: Expressing

Expression of MMP2 and MMP3 in kidney for all study groups and the results of the statistical analysis <xref ref-type=

* ." width="100%" height="100%">

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Expression of MMP2 and MMP3 in kidney for all study groups and the results of the statistical analysis * .

Techniques: Expressing

Photomicrographs of MMP2 (a, b), MMP3 (c, d) and MMP9 (e, f) in kidney of control (a,c,e) and silibinin (b,d,f) groups at 240 min (x200).

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Photomicrographs of MMP2 (a, b), MMP3 (c, d) and MMP9 (e, f) in kidney of control (a,c,e) and silibinin (b,d,f) groups at 240 min (x200).

Techniques: Control

Photomicrographs of MMP2 (a, b) , MMP3 (c, d) , MMP9 (e, f) and TIMP2 (g, h) in lung of control (a, c, e, g) and silibinin (b, d, f, h) groups at 240 min for MMP2, MMP3 and MMP9 and 180 min for TIMP2 (x200).

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Photomicrographs of MMP2 (a, b) , MMP3 (c, d) , MMP9 (e, f) and TIMP2 (g, h) in lung of control (a, c, e, g) and silibinin (b, d, f, h) groups at 240 min for MMP2, MMP3 and MMP9 and 180 min for TIMP2 (x200).

Techniques: Control

Expression of lung: (a) MMP2; (b) MMP3; (c) MMP9; (d) TIMP2. Middle boxes represent the inter-quartile range. Whiskers represent the lower and upper scores. Stars indicate the presence of outliers in the data set. A number indicates the serial number of the animal which has the outlying score. Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment).

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Expression of lung: (a) MMP2; (b) MMP3; (c) MMP9; (d) TIMP2. Middle boxes represent the inter-quartile range. Whiskers represent the lower and upper scores. Stars indicate the presence of outliers in the data set. A number indicates the serial number of the animal which has the outlying score. Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment).

Techniques: Expressing

Expression of MMP2 and MMP3 in lung for all study groups and the results of the statistical analysis <xref ref-type=

* ." width="100%" height="100%">

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Expression of MMP2 and MMP3 in lung for all study groups and the results of the statistical analysis * .

Techniques: Expressing

Box and Whiskers plot for gene expression of MMPs in kidneys after liver IRI in Wistar rats: ΔCt MMP2 (a) , ΔCt MMP3 (b) , ΔCt MMP9 (c) and ΔCt TIMP2 (d) . Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment). Statistically significant difference p<0.05:

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Box and Whiskers plot for gene expression of MMPs in kidneys after liver IRI in Wistar rats: ΔCt MMP2 (a) , ΔCt MMP3 (b) , ΔCt MMP9 (c) and ΔCt TIMP2 (d) . Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment). Statistically significant difference p<0.05:

Techniques: Gene Expression

Box and Whiskers plot for gene expression of MMPs in lungs after liver IRI in Wistar rats: ΔCt MMP2 (a) , ΔCt MMP3 (b) , ΔCt MMP9 (c) and ΔCt TIMP2 (d) . Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment). Statistically significant difference p<0.05.

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Box and Whiskers plot for gene expression of MMPs in lungs after liver IRI in Wistar rats: ΔCt MMP2 (a) , ΔCt MMP3 (b) , ΔCt MMP9 (c) and ΔCt TIMP2 (d) . Kruskal-Wallis test (Dunn’s test with Bonferroni adjustment). Statistically significant difference p<0.05.

Techniques: Gene Expression

Relative quantity (R=2 -ΔΔCt ) values for the effect of silibinin on MMP2, MMP3, MMP9 and TIMP2 gene expression in kidney and lung after hepatic IRI.

Journal: Acta Cirúrgica Brasileira

Article Title: Effect of silibinin on the expression of MMP2, MMP3, MMP9 and TIMP2 in kidney and lung after hepatic ischemia/reperfusion injury in an experimental rat model

doi: 10.1590/ACB360904

Figure Lengend Snippet: Relative quantity (R=2 -ΔΔCt ) values for the effect of silibinin on MMP2, MMP3, MMP9 and TIMP2 gene expression in kidney and lung after hepatic IRI.

Techniques: Gene Expression

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