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nnos antibody  (Bio-Techne corporation)


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    Bio-Techne corporation nnos antibody
    Nnos Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nnos antibody/product/Bio-Techne corporation
    Average 92 stars, based on 14 article reviews
    nnos antibody - by Bioz Stars, 2026-04
    92/100 stars

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    Figure 2. Summary of identified brain RAS differences in Alzheimer’s dementia. ACE = angiotensin-converting enzyme; Ang = angiotensin; AP-A = aminopeptidase A; AP-N = aminopeptidase N; AT1R = angiotensin II type 1 receptor; AT2R = angiotensin II type 2 <t>receptor;</t> <t>AT4R</t> = angiotensin IV receptor; eNOS = endothelial nitric oxide synthase; <t>nNOS</t> = neuronal nitric oxide synthase; pERK = phosphorylated extracellular signal-regulated kinases.
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    Confocal images of immunostained brain sections from (a) VIP-cre, (b) PV-cre, <t>(c)</t> <t>SOM-cre</t> and (d) <t>NOS-cre</t> mice. For each mouse, larger field-of-view images are shown on the left panels and zoomed images on the right panels (white rectangle on left panels shows zoomed location for images on the right panels). The top panels show staining for the model of interest (red), middle panels show staining for ChR2-YFP (green) and the bottom panels show color merge images. White arrows show location-matched cells between zoomed panels from the same mice.
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    Image Search Results


    Figure 2. Summary of identified brain RAS differences in Alzheimer’s dementia. ACE = angiotensin-converting enzyme; Ang = angiotensin; AP-A = aminopeptidase A; AP-N = aminopeptidase N; AT1R = angiotensin II type 1 receptor; AT2R = angiotensin II type 2 receptor; AT4R = angiotensin IV receptor; eNOS = endothelial nitric oxide synthase; nNOS = neuronal nitric oxide synthase; pERK = phosphorylated extracellular signal-regulated kinases.

    Journal: The journals of gerontology. Series A, Biological sciences and medical sciences

    Article Title: Higher Angiotensin II Type 1 Receptor Levels and Activity in the Postmortem Brains of Older Persons with Alzheimer's Dementia.

    doi: 10.1093/gerona/glab376

    Figure Lengend Snippet: Figure 2. Summary of identified brain RAS differences in Alzheimer’s dementia. ACE = angiotensin-converting enzyme; Ang = angiotensin; AP-A = aminopeptidase A; AP-N = aminopeptidase N; AT1R = angiotensin II type 1 receptor; AT2R = angiotensin II type 2 receptor; AT4R = angiotensin IV receptor; eNOS = endothelial nitric oxide synthase; nNOS = neuronal nitric oxide synthase; pERK = phosphorylated extracellular signal-regulated kinases.

    Article Snippet: The following antibodies were used: AT1R (sc-515884; Santa Cruz Biotechnology, Santa Cruz, CA) (25), AT2R (sc-9040; Santa Cruz, CA) (26,27), AT4R (HPA043642; Sigma-Aldrich, St Louis, MO) (28), nNOS (NB100-858; Novus Biologicals, Littleton, CO) (29), eNOS (NB300-500; Novus, CO) (30), phosphorylated and total ERK (9101 and 9102; Cell Signaling Technology, Beverly, MA) (31), and PGC1α (NBP1-04676; Novus, CO) (32).

    Techniques:

    Confocal images of immunostained brain sections from (a) VIP-cre, (b) PV-cre, (c) SOM-cre and (d) NOS-cre mice. For each mouse, larger field-of-view images are shown on the left panels and zoomed images on the right panels (white rectangle on left panels shows zoomed location for images on the right panels). The top panels show staining for the model of interest (red), middle panels show staining for ChR2-YFP (green) and the bottom panels show color merge images. White arrows show location-matched cells between zoomed panels from the same mice.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Optogenetic assessment of VIP, PV, SOM and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex

    doi: 10.1177/0271678X19870105

    Figure Lengend Snippet: Confocal images of immunostained brain sections from (a) VIP-cre, (b) PV-cre, (c) SOM-cre and (d) NOS-cre mice. For each mouse, larger field-of-view images are shown on the left panels and zoomed images on the right panels (white rectangle on left panels shows zoomed location for images on the right panels). The top panels show staining for the model of interest (red), middle panels show staining for ChR2-YFP (green) and the bottom panels show color merge images. White arrows show location-matched cells between zoomed panels from the same mice.

    Article Snippet: The following primary antibodies were then applied for 72 h at 4°C: YFP (1:500, chicken anti-GFP polyclonal, ab13970, Abcam Inc., Cambridge, MA), Parvalbumin (1:500 rabbit anti-parvalbumin polyclonal, ab11427 Abcam), VIP (1:600, rabbit anti-VIP polyclonal, Immunostar, Hudson, WI), SOM (1:600, rabbit anti-SST polyclonal, HPA019472 Sigma-Aldrich, St. Loius, MO), NOS (1:500, goat anti-NOS1 polyclonal, NB100-858, Novus Biologicals, LLC, Centennial, CO).

    Techniques: Staining

    Immunostaining summary of the number of neurons expressing ChR2-YFP (YFP-positive or YFP+) in the different inhibitory neuron types of interest (INTI).

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Optogenetic assessment of VIP, PV, SOM and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex

    doi: 10.1177/0271678X19870105

    Figure Lengend Snippet: Immunostaining summary of the number of neurons expressing ChR2-YFP (YFP-positive or YFP+) in the different inhibitory neuron types of interest (INTI).

    Article Snippet: The following primary antibodies were then applied for 72 h at 4°C: YFP (1:500, chicken anti-GFP polyclonal, ab13970, Abcam Inc., Cambridge, MA), Parvalbumin (1:500 rabbit anti-parvalbumin polyclonal, ab11427 Abcam), VIP (1:600, rabbit anti-VIP polyclonal, Immunostar, Hudson, WI), SOM (1:600, rabbit anti-SST polyclonal, HPA019472 Sigma-Aldrich, St. Loius, MO), NOS (1:500, goat anti-NOS1 polyclonal, NB100-858, Novus Biologicals, LLC, Centennial, CO).

    Techniques: Immunostaining, Expressing

    Average CBF changes measured by LDF in response to photo-stimulation for 1 s at 5 Hz and various pulse widths (30 ms in blue traces, 10 ms in red traces, 2 ms in green traces), as well as whisker stimulation (black traces) in VIP-cre (a), PV-cre (c), SOM-cre (e) and NOS-cre (g) mice. Similarly, average CBF changes in response to photo-stimulation and whisker for 4 s in VIP-cre (b), PV-cre (d), SOM-cre (f) and NOS-cre (h) mice. The photo-stimulus amplitude was fixed to 1 mW. The shaded blue rectangle denotes the photo-stimulation period. For reference, the average time-to-peak for CBF responses evoked by 1-s whisker stimulation were 3.0 ± 2.0, 2.4 ± 1.1, 2.7 ± 1.7 and 2.4 ± 1.2 s for VIP-cre, PV-cre, SOM-cre and NOS-cre models, respectively, and 2.7 ± 1.6, 3.0 ± 1.2, 3.5 ± 2.1 and 2.4 ± 0.9 s to 4-s stimulation in these models, respectively.

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Optogenetic assessment of VIP, PV, SOM and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex

    doi: 10.1177/0271678X19870105

    Figure Lengend Snippet: Average CBF changes measured by LDF in response to photo-stimulation for 1 s at 5 Hz and various pulse widths (30 ms in blue traces, 10 ms in red traces, 2 ms in green traces), as well as whisker stimulation (black traces) in VIP-cre (a), PV-cre (c), SOM-cre (e) and NOS-cre (g) mice. Similarly, average CBF changes in response to photo-stimulation and whisker for 4 s in VIP-cre (b), PV-cre (d), SOM-cre (f) and NOS-cre (h) mice. The photo-stimulus amplitude was fixed to 1 mW. The shaded blue rectangle denotes the photo-stimulation period. For reference, the average time-to-peak for CBF responses evoked by 1-s whisker stimulation were 3.0 ± 2.0, 2.4 ± 1.1, 2.7 ± 1.7 and 2.4 ± 1.2 s for VIP-cre, PV-cre, SOM-cre and NOS-cre models, respectively, and 2.7 ± 1.6, 3.0 ± 1.2, 3.5 ± 2.1 and 2.4 ± 0.9 s to 4-s stimulation in these models, respectively.

    Article Snippet: The following primary antibodies were then applied for 72 h at 4°C: YFP (1:500, chicken anti-GFP polyclonal, ab13970, Abcam Inc., Cambridge, MA), Parvalbumin (1:500 rabbit anti-parvalbumin polyclonal, ab11427 Abcam), VIP (1:600, rabbit anti-VIP polyclonal, Immunostar, Hudson, WI), SOM (1:600, rabbit anti-SST polyclonal, HPA019472 Sigma-Aldrich, St. Loius, MO), NOS (1:500, goat anti-NOS1 polyclonal, NB100-858, Novus Biologicals, LLC, Centennial, CO).

    Techniques: Whisker Assay

    Summary of the changes in CBF evoked by photo-stimulation in the different transgenic mouse models used (VIP-cre, PV-cre, SOM-cre and NOS-cre). Error bars denote standard error. Since typical vascular responses evoked by stimulation peak several seconds after stimulation onset, we measured CBF changes 2–4 s after onset for 1-s stimulation (panels a, b, e and f) and 4–7 s after onset for 4-s stimulation (panels c, d, g and h). Significant differences from baseline are denoted by (white *, p < 0.05). Linear regression analyses were performed to determine whether significant relationships were observed with respect to the photo-stimulus pulse duration (panels e and g) and frequency (panels f and h). Significant relationships are denoted by a (black *, p < 0.05).

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Optogenetic assessment of VIP, PV, SOM and NOS inhibitory neuron activity and cerebral blood flow regulation in mouse somato-sensory cortex

    doi: 10.1177/0271678X19870105

    Figure Lengend Snippet: Summary of the changes in CBF evoked by photo-stimulation in the different transgenic mouse models used (VIP-cre, PV-cre, SOM-cre and NOS-cre). Error bars denote standard error. Since typical vascular responses evoked by stimulation peak several seconds after stimulation onset, we measured CBF changes 2–4 s after onset for 1-s stimulation (panels a, b, e and f) and 4–7 s after onset for 4-s stimulation (panels c, d, g and h). Significant differences from baseline are denoted by (white *, p < 0.05). Linear regression analyses were performed to determine whether significant relationships were observed with respect to the photo-stimulus pulse duration (panels e and g) and frequency (panels f and h). Significant relationships are denoted by a (black *, p < 0.05).

    Article Snippet: The following primary antibodies were then applied for 72 h at 4°C: YFP (1:500, chicken anti-GFP polyclonal, ab13970, Abcam Inc., Cambridge, MA), Parvalbumin (1:500 rabbit anti-parvalbumin polyclonal, ab11427 Abcam), VIP (1:600, rabbit anti-VIP polyclonal, Immunostar, Hudson, WI), SOM (1:600, rabbit anti-SST polyclonal, HPA019472 Sigma-Aldrich, St. Loius, MO), NOS (1:500, goat anti-NOS1 polyclonal, NB100-858, Novus Biologicals, LLC, Centennial, CO).

    Techniques: Transgenic Assay